• KSBB Journal
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• 제6권1호
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• pp.15-25
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• 1991

The optimal glucose concentration for the high-density culture of recombinant yeasts was obtained using dynamic simulation. An adaptive and predictive algoritilm complimented by the rule base was proposed for the control of the fed-batch fermentation process. The measurement of process variables has relatively long sampling period and relatively long time delay characteristics. As one of the solution on these problems, prediction techniques and rule bases were added to a classical recursive identification and control algorithm. Rule bases were used in the determination of control input considering the difference between the predicted value and the measured value. A mathelnatical model was used in the estimation and interpretation of the changes of state variables and parameters. Better performances were obtained by employing the control algorithm proposed in the present study compared to the conventional adaptive control method.

• Fibers and Polymers
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• 제7권3호
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• pp.241-244
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• 2006

In this paper, the effect of cellulase treatment was evaluated by means of phenol-sulphuric acid method. This method was performed by determining sugar liberation in the treatment bath with the amount expressed in glucose equivalent. As compared with conventional method, the measurement of amount of sugar liberated gave a more reliable and accurate result than the weight loss method. It was found that although the weight loss of cellulose became negligible when the treatment was done under agitation-free condition, the amount of sugar liberated was still readily measurable.

• The Korean Journal of Ecology
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• 제15권3호
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• pp.287-296
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• 1992

To determinate self-purification constants of pollutants loaded in the ecosystems, the self- purification process was formulated, and a measurement method of the self-purification constants was derived. $C=C_0e^{-st}$ When $C_0$ is the initial pollutant amounts loaded in a ecosystem, and C is the rest pollutant amounts after the time, t, the equation of the self-purification, s, is $s=\frac{P}{C}$ When in aquatic ecosystem, $C_0$ is the initial polluant amounts loaded in water body, and Cis the rest pollutation amounts after the time, t, the self-purification constant, s, is $s=(\frac{\ln C_0-\ln C}{t}$ Self-purification constants of pine and oak forests at kwangneung in kyonggido were 0.07 and 10 respectively, of BOD in gokneung stream in kyonggido was 0.51, and of glucose and phosphate in pools on the stone in mt.jiri were 0.49 and 15.19 respectively.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 춘계학술대회 논문집 센서 박막재료 반도체 세라믹
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• pp.28-32
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• 2003

본 논문은 시료의 양을 최소화하고 혈중 요소 및 혈당 농도에 대한 정확한 측정을 위하여 최근에 개발된 전기화학형 마이크로 전극형 센서의 간략한 소개 및 전기적인 특성에 관하여 요약하는 것에서 출발하여 센서로부터 전기신호를 발생시키는 signal conditioning 회로의 설계, microprocessor를 이용한 digital 값으로의 변환 및 data 취득, 취득한 data를 측정된 혈당량 및 요소량으로의 변환 저장 display까지의 휴대용 계측기의 prototype 개발에 관하여 기술한다. 이 계측기는 미량의 혈액채취 한번으로 농도를 측정하는 측정모드와, 측정된 data를 찾아보기 위한 검색모드로 작동시킬 수 있다. Microprocessor의 기능을 최대한 사용하여 측정기의 크기를 소형화하였으며 LCD(Liquid crystal display)틀 채택하는 등 저전력 회로로 구현하였다.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1993년도 학술대회지
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• pp.164-170
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• 1993

Panax ginseng has been reported to protect animals or to help them recover from physical, chemical, or biological stress. The antistress effects of ginseng were evaluated through the measurement of adrenal ascorbic acid, rectal temperature, and plasma level of glucose. lipids and corticosterone. During the treadmill experiments of the antifatigue study, the groups of rats receiving P. ginseng or P. quinquefolius extracts were consistently found to leave fewer fecal boli on the wheel compared with controls. This phenomenon may be due to the reported antistress effects of ginseng. Another possibility could be that the Panax species examined produced anticholinergic effects which in turn inhibited the production of fecal boli. After an anticholinergic study. employing physostigmine and atropine as controls, anticholinergic effect was found not essential for the decrease of fecal boli number left on the wheels during antifatigue studies. The results were consistent with the antistress activity reported previously. Even though the active constituents responsible for the antistress effects of ginseng remained to be determined, the fecal boli counts for stressed rats can be employed as a new protocol for evaluating the antistress effects of ginseng.

• 대한영양사협회학술지
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• 제8권2호
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• pp.185-198
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• 2002

The purpose of this study was to compare food habits and nutrient intakes with body mass index of hypertensive patients commuting to a Local Health Center. A total of 85 patients were divided into two groups according to BMI. The non-obese group comprised 43 subjects with BMI below 25㎏/$m^2$ , while the obese group comprised 42 subjects with BMI above 25㎏/$m^2$ . All Subjects were interviewed for general characteristics, food habits, clinical characteristics, effort for health maintenance and the knowledge of hypertension and nutrition. Anthropometric assessments such as weight, height, waist-hip ratio and biochemical measurement of blood urea nitrogen, creatinine, triglyceride, total cholesterol, HDL-cholesterol and fasting blood glucose(FBG) were obtained from subjects. In general characteristics, smoking, drinking, exercise, and hypertension status were not significantly different between the two groups. Food habits and the means of daily energy and nutrients were not significantly different between the two groups. An analysis of the percentage of RDA(Recommended Dietary Allowances of Korea, 2000) consumed by patients showed that but for ascorbic acid and phosphorus, all nutrients were below the RDA. And intakes of vitamin $B_1$(P<0.001), vitamin $B_2$(P<0.01), niacin(P<0.001) of the obese group were significantly lower than that of the non-obese group. Blood urea nitrogen, creatinine, triglyceride, total cholesterol, HDL-cholesterol and fasting blood glucose were in the normal range and there was not a significant difference in the two groups. Therefore, more effective nutrition education programs about exercise, smoking, caloric intake, vitamins and minerals are required for hypertensive patients commuting to Local Health Center.

