• BMB Reports
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• v.41 no.1
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• pp.35-40
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• 2008

The molten globular conformation of V26A ubiquitin (valine to alanine mutation at residue 26) was studied by nuclear magnetic resonance spectroscopy in conjunction with amide hydrogen/deuterium exchange. Most of the amide protons that are involved in the native secondary structures were observed to be protected in the molten globule state with the protection factors from 1.2 to 6.7. These protection factors are about 2 to 6 orders of magnitude smaller than those of the native state. These observations indicate that V26A molten globule has native-like backbone structure with marginal stability. The comparison of amide protection factors of V26A ubiquitin molten globule state with those of initial collapsed state of the wild type ubiquitin suggests that V26A ubiquitin molten globule state is located close to unfolded state in the folding reaction coordinate. It is considered that V26A ubiquitin molten globule is useful model to study early events in protein folding reaction.

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.831-841
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• 1997

The study was aimed at identifying the globule leukocytes (GL) of tracheal mucosa layer of Korean native cattle showing symptom of pneumonia which have died as enterotoxemia and normal Korean native cattle in Kyungpook local area. In another set of experiment, Isolated Klebsiella pneumoniae from suddenly died cattle specimens was subjected to rat for a determining globule leukocyte appearance by using histochemical and immunohistochemical method. In histochemical study, globule leukocytes generally was existed in all the case of postmortem of Korean native cattle and 3 heads of slaughtered cattle which showing symptoms of pneumonia and it showed significant increase in tracheal mucosa of rats experimentally infected with Klebsiella pneumoniae. These increased number of globule leukocytes was moderately remained on early infection stage and gradually decreased in timedependent manner after infection. The granule patterns were also determined as an acidmucopolysaccharide. In immunohistochemical study, serotonin intensity in the treacheal mucosaepithelial cells of rat experimentally infected observed a strong immunoreactivity during early infection and gradually decreased in dependent of infection stage while no IgE immunoreactivity observed. These data show that globule leukocytes were increased in a pneumonia, therefore it was considered as a valuable cell that was associated with early stage of inflammatory response.

• BMB Reports
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• v.37 no.6
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• pp.676-683
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• 2004

The conformational properties of hydrophobic core variant ubiquitin (Val26 to Ala mutation) in an acidic solution were studied. The intrinsic tryptophan fluorescence emission spectrum, far-UV and near-UV circular dichroic spectra, the fluorescence emission spectrum of 8-anilinonaphthalene-1-sulfonic acid in the presence of V26A ubiquitin, and urea-induced unfolding measurements indicate this variant ubiquitin to be in the partially folded molten globule conformation in solution at pH 2. The folding kinetics from molten globule to the native state was nearly identical to those from the unfolded state to the native state. This observation suggests that the equilibrium molten globule state of hydrophobic core variant ubiquitin is an on-pathway folding intermediate.

• Journal of The Korean Astronomical Society
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• v.22 no.1
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• pp.63-79
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• 1989

Current estimates, based on the same star-count analysis, of the distance to the globule Bamard 361 range from 300 pc to 650 pc. All the problems associated with the estimates have been fully rectified in this study, and a modification has been made to the classical Wolf diagram to improve the accuracy in the distance determination. A reference field was carefully selected close to the globule but well outside the globule boundary, and star counts for this field were performed on the blue POSS plate in order to set up the reference magnitude sequence appropriate to the general area of B 361. From the reference sequence, the stellar density function has been derived specifically for the direction toward the globule. Correction was made for the general interstellar extinction, and the luminosity function with the Wielen's dip was adopted. The resulting density function clearly reveals the existence of the local Cygnus-Orion arm in the direction of B 361 at about 700 pc away from the Sun. Analysis of the star-count data for the program field locates the globule at distance $600{\pm}50$ pc ; thus, the globule is an object located in the Cygnus-Orion arm, residing somewhat toward its leading edge.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2001.10a
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• pp.236-239
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• 2001

In thixoforming, the globule size is determined by the hoding time of last reheating stage. In this study, some experiments to investigate the relationship between the mechanical properties and the holding time were performed A357, A390 and A3l9 alloys are used in this study. This paper shows the evolution of the microstructure according to the holding time of last reheating stage. Tensile test was performed for each reheating condition to examine the effect of globule size.

