• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.1
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• pp.27-38
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• 1986

This experiment was conducted to observe the changes of $CO_2$ production, firmness and lipid components of tomato fruits at the temperature of $15^{\circ}C,\;20^{\circ}C\;and\;25^{\circ}C$ each. The phenomenon of climacteric rise appeared first at $25^{\circ}C$. The firmness of tomato fruits during storage were decreased rapidly under $25^{\circ}C$ but slowly under $15^{\circ}C$. The total lipid contents of tomato fruits were decreased under $15^{\circ}C\;and\;20^{\circ}C$ until the middle stage of storage but were increased rapidly at $15^{\circ}C$ from 30 to 40 days in storage. Various changes were not observed at the neutral lipid contents of tomato fruits. But. glycolipid and phospholipid contents were increased. The results which were observed the composite fatty acid of each lipid components were a lot of palmitic acid as a saturated fatty acid and linoleic acid. linolenic acid and oleic acid as an unsaturaturad fatty acid at the all fractions which were neutral lipid, glycolipid and phospholipid. The ratios of saturated fatty acid to unsaturated fatty acid of tomato fruits during storage were generally increased at the fraction of neutral lipid. tended to decrease at the fraction of glycolipid and were almost constant at the fraction of phospholipid.

• Korean Journal of Food Science and Technology
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• v.11 no.4
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• pp.304-313
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• 1979

The compositions of the polar lipids in the free and bound lipids from four varieties of experimentally cultivated potatoes were identified and quantified by thin layer- and gas-liquid chromatographies. The results were summarized as follows : 1. The glycolipids contained in the free and bound lipids were fractionated and identified as esterified sterol glycoside, monogalactosyl diglyceride, sterol glycoside, digalactosyl diglyceride, and trigalactosyl diglyceride, of which the highest content to the total lipid quantity were 5.8% of esterified sterol glycoside in the free lipid and 6.1% of trigalactosyl diglyceride in the bound lipid. The content of monogalactosyl diglyceride in the free and bound lipids was almost the same, whereas the content of esterified sterol glycoside was higher in the free lipid than in the bound lipid, and the contents of sterol glycoside, digalactosyl diglyceride, and trigalactosyl diglyceride were higher in the bound lipid than in the free lipid on the contrary. 2. The phospholipid contained in the free and bound lipids were fractionated and identified as phosphatidyl ethanolamine, phosphatidyl glyceride, phosphatidyl choline, and phosphatidyl inositol, but the phosphatidyl glyceride was not detected in the free lipid. The highest content in the total lipid quantity was 3.3 % of phosophatidyl choline in the case of the free lipid, while 14.9 % of the phosphatidyl ethanolamine contained in the bound lipid was the highest. All other constituents of phospholipid were contained in larger quantity in the bound lipid than in the free lipid. 3. The fatty acid composition of glycolipid in the free and bound lipids was also the same as that of the total free and bound lipids. The differences were that the content of palmitic acid was higher in the glycolipid of the free lipid than in the total free lipid and the content of linoleic acid was lower in the glycolipid.

• Applied Biological Chemistry
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• v.31 no.2
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• pp.169-176
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• 1988

Lipid contents, fatty acid compositions and free sugar contents of dried ginsengs grown for different years were studied. The lipid and free sugar were separated and quantified by silicic acid column chromatography, thin layer chromatography, gas liquid chromatography and high performance liquid chromatography. Ginseng contained $1.07{\sim}67%$ crude lipid and the hightest lipid content was shown in 3 year old root. The lipid fractions obtained by silicic acid column chromatography were mainly composed of neutral lipid$(51.35{\sim}72.30%)$. The contents of phospholipid and glycolipid in total lipids were $15.03{\sim}34.59%$ and $11.83{\sim}20.72%$, respectively. As the ginseng grew, neutral lipid content increased gradually but glycolipid decreased to the half of the one year old root. Seven components of neutral lipid separated by thin layer chromatography were identified but the other two components were not able to be identified. Triglyceride content was $14.42{\sim}23.91%$, sterol ester and unidentified material(II) were $13.58{\sim}21.26%$ and $7.13{\sim}15.83%$ respectively. The major fatty acids were linoleic acid, palmitic acid, oleic acid and linolenic acid. Linoleic acid content of total and neutral lipid was $61{\sim}65%$ palmitic acid content in phospholipid was $26{\sim}39%$ and one year old root showed the hightest contents. The fatty acid composition of neutral lipid was similar to the pattern of total lipid. Linolenic acid content of glycolipid decreased gradually. Free sugars were composed of rhamnose, fructose, glucose, sucrose, maltose and an unidentified material. The content of sucrose was $92{\sim}94%$ of total free sugars. Two year old root showed the highest sugar content and it decreased since then.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.3
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• pp.280-283
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• 1985

