• 한국융합학회논문지
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• 제8권8호
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• pp.191-196
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• 2017

본 연구는 파인 버블수 처리시 인삼 종묘삼 생장에 미치는 영향 대해서 조사하는 융합연구로써 고년 근 인삼 재배 시 적용 가능성을 높이기 위한 기초자료를 제공하고자 실시하였다. 연구결과 일반수와 파인버블수를 각각 처리한 종묘삼이 성장함에 따라 지상부와 지하부의 부위별 차이점이 나타났다. 지상부에서는 파인버블수를 처리한 종묘삼이 일반수를 처리한 종묘삼보다 잎의 성장이 약 10%증가 하였고 줄기는 약 6% 성장이 일어났다. 지하부에서는 뿌리길이는 약 5% 증가하고 주근 폭이 약 8% 증가되었고 뿌리 무게는 생중량이 약 9%, 건중량은 약 7%의 무게 증가가 일어났다. 이것은 뿌리전체만 성장한 것이 아니라 주근의 생성이 빨리 되면서 전체적으로 뿌리성장이 약 7% 증가하였다고 판단된다. 이와 같은 결과는 종묘삼의 생육증가가 파인버블수의 특이한 물질적 성질이 종묘삼의 성장에 자극 주었다고 볼 수 있다. 따라서 본 연구의 결과는 종묘삼을 이용한 초기 연구로써 향후 고년 근 인삼재배에 적용 가능성이 높다고 판단되며 이후 2년 근 인삼재배에서 적용한 연구와 토양조건에 따른 용존 산소량을 조절함으로써 최적 재배조건을 찾아가는 추가 연구가 필요할 것으로 생각된다.

• 한국약용작물학회지
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• 제22권5호
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• pp.391-397
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• 2014

To control the disease of root rot in ginseng nursery, inorganic sulfur solution of 0.1%, 1.0%, and 2.0% were irrigated by amount of $10{\ell}$ per $3.3m^2$ before sowing. On the last ten days of July, Fusarium solani and F. oxysporum were similarly detected by 44.8% and 43.8%, respectively, while Cylindrocarpon destructans was low detected by 4.4% in the diseased seedling. The more sulfur's concentration was increased, the more soil pH was decreased. Soil pH was decreased from 5.87 to 4.59 by the irrigation of sulfur solution of 1.0%. The more sulfur's concentration was increased, the more electrical conductivity (EC) of soil was increased. EC was increased from 0.27 dS/m to 1.28 dS/m by the irrigation of sulfur solution of 1.0%. Irrigation of sulfur solution was effective on the inhibition of damping-off caused by Rhizoctonia solani in ginseng seedling. Control value for damping-off by the irrigation of sulfur solution of 1.0% and 2.0% were 75.7%, and 78.5%, respectively. Growth of leaf was inhibited by the irrigation of sulfur solution of 2.0%. Root weight per $3.3m^2$ showed the peak in sulfur solution of 1.0%, while survived-root ratio and root weight per plant were decreased in the level of 2.0%. Survived-root ratio of seedling in sulfur solution of 1.0% was distinctly increased by 4.7 times compare to the control, but control value for root rot was relatively low as 49.2%. Mycelium growth of C. destructans, F. solani, and R. solani were distinctly inhibited by the increase of sulfur's concentration in vitro culture using PDA medium.

• Journal of Ginseng Research
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• 제43권1호
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• pp.38-48
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• 2019

Background: Interspecific ginseng hybrid, Panax ginseng ${\times}$ Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F1 progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid. Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with $GA_3$ to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS. Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L $GA_3$ for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The $F_1$ hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides $F_{11}$ in P. quinquefolius), and higher amount of other ginsenosides than its parents. Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants.

• Mycobiology
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• 제42권1호
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• pp.12-16
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• 2014

We isolated and examined a new Alternaria sp., which causes leaf spots on Peucedanum japonicum in Korea, by using molecular and morphological methods. Phylogenetic analysis based on a combined internal transcribed spacer region analysis and two protein-coding genes (gpd and Alt a1) demonstrated that the causal fungus was most closely related to A. cinerariae and A. sonchi, and relevant to A. brassicae. However, conidial morphology indicated that it is a novel species within the genus Alternaria, and therefore we have assigned the fungus a new name in this study.

• 한국연초학회지
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• 제18권1호
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• pp.5-11
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• 1996

Field experiment was conducted to get the basic information about the difference in color and plastid pigments at the different stage of harvest of flue-cured tobacco cultivated in paddy field, and compared to upland ones. All tested crops were primed in lots of 3 to 4 at each priming, beginning at the bottom of the plant. Leaf pigments and chromatic values of tobacco leaves were determined in samples collected at weekly intervals, and according to analyses obtained from 4 stalk positions. By the degree of maturity, leaves harvested were separated with visual characters into four classes of immature, mature, ripe and mellow. regardless of stalk position, chlorophyll contents was higher in the order of immature > mature > ripe > mellow, respectively. In fresh leaves, chlorophyll levels was higher with ascending stalk position. chlorophyll a and b in cured leaves were less than 10% of the amount present at harvest, and it was estimated that maturity condition also influenced the rates of degradation of plastid pigments. Fully mature leaves of upland diverted from paddy field showed slightly higher in chlorophyll contents than in those of upland tobacco ones but somewhat lowered in $L^*$ and $b^*$ values, and there was no difference in $a^*$ value.

• 한국연초학회지
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• 제16권1호
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• pp.53-56
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• 1994

This study was aimed at finding out the effect of herbicide quinclorac (3, 7-dichloro-8- quinoline carbxylic acid) on tobacco culture in paddy soil. Herbicides quinclorac(