Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Comparison on Growth and Biochemical Composition of Gymnodinium sanguineum and Skeletonema costatum Grown in Different N, P Concentrations

질산염과 인산염 농도 변화에 따른 Gymnodinium sanguineum과 Skeletonema costatum의 성장과 생화학적 구성성분의 비교

  • Lee, Taek-Kyun (Harmful Algal Research Unit, Korean Ocean Research and Development Institute) ;
  • Park, Myung-Whan (Environmental Bioresources Laboratory, Research Institute of Bioscience and Biotechnology) ;
  • Shin, Kyoung-Soon (Harmful Algal Research Unit, Korean Ocean Research and Development Institute) ;
  • Chang, Man (Harmful Algal Research Unit, Korean Ocean Research and Development Institute)
  • 이택견 (한국해양연구소 해양환경기후본부 유독식물플랑크톤 연구사업단) ;
  • 박명환 (생명공학연구소 환경생물소재연구실) ;
  • 신경순 (한국해양연구소 해양환경기후본부 유독식물플랑크톤 연구사업단) ;
  • 장만 (한국해양연구소 해양환경기후본부 유독식물플랑크톤 연구사업단)
  • Published : 2000.12.01
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Abstract

Growth and biochemical composition were analyzed in Gymnodinium sanguineum and Skeletonema costatum grown in media containing various nitrate and phosphate concentrations. Concentrations of nitrate and phosphate in the growth media were 0, 0.3, 0.6, 0.9, 1.2mM and 0, 15, 30, 45, 60 $\mu$M, respectively. Growth of G. sanguineum was suppressed in the low concentration of nitrate and phosphate (below N = 0.3 mM and P = 15 $\mu$M), whereas growth of S. costatum did not changed. At the low concentrations of nitrate and phosphate, amount of intracellular protein and carbohydrate in G. sanguineum cells were largely decreased, whereas content of carbohydrate in S. costatum cells was increased little. Amount of neutral lipid and phospholipid in G. sanguineum didn't changed, but concentration of glycolipid was largely decreased in the medium containing low concentrations of nitrate and phosphate. However the levels of TAG, glycolipid, and phospholipid did not changed in S. costatum cells. These results show that S. costatum is more adaptable than G. sanguineum in the low concentration of nitrate and phosphate.

질산염과 인산염의 농도가 다른 배지에서 Gymnodinium sanguineum과 Sketetonema costotum의 성장과 세포구성성분을 비교 분석하였다. 질산염은 0, 0.3, 0.6, 0.9, 1.2 mM, 인산염은 0, 15, 30, 45, 60$\mu$M을 각각 첨가한 배지에서 배양하였을 때, 저농도의 질산염 및 인산염 배지에서 G. sanguineum의 성장은 억제되었으나, S. costatum은 성장에 영향을 받지 않은 것으로 나타났다. 저농도의 질산염과 인산염 배지에서 G. sanguineum의 세포내 단백질 및 탄수화물의 양은 감소하였으나, S. costutum은 탄수화물의 양이 조금 증가하는 경향을 보였다. 세포내 지질은 G. sunguineum의 경우 중성지질과 인지질은 변화가 거의 없는 반면, 당지질은 저농도의 질산염 및 인산염에서 크게 감소하였다. 그러나 S. costatum 세포내의 세 종류의 지질의 양은 변화를 보이지 않았다. 이와 같은 결과는 저농도의 질산염 및 인산염 배지에서 와편모조류인 G. sanguineum보다 규조류인 S. costatum이 더 잘 적응할 수 있다는 것을 보여준다.

Keywords

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