• 한국약용작물학회지
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• 제22권3호
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• pp.177-187
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• 2014

We compared effects of fruit, leaf, and stem extracts from black raspberry on improvement of cholesterol and blood pressure in HepG2 and HUVEC cells, respectively. Cholesterol secretion was inhibited by water extracts of unripe fruit and stem, but not leaf of black raspberry in HepG2 cells. Also, water extracts of unripe fruit, leaf, and stem reduced HMG-CoA reductase activity. Furthermore, nitric oxide production and expression of angiotensin-converting enzyme (ACE) protein were regulated by extracts of fruit, leaf, and stem of black raspberry in HUVEC cells. Overall, the rank order according to the improving level of cholesterol and hypertension is as follows: stem > fruit > leaf. In addition, various polyphenol compounds displayed inhibitory effects of HMG-CoA reductase activity and ACE expression. Thus, these data suggested that leaf and stem as wells as fruit of black raspberry can be used as useful food resources for reduction of cholesterol and blood pressure.

• 사상체질의학회지
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• 제21권1호
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• pp.28-34
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• 2009

1. Objective This study aims to establish the concept and classification of the Gochangl(鼓脹) pathology introduced in the Sasang Constitutional Medicine literature. 2. Methods Different editions of Donguisusebowon, including the Gabo version and the Sinchuk version, were compared for differences and similarities in the concept, symptomology, pathology, and therapeutics concerning the Soyangin Gochang disease. 3. Results and Conclusion (1) In the Gabo edition, Gochang(鼓脹) is introduced to Eumheohwadongwiyeolyukran(陰虛火動胃熱肉爛) disease category of the Interior-Based Symptomatic Pattern in the "Soyangin Interior Large Intestine Disease" section and is introduced to the conditions progressed from edema included in the Exterior-Based Symptomatic Pattern in the "Articles added to the Soyangin Bladder and Large Intestine Disease" section. (2) In the Sinchuk edition, Gochang(鼓脹) is introduced ro the conditions progressed from Jungso(中消) of the Interior-Based Symptomatic Pattern in the "Soyangin Interior-heat-disease by the fever in the stomach" section and "Articles added to the Soyangin" section. (3) It is reasonable to think that Soyangin's Gochang is considered to Interior-Based Symptomatic Pattern because the Sinchuk edition is developed from the Gabo edition and Gochang is introduced to Jungso(中消) category after the distinction between Gochang and edema in the Sinchuk edition.

• 농촌계획
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• 제27권3호
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• pp.103-116
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• 2021

In this study, the characteristics of rural tourism image perceived by urban residents were analyzed through text analysis of blog data. In order to examine the images related to rural tourism, blog data written with the keyword "Gochang-gun travel" was used. LDA topic analysis, one of the text mining techniques, was used for the analysis. In the tourism image of Gochang-gun, 9 topics were derived, and 112 major places appeared. This was divided into 3 main activities and 5 object spaces through the review of keywords and the original text of blog data. As a result of the analysis, the traditional main resources of the region, Seonun mountain, Seonun temple, and Gochang-eup fortress, formed topic. On the other hand, world heritage such as dolmen and Ungok wetland did not appear as topic. In particular, the farms operated by the private sector form individual topics, and the theme farm can be seen as an important resource for tourism in Gochang-gun. Also, through the distribution of place keywords, it was possible to understand the characteristics of travel by region and the usage behavior of visitors. In the case of Gochang-gun, there was a phenomenon in which visitors were biased by region. This seems to be the result of Gochang-gun seeking to vitalize local tourism focusing on natural, ecological, and scenic resources. It is necessary to establish a plan for balanced regional development and develop other types of tourism resources. This study is different in that it identified the types and characteristics of rural tourism images in the region perceived by visitors, and the status of tourism at the regional level.

• 농촌계획
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• 제15권1호
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• pp.101-107
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• 2009

The purposes of this study were to search for the ways to activate the Gochang Blackberry Festival by examining the visitors to the festival and utilizing the results to make efficient use of the local resources and vitalize the area. The results indicate that the visitors were aware of Gochang blackberry's superiority to that of other regions in quality and commercial value but lacked in the recognition of its related products, which suggests that the Gochang government should make diverse efforts to heighten their recognition of the products. There was a great demand for improving and supplementing the local festivals ad experience programs related to Gochang blackberry. It's thus required to reinforce new experience programs and develop various programs by incorporating blackberry into other local resources. The study has its limitations since its subjects were restricted to the visitors of the Gochang Blackberry Festival and it failed to investigate the preference factors of general potential tourists with the small sample. In future study, they need to use a bigger sample of general public to generalize the research findings.

