• Korean Journal of Exercise Nutrition
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• v.14 no.2
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• pp.75-80
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• 2010

We evaluated the effect of the ginsenoside Re on insulin resistance of glucose transport in muscles of rats made insulin resistant with a high fat diet. After a week of adaptation period to the laboratory environment, 40 male wistar rats were randomly assigned into 2 groups (Chow diet group; CD, n = 20, High fat diet group; HFD, n = 20). After 5-week of high fat diet, Food was removed after 6:00 PM the day before the experiment. The following morning, rats were anesthetized by an intraperitoneal injection of pentobarbital sodium (50 mg/kg body wt), and the soleus muscles were removed. Before incubation, the soleus muscle was split longitudinally into strips with an average weight of 15~20 mg. After the muscle dissection was completed, the abdominal cavity was opened, and the epididymal, mesenteric, and retroperitoneal fat pads were removed and weighed. Treatment of muscles with ginsenoside Re alone had no effect on glucose transport. The high fat diet resulted in ~50% decreases glucose transport rate in soleus muscles. Treatment of muscles with ginsenoside Re in vitro for 90 min completely reversed the high fat diet-induced insulin resistance of glucose transport in soleus muscles. This effect of ginsenoside Re is specific for insulin stimulated glucose transport, as Re treatment did not reverse the high fat diet-induced resistance of skeletal muscle glucose transport to stimulation by contraction. Our results show that the ginsenoside Re induces a remarkably rapid reversal of high fat diet-induced insulin resistance of muscle glucose transport.

• The Journal of Internal Korean Medicine
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• v.22 no.3
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• pp.397-403
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• 2001

Objectives; This study was carried out to investigate the motor activity, glucose transport and metabolism of Jiaweizhengqi-tang(JKT) in rat small intestine. Methods ; The motor activity of the rat small intestine has been investigated by means of measuring barium sulfate passage degrees. Transport and metabolism of glucose were studied in everted sac of rat small intestine with incubation under several conditions. Results; Atropine treatment significantly delayed barium sulfate transit, and JKT pretreatment increased intestinal motor activity, but not significant. JKT administration showed renal toxicity in animal experiment, so clinical safety should settled to use commonly. The transport and metabolism of glucose were greater at jejunum than ileum. So, everted jejunum of rat were used to study the effect of JKT. When JKT were treated, the concentration of glucose were higher than untreated group. This result was thought to be influenced by the glucose in JKT. When 2, 4 dinitrophenol was treated, the transport and metabolism of glucose were decreased, but JKT treated together, the concentration of glucose in serosal solution increased. Conclusions; The transport and metabolism of glucose were influenced by the glucose in JKT. And the effects of JKT were still unidentified, but through continuous investigation, these effects of JKT should be identified.

• The Journal of the Korea Contents Association
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• v.9 no.4
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• pp.340-346
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• 2009

Lithium has only a minimal effect on basal glucose transport activity, instead that lithium markedly increased the sensitivity of glucose transport to insulin by increasing in insulin induced glucose transport activity. And Lithium increases in insulin responsiveness as well. Previous studies has reported this enhancement of lithium to stimulate the glucose transport process is not only limited to insulin, it also induce the increases in the sensitivity of glucose transport by submaximal contractile activity. The preliminary study, however, leads that Lithium possibly improves the responsiveness of glucose transport with maximal muscle contraction. In this study, we investigated the effect of Lithium on contraction for the maximal glucose transport. For the purpose of this study, Epitrochlearis muscles of SD rat were isolated and treated Lithium with electric contraction and/or insulin to activate the maximal glucose transport. The results support that Lithium improves the responsiveness of glucose transport through potentiates contraction and/or insulin induced-glucose uptake in muscle. Consequently Lithium treated with muscle contraction and insulin has the important potential to improve the insulin resistance and diabetes.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.5
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• pp.1330-1336
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• 2005

This study was carried out to investigate the motor activity and glucose transport and metabolism of Gaewool-Whadam-Jian(GWJ) in rat gastro-intestinal tract. The motor activity of the rat gastro-intestinal tract has been investigated by means of measuring barium sulfate passage degrees. Atropine treatment significantly delayed barium sulfate transit, and GWJ pretreatment increased intestinal motor activity, but not significant. GWJ administration showed no toxicity to kidney and liver. Transport and metabolism of glucose were studied in everted sac of rat small intestine with incubation under several conditions. The transport and metabolism of glucose were greater at jejunum than ileum. So, everted jejunum of rat were used to study the effect of GWJ. When GWJ were treated, the concentration of glucose were higher than untreated group. This result was thought to be influenced by the glucose in GWJ. When 2, 4 dinitrophenol and phlorizin were treated, the transport and metabolism of glucose were decreased, but GWJ treated together, the concentration of glucose in serosal solution increased. Gastric juice secretion and total acidity significantly decreased by administration of GWJ through duodenum region. The mechanism of effect of GWJ was still unidentified, Dut through continuous investigation, the effect of GWJ should be investigated.

• Journal of Nutrition and Health
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• v.30 no.2
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• pp.210-219
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• 1997

Retarding effects of the dietary fibers from tangerine peels on glucose, bile acid and cadmium transport were evaluated by dialysis method, and were compared with those of commercial dietary fibers(citrus pection, CM-cellulose, guar gum, $\alpha$-cellulose). Yields of total (TDF), insoluble(IDF) and soluble dietary fibers(SDF) from tangerine peels on the fresh matter basis were 2.84%, 1.95% and 0.39% respectively. The amount of insoluble fibers was 5.2 times higher than that of soluble fibers. Soluble fibers(guar gum, CM-cellulose, SDF, pectin) had the retarding effect on glucose transport, while IDF, TDF and $\alpha$-cellulose did not have. Guar gum showed the greatest effect, followed by CM-cellulose, SDF and pectin. Among the extracted fibers, only SDF had the effect on glucose transport retardation. Regarding bile acid dialysis, guar gum had the greatest retarding effect, and all dietary fibers from tangerine peels, especially SDF, showed the effect of bile acid retardation. On cadmium transport retardation, CM-cellulose had the greatest effect, followed by SDF, TDF, IDF, guar gum and pectin. Among the extracted fibers, SDF had the greatest effect on Cd trasport transport retardation. The extracted dietary fibers showed higher retarding effect on Cd transport than glucose and bile acid transport, and the effect of SDF was higher than IDF.

