• Journal of KSNVE
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• v.2 no.4
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• pp.305-315
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• 1992

In order to investigate the effects of layer-wise in-plane displacements on vibration and damping characteristics of composite laminated plates, the finite element method based on the generalized laminated plate theory(GLPT) has been formulated. Specific damping capacity of each mode was obtained by modal strain energy method. To see the effect of transverse shear on deformation, the strain energy of stress components was computed. The accuracy of this study was examined for the cylindrical bending vibration of cross-ply plate strip. The results were very accurate compared with 3-D solutions. The numerical results show that through-thickness variation of in-plane displacements has not so much influence on the natural frequency, but has a great influence on the damping of composite plates, especially on the damping of thick composite plates since the damping is affected by local behavior while the natural frequency is affected by global behavior.

• Journal of Urban Science
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• v.12 no.2
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• pp.31-36
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• 2023

Facing the global crisis of climate change, waterfront areas are experiencing a significant increase in property damages and casualties. In light of the climate change era, this paper aims to identify resilience strategies against rising sea levels and associated natural disasters. A comparison and analysis of resilience plans and strategies have been conducted for five waterfront cities. In conclusion, three key points are suggested. Firstly, establishing partnerships locally and globally is an emerging trend to effectively and collaboratively address climate change. Secondly, resilience is a fundamental concept in designing social, built, and cyber infrastructure, requiring attention from stakeholders. Lastly, designing a well-operating system is critical to respond effectively when external shocks or stress occurs.

• Journal of Life Science
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• v.19 no.1
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• pp.21-33
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• 2009

In this study, we investigated the effect of Rhei Rhizoma (RR; 大黃) water extract on gene expression in a hypoxia model of cultured rat hippocampal neurons. RR water extract $(2.5{\mu}g/ml)$ was added to the culture media on day 10 in vitro (DIV10), and a hypoxic shock (2% $O_2$/5% $CO_2$, $37^{\circ}C$, 3 h) was given on DIV13. After maintaining the cultures in normoxia for 24 hr, total RNA was isolated and used for microarray analysis. The MA-plot indicated that most genes were up- or downregulated within 2-fold. There were more downregulated genes (725 ea) than upregulated ones (472 ea) when larger than Global M value 0.2 (i.e., >15% increase) or smaller than Global M value -0.2 (i.e., >15% decrease) were considered. Antiapoptosis genes such as Tegt (2.4-fold), Nfkb1 (2.4-fold) Veg (1.8-fold), Ngfr (1.6-fold) were upregulated, while pro-apoptosis genes such as Bad (-64%), Cstb (-66%) were downregulated. Genes for combating environmental stress (stress response genes) such as Defb3 (2.7-fold), Cygb (2.2-fold), Ahsg (2.18-fold), Alox5 (2-fold) were upregulated. Genes for cell proliferation (cell cycle-related genes) such as Erbb2 (1.84-fold), Mapk12 gene (1.8-fold) was upregulated. Therefore, RR water extracts upregulate many pro-survival genes while downregulating many pro-death genes. It is interpreted that these genes, in combination with other regulated genes, can promote neuronal survival in a stress such as hypoxia.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.12
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• pp.1734-1741
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• 2016

This study was conducted to examine the effects of adding phytoncide oil extracted from Korean pine nut cone by-product to the diet of dairy cows on milk yield and compositions, fatty acid characteristics, complete blood count and stress response. A total of 74 Holstein cows were used for 30 days and divided into two groups. Each group was given a basal diet (C) or an experimental diet containing phytoncide additives at 0.016% (T) in feed. The results showed that phytoncide feeding had no effect on milk yield. In addition, there were no observed effects on milk composition, but the ratio of fatty acid in milk was significantly affected by the phytoncide diet, and it showed a positive effect. Not only were the major functional fatty acids, conjugated linoleic acid and eicosapentaenoic acid increased, but also ${\omega}6:{\omega}3$ fatty acid ratio was reduced in milk of T group (p<0.05). In blood analysis, the complete blood count showed no significant difference between C and T group on all parameters. However, the cortisol concentration was significantly decreased in T group compared to control (p<0.05). Taken together, we suggest that phytoncide oil does not have a great influence on the physiological changes, but can be a potential feed additive that improves the milk fatty acid and stress resilience in dairy cows. In addition, it will contribute to the development of feed resource, a reduction in feed cost and a lessening of environmental pollution.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.127-127
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• 2017

