• 한국어류학회지
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• 제20권2호
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• pp.81-89
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• 2008

메기, Silurus asotus에서 포식과 기아가 생존과 성장, 내장괴지수, 간중량지수, 생식소중량지수, 외형계측형질 변화 및 혈액 parameter들에 미치는 영향을 파악하기 위해 80일동안 기아 실험을 수행하였다. 기아군의 생존과 성장은 포식군의 생존과 성장에 비해 낮았다(P<0.05). 기아는 체중성장률, 일간성장률과 비만도에서 성장 정지 효과를 나타낸 반면, 포식군은 지속적인 성장을 보였다. 외형 계측형질 중복강 부위 횡단길이 관련에서 포식군은 실험 시작시와 차이를 보이지 않았으나, 기아군은 차이를 나타냈다 (P<0.05). 실험 기간 중 혈액성상(cortisol, glucose, AST, ALT, RBC 및 hemoglobin)에서 포식군과 기아군 사이에 유의한 차이를 보였다. 본 연구 결과, 포식과 기아 파악에 사용된 모든 parameter들은 본 종의 영양 상태 확인의 지표로 유용하며, 양식 메기의 성장 시기 평가 결정에 유용하리라 사료된다.

• 한국수산과학회지
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• 제49권5호
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• pp.620-627
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• 2016

A 60-day experiment was conducted to determine the effect of feeding and starvation on the survival, growth, and blood parameters of red sea bream Pagrus major. The starved group was not fed during the first 32 days of the experiment and was then fed for 28 days. The growth rate of the starved fish group was significantly lower than that of the fed fish group. Starvation resulted in growth retardation and reductions in final body weight, growth rate, specific growth rate, and condition factor, whereas the fed fish group grew well and maintained a good condition. The growth rate of the starved group that was refed was higher than that of the fed group. Red blood cells, hematocrit, and hemoglobin showed no significant differences between the fed and starved groups. The cortisol and glucose levels of the fed group of juveniles were higher than those of the starved group. The cortisol levels of the starved group of adults were higher than those of the fed group, whereas the glucose levels of the starved group were lower than those of the fed group. These results suggest that the survival, growth, and hematological parameters used to reflect starvation and feeding in this study provide a useful index of physiological response and survival rate in red sea bream.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1276-1284
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• 2012

A 12-wk experiment was conducted to examine the hematological changes, survival, and respiratory exchange in the olive flounder, Paralichthys olivaceus, during starvation. The growth, survival and respiratory exchange rates of the starved group were lower than those of the fed group during the experiment. Blood analysis, including hematocrit, hemoglobin, red blood cells, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and mean corpuscular volume, did not differ significantly (p>0.05) between the fed and starved groups at the end of the experiment. There were no significant differences in plasma cortisol, glucose, $Na^+$, $Cl^-$, $K^+$, or aspartate aminotransferase between the fed and starved groups (p>0.05). Alanine aminotransferase levels were higher in the starved group than in the fed group, whereas plasma osmolality was lower in the starved group than in the fed group. It was shown that starved fish had various problems after four weeks, which did not occur in the fed group. Long-term starvation is infrequent in aquaculture farms. However, starvation studies of this kind are very useful for a basic understanding of how physiological changes affect fish health, life expectancy, and growth.

• Asian-Australasian Journal of Animal Sciences
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• 제29권11호
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• pp.1593-1600
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• 2016

This research was conducted to investigate the physiological consequences of undernourished yak. Twelve Maiwa yak ($110.3{\pm}5.85kg$) were randomly divided into two groups (baseline and starvation group). The yak of baseline group were slaughtered at day 0, while the other group of yak were kept in shed without feed but allowed free access to water, salt and free movement for 9 days. Blood samples of the starvation group were collected on day 0, 1, 2, 3, 5, 7, 9 and the starved yak were slaughtered after the final blood sample collection. The liver and muscle glycogen of the starvation group decreased (p<0.01), and the lipid content also decreased while the content of moisture and ash increased (p<0.05) both in Longissimus dorsi and liver compared with the baseline group. The plasma insulin and glucose of the starved yak decreased at first and then kept stable but at a relatively lower level during the following days (p<0.01). On the contrary, the non-esterified fatty acids was increased (p<0.01). Beyond our expectation, the ketone bodies of ${\beta}$-hydroxybutyric acid and acetoacetic acid decreased with prolonged starvation (p<0.01). Furthermore, the mRNA expression of lipogenetic enzyme fatty acid synthase and lipoprotein lipase in subcutaneous adipose tissue of starved yak were down-regulated (p<0.01), whereas the mRNA expression of lipolytic enzyme carnitine palmitoyltransferase-1 and hormone sensitive lipase were up-regulated (p<0.01) after 9 days of starvation. The phosphoenolpyruvate carboxykinase and pyruvate carboxylase, responsible for hepatic gluconeogenesis were up-regulated (p<0.01). It was concluded that yak derive energy by gluconeogenesis promotion and fat storage mobilization during starvation but without ketone body accumulation in the plasma.