• Diabetes and Metabolism Journal
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• v.42 no.6
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• pp.465-471
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• 2018

My professional journey to understand the glucose homeostasis began in the 1990s, starting from cloning of the promoter region of glucose transporter type 2 (GLUT2) gene that led us to establish research foundation of my group. When I was a graduate student, I simply thought that hyperglycemia, a typical clinical manifestation of type 2 diabetes mellitus (T2DM), could be caused by a defect in the glucose transport system in the body. Thus, if a molecular mechanism controlling glucose transport system could be understood, treatment of T2DM could be possible. In the early 70s, hyperglycemia was thought to develop primarily due to a defect in the muscle and adipose tissue; thus, muscle/adipose tissue type glucose transporter (GLUT4) became a major research interest in the diabetology. However, glucose utilization occurs not only in muscle/adipose tissue but also in liver and brain. Thus, I was interested in the hepatic glucose transport system, where glucose storage and release are the most actively occurring.

• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.99-102
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• 1999

Glucose - induced arginine transport system was induced by a exogenous application of NH$_4$^+ ion. The uptake rate of arginine (Arg) depended on the external NH$_4$^+ ion concentration. The uptake rate was inhibited by the presence of NH$_4$^+ ion within 1 min, whereas it increased maximally after 30 min. Glucose and NH$_4$^+ ion induced the same arginine transport system. Km value of Arg transport systems was 2 $\mu$M, and V_(max) was 60 $\mu$mol^(-1) . h . g fresh weight^(-1) for NH$_4$^+ ion and 174 $\mu$mol^(-1) . h . g fresh weight^(-1) for glucose induced transport system. But, the transport system of Glu for glucose and NH$_4$^(-1) ion induced had different Km values. Km value of Glu was 285 $\mu$M for glucose - and 58 $\mu$M for NH$_4$^+ ion induced transport system. Thus, NH$_4$^+ ions play a important role as inducer for the glutamine transport system. NH$_4$^+ ion induced glutamine system was inhibited over 90% by cycloheximide. We concluded that a new carrier protein for glutamine was induced by NH$_4$^+ ion.

• Journal of fish pathology
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• v.14 no.1
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• pp.54-58
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• 2001

The seasonal trends of plasma protein and glucose concentrations in marbled soles(Limanda yokohamae) and greenlings(Hexagrammos otakii), and the influence of transport stressor on those levels were investigated. Total plasma protein levels of marbled soles and greenlings in late spring and summer were significantly higher than those in winter(January). Plasma glucose levels were consistently increased according to elevation of water temperature both in marbled soles and greenlings. Transport stressor gave rise to decrease of plasma protein levels and increase of blood glucose levels.

• Biomedical Science Letters
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• v.15 no.1
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• pp.55-60
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• 2009

Insect cells such as Spodoptera frugiperda Clone 9 (Sf9) cells are widely chosen as the host for heterologous expression of a mammalian sugar transport protein using the baculovirus expression system. Characterization of the expressed protein is expected to include assay of its function, including its ability to transport sugars and to bind inhibitory ligands such as cytochalasin B. It is therefore very important first to establish the transport characteristics and other properties of the endogenous sugar transport proteins of the host insect cells. However, very little is known of the transport characteristics of Sf9 cells, although their ability to grow on TC-100 medium strongly suggested the presence of endogenous glucose transport system. In order to investigate the substrate and inhibitor recognition properties of the Sf9 cell transporter, the ability of pentoses to inhibit 2-deoxy-D-glucose (2dGlc) transport was investigated by measuring inhibition constants $(K_i)$. To determine the time period over which of sugar into the Sf cells was linear, the uptake of 2dGlc 0.1mM extracellular concentration was measured over periods ranging from 30 seconds to 30 minutes. The uptake was linear for at least 2 minutes at the concentration, implying that uptake made over a 1 minute time course would reflect initial rates of the sugar uptake. The data have also revealed the existence of a saturable transport system for pentose uptake by the insect cells. The transport was inhibited by D-xylose and D-ribose, although not as effective as hexoses. However, L-xylose had a little effect on 2dGlc transport in the Sf9 cells, indicating that the transport is stereoselective. Unlike the human erythrocyte-type glucose transport system, D-ribose had a somewhat greater apparent affinity for the Sf9 cell transporter than D-xylose. It is therefore concluded that Sf9 cells contain an endogenous sugar transport activity that in some aspects resembled the human erythrocyte-type counterpart, although the Sf9 and human transport systems do differ in their affinity for cytochalasin B.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.11b
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• pp.147-147
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• 2002

In this study, a mechanism by which glucose level modulates glucose transport in Jurkat cells was investigated. Glucose uptake was more efficient in the cells cultivated in low glucose (2.5 mM) medium than that grown in high glucose (20 $\mu$M) medium. Vmax (0.74 n㏖/10$^6$ cells$\cdot$min) of glucose uptake measured with the cells grown in the low glucose medium was higher than the one (1.06 n㏖/10$^6$ cells$\cdot$min) in the high glucose medium while Km was almost consistent through the change of glucose levels, indicating the increase of glucose transporter number.