Lipid fractions of the roots of cultured (five years old) and wild (eight years old) Codonopsis lanceolata were analyzed. The most abundant fraction of the lipids extracted from cultured and wild roots of C. lanceolata was neutral lipid and the next came phospholipid and glycolipid in descending order. The percentage, however, of the neutral lipid in total lipid was comparatively low, while that of phospholipid, particularly high; 41.30% and 29.34% in that of cultured and wild one respectively. The richest fraction of neutral lipid was triglyceride; 39.49% and 32.88% in the cultured and the wild respectively, and followed by sterol esters and free acid. Noticed amounts of sterol esters and monoglycerides which is able to be used as an emulsifiers, were contained in the neutral lipid of roots; 27.74% and 5.11% respectively. The unsaturated fatty acid fraction of the total lipid hydrolyzate contained in cultured and wild C. lanceolata roots was 72.87% and 74.37% respectively. The main fatty acid contained in the total lipid hydrolyzate was linoleic acid, and followed by linolenic acid palmitic acid. The main saturated fatty acid was palmitic acid and lauric acid.

• Korean Journal of Environmental Agriculture
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• v.16 no.1
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• pp.1-6
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• 1997

This study was carried out to identify the three antifungal substances isolated from the culture medium of Pseudomonas sp. A-183 which is antagonistic against Phytophthora capsici. The substance A and B showed positive reactions at the Molish test and Anthrone test, but negative one at the Fehling test, strongly suggesting that both substance A and B had nonreducing sugar frameworks. The substance C only exhibited the phenomenon of the UV induced fluorescence. From the qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR, the substance A and B were known to be composed to sugar and fatty acid, and showed a base peak of 171(m/e). It was identified that substance A was $(2-O-L-rhamnosyl-{\alpha}-L-rhamnosyl-{\beta}-hydroxydecanoyl-{\beta}-hydroxy$ decanoic acid) and the substance B was $({\alpha}-L-rhamnosyl-{\beta}-hydroxydecanoyl-{\beta}-hydroxy$ decanoic acid). The substance C was identified as a phenazine from the results of qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR.

• Korean Journal of Food Science and Technology
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• v.10 no.2
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• pp.119-123
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• 1978

Lipids, extracted with chloroform-methanol (2:1 by vol.) and purified, from nut and leaf of Ginkgo biloba were identified and quantitatived by column, thin layer and gas liquid chromatography. The results were summarized as follow: 1) The total content of purified lipids in the nut and leaf on the fresh weight basis were 1.32% and 2.24%, respectively. 2) The lipid fractions in the nut obtained by silicic acid colum chromatography were found to be composed of about 89% neutral lipids and about 10% compound lipids, and in the leaf were found to be composed of about 28% neutral lipids and about 72% compound lipids. 3) Among the neutral lipid fractions, triglycerides (86.2%) were the major component in the nut, but esterified sterols (53.3%) were the major component in the leaf. 4) The main fatty acids of the total lipids were oleic(37.5%) and linoleic acid(44.5%) in the nut, but linolenic(45.2%) and palmitic acid (25.1%) were main fatty acids in the leaf. The patterns of fatty acid composition of the neutral lipid fractions in the nut and leaf were found to be similar, and oleic, linoleic and palmitic acid were the predominant. A large amount of oleic and linoleic acid in the glycolipid fractions was found in the nut compared with those in the leaf, but linolenic acid content in the leaf was significantly higher than in the nut. And patterns of fatty acid composition of the phospholipid fractions in the nut and leaf were found to be similar to that of glycolipid fractions.

