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Characterization of Microbial Communities in a Groundwater Contaminated with Landfill Leachate using a Carbon Substrate Utilization Assay  

Koo, So-Yeon (Department of Environmental Science and Engineering, Ewha Womans University)
Kim, Ji-Young (Department of Environmental Science and Engineering, Ewha Womans University)
Kim, Jai-Soo (Department of Life Science, Kyonggi University)
Go, Kyung-Seok (Korea Institute of Geoscience & Mineral Resources)
Lee, Sang-Don (Department of Environmental Science and Engineering, Ewha Womans University)
Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
Go, Dong-Chan (Korea Institute of Geoscience & Mineral Resources)
Publication Information
Journal of Soil and Groundwater Environment / v.12, no.2, 2007 , pp. 20-26 More about this Journal
Abstract
The microbial community properties of groundwater samples contaminated with landfill leachates were examined using Ecoplate including 31 sole carbon sources. The samples were KSG1-12 (leachate), KSG1-16 (treated leachate), KSG1-07 (contaminated groundwater), KSG1-08 (contaminated groundwater), and KSG1-13 (uncontaminated groundwater). Among the carbon sources used as substrates, 2-hydroxy benzoic acid, D,L-$\alpha$-glycerol phosphate, and D-malic acid were not utilized in any sample, while D-xylose, D-galacturonic acid, L-aspargine, tween 80, and L-serine were utilized in all 5 samples. The rest of substrates showed very different patterns among the samples. Average well color development (AWCD) analysis demonstrated that the potential activity on 31 substrates was in the order of KSG1-16 > KSG1-12 > KSG1-07 > KSG-08 > KSG1-13, which generally agrees with the degree of pollution, except KSG1-16. Principal component analysis (PCA) on similarity between samples showed two groups (KSG1-12, -07 and -08 vs KSG1-16 and -13), coinciding with contaminated and uncontaminated groups. Shannon index showed that the microbial diversities were similar among the samples.
Keywords
Microbial communities; Carbon source utilization; Landfill leachate; Groundwater; Ecoplate;
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