• 한국작물학회지
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• 제23권1호
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• pp.86-89
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• 1978

중과 일년생잡초와의 경합시기와 잡초경합에 의한 콩의 수량감소를 추정하기 위하여 파종후 2주간격으로 10주까지 그리고 전생육기간동안 잡초를 제거하지 않고 그 이후 제초하였을 때 콩의 생육 및 수량에 미치는 영향을 조사하였으며 그 결과를 요약하면 다음과 같다. 1. 본실험포장의 우점잡초는 피, 바랭이, 참방동산이, 여뀌, 쇠비름, 쑥, 명아주 등이었다. 2. 잡초와의 경합기간이 길어질수록 콩의 생장이 억제되어 콩의 LAI와 CGR이 크게 감소되었다. 3. 초장, 주당분지수 및 주당협수도 잡초와의 경합기간이 길어짐에 따라 감소하는 경향이었다. 4. 콩의 수량은 파종후4주 이전까지 잡초를 제거하기 시작한 경우 잡초외 경합에 의하여 3∼6％ 감소되었으나 8주이후에 서초를 하면 수양감소가 심하였다. 5. 중의 수량과 초장을 제외한 LAI. CGR. 주당협수 및 분지수간에는 유의상관을 나타내었다.

• Journal of Plant Biology
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• 제17권4호
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• pp.163-170
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• 1974

Leaf tissue of Glycine max Merr. was fixed in para-formaldehyde-glutarldehyde and postfixed in osmium tetroxide or postassium permanganate for electron microscopy. The origin of cytolysome-like organelles of mesophyll cell was studied and changes of fine structure of the organelles according to treating solutions such as gibberellin (GA), kinethin (KI), 2,4-dichlorophenoxy acetic acid(2, 4-D) or 2, 4-D＋GA(1mg/l, respectively) were observed. The cylolysome-like organelles differentiate in endoplasmic reticulum and plasmalemma, and they drop into vacuoles being isolated from the formers. They seem to change into myelin-like structure and to be degenerated by autodigestion. Cytolysome-like organelles involved in cell walls and vacuoles showed activity of acid phosphatase. In the group of GA and KI treatment, cytolysome-like organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D＋GA, myelin-like structures increased in size and autodigestion of this organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D＋GA, myelin-like structures increased in size and autodigestion of this organelle seemed to be accelerated. In the treatmental group of 2,4-D＋GA, myelin-like structures shown high electron density were observed in cytoplasm and vacuoles together.

• The Korean Journal of Ecology
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• 제10권4호
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• pp.175-182
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• 1987

Soybeans(Glycine max Merr. cv. Kwanggyo), inoculated with Rhizobium japonicum 110 and then sand-cultured with nitrate gradients (0, 1, 3, 10 and 30mM KNO3). were studied on the growth analysis, nitrogen fixation and nitrogen economy during the growing period. The maxium values of total leaf area, biomass and nitrogen quantity were increased 139%, 122% and 161%, respectively with higher concentration of nitrate treatment. Nodulation showed significant linear correlation with leaf area growth for each treatment of nitrate concentration increased. The more nitrate concentration increased, the more distribution ratios of dry matter and nitrogen to nodule decreased, and the more T/R ratios, CGR and N content increased. On the other hand, F/C ratios and RGR showed little changes. The amounts of nitrogen fixation of soybean alloted to 0, 1, 3, 10 and 30mM nitrate treatments were 100, 46, 14, 0.1 and 0.004% for the total nitrogen assimilation, respectively. The nitrogen utility of soybean plant was smaller than that of other plants and ranged from 23 to 30 at varying nitrate gradients.

• Plant Resources
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• 제1권2호
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• pp.81-91
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• 1998

Fifty-nine Korean soybean (Glycine max L. Merr.) landrace accessions were tested for genotype fingerprinting, differentiation and association between morphological traits and SSR profile. Using 8 SSR loci, 59 varieties were divided into 55 groups, and only 4 pairs of varieties were not uniquely identified. The resolving power of SSR for soybean genotyping was much higher than that of the morphological traits that were studied. Identification efficiency also differed among SSR loci. Those loci with higher numbers of alleles distinguished varieties more effectively. Genetic differentiation values of the soybean landraces varied from 0.57 to 0.82 with a mean of 0.68. The number of alleles detected by the 8 loci ranged from 3 to 8. and the effective number of alleles ranged from 2.3 to 5.1. In a study of the association of SSR alleles with morphological traits, some alleles seemed to be related with some specific morphological traits. Comparison of two kinds of dendrograms which were derived from SSR markers and quantitative traits indicated that the dendrograms were not consistent. Considering the correlation between single SSR locus and qualitative traits governed by major genes, the data suggest that alleles of microsatellite loci be more closely related to some traits determined by major genes than those determined by minor genes.

• 한국자원식물학회지
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• 제24권3호
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• pp.319-323
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• 2011

The purpose of this study is to analyze antioxidant activity and multidrug resistance reversing activity in several Korean colored soybean (Glycine max Merr.) cultivars. Antioxidant activity of methanol extracts from colored soybean cultivars was evaluated by TBARS (thiobarbituric acid reactive substances), DPPH (1, 1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) methods. By means of TBARS, cultivar "Jeonnam #1" showed the highest activity until 7 days, and followed by "Black #1", "Jinyul" and "Black #3", showing lower activity than that of BHT (butylated hydroxytoluene). Methanol extracts of all cultivars proved that DPPH radical scavenging activity is dose-dependent. Methanol extract from cultivar "Jeonnam #1" showed highest DPPH radical scavenging activity, and followed by cultivars "Black #1". MDR (multi-drug resistance reversing) activity, however, showed the highest effect in "Black #3" and the lowest "Black #1" cultivar. These results suggest that seed colors of soybean may play an important role in antioxidant activity and MDR activity.

