Genetic Diversity and Population Structure of Glehnia littoralis (Umbelliferae) in Korea

  • Huh Man Kyu (Department of Molecular Biology, Dongeui University) ;
  • Choi Joo Soo (Department of Molecular Biology, Dongeui University) ;
  • Huh Hong Wook (Department of Biology Education, Pusan National University) ;
  • Choi Yung Hyun (College of Oriental Medicine, Dongeui University) ;
  • Choi Byung Tae (College of Oriental Medicine, Dongeui University)
  • Published : 2003.12.01

Abstract

Glehnia littoralis Fr. Schmidt (Umbelliferae) is a short-lived herbaceous species that are mostly distributed throughout East Asia. Although G. littoralis has been regarded as ecologically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of Korean populations of this species. A high level of genetic variation was found in G. littoral is populations. Nine enzymes revealed 18 loci, of which 12 were polymorphic (66.7%). Genetic diversity at the species and population levels were 0.159 and 0.129, respectively. The sexual and asexual reproduction, high fecundity, and colonization process are proposed as possible factors contributing to genetic diversity. An indirect estimate of the number of migrants per generation (Nm = 1.45) indicated that gene flow was not extensive among Korean populations of this species. It is suggested that the ability of vegetation and artificial selection may have played roles in shaping the population structure of this species. we recommend that a desirable conservation population should be included at least 30 plants per population and especially those with high variation.

Keywords

References

  1. Flora of Korea Lee, Y.N.
  2. Ten Lectures of the Use of Medicinals Jiao, S.D.
  3. Can. J. For. Res. v.29 Genetic diversity and population structure of Korean alder (Alnus japonica Betulaceae). Huh, M.K.
  4. Am. Fern J. v.73 Starch gel electrophoresis of ferns: A compilation of grinding buffers, gel and electrode buffers, and staining schedules Soltis, D.E.;Haufler, C.H.;Darrow, D.C.;Gastony, G.J.
  5. Microsoft Windows-based Freeware for Population Genetic Analysis POPGENE version 1.31 Yeh, F.C.;Yang, R.C.;Boyle, T.
  6. New For. v.6 Factors influencing levels of genetic diversity in woody plant species Hamrick, J.L.;Godt, M.J.W.;Sherman-Broyles, S.L.
  7. Principles of Population Genetics(2nd) Hartl, D.L.;Clark, A.G.
  8. Evolution v.19 The interpretation of population structure by F-statistics with special regard to systems of mating Wright, S.
  9. J. Hered. v.86 GENEPOP version 1.2: a population genetics software for exact tests and ecumenicism Raymond, M.;Rouset, F.
  10. Marine Biol. v.112 Testing electrophoretic data for agreement with Hardy-Weinberg expectations Lessios, H.A.
  11. Proc. Natl. Acad. Sci. U.S.A. v.70 Analysis of gene diversity in subdivided populations Nei, M.
  12. Evolution v.38 Estimating F-statistics for the analysis of population structure Weir, B.S.;Cockerham, C.C.
  13. J. Hered. v.86 FSTAT v-1.2: a computer program to calculate F-statistics Goudet, J.
  14. Am. Nat. v.106 Genetic distance between populations Nei, M.
  15. Am. J. Hum. Genet. v.5 Some methods of estimating the inbreeding coefficient Li, C.C.;Horvitz, D.G.
  16. Evolution v.39 Rare alleles as indicators of gene flow Slatkin, M.
  17. Biostatistical Analysis(2nd) Zar, J.H.
  18. Syst. Zool. v.35 Multiple regression and correlation extensions of the Mantel test of matrix correspondence Smouse, P.E.;Long, J.C.;Sokal, R.R.
  19. Plant Population Genetics, Breeding and Genetic Resources Allozyme diversity in plant species Hamrick, J.L.;Godt, M.J.W.;Brown, A.H.D.(Eds.)
  20. Am. J. Bot. v.77 Local genetic and clonal structure in the tropical terrestrial bromeliad, Aechmea magdalenae Murawski, D.A.;Hamrick, J.L.
  21. Am. Sci. v.71 Clonal plant populations Cook, R.E.
  22. J. Ecol. v.73 The regulation of leaf, ramet, and genet densities in experimental populations of the rhizomatous perennial, Solidago canadensis Hartnett, D.C.;Bazzaz, F.A.
  23. Am. J. Bot. v.77 Patterns of clonal diversity in the Antennaria rosea (Asteraceae) polyploid agamic complex Bayer, R.J.
  24. Crop Sci. v.41 Genetic diversity and population structure of wild lentil tare Huh, M.K.;Huh, H.W.
  25. Conservation of Plant Genes Adams, R.P.;Adams, E.
  26. Ann. Rev. Ecol. Syst. v.15 Ecological determinants of genetic structure in plant populations Loveless, M.D.;Hamrick, J.L.