• 한국어류학회지
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• 제29권3호
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• pp.165-175
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• 2017

본 연구는 하절기에 지속적으로 발생하는 고수온, 적조와 같은 피해를 최소화하기 위한 방안으로 해상가두리 ($6m{\times}6m{\times}6m$) 절식 사육을 통해 참돔의 생존율, 성장, 성장 회복 그리고 생리반응을 조사하였다. 6주간의 절식 후 4주간의 먹이 공급을 통한 연구결과 생존율은 섭식구 94.4%, 절식구 98.1%로 나타났다. 체중 성장률은 절식구에서 $-12.4{\pm}3.2%$ 성장을 보여 체중의 유의한 감소를 보였지만, 먹이 공급 후 $36.7{\pm}6.8%$의 빠른 성장 회복을 나타냈다. 혈액분석을 통한 절식구의 영양상태 또한 섭식구에 비해 유의하게 감소하였지만 먹이 공급 후 개시 시 수준으로 빠르게 회복하였다. 또한 스트레스 지표를 분석한 결과 절식기간 섭식구의 Hb, Ht의 수치가 증가하였으며, 섭식구의 급격한 혈액성상의 변화는 하절기 해상가두리 환경에서 절식구보다 섭식구가 스트레스에 더 민감한 것으로 나타났다. 따라서 본 연구의 생존율, 성장 회복, 혈액성상을 통한 결과를 토대로, 고수온과 적조에 노출되는 하절기에 피해를 최소화하기 위한 가두리 양식어류 관리 방안으로 일정기간 절식이 활용 가능할 것으로 여겨진다.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.678-682
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• 2005

The promoter region of a carbon starvation gene isolated from Pseudomonas putida was cloned and analyzed for its potential use for in situ bioremediation and bioprocessing. We constructed a recombinant plasmid pMKD101 by cloning the 0.65 kb promoter region of the gene into the promoter proving vector, pMK301, which contains the lacZ for ${\beta}$-galactosidase activity as a reporter gene. pMKD101 was transformed into the wild-type P. putida MK1, resulting in P. putida RPD101, and analyzed for ${\beta}$-galactosidase activity under different culture conditions. When RPD101 was grown on the minimal medium plus $0.1\%$ glucose as a sole carbon source in batch cultures, ${\beta}$-galactosidase activity was found to be 3.2-fold higher during the stationary phase than during the exponential phase. In chemostat cultures, ${\beta}$-galactosidase activity was found to be 3.1-fold higher at the minimal growth rate (dilution rate=$0.05\;h^{-1}$) than at the maximal growth rate (dilution rate=$0.173;h^{-1}$). The results suggest that a carbon starvation promoter can be utilized to maximize the expression of a desired gene under nutrient limitation.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2007년도 춘계학술발표회
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• pp.65-73
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• 2007

The objective of this study is to understand how regulatory mechanisms respond to sugar status for more efficient carbon utilization and source-sink regulation in plants. So, we need to identify and characterize many components of sugar-response pathways for a better understanding of sugar responses. For this end, genes responding change of sugar status were screened using Arabidpsis cDNA arrays, and confirmed thirty-six genes to be regulated by sucrose supply in detached leaves by RNA blot analysis. Eleven of them encoding proteins for amino acid metabolism and carbohydrate metabolism were repressed by sugars. The remaining genes induced by sugar supply were for protein synthesis including ribosomal proteins and elongation factors. Among them, I focused on three hydrolase genes encoding putative $\beta$-galactosidase, $\beta$-xylosidase, and $\beta$-glucosidase that were transcriptionally induced in sugar starvation. Homology search indicated that these enzymes were involved in hydrolysis of cell wall polysaccharides. In addition to my results, recent transcriptome analysis suggested multiple genes for cell wall degradation were induced by sugar starvation. Thus, I hypothesized that enzyme for cell wall degradation were synthesized and secreted to hydrolyze cell wall polysaccharides producing carbon source under sugar-starved conditions. In fact, the enzymatic activities of these three enzymes increased in culture medium of Arabidopsis suspension cells under sugar starvation. The $\beta$-galactosidase encoded by At5g56870 was identified as a secretory protein in culture medium of suspension cells by mass spectrometry analysis. This protein was specifically detected under sugar-starved condition with a specific antibody. Induction of these genes was repressed in suspension cells grown with galactose, xylose and glucose as well as with sucrose. In planta, expression of the genes and protein accumulation were detected when photosynthesis was inhibited. Glycosyl hydrolase activity against galactan also increased during sugar starvation. Further, contents of cell wall polysaccharides especially pectin and hemicellulose were markedly decreased associating with sugar starvation in detached leaves. The amount of monosaccharide in pectin and hemicellulose in detached leaves decreased in response to sugar starvation. These results supported my idea that cell wall has one of function to supply carbon source in addition to determination of cell shape and physical support of plant bodies.