Aluminum is the most abundant metallic element in the Earth's crust and considered as the most limiting factor for plant productivity in acidic soils. The inhibition of root growth is recognized as the primary effect of Al toxicity. Seeds of wheat cv. Keumkang (Korean cultivar) were germinated on petridish for 5 days and then transferred hydroponic apparatus which was treated with $0{\mu}M$ $AlCl_3$ (control), $100{\mu}M$ $AlCl_3$ and $150{\mu}M$ $AlCl_3$ for 5 days. The length of roots, shoots and fresh weight of wheat seedlings were decreased under aluminum stress. The concentrations of $K^+$, $Mg^{2+}$ and $Ac^{2+}$ were decreased whereas $Al^{3+}$ and $P_2O_5{^-}$ concentration was increased under aluminum stress. Using confocal microscopy, the fluorescence intensity of aluminum was increased with morin staining. In this study, a proteome analysis was performed to identify proteins, which is responsible to aluminum stress in wheat roots. In 10-day-old seedlings, proteins were extracted from roots and separated by 2-DE, stained by CBB. Using image analysis, a total of 47 differentially expressed protein spots were selected, whereas 19 protein spots were significantly up-regulated such as s-adenosylmethionine, oxalate oxidase, malate dehydrogenase, cysteine synthase, ascorbate peroxidase and 28 protein spots were significantly down-regulated such as heat shock protein 70, o-methytransferase 4, enolase, amylogenin by aluminum stress following protein spots analyzed by LTQ-FTICR mass spectrometry. The results provide the global picture of Al toxicity-induced alterations of protein profiles in wheat roots, and identify the Al toxicity-responsive proteins related to various biological processes that may provide some novel clues about plant Al tolerance.

• The Plant Pathology Journal
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• v.30 no.2
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• pp.220-227
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• 2014

The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were down-regulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P. chlororaphis O6 were generated in two GacS-regulated genes, trpE, encoding a protein involved in tryptophan synthesis, and prnA, required for conversion of tryptophan to the antimicrobial compound, pyrrolitrin. Failure of the trpE mutant to induce systemic resistance in tobacco against a foliar pathogen causing soft rot, Pectobacterium carotovorum SCCI, correlated with reduced colonization of root surfaces implying an inadequate supply of tryptophan to support growth. Although colonization was not affected by mutation in the prnA gene, induction of systemic resistance was reduced, suggesting that pyrrolnitrin was an activator of plant resistance as well as an antifungal agent. Study of mutants in the other GacS-regulated proteins will indicate further the features required for biocontrol-activity in this rhizobacterium.

• Computers and Concrete
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• v.6 no.4
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• pp.319-341
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• 2009

A new formulation based on lumped plasticity and inelastic hinges is presented in this paper for nonlinear analysis of Reinforced Concrete (RC) frame structures. Inelastic hinge behaviour is described using the principles of Continuum Damage Mechanics (CDM). Member formulation contains provisions to model stiffness degradation due to cracking of concrete and yielding of reinforcing steel. Depending on its nature, cracking is classified as concentrated or distributed. Concentrated cracking is accounted through a damage variable and its growth is defined based on strain energy principles. Presence of distributed flexural cracks in a member is taken care of by modelling it as non-prismatic. Plasticity theory supported by effective stress concept of CDM is applied to describe the post-yield response. Nonlinear quasi-static analysis is carried out on a RC column and a wide two-storey RC frame to verify the formulation. The column is subjected to constant axial load and monotonic lateral load while the frame is subjected to only lateral load. Computed results are compared with those due to experiments or other numerical methods to validate the performance of the formulation and also to highlight the contribution of distributed cracking on global response.

