• 한국염색가공학회지
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• 제20권5호
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• pp.1-6
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• 2008

3-(Hydroxyphenyl phosphinyl) propanoic acid (HPPA) has been one of the most commonly used durable flame retardant agents for polyethylene terephthalate (PET) for many years. We intended to explore the application of HPPA to cellulose fabrics as formaldehyde-free phosphorus based flame retardants (FRs) through green chemistry process. The flame retardancy of the flame-retardant treated cellulose fabrics were characterized by using inductively coupled plasma spectroscopy (ICP) and limiting oxygen index (LOI). Structural changes of the treated cellulose fabrics were carried out by thermogravimetric analysis (TGA) and Fourier transform infrared (FT-IR) spectroscopy. To enhance the flame retardancy of HPPA treated cellulose fibers, glycerol polyglycidyl ether (GPE), a crosslinking agent was employed. Both HPPA and GPE treated cotton fabric imparted an LOI value over 26.

• 한국응용과학기술학회지
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• 제23권2호
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• pp.125-129
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• 2006

Benzoxazolo carbocyanine compounds were synthesized by condensation of a suitable ortho-ester with an appropriately substituted 2-methylbenzoxazole in the presence of triethylamine. This compounds used as green sensitizing dyes in photographic emulsions. The compounds are characterized by fast atom bombardment mass spectrometry. The values(m/z) of structurally significant ions observed in FAB spectra. It was showed tentative fragmentation pattern in FAB spectroscopy of $HN(C_2H_5)_3$ cations in glycerol/trifluoroacetic acid matrix.

• 한국동물학회지
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• 제37권4호
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• pp.524-530
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• 1994

An Arpase complex has been purified to apparent homogeniety from the extract of chick skeletal muscle using conventional column chromatographies and glycerol density gradient centrifugation. This eWe has a sedimentation coefficient of 15S as determined by the gradient centrifugation and therefore is referred to as the 15S ATPase. It behaves as a 600-kOa molecule upon gel filtration analysis using a Superose-6 column. However, the ATPase runs as a 95-kDa polvpeptide when analyzed by polvacrvlamide gel electrophoresis in the presence of sodium dodecyl sulfate. Thus, the Arpase is likely to consist of six identical subunits of 95 KDa. It has a Km value of 0.6 mM for ATP and is maximally active at pH 9.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2003년도 제23차 추계총회 및 국제학술심포지움
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• pp.164.2-165
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• 2003

Structured lipids (SLs) are defined as triacylglycerols to change the fatty acid composition in the glycerol backbone and lipases are known as a powerful tool for the syntheses of SLs. Structured lipid from corn oil, capric acid, and conjugated linoleic acid (CLA) by transesterification reaction and using several amounts of immobilized lipase RM IM (from Rhizomucor miehei) was studied, and 4% of lipase amount was selected for further study as the optimal amount. Comparison the chemical properties (free fatty acid value, iodine value, saponification value, tocopherols, and color analysis), solidification behavior, and volatile fractions (from headspace SPME GC-MS) between com oil and SL com oil was obtained.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회B
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• pp.2460-2464
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• 2008

The present paper suggests new method to know the effects of molecular diffusion and the helicity of microchannel flows on mixing in passive micromixers, which are essential components of a microfluidic chip. In this study, 'Helix Index' is newly defined as the magnitude of chaotic advection. Relationship between Helix Index and Mixing Index is analyzed numerically such as the wide range of Peclet and Reynolds numbers in three dimensional serpentine microchannel when using soluble solutions (water/glycerol). As a result, a simple algebraic equation is derived by this relationship based on a regression analysis. The algebraic equation is found to be able to accurately predict the mixing performance without solving the coupled, complex momentum and mass transfer equations.

• Journal of Microbiology and Biotechnology
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• 제29권10호
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• pp.1591-1602
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• 2019

To shed light on the genetic basis of salt tolerance in Enterococcus faecium, Enterococcus faecalis, and Tetragenococcus halophilus, we performed comparative genome analysis of 10 E. faecalis, 11 E. faecium, and three T. halophilus strains. Factors involved in salt tolerance that could be used to distinguish the species were identified. Overall, T. halophilus contained a greater number of potassium transport and osmoprotectant synthesis genes compared with the other two species. In particular, our findings suggested that T. halophilus may be the only one among the three species capable of synthesizing glycine betaine from choline, cardiolipin from glycerol and proline from citrate. These molecules are well-known osmoprotectants; thus, we propose that these genes confer the salt tolerance of T. halophilus.