• 한국식품과학회지
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• 제46권3호
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• pp.375-383
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• 2014

본 연구는 복분자 완숙과 50% 에탄올 추출물을 이용하여 인간제대정맥내피세포주(HUVEC)에서 복분자 추출물이 NO의 생성에 미치는 영향과 ACE 단백질 발현을 조사하였으며, 본태성 고혈압 흰쥐(SHR)에서 혈압강하 효과 및 혈압의 조절과 관련 깊은 인자들(renin, AT1-R, ET-1, IL-6)의 유전자 발현 그리고 혈관조직의 변화를 조사하였다. 그 결과 복분자 완숙과 50% 에탄올 추출물은 HUVEC 세포주에서 NO의 생성을 촉진하였으며 혈관수축활성을 가지는 ACE 단백질의 발현을 억제하였고 본태성 고혈압 흰쥐에게 복분자 완숙과 에탄올 50% 추출물을 8주간 투여한 결과 혈액 내 ACE의 활성이 억제되었으며 renin 및 혈관 수축인자인 ET-1 유전자의 발현이 억제되고 염증관련 유전자인 IL-6의 발현이 억제됨을 확인하였다. 그리고 신세동맥의 두께 및 심근 호산성세포수를 감소시켜 혈관내피세포의 손상을 보호하며 심장의 병변을 경감시키는 효과가 있음을 확인하였다. 본 연구 결과 복분자 완숙과 추출물은 혈압 조절과 연관된 단백질 및 유전자의 발현과 활성을 조절하고, 혈관의 두께 및 심근의 변성을 감소시켜 혈압 강하에 도움이 되는 것으로 나타났으며 향후 심혈관계 질환을 개선시킬 수 있는 식품소재로서의 가능성이 있음을 확인하였다.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.315-320
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• 2012

The gDNA isolated from Cyclina sinensis from Gochang (GOCHANG), Incheon (INCHEON) and a Chinese site (CHINESE), were amplified by PCR. Here, the seven oligonucleotide decamer primers (BION-66, BION-68, BION-72, BION-73, BION-74, BION-76, and BION-80) were used to generate the unique shared loci to each population and shared loci by the three cyclina clam populations. As regards multiple comparisons of average bandsharing value results, cyclina clam population from Chinese (0.763) exhibited higher bandsharing values than did clam from Incheon (0.681). In this study, the dendrogram obtained by the seven decamer primers indicates three genetic clusters: cluster 1 (GOCHANG 01~GOCHANG 07), cluster 2 (INCHEON 08~INCHEON 14), cluster 3 (CHINESE 15~CHINESE 21). The shortest genetic distance that displayed significant molecular differences was between individuals 15 and 17 from the Chinese cyclina clam (0.049), while the longest genetic distance among the twenty-one cyclina clams that displayed significant molecular differences was between individuals GOCHANG no. 03 and INCHEON no. 12 (0.575). Individuals of Incheon cyclina clam population was somewhat closely related to that of Chinese cyclina clam population. In conclusion, our PCR analysis revealed a significant genetic distance among the three cyclina clam populations.

• 한국식품조리과학회지
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• 제30권6호
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• pp.792-799
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• 2014

This study was conducted to determine the optimal conditions of acetic acid fermentation of aronia using the response surface methodology (RSM). Alcohol content of aronia wine was 13.1% after alcohol fermentation of aronia fruits. The optimal conditions of acetic acid fermentation were determined by five levels of initial aronia alcohol content, initial acetic acid content, and acetic acid bacteria, using the central composite design. The acetic acid yields, residual alcohol contents, pH, and color values (L, a, b) of acetic acid fermentation products were very significantly different ($p{\leq}0.01$), and were 26.34~57.82 g/L, 0.80~1.53%, 3.19~3.43, 47.58~71.00, 31.77~51.15, and 18.45~48.57, respectively. For the results, the optimal conditions of acetic acid fermentation of aronia were 7.78% of the initial alcohol concentration, 1.58% of the initial acetic acid, and 19.39% of the acetic acid bacteria.

