• The Korean Journal of Mycology
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• v.47 no.3
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• pp.275-280
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• 2019

We isolated fungal spores belonging to the phylum Glomeromycota from the rhizospheres of Smilax china, cultured in a greenhouse. We identified the isolated spores using sequence analysis of 18S partial rDNA region, internal transcribed spacer and 28S rDNA regions. We confirmed 2 unreported spores of Glomeromycota fungal species, Diversispora eburnea and Paraglomus laccatum. Here, we described the morphological characteristics and results of phylogenetic analysis of the confirmed species.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.425-431
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• 2021

New species of arbuscular mycorrhizal fungi (Glomeromycota), Acaulospora jejuensis, was isolated from rhizosphere soils of Miscanthus sinensis in the grassland in Jeju Island of Korea. The species was identified using the morphological characteristics of the spores and the molecular phylogenetic analysis using partial DNA sequences from small subunit rDNA (SSU), internal transcribed spacer (ITS), and large subunit rDNA (LSU). Phylogenetic analysis placed the fungal species in a distinct clade within genus Acaulospora. Also, the species exhibited the morphological characteristics distinct from the other members of the genus. Therefore, Acaulospora jejuensis was described as a novel species from Korea.

• Mycobiology
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• v.46 no.4
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• pp.341-348
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• 2018

A new species of arbuscular mycorrhizal fungi (Glomeromycota), Acaulospora koreana, was isolated from forest soils in South Korea. This novel fungus was collected from the rhizosphere of Lindera obtusiloba and Styrax obassia in forest and propagated with Sorghum bicolor in pot. Morphological characteristics of spores of A. koreana are rarely distinguished from Acaulospora mellea, which is reported as one of the most abundant mycorrhizal species in Korea. However, molecular evidence of rDNA sequence using improved primers for glomeromycotan fungal identification strongly supported that A. koreana is different from A. mellea but also any other species belonging to the genus Acaulospora. This is the first novel glomeromycatan fungus introduced in South Korea, but it suggests that there is a high possibility for discovering new arbuscular mycorrhizal fungi considering the abundance of plant species and advanced phylogenetic analysis technique.

• Mycobiology
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• v.51 no.3
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• pp.164-168
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• 2023

This study examined the effects of myristate on an asymbiotic culture of Rhizoglomus intraradices, a species of arbuscular mycorrhizal fungi (AMF; Glomeromycota). Mycelial growth and sporulation in a modified medium containing myristate were observed. The findings demonstrated that myristate induced R. intraradices spore formation, with daughter spores having a smaller diameter than the parent spores. This observation is consistent with previous studies on other Rhizoglomus species. Further studies are needed to investigate the potential for continuous culture, mass production using daughter spores, and the application of AMF colonization techniques in plants.

• The Korean Journal of Mycology
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• v.48 no.3
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• pp.209-215
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• 2020

Spores of arbuscular mycorrhizal fungi (phylum Glomeromycota) were isolated from rhizosphere soil collected in Korea. We identified the morphological characteristics of the spores and performed a phylogenetic analysis using the rDNA 18S, 5.8S, and 28S regions. To the best of our knowledge, we confirm the presence of three species of glomeromycotan fungi previously not reported in Korea, namely Acaulospora delicata, Dentiscutata colliculosa, and Racocetra alborosea. We described the morphological characteristics and results of phylogenetic analysis of these species.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.978-987
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• 2015

Aluminum (Al) toxicity is one of the greatest limitations to agriculture in acid soils, particularly in tropical regions. Arbuscular mycorrhizal fungi (AMF) can supply plants with nutrients and give protection against Al toxicity. The aim of this work was to evaluate the effects of soil liming (i.e., reducing Al saturation) on the AMF community composition and structure in the roots of maize lines contrasting for Al tolerance. To this end, we constructed four 18S rDNA cloning libraries from L3 (Al tolerant) and L22 (Al sensitive) maize lines grown in limed and non-limed soils. A total of 790 clones were sequenced, 69% belonging to the Glomeromycota phylum. The remaining sequences were from Ascomycota, which were more prominent in the limed soil, mainly in the L3 line. The most abundant AM fungal clones were related to the family Glomeraceae represented by the genera uncultured Glomus followed by Rhizophagus and Funneliformis. However, the most abundant operational taxonomic units with 27% of the Glomeromycota clones was affiliated to genus Racocetra. This genus was present in all the four libraries, but it was predominant in the non-limed soils, suggesting that Racocetra is tolerant to Al toxicity. Similarly, Acaulospora and Rhizophagus were also present mostly in both lines in non-limed soils. The community richness of AMF in the non-limed soils was higher than the limed soil for both lines. The results suggest that the soil Al saturation was the parameter that mostly influences the AMF species composition in the soils in this study.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.255-261
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• 2014

In this study, we summarized previous studies on diversity and distribution of arbuscular mycorrhizal fungi (AMF) for last 30 years in Korea. According to a review of the literature concerning AMF in Korea, 14 genera and 89 species have been recorded. Host plants for AMF are very diverse and include crop species and woody plants in natural forests. Based on the achievements of the last 30 years of study on AMF, we anticipate that relatively more intensive studies of the functional and genetic diversity of AMF will be conducted.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.517-524
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• 2021

Red pepper seedlings were inoculated either alone or with a mixture of all five species of arbuscular mycorrhizal fungi (AMF). After 10 weeks of growth in the greenhouse, the seedlings were transplanted into fields and cultivated without chemical fertilizers and pesticides for 10 weeks. The results showed that plant growth was significantly increased under both greenhouse and field conditions, suggesting that AMF inoculation has a positive effect on the growth of Capsicum annuum and improves the physical properties of the soil by increasing the concentration of glomalin. The application of AMF can positively contribute to sustainable agriculture by reducing the use of chemical fertilizers while increasing crop growth.

• The Plant Pathology Journal
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• v.23 no.4
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• pp.287-294
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• 2007

Endophytic fungi were isolated from surface sterilized root tissues of Aralia elata and Aralia continentalis, collected from farmer's field in Chungnam province, Republic of Korea, in 2005. Based on ITS sequence analysis, 24 fungal genera were characterized from 359 isolates, belonging to 22 Ascomycota, 1 Glomeromycota and 1 Oomycota. Strumella, Rhizopycnis and Entrophospora in A. elata and Rhizopycnis and Leptosphaeria in A. continentalis were the most abundant taxa. Out of 24 genera, Entrophospora, Leptodontidium, Neoplaconema, Paraconiothyrium, Rhizopycnis, Strumella and Tumularia were new to Korea. A total of 110 isolates were tested for antifungal activities against six plant pathogenic fungi. Out of these, 39 isolates showed antifungal activity against at least one plant pathogenic fungi. Four isolates of Pyrenochaeta, 1 isolate of Entrophospora and 1 unidentified fungus strongly inhibited the growth of six plant pathogenic fungi.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.306-311
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• 2014

In this study, arbuscular mycorrhizal fungi (AMF) were isolated and cultivated from rhizospheres of various plants in Korea. Five species belonging to four genus were identified based on spore morphology and 18S rDNA sequence analysis: Acaulospora cavernata Claroideoglomus luteum, Diversispora aurantium, D. trimurales, Rhizophagus irregularis. These fungal species have not been cultivated or reported to date in Korea, Morphological description and phylogenetic analysis for each species were presented. This result could be useful for research of AMF diversity in Korea.