• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.108.3-109
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• 2003

In the present study, we investigated the antitumor effects of Ginsenoside Rh2 and $\beta$-glucan using an experimental metastatic mouse model intravenously injected with B 16 melanoma F10 cells. Oral administration to various concentration of $\beta$-glucan (50mg/kg, l00mg/kg and 200mg/kg) dose-dependently reduced the lung-metastatic potential of metastatic BI6 melanoma F10 cells in syngenic mice. At same dose, Ginsenoside Rh2(50mg/kg) has more antitumor effect than $\beta$-glucan(50mg/kg). (omitted)

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.244-252
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• 1998

Ginsenoside Rh, (G-Rh,) from Panax ginseng induced morphological features of apoptosis and DNA fragmentation as a biochemical marker of apoptosis confirmed by TUNEL reaction and agarose gel electrophoresis in human neuroblastoma SK-N-BE(2) and rat glioma C6Bu-1 cells During apoptosis by G-Rh2, protein kinase C (PKC) isoforms were analysed by immunoblotting. In SK-N-BE(2) cells, the levels of a, p and ${\gamma}$ subtypes were increased by undergoing apoptosis, while PKC e isoform increased early in treatment (3 h and 6 h). In addition, PKC s isoform gradually decreased during apoptosis by G-Rh2 and PKC $\theta$ isoform was detected in neither untreated- nor G-Rh1-treated SK-N-BE(2) cells (data not shown). However, no significant changes in the level of S and s isoforms were observed in C6Bu-1 cells undergoing apoptosis by G-Rh2. These results suggest that PKC subtypes may play differential roles in apoptotic signal pathways and their roles can be cell type-specific in apoptosis induced by G-Rh2.

• 한국식품과학회지
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• 제44권4호
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• pp.411-416
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• 2012

본 연구에서는 인삼의 수용성 물질의 추출 수율을 높이고 압출 온도가 ginsenoside 및 당의 변화에 미치는 영향을 조사하기 위하여 압출온도를 달리하여 제조한 압출성형 백삼의 추출수율과 성분의 변화를 조사하였다. 인삼의 증류수 추출 수율은 압출성형 백삼이 가장 높았으며 백삼이 가장 낮았다. 압출 성형백삼의 경우는 압출 온도가 높을수록 추출 수율이 증가하였다. 또한 증류수 추출시 80% 에탄올 추출시보다 추출수율이 증가하였다. 조사포닌 함량은 압출 성형 백삼이 가장 높았으며 이는 압출 온도가 증가함에 따라 조사포닌 함량도 증가하였다. 11종의 총 ginsenoside함량은 홍삼이 가장 높았다. 백삼에서는 Re의 함량이 가장 높았고, 홍삼에서는 Rg1, Rg3, Rb2가 가장 높았다. 압출성형 백삼에서는 Rg2, Rh1 및 Rh2의 함량이 증가되었다. 인삼의 유리당 함량은 홍삼이 가장 높았으며 압출 성형 인삼이 가장 낮았다. 인삼의 명도(L)값은 백삼이 가장 높았으며 압출 성형백삼이 가장 낮았다. 적색도(a)와 황색도(b) 값은 압출성형 백삼이 가장 높았다. 이상의 실험에서 압출 성형 백삼은 정수로 추출 시 추출 수율이 백삼에 비해 25%이상 높았고, 조사포닌 함량도 약 20% 높았다. 또한 Rg2, Rh1, Rh2, Rg3의 ginsenoside 함량이 백삼에 비해 월등히 높았다. 이는 압출 성형 백삼을 이용하여 제품 개발 시 높은 추출 수율, 사포닌 함량이 높은 제품을 만들 수 있을 가능성을 보여 주는 결과라 생각된다.

• Journal of Ginseng Research
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• 제41권3호
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• pp.330-338
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• 2017

Background: Ginsenoside Rh2 (G-Rh2) is a ginseng saponin that is widely investigated because of its remarkable antitumor activity. However, the molecular mechanism by which (20S) G-Rh2 triggers its functions and how target animals avoid its cytotoxic action remains largely unknown. Methods: Phage display was used to screen the human targets of (20S) G-Rh2. Fluorescence spectroscopy and UV-visible absorption spectroscopy were used to confirm the interaction of candidate target proteins and (20S) G-Rh2. Molecular docking was utilized to calculate the estimated free energy of binding and to structurally visualize their interactions. MTT assay and immunoblotting were used to assess whether human serum albumin (HSA), bovine serum albumin (BSA), and bovine serum can reduce the cytotoxic activity of (20S) G-Rh2 in HepG2 cells. Results: In phage display, (20S) G-Rh2-beads and (20R) G-Rh2-beads were combined with numerous kinds of phages, and a total of 111 different human complementary DNAs (cDNA) were identified, including HSA which had the highest rate. The binding constant and number of binding site in the interaction between (20S)-Rh2 and HSA were $3.5{\times}10^5M^{-1}$ and 1, and those in the interaction between (20S) G-Rh2 and BSA were $1.4{\times}10^5M^{-1}$ and 1. The quenching mechanism is static quenching. HSA, BSA and bovine serum significantly reduced the proapoptotic effect of (20S) G-Rh2. Conclusion: HSA and BSA interact with (20S) G-Rh2. Serum inhibited the activity of (20S) G-Rh2 mainly due to the interaction between (20S) G-Rh2 and serum albumin (SA). This study proposes that HSA may enhance (20S) G-Rh2 water solubility, and thus might be used as nanoparticles in the (20S) G-Rh2 delivery process.

