• 한국한의학연구원논문집
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• 제14권1호
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• pp.113-116
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• 2008

Petiole explant of Glehnia littoralis Fr. Schmidt was in vitro cultured MS plant medium(DUCHEFA co.) supplemented with various plant growth regulators and examined to find out their optimum combination and concentration for plantlet regeneration. We investigated optimal condition for efficient plant regeneration through adventitious shoot formation on MS plant medium with various kinds of plant growth regulators. Embryogenic calli and adventitious shoot formation were greatly influenced by plant growth regulators. Embryogenic calli induction showed a good response on MS medium supplemented with NAA and BA than others. Especially, combination of 1.0 mg/L NAA and 0.5 mg/L BA on MS medium led to the greatest frequency in adventitious shoot. The results suggest that plant regulator selection be important factor to achieve an efficient regeneration.

• 한국한의학연구원논문집
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• 제14권3호
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• pp.49-56
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• 2008

Glehnia littoralis Fr. Schmidt et Miquel is an important medicinal plants in East Asian countries. This plant species naturally distributed in Korea, Japan, China, and Taiwan, but it is a rare plants living in the coastal dune in Korea. To investigate the genetic variation, genetic diversity and genetic evolutionary relationships of 14 different geographical G. littoralis, ITS sequence and random amplified polymorphic DNA (RAPD) were analyzed. On the basis of ITS sequences, it was clearly showed that the ITS1 and ITS2 sequences among 14 populations are identical regardless of geographical origin excepting 2 bp in pair-wise comparison of ITS1. Furthermore, RAPD results also showed that 14 different geographical G. littoralis produce various polymorphic patterns without critical relationship among neighboring regions. These combined results suggest that the geographical variation and genetic evolution of G. littoralis is stable and provide important information on genetic diversity, and conservation of this rare plant species in situ and ex situ.

• 한국약용작물학회지
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• 제17권2호
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• pp.139-144
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• 2009

This study was conducted to evaluate the vegetation, distribution and growth pattern of Glehnia littoralis community by the DCA ordination method in eastern coast of Korea. The number of plant species growth with G. littoralis in natural habitat was found 51 taxa, and species of high frequency among 51 taxa was Ixeris repens, Calystegia soldanella, Carex kobomugi, Elymus mollis, Lathyrus japonica and Zoysia macrostachya. Indicator species of Z. macrostachya, Linaria japonica, C. soldanella, I. repens, Carex pumila, Artemisia capillaris, Oenothera biennis, L. japonica and E. mollis in G. littoralis community was differential species of syntaxonomy. C. kobomugi, Z. macrostachya, E. mollis, Cynodon dactylon and L. japonica was the distribution in dryness sand and moisture sandyloam, I. repens, C. soldanella, L. japonica and C. pumila was the distribution in dryness sand. In coastal dune, I. repens, C. soldanella, Salsola komarovi and G. littoralis combined strength of the distribution, and sand of coast was typical of the habitat.

• 동의생리병리학회지
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• 제17권6호
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• pp.1519-1523
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• 2003

Glehnia littoralis Fr. Schmidt (Umbelliferae) is a short-lived herbaceous species that are mostly distributed throughout East Asia. Although G. littoralis has been regarded as ecologically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of Korean populations of this species. A high level of genetic variation was found in G. littoral is populations. Nine enzymes revealed 18 loci, of which 12 were polymorphic (66.7%). Genetic diversity at the species and population levels were 0.159 and 0.129, respectively. The sexual and asexual reproduction, high fecundity, and colonization process are proposed as possible factors contributing to genetic diversity. An indirect estimate of the number of migrants per generation (Nm = 1.45) indicated that gene flow was not extensive among Korean populations of this species. It is suggested that the ability of vegetation and artificial selection may have played roles in shaping the population structure of this species. we recommend that a desirable conservation population should be included at least 30 plants per population and especially those with high variation.

• 한국약용작물학회지
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• 제13권4호
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• pp.171-177
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• 2005

본 연구는 농업 자원식물의 남한 내 분포현황 및 유전자원 보존대책 확립의 일환으로 갯방풍의 자생지 생태 및 환경특성을 해안별로 조사한 바 이의 결과를 요약하면 다음과 같다. 1. 갯방풍의 분포는 갯방풍은 내륙해안과 도서지역의 해안 등 거의 전 지역의 대부분 사토에서 생육하고 있었다. 자생지는 만조선과 28.2m 거리, 해발고도 2.7m의 사구에 자생했으며, 해안별로는 동해안의 자생지가 서남해안에 비해 매우 넓고 밀도와 피도가 높았다. 2. 자생지의 혼생식물은 35여 종이었으며, 그 중 갯씀바귀, 좀보리사초, 통보리사초, 갯그령, 갯쇠보리, 띠 등의 밀도와 피도가 높았다. 3. 자생지의 연평균강수량 및 온도는 전체 평균이 각각 1250.3 mm, $12.5^{\circ}C$이었으며, 해안지역별로 보면 남해안이 연평균강수량 1427.3mm, $14.3^{\circ}C$로서 동해안의 1111.1 mm, $11.3^{\circ}C$보다 강수량이 많고 온도도 높은 편이었다. 4. 자생지의 토양의 화학적 특성은 pH가 전체평균 7.9로서 약 알카리를 띠고 있었으며, 유기물함량은 0.23%로 미약하였다. $P_2O_5$와 Ca 함량은 비교적 많았고, K은 극소량이었으며 Na이 상대적으로 적어 생육과 염분과의 상관관계가 크지 않는 것으로 보였다. 해안별로는 동해안이 서남해안과 다소의 차이를 보였다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 추계학술발표회
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• pp.241-242
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• 2008

• 생약학회지
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• 제28권1호
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• pp.1-8
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• 1997

Bangpoong (防風) is a popular crude drug used to expel wind from the body surface (祛風解表) to remove dampness (勝濕). And to relieve pain (正痛) and spasm (正痙). In China and Japan, roots of Saposhnikovia divaricata (Turcz.) Schischk. Is used as Bangpoong. However, the roots of Peucedanum japonicum Thunb. Or Glehnia littoralis (A. Gray) Fr. Schmidt ex Miquel, being called Sikbangpoong (植防風) and Wonbangpoong (元防風) respectively are used instead of Bangpoong in Korea. The ITS regions of nuclear ribosomal DNA were analyzed to determine original plants and to design a molecular identification method for the crude drugs used as Bangpoong in Korea and China. It is demonstrated that RFLP analysis via PCR has the great potential as a novel tool to test crude drugs for the quality control and standardization.