• 한국작물학회지
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• 제49권4호
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• pp.342-348
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• 2004

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the $60\%$ isopropanol extract of soybean(Glycine max [L.] Merr.) seed revealed two abundant proteins with molecular masses of 19 and 10 kDa. Amino acid analysis revealed that the isopropanol-extractable protein fraction was rich in cysteine. Two-dimensional gel electro-phoretic analysis indicated that the 19kDa and 10kDa proteins had pI of 4.2 and 4.0 respectively. Peptide mass fingerprints of trypsin digests of the two proteins obtained using matrix-assisted, laser desorption/ionization-time of flight (MALDI-TOF) mass spectroscopy revealed the 19kDa protein was Kunitz trypsin inhibitor and the 10kDa protein was Bowman-Birk proteinase inhibitor. When resolved under non-denaturing conditions, the isopropanol-extracted proteins inhibited trypsin and chymotrypsin activity. Results presented in this study demonstrate that isopropanol extraction of soybean seed could be used as a simple and rapid method to obtain a protein fraction enriched in Kunitz trypsin and Bowman-Birk proteinase inhibitors. Since proteinase inhibitors are rich in sulfur amino acids and are putative anticarcinogens, this rapid and inexpensive isolation procedure could facilitate efforts in nutrition and cancer research.

• 한국작물학회지
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• 제48권2호
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• pp.81-88
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• 2003

Soybeans [Glycine max (L.) Merr.] are frequently exposed to unfavorable environments during growing seasons and water is the most important factor limiting for the production system. The purpose of this study was to determine the leaf water potential changes by irrigation, and to evaluate the relationships of leaf water potential, growth and yield in soybeans. Three soybean cultivars, Hwangkeumkong, Shinpaldalkong 2, and Pungsannamulkong, were planted in growth chamber and field with irrigated treatments. Leaf water potential of three soybean cultivars was positively correlated with leaf water content during vegetative and reproductive growth stages in growth chamber and field experiments. Leaf water potentials measured for three soybean cultivars under growth chamber were higher than those of under field conditions. Higher leaf water potential with irrigated plots under field was observed compared to conventional plots during reproductive growth stages. Leaf water potentials of three soybean cultivars were continually decreased during reproductive growth stages under field and there was no significant difference among them. Number of leaves, leaf water content, pod dry weight, number of seeds and seed dry weight with irrigated plots were higher than those of conventional plots. The results of this study suggested that leaf water potential could be used as an important growth indicator during the growing season of soybean plants.

• 동의생리병리학회지
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• 제31권6호
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• pp.380-384
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• 2017

From March 1, 2017 to May 31, 2017, subjects were allowed to apply a wrinkle-improving herbal cosmetic product containing the extract of Douchi for 8 weeks. The effectiveness of herbal cosmetics was evaluated through wrinkle improvement efficacy parameter analysis, subjective wrinkle improvement through questionnaires, and adverse reaction. The herbal cosmetic products containing the extract of Douchi showed a significant decrease in the wrinkle improvement efficacy parameter analysis result. The results of the subjective satisfaction analysis after use were also evaluated positively. We could confirm the possibility of wrinkle improvement effect of Douchi extract, and it will be possible to contribute to the expansion of korean medicine field through more studies in the future.

• 한국작물학회지
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• 제45권1호
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• pp.1-5
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• 2000

Leaf area is critical for crop light interception, and thereby has a substantial influence on crop yield. This experiment was conducted to characterize the development of soybean [Glycine max (L.) Merr.] leaf area. Plastochron index and leaf relative growth rate of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. First, plastochron ratio (PR) and plastochron index (PI) were evaluated in greenhouse to compare the leaf growth rate between two genotypes under well-watered condition. There was reasonable constancy of PR between two genotypes. The PR means of Jackson and PI416937 were 0.41 and 0.44, respectively. A fairly smooth increase of PI during vegetative stage was observed. Second, the relative growth rates were graphed against leaf area, normalized with respect to final leaf area, under well-watered and water-deficit conditions. Leaf growth was sustained longer in well-watered condition than water-deficit condition and there was a sizable proportion of leaves which was ceased earlier their growth in water-deficit condition compared to well-watered condition. The leaf relative growth rate of Jackson until leaves had completed at 45% of their growth during water deficit period was higher than that of PI416937.

• 한국작물학회지
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• 제45권1호
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• pp.6-13
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• 2000

Water deficit is the primary constraint of soybean [Glycine max (L.) Merr.] yield, and a physiological understanding of processes affected by water deficit is a key step in identifying and improving drought tolerance in soybean. The objectives of this research were to evaluate biomass and nitrogen accumulation patterns and water use efficiency (WUE) as possible mechanisms associated with the drought tolerance of Jackson. Biomass accumulation of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. For water-deficit treatment, total biomass accumulation was negligible for PI416937, but biomass accumulation continued at approximately 64 % of the well-watered treatment of Jackson. Transpirational losses for Jackson and PI416937 were approximately the same for the water-deficit treatment, indicating that Jackson had superior WUE. Isotopic discrimination of $^{13}$ C relative to $^{12}$ C also indicated that Jackson had higher WUE. Results indicated that increased WUE for Jackson under water deficit showed it was tolerant to drought rather than had an avoidance mechanism.