• 한국식품영양과학회지
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• 제39권11호
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• pp.1660-1665
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• 2010

홍삼 파우치 제품의 성분 및 제조방법의 표준화를 위한 일환으로 국내 유통 중인 홍삼 파우치의 사포닌 함량 및 이화학적 특성을 분석하였다. 총 ginsenoside 함량은 5.5~185.7 mg/100 mL의 함량을 나타내었고 항암효과로 알려진 ginsenoside $Rg_3$, $Rg_2$, $Rh_1$과 $Rh_2$의 함량 분포는 $Rg_3$는 1.6~46.3 mg/100 mL, $Rg_2$는 미검출~22.0 mg/100 mL, $Rh_1$은 미검출~4.3 mg/100 mL, $Rh_2$는 미검출~20.4 mg/100 mL의 분포를 나타내었다. 항 당뇨 효과가 있는 ginsenoside $Rb_2$와 Re의 함량분포는 $Rb_2$는 미검출~10.8 mg/100 mL, Re는 미검출~7.0 mg/100 mL의 함량을 나타내었으며 그 밖의 사포닌 중 ginsenoside $Rb_1$는 미검출~25.2 mg/100 mL, Rc는 미검출~12.5 mg/100 mL, Rd는 미검출~11.3 mg/100 mL, Rf는 미검출~5.9 mg/100 mL, $Rg_1$는 미검출~4.4 mg/100 mL까지의 함량 분포를 보였다. 이화학적 특성 분석결과 총당함량은 226.6~3,102.9 mg/100 mL, 당도는 $1.4\sim9.5^{\circ}Bx$, 탁도는 82.2~100.0%, pH는 4.1~5.0의 범위를 나타내었다. 국내에서 수집한 홍삼 파우치 제품의 약 50%(21~24개 제품)가 ginsenoside $Rb_1$, $Rb_2$, Rc, Rd, Re, $Rg_1$이 미검출 되었으며, 각 제품별 사포닌 함량 차이가 매우 크게 나타나, 국내유통 중인 홍삼 제품에 대한 기준 설정 및 생산방법의 표준화가 필요할 것으로 사료된다.

• 약학회지
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• 제60권2호
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• pp.58-63
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• 2016

The purpose of this study is to develop a new preparation process of ginseng berry extracts having high concentrations of ginsenoside Rh1, Rg2, Rg5, F4, a special component of red and black ginseng. Chemical transformation from ginseng saponin glycosides to prosapogenin was analyzed by the HPLC. Extracts of ginseng (Panax ginseng) berry was processed under several treatment conditions including ultrasonication treatments. The content of total saponin reached their heights at 6 hr (UGB-6, 61.760%) of ultrasonication treatment, followed by 10 hr (UGB-10, 53.009%) and 9 hr (UGB-9, 50.652%) of ultrasonication treatment at $100^{\circ}C$. Results of those treatments showed that the quantity of ginsenoside Rh1 increased by over 15% at 10 hr of ultrasonication treatment at $100^{\circ}C$. The results of processing with UGB-10 indicate that the ultrasonication processed ginseng berry extracts that had gone through 10 hr treatments were found to contain the largest amount of ginsenoside Rh1 (15.358%), Rg2 (6.301%), Re (4.567%) and F4 (2.658%). In addition, UGB-6 contained ginsenoside Rg3 (13.632%) at high concentrations. It is thought that such results provide basic information in preparing ginseng berry extracts with functionality enhanced.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1817-1821
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• 2016

Objectives: To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells. Materials and Methods: SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting. Results: It was found that the proliferation of SP was significantly faster than that of NSP (P<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (P<0.05). Conclusions: With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.