• 한국지구과학회지
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• 제36권6호
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• pp.533-542
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• 2015

고창 해빈은 황해의 동편, 한국 서남해 연안에 위치하며, 북쪽에서 남쪽 방향으로 동호리, 광승리, 명사십리 해빈으로 구성된다. 고창 해빈은 대조차, 개방형, 직선형 해안, 모래 저질의 특징을 보인다. 본 연구는 고창 해빈에서 표층 지형 변화와 퇴적물 집적률의 연평균과 계절 특성을 연구하였다. 2014년 겨울(2월), 봄(5월), 여름(8월), 가을(11월)과 2015년 겨울(2월)의 5계절 동안, 3개 측선을 따라 총 315개 지점에서 지형 고도를 측정하였다. 해안선에 수직인 각 측선은 30 m 간격의 21 지점으로 구성된다. 고창 해빈의 2014년 연평균 집적률은 -0.081 m/yr로 침식이 우세하였다. 지역별 연평균 집적률은 동호리 해빈 -0.091 m/yr, 광승리 해빈 -0.051 m/yr, 명사십리 해빈 -0.10 m/yr로 나타났다.

• Fisheries and Aquatic Sciences
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• 제6권1호
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• pp.13-19
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• 2003

Genomic DNA from the muscle of marsh clam (Corbicula leana) from Gochang, Muan and a Chinese site was extracted to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction (RAPD- PCR). Out of 20 primers, seven primers produced amplified fragments which were consistently polymorphic. A total of 1,246 amplified products were produced of which 530 were polymorphic $(42.5\%)$. The number of polymorphic bands produced per primer varied from 40 to 122 with an average of 75.7 in marsh clam from Gochang. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic. Also, about $4.34\%$ of total polymorphic bands were specific to marsh clam from Gochang. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, which were polymorphic. This common bands in every individuals should be diagnostic of specific strains, species and/or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of 12 specific bands. The intra-population variation was revealed in the band patterns identified by this primer. The random primer OPB-12 (CCTTGACGCA) yielded the amplified fragments which were consistently polymorphic between the marsh clams from Gochang and from Muan. This primer produced a total of 77 polymorphic bands: 31 bands from Gochang, 14 from Muan and 32 from the Chinese populations. An average of polymorphic bands were 1.8 from Gochang and 2.5 from the Chinese populations. This value obtained from the Chinese population was higher than those from the two domestic populations. Generally, the RAPD polymorphism generated by these primers may be useful as a genetic marker for strain or population identification of marsh clam.

• 한국식품영양과학회지
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• 제43권4호
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• pp.507-515
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• 2014

본 연구는 전립선암세포주인 LNCaP 세포주와 테스토스테론으로 유도된 전립선비대 백서모델에서 복분자 추출물의 전립선비대 억제 효과를 조사하였다. 첫째, 전립선암 세포주(LNCaP)에서 복분자 미숙과 추출물의 안드로겐 관련 전립선비대 유전자 억제 효과를 조사한 결과 미숙과 50% 에탄올 추출물은 안드로겐 수용체(AR)뿐만 아니라 전립선특이항원(PSA)과 5-알파 환원 효소 type 2(5AR2)의 발현을 가장 높게 억제하였다. 또한 LNCaP 세포에 DHT로 안드로겐 관련 유전자를 유도한 후 복분자 미숙과 50% 에탄올 추출물을 처리한 결과 AR과 PSA mRNA의 발현이 억제됨을 확인하였다. 둘째, 테스토스테론을 이용하여 전립선비대를 유도한 동물모델에서 복분자 미숙과 50% 에탄올 추출물을 6주간 투여한 후 전립선비대 개선 효과를 조사한 결과, 전립선비대유발군에 복분자 미숙과 추출물을 투여한 군에서 전립선 무게, 전립선 소포의 상피세포 두께 및 면적이 감소함을 확인할 수 있었고, 전립선비대 유발 호르몬인 DHT level이 감소함을 확인할 수 있었다.