• 생명과학회지
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• 제28권7호
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• pp.786-794
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• 2018

누에(Bombyx mori)번데기는 창상치료용 드레싱, 간보호활성, 항유전독성, 포도당농도 조절, 항암활성 등에 다양하게 적용되고 있다. 본 연구에서는 붉은동충하초(Cordyceps militaris)를 이용하여 발효시킨 누에번데기분말의 에탄올 추출물(Ethanol extract of silkworm pupae fermented with Cordyceps militaris, ESfC)의 항비만효능을 분석하기 위하여, SD랫드로부터 분리된 일차지방세포(Primary adipocytes)에서 농도별로 ESfC를 처리하여 free glycerol release, cAMP농도를 측정하였고, 고지방사료(High fat diet, HFD)가 처리된 C57BL/6N 비만마우스에 ESfC를 투여하여 지방조직과 간조직에서 지방축적(Fat accumulation)을 관찰하였다. 먼저, 지방감소효과를 나타내는 지표물질로서 cordycepin의 존재는 HPLC분석을 통하여 ESfC에서 확인하였다. 또한, free glycerol수준과 cAMP농도는 고농도 ESfC가 처리된 일차지방 세포에서 유의적으로 증가하였지만 이들 수준은 저농도와 중농도의 ESfC로처리세포에서는 유의적인 변화가 없었다. HFD-유도 비만모델에서, 고지방사료의 투여에 의해 유의적으로 증가된 지방무게와 지방세포의 크기는 ESfC처리그룹에서 감소되었다. 간 무게와 지방방울(Lipid droplet)의 수는 고지방사료의 투여에 의해 증가하였지만 ESfC투여에 의해 유의적으로 감소하였다. 또한, 유사한 회복효과는 perilipin과 HSL의 인산화 수준뿐만 아니라 ATGL의 발현수준에서도 관찰되었다. 따라서 이상의 결과는 ESfC가 지방일차세포에서 지방분해를 촉진하고, HFS유도 비만모델에서 지방 축적을 억제하는 효과를 나타냄을 보여주고 있으며, 향후 비만환자에 처리하기 위한 약물로서 사용가능성을 제시하고 있다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제12권2호
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• pp.20-26
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• 2007

매립지 침출수로 오염된 지하수의 미생물 군집특성을 31가지 탄소원을 가지는 Ecoplate를 사용하여 조사하였다. 각 기질에 대한 탄소이용도는 시료에 따라 달랐고, 2-hydroxy benzoic acid, D,L-$\alpha$-glycerol phosphate, D-malic acid는 모든 시료에서 기질로써 이용되지 못했고, D-xylose, D-galacturonic acid, L-aspargine, tween 80, L-serine는 모든 시료에서 탄소원으로 사용되었고, 나머지는 시료에 따른 차이를 보였다. AWCD에 의한 통계분석결과는 KSG1-16 > KSG1-12 > KSG1-07 > KSG-08 > KSG1-13 순인데 예상대로 비오염 지하수 시료인 KSG1-13가 가장 낮았으며, 오염 된 지하수 시료 KSG-07과 KSG1-08는 이보다 높았으나 처리수인 KSG1-16이 높은 것은 의외이었다. PCA방법에 의한 통계 분석결과는 침출수(KSG1-12) 및 오염지하수(KSG1-07, KSG1-08)와 오염되지 않은 처리수(KSG1-16) 및 비오염 지하수(KSG1-13)로 크게 둘로 유사성이 구분되었다. 그러나, 미생물의 다양성을 나타내는 Shannon index 값에 의한 분석은 시료간의 별 차이를 나타내지 않아 서로 유사하였다.

• BMB Reports
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• 제37권5호
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• pp.556-564
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• 2004

A recombinant Fab monoclonal antibody (Fab) C37, previously obtained by phage display and biopanning of a random antibody fragment library against Burkholderia pseudomallei protease, was expressed in different strains of Escherichia coli. E. coli strain HB2151 was deemed a more suitable host for Fab expression than other E. coli strains when grown in media supplemented with 0.2% glycerol. The expressed Fab fragment was purified by affinity chromatography on a Protein G-Sepharose column, and the specificity of the recombinant Fab C37 towards B. pseudomallei protease was proven by Western blotting, enzyme-linked immunosorbent assay (ELISA) and by proteolytic activity neutralization. In addition, polyclonal antibodies against B. pseudomallei protease were produced in rabbits immunized with the protease. These were isolated from high titer serum by affinity chromatography on recombinant-Protein A-Sepharose. Purified polyclonal antibody specificity towards B. pseudomallei protease was proven by Western blotting and ELISA.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.244-252
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• 2007

Escherichia coli has a PhoR-PhoB two-component regulatory system to detect and respond to the changes of environmental phosphate concentration. For the E. coli W3110 strain growing under phosphate-limiting condition, the changes of global gene expression levels were investigated by using DNA microarray analysis. The expression levels of some genes that are involved in phosphate metabolism were increased as phosphate became limited, whereas those of the genes involved in ribosomal protein or amino acid metabolism were decreased, owing to the stationary phase response. The upregulated genes could be divided into temporarily and permanently inducible genes by phosphate starvation. At the peak point showing the highest expression levels of the phoB and phoR genes under phosphate-limiting condition, the phoB- and/or phoR-dependent regulatory mechanisms were investigated in detail by comparing the gene expression levels among the wild-type and phoB and/or phoR mutant strains. Overall, the phoB mutation was epistatic over the phoR mutation. It was found that PhoBR and PhoB were responsible for the upregulation of the phosphonate or glycerol phosphate metabolism and high-affinity phosphate transport system, respectively. These results show the complex regulation by the PhoR-PhoB two-component regulatory system in E. coli.