• 한국자원식물학회지
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• 제19권1호
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• pp.139-143
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• 2006

고기능성 홍삼사포닌성분의 함량을 증대시키기 위한 목적으로 홍삼엑스에 열처리, 산(acid)처리하여 그 가능성을 조사하였다. 산도를 조정하지 않은 무처리구(control, pH 4.4)에 $120^{\circ}C$ 열처리한 경우 ginsenoside-$Rg_3$의 함량이 약 2배 정도 증가였다. 구연산으로 pH 2.0으로 조정하고 온도처리한 처리구에서는 2.8배나 많은 ginsenoside-$Rg_3$ 성분이 증가하였으나 다른 유효한 사포닌의 파괴가 두드러져 처음 홍삼엑스에 함유되어 있던 총사포닌의 65% 정도가 소실되었다. $80^{\circ}C$에서 12시간 처리를 한 경우에는 pH를 2.5와 2.0로 조정한 처리구에서는 11.20 mg과 12.50 mg으로 홍삼엑스의 3.3 mg보다 3.3배 이상 ginsenoside-$Rg_3$ 성분이 변환되었다. Ginsenoside-$Rb_1,\;Rb_2$, Rc, Re, $Rg_1$의 함량이 산도가 높아짐에 따라서 급격히 소실되었고 홍삼 특이성분(ginsenoside-$Rg_3,\;Rh_2,\;Rh_1$)의 함량은 현저히 증가되었다. 매실엑스로 pH를 2.5로 조정한 처리구에서는 13.34 mg으로 홍삼엑스의 3.3 mg보다 4배 이상 변환된 것으로 분석되었다. 비록 31%정도의 total saponin의 감소가 있었으나 $120^{\circ}C$의 고온처리에서 처럼 다른 유효한 사포닌의 큰 손실 없이 $60^{\circ}C$에 12시간 처리하는 것만으로도 다량의 ginsenoside-$Rg_3$를 생산하는 것을 확인하였다.

• 한국약용작물학회지
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• 제20권4호
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• pp.270-276
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• 2012

This study was performed to enhance contents of low molecular weight ginsenoside Rh2 and Rg3 using an ultra high pressure and steaming process in wild cultured-Root in wild ginseng. For selective increase in contents of Rg3 and Rh2 in cultured wild ginseng roots, an ultra high extraction was applied at 500MPa for 20 min which was followed by steaming process at $90^{\circ}C$ for 12 hr. It was revealed that contents of ginsenosides, Rb1, Rb2, Rc and Rd, were decreased with the complex process described above, whereas contents of ginsenoside Rh2 and Rg3 were increased up to 4.918 mg/g and 6.115 mg/g, respectively. In addition, concentration of benzo[${\alpha}$]pyrene in extracts of the cultured wild ginseng roots treated by the complex process was 0.64 ppm but it was 0.78 ppm when it was treated with the steaming process. From the results, it was strongly suggested that low molecular weight ginsenosides, Rh2 and Rg3, are converted from Rb1, Rb2, Rc, and Rd which are easily broken down by an ultra high pressure and steaming process. This results indicate that an ultra high pressure and steaming process can selectively increase in contents of Rg3 and Rh2 in cultured wild ginseng roots and this process might enhance the utilization and values of cultured wild ginseng roots.

• Preventive Nutrition and Food Science
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• 제14권3호
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• pp.201-207
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• 2009

In an effort to improve ginsenoside bioavailability, the ginsenosides of fermented red ginseng were examined with respect to bioavailability and physiological activity. The results showed that the fermented red ginseng (FRG) had a high level of ginsenoside metabolites. The total ginsenoside contents in non-fermented red ginseng (NFRG) and FRG were 35715.2 ${\mu}g$/mL and 34822.9 ${\mu}g$/mL, respectively. However, RFG had a higher content (14914.3 ${\mu}g$/mL) of ginsenoside metabolites (Rg3, Rg5, Rk1, CK, Rh1, F2, and Rg2) compared to NFRG (5697.9 ${\mu}g$/mL). The skin permeability of RFG was higher than that of NFRG using Franz diffusion cells. Particularly, after 5 hr, the skin permeability of RFG was significantly (p<0.05) higher than that of NFRG. Using everted instestinal sacs of rats, RFG showed a high transport level (10.3 mg of polyphenols/g sac) compared to NFRG (6.67 of mg of polyphenols/g sac) after 1 hr. After oral administration of NFRG and FRG to rats, serum concentrations were determined by HPLC. Peak concentrations of Rk1, Rh1, Rc, and Rg5 were approximately 1.64, 2.35, 1.13, and 1.25-fold higher, respectively, for FRG than for NFRG. Furthermore, Rk1, Rh1, and Rg5 increased more rapidly in the blood by the oral administration of FRG versus NFRG. FRG had dramatically improved bioavailability compared to NFRG as indicated by skin permeation, intestinal permeability, and ginsenoside levels in the blood. The significantly greater bioavailability of FRG may have been due to the transformation of its ginsenosides by fermentation to more easily absorbable forms (ginsenoside metabolites).