• Geomechanics and Engineering
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• v.19 no.2
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• pp.179-191
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• 2019

Based on the effective stress principle, a new formula for shear strength of unsaturated soil is derived under the general nonlinear Mohr-Coulomb (M-C) strength criterion to improve the classical strength criterion of unsaturated soil. Meanwhile, the simple irrigation model under steady seepage is adopted to obtain the distribution of the matrix suction or the degree of saturation (DOS) above the groundwater table in the slope. Then, combined with the improved strength criterion of unsaturated soil and the simple irrigation model under steady seepage, the limit equilibrium (LE) solutions for the unsaturated slope stability are established according to the global LE conditions of the entire sliding body with assumption of the stresses on the slip surface. Compared to the classical strength criterion of unsaturated soil, not only the cohesion soil but also the internal friction angle is affected by the matric suction or the DOS in the improved strength criterion. Moreover, the internal friction angle related to the matric suction has the nonlinear characteristics, particularly for a small of the matric suction. Thereafter, the feasibility of the present method is verified by comparison and analysis on some slope examples. Furthermore, stability charts are also drawn to quickly analyze the unsaturated slope stability.

• Bulletin of the Korean Chemical Society
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• v.32 no.6
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• pp.2051-2057
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• 2011

Several studies have demonstrated that nanoparticles (NPs) have toxic effects on cultured cell lines, yet there are no clear data describing the overall molecular changes induced by NPs currently in use for human applications. In this study, the in vitro cytotoxicity of three oxide NPs of around 100 nm size, namely, mesoporous silica (MCM-41), iron oxide ($Fe_2O_3$-NPs), and zinc oxide (ZnO-NPs), was evaluated in the human embryonic kidney cell line HEK293. Cell viability assays demonstrated that 100 ${\mu}g/mL$ MCM-41, 100 ${\mu}g/mL$ $Fe_2O_3$, and 12.5 ${\mu}g/mL$ ZnO exhibited 20% reductions in HEK293 cell viability in 24 hrs. DNA microarray analysis was performed on cells treated with these oxide NPs and further validated by real time PCR to understand cytotoxic changes occurring at the molecular level. Microarray analysis of NP-treated cells identified a number of up- and down-regulated genes that were found to be associated with inflammation, stress, and the cell death and defense response. At both the cellular and molecular levels, the toxicity was observed in the following order: ZnO-NPs > $Fe_2O_3$-NPs > MCM-41. In conclusion, our study provides important information regarding the toxicity of these three commonly used oxide NPs, which should be useful in future biomedical applications of these nanoparticles.

• Journal of the Korean Geosynthetics Society
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• v.19 no.4
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• pp.21-32
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• 2020

As the global large-scale infrastructure construction market expands, the construction of civil engineering structures in extreme environments such as cold or hot regions is being planned or constructed. Accordingly, the construction of the pile foundation is essential to secure the bearing capacity of the upper structure, but there is a concern about loss of stability and function of the pile foundation due to the possibility of ground deformation in extreme cold and hot regions. Therefore, in this study, a new type of pile foundation is developed to respond with the deformation of the ground, and the ground deformation that can occur in extreme cold and hot region is largely divided into heaving and settlement. The new type of pile foundation is a form in which a cylinder capable of shrinkage and expansion is inserted inside the steel pipe pile, and the effect of the cylinder during the heaving and settlement process was analyzed numerically. As a result of the numerical analysis, the ground heaving caused excessive tensile stress of the pile, and the expansion condition of the cylinder shared the tensile stress acting on the pile and reduced the axial stress acting on the pile. Ground settlement increased the compressive stress of the pile due to the occurrence of negative skin friction. The cylinder must be positioned below the neutral point and behave in shrinkage for optimum efficiency. However, the amount and location of shrinkage and expansion of cylinder must comply with the allowable displacement range of the upper structure. It is judged that the design needs to be considered.