• 센서학회지
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• 제9권3호
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• pp.203-207
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• 2000

ISFET 포도당센서는 전류법적인 엑츄에이션(amperometric actuation)기법을 도입하여 감도 향상에 큰 이점을 얻을 수 있었다. 그러나 이러한 측정법은 측정 후, 감지막 내부와 외부사이에 화학적 평형상태를 유지하려는 수소이온의 이동 때문에 초기 출력 값을 회복하는 데 많은 시간을 필요로 한다. 이러한 회복시간 지연문제는 센서의 실용화 장애 요인 중 하나이다. 본 논문에서는 백금작업전극에 환원전위를 인가하여 수산화($OH^-$)이온을 인위적으로 발생시킴으로써 감지막 내의 수소이온 농도를 조절하는 새로운 방법을 제안하였다. 제안된 방법으로 실험한 결과, 기존에 수십 분 소요되었던 회복시간을 2분 이내로 단축하였다.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.131-137
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• 2001

Previous work has identified that only the catabolite responsive element A (creA; previously called cre-2) out of two potential cre sequences (cre-1: nucleotide +160 to +173 and cre-2: +173 to +186), recognized within the coding region of the xylanaseA gene (xynA) of Bacillus stearothermophilus No.236, was actually, was actually involved in the carbon catabolite repression(CCR) of xynA expression in B. subtilis. However, the level of CCR of xynA expression in the original B.stearothermophilus No.236 strain (70-fold repression). Therefore, to search for an additional cre element in the promoter region, the upstream region of the xynA gene was subcloned by chromosome walking, and as a result, another potential cre element (nucleotide -124∼-137; designated creB) was recognized in this region. The cre-like sequence revealed a high homology to the cre consensus sequence. The xylanase activity of B. subtilis MW15 bearing pWPBR14 (containing creA and creB) cultured in a medium containing xylose as the sole carbon source was about 7.7 times higher than that observed for the same culture containing glucose. B. subtilis MW15 bearing pWPBR23 (containing only creA) produced an activity about 2.4 times higher. This pattern of CCR was confirmed using derivatives of xynA::aprA fusion plasmids. Furthermore, a measurement of the amounts of the xynA transcript showed a similar pattern as that for the production of xylanase. In addition, the synthesis of xylanase in B. subtilis QB7115 [a catabolite control protein A (ccpA) mutant strain] carrying pWPBR14 was almost completely relieved from glucose repression. Together, these results lead to a conclusion that the CCR of the expression of the xynA gene is mediated by CcpA binding at creA and creB sites in B. subtilis.

• 환경생물
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• 제17권4호
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• pp.415-420
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• 1999

용존 유기물의 농도를 BOD로 신속히 측정하여 폐수 처리 공정에 feed back할 수 있는 Candida BOD sensor를 이용하여 BOD를 측정하였다. Glucose, acetic acid, aspartic acid 및 glutamic acid에 대한 sensitivity는 lactose, sucrose및 lactic acid의 sensitivity보다 더 높게 나타나서 오염물질의 종류에 따라 BOD sensor의 출력 신호가 다르게 제시되었다.$Zn^{2+}$내성주가 충전된 BOD sensor는 중금속 비내성주의 경우와 유사한 정도의 감응도를 나타내었으나 $Cd^{2+}$ 내성주를 사용하였을 때는 매우 낮은 sensitivity를 보였다. 수용액의 $Cd^{2+}$의 농도가 증가함에 따라 중금속 비내성주와 $Zn^{2+}$ 내성주의 sensor 의 sensitivity는 거의 일정하였으나 $Cd^{2+}$ 내성주의 BOD sensor의 경우에는 sensitivity가 오히려 감소하였다. 반면 수용액 중의 $Zn^{2+}$ 의 농도가 증가함에 따라서는 중금속 비내성주, $Zn^{2+}$ 내성주 및 $Cd^{2+}$ 내 성주 모두 거의 동일한 감응도를 나타내었다.

• Nutrition Research and Practice
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• 제10권2호
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• pp.175-181
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• 2016

BACKGROUND/OBJECTIVES: The anti-obesity effect of quercetin-rich onion peel extract (OPE) was suggested in rats, but information from human studies is limited. This study aimed to investigate the effects of OPE on the body composition of overweight and obese subjects. MATERIALS/METHODS: In this 12-week, randomized, double-blind, placebo-controlled study, parallel clinical trials were performed in overweight and obese Korean subjects. Randomly assigned subjects were instructed to take daily either the placebo (male, 6 and female, 30) or OPE capsules containing 100 mg of quercetin (male, 5 and female, 31). Body composition was measured by using bioimpedance and dual-energy X-ray absorptiometry (DXA). Resting energy expenditure (REE) and respiratory quotient (RQ) were evaluated by using indirect calorie measurement methods. Fasting blood levels of glucose, insulin, lipids, and leptin were determined. RESULTS: Quercetin-rich OPE supplementation significantly reduced the weight and percentage of body fat as measured by DXA (P = 0.02). These effects were not shown in the control group. Levels of blood glucose (P = 0.04) and leptin (P = 0.001 for placebo, P = 0.002 for OPE) decreased in both groups. Significant increases in REE and RQ were observed in both groups (P = 0.003 for placebo, P = 0.006 for OPE) and in the OPE group alone (P = 0.02), respectively. CONCLUSIONS: Quercetin-rich OPE supplementation changed the body composition of the overweight and obese subjects. This result suggests a beneficial role of the anti-obesity effect of OPE human subjects.