• Transactions of Materials Processing
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• v.11 no.2
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• pp.155-164
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• 2002

One of the important steps on semi-solid forming Is the reheating process of raw materials to the semi-solid state. This Process is not only necessary to achieve the required SSM billet state, but also to contro1 the microstructure of the billet. In reheating process, the globule size is determined by the holding time of last heating stage. Therefore, some experiments to investigate the relationship between the mechanical properties and the holding time in the last heating stage was performed. The alloys used in this experiment were 357, 319 and A390 alloys. The experiments of reheating were performed by using an Induction heating system with the capacity of 50kw. This paper shows the evolution of the microstructure according to the holding time of last reheating stage. Furthermore, to evaluate the effect of globule size controlled by holding time in last heating stage uniaxial tension test was performed. The strain-stress curves were plotted according to the holding time.

• Journal of Dairy Science and Biotechnology
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• v.14 no.2
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• pp.175-183
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• 1996

• Korean Journal of Food Science and Technology
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• v.22 no.4
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• pp.369-372
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• 1990

Although the measurement of oil globule size distribution is necessary to detect the process of demulsification, the reasonable methodology for its measurement has not been suggested because the solution of soymilk contains insoluble soybean particle and the protein to interfere with the detection of oil globule or oil content. The oil globule size distribution was measured by the homogeneous suspension and cumulative method under gravitational force or centrifugal force, which were modified with Stokes' low. The geometric mean diameter of oil globules in this soymilk was $033{\mu}m\;and\;031{\mu}m$ under gravitational method and centrifugal method, respectively. The differences of oil globule size distribution in the solutions emulsified by different pressures were detected by this method. The mean diameter of the solutions treated at higher pressure was shifted to smaller size and the distribution pattern of the solutions at higher pressure became more compact around the mean diameter.

• Journal of the Korean Applied Science and Technology
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• v.32 no.3
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• pp.536-545
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• 2015

This study aimed to investigate the effect of various emulsifiers on the formation of nanoemulsions and their stability properties. MCT (medium chain triglyceride) nanoemulsions were prepared (10 wt% oil, 10 wt% emulsifiers, 20 mM bis-tris, pH 7) with emulsifier such as Tween 20 (Polyoxyethylene(20) sorbitan monolaurate), Almax 3800 (Sorbitan monooleate), soy lecithin, and SSL (sodium stearoyl lactylate) and changes in fat globule size with respect to storage period and stability properties by Turbiscan were investigated. In case of control nanoemulsion with 10 wt% Tween 20, the initial fat globule size was 89.0 nm and 113.4 nm after 28 day of storage and this large increase (ca. 24 nm) was thought to be caused by Ostwald ripening. When Tween 20 was partially replaced with Almax 3800, lecithin and SSL in nanoemulsions, their physicochemical properties (i.e., fat globule size and stability) were changed accordingly. In general, the intial fat globule size was decreased with increasing the concentration of the emulsifiers and the stability against Ostwald ripening increased. The most stable nanoemulsions against Ostwald ripening could be prepared with emulsifiers of Tween 20 and Almax 3800 or lecithin in the ratio of 6:4 (wt%), which was verified with Ostwald ripening rate (${\omega}$). In addition, the emulsion stability by Turbiscan was observed to be consistent with results of changes in fat globule size with storage period.

• Journal of Microbiology
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• v.42 no.1
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• pp.60-63
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• 2004

A sucrose-agar globule (SAG) was newly introduced to increase production of the vesicular arbuscular mycorrhizal (VAM) fungal spores, Gigaspora gigantea and Glomus fasciculatum. An SAG inoculum and a sucrose-agar globule with root exudates (SAGE) inoculum were prepared, and their spore productions were compared with a soil inoculum. When the SAGE was used as the inoculum on sucrose-agar medium plates the number of spores was increased (35% more than the soil inoculum). After the soil inoculum and SAGE were inoculated on an experimental plant, Zingiber officinale, the percentage root colonization, number of VAM spores, and dry matter content were analyzed. It was observed that the SAGE showed a higher percentage of root colonization (about 10% more), and increases in the number of spores (about 26%) and dry matter (more than 13%) for the two VAM fungal spores than the soil inoculum. The results of this study suggested that the SAGE inoculum may be useful for the mass production of VAM fungi and also for the large scale production of VAM fungal fertilizer.