• Current Research on Agriculture and Life Sciences
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• v.3
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• pp.190-196
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• 1985

In order to know the effects of ginseng on the stress, histochemical and electron microsbopic observations were carried out of the adrenal cortex on the stressed rats. The experiments were carried out as follow : non-stressed after saline injection (S-NS-AD), stressed after saline injection (S-S-AD), non-stressed after ginseng injection (G-NS-AD), stressed after ginseng injection (G-S-AD). Saline and ginseng extract were treated by peritoneal injection for 30 days. The results obtained are summarized as follows : Histologically a number of small lipid droplets are found more in S-S-AD group than in S-NS-AD group, whereas in ginseng treated groups numerous large lipid droplets are found. Histochemically cholesterol and its ester, glycolipid, phospholipid and glycogen are highly found in ginseng-treated groups. On the other hand, glycogen and cholesterol are also observed in S-S-AD group. Electron microscopically a numbers of lipid droplets are observed in S-S-AD, G-NS-AD and G-S-AD group, especially large lipid droplets and moderate glycogen are found in G-NS-AD and G-S-AD group. In ginseng-treated groups and S-S-AD group, dilated SER, extended intercellular space and dense bodies are observed, and the filopodiae are found in G-S-AD group. These data suggest that ginseng was activate the metabolism and synthesis of the adrenal cortical cells on the stress.

• Applied Biological Chemistry
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• v.25 no.4
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• pp.239-247
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• 1982

Free and bound lipids of both inner and outer part in the edible portion of 3 varieties of chestnuts(Castanea crenata) were extracted and fractionated into neutral lipid, glycolipid and phospholipid, by silicic acid column chromatography. Fatty acid composition of each fraction was examined with gas chromatography. Lipid contents in chestnut on dry weight basis were 1.84% in inner part(1.26%-free lipid and 0. 58% bound lipid) and 1.95% in outer part (1.63%-free lipid and 0.32%-bound lipid). Free lipid content was $2{\sim}5$ times higher than bound lipid content and was more abundant in the outer part. Neutral lipid content of the free lipid in the inner part was 39.5%, approximately 10 times as much as that of the bound lipid, but in the outer part, the former was 56.6%. Glycolipid content was 22. 0% in the free lipid and 17.0% in the bound lipid. Phospholipid content was 6.9% in the free lipid and 10.5% in the bound lipid. The predominant fatty acids were linoleic acid, palmitic acid and linolenic acid in the free lipid, and palmitic acid, linoleic arid and linolenic acid in the bound lipid, Therefore, saturated fatty acid content in the bound lipid was 2 times higher than in the free lipid.

• Korean Journal of Agricultural Science
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• v.18 no.2
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• pp.119-126
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• 1991

The lipid fractions, lipid classes and free fatty acids in total lipids from cheese and soybeans were analyzed by column, thin-layer and gas chromatographies. The percentages of neutral lipid, glycolipid and phospholipid in cheese were 96.2, 1.1 and 0.7, whereas those in soybeans were 87.5, 0.5 and 4.3. Major lipid classes of total lipid, and neutral lipid were triglyceride, fatty acid, cholesterol, diglyceride, monoglyceride and polar lipid, and those of glycolipid and phospholipid were triglyceride, diglyceride, monoglyceride and polar lipid in total lipid from cheese. Large amounts of triglyceride and polar lipid and small amounts of diglyceride, monoglyceride and polar lipid were detected in all lipid fractions from soybeans. The higher proportion of C4:0, C6:0, C8:0, C10:0, C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 and C18:2 fatty acids were found in total lipid from cheese, whereas those of C18:0, C18:2 and C18:3 fatty acids were found in total lipid from soybeans. Most predominant fatty acids are C16:0 for the total lipid of cheese and C18:2 for the total lipid of soybeans. The lower proportions of C14:1, C15:0, C17:0 and C20:0 fatty acids in total lipid from cheese and C4:0, C6:0, C10:0 and C18:0 in total lipid from soybeans were detected.

• Korean Journal of Environmental Biology
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• v.18 no.4
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• pp.395-401
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• 2000

Growth and biochemical composition were analyzed in Gymnodinium sanguineum and Skeletonema costatum grown in media containing various nitrate and phosphate concentrations. Concentrations of nitrate and phosphate in the growth media were 0, 0.3, 0.6, 0.9, 1.2mM and 0, 15, 30, 45, 60 $\mu$M, respectively. Growth of G. sanguineum was suppressed in the low concentration of nitrate and phosphate (below N = 0.3 mM and P = 15 $\mu$M), whereas growth of S. costatum did not changed. At the low concentrations of nitrate and phosphate, amount of intracellular protein and carbohydrate in G. sanguineum cells were largely decreased, whereas content of carbohydrate in S. costatum cells was increased little. Amount of neutral lipid and phospholipid in G. sanguineum didn't changed, but concentration of glycolipid was largely decreased in the medium containing low concentrations of nitrate and phosphate. However the levels of TAG, glycolipid, and phospholipid did not changed in S. costatum cells. These results show that S. costatum is more adaptable than G. sanguineum in the low concentration of nitrate and phosphate.