• Journal of Yeungnam Medical Science
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• 제1권1호
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• pp.89-93
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• 1984

선택수술이 계획된 4세이하, 체중 18kg이하 소아 환자 38명을 대상으로 하여 수술전 병실에서 측정된 혈당치를 대조치로하고, 8시간 정도의 금식후 수술실로 오기 1시간 전부터 비포도당액인 Hartmonn's solution을 공급한 I군과 포도당이 함유된 Hartmann's dextrose solution을 공급한 II군으로 나누어 각각 혈당치를 측정하고 또 수술 1시간후의 혈당치를 측정하여 혈당치변화를 관찰하였던 바 다음과 같은 결론을 얻었다. 1) I군에서는 대조치 $95.4{\pm}13.3mg%$에 비해 8시간 금식후의 혈당치는 $85.6{\pm}12.2mg%$로 감소했으며, 수술후 1시간에 있어서의 혈당치는 $119.2{\pm}33.0mg%$로 대조치에 비해 유의한 증가를 보였다. 2) II군의 경우에는 대조치 $94.6{\pm}28.5mg%$에 비해 8시간 금식후의 혈당치는 $99.8{\pm}21.8mg%$)로 약간 증가되었고, 수술후 1시간의 혈당치는 $122.3{\pm}27.3mg%$로 유의한 증가를 보였다. 이상의 결과로 보아 신아에서 약 8시간정도의 금식으로는 저혈당이 초래되지 않으며, 또한 수술중에 혈당이 증가되므로 소아수술시 수술중 혈당유지를 위한 포도당액의 투에는 필요치 않은 것으로 사료된다.

• KSBB Journal
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• 제13권6호
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• pp.681-686
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• 1998

5L 발효기에서 글루코스의 투입방법과 배지의 초기 글루코스 농도에 따른 유가식 배양에 의한 S. marcescens의 증식에 미치는 영향에 대해서 연구하였다. 발효기 조건을 일정하게 유지시켜준 각 유가식 배양에서의 최종란체량이 회분식배양에 비해서 훨씬 높았으며, 유기식 배양종에서도 기질인 글루코스의 공급을 달리하였을 때, 최종균체량에서 차이를 나타내었다. 회분식 배양과 유가식 배양중에서 글루코스의 일정비율공급밤법, 글루코스 결핍상태에서의 일정비율공급방법 그리고 지수적 공급방법의 최종균체량은 1.40, 5.07, 6.93 및 7.60g/L 이고, 그 때의 배양시간은 40, 41. 24 및 40hr이었다. 그리고 균체 생산성은 0.035, 0.124, 0.239 및 0.1909/L.h 이었다. 최종 균체량만을 가지고 비교하였올 경우에는 유가식 배양줌에서 지수적 공급방법이 가장 효율적이지만 당위시간당 균체 생산성에서는 글루코스 결핍 상태에서의 일정비율공급방법이 1.52배 정도 높게 나타냈으므로 가장 효과적인 배양 방법임을 알 수 있다. 용존산소와 글루코스 루입시가의 상관관계에서 나타난 바와 같이 글루코스 결핍상태에서 일정비율로 공급한 방법과 지수적으로 공급한 방법에서 좋은 일치를 보여주었다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권9호
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• pp.1360-1367
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• 2014

Triplicate groups of fed and starved olive flounder, Paralichthys olivaceus (body weight: $119.8{\pm}17.46$ g), were examined over 42 days for physiological changes using hematological, biochemical, and non-specific immune parameters. No significant differences in concentrations of blood hemoglobin and hematocrit and plasma levels of total cholesterol, aspartate aminotransferase, alanine aminotransferase, glucose, and cortisol were detected between fed and starved groups at any sampling time throughout the experiment. In contrast, plasma total protein concentrations were significantly lower in starved fish than in fed fish from day 7 onwards. Moreover, plasma lysozyme concentrations were significantly higher in starved flounder from day 21 onwards. This result confirms that the response of olive flounder to short-term (less than about 1.5 months) starvation consists of a readjustment of metabolism rather than the activation of an alarm-stress response. The present results indicate that starvation does not significantly compromise the health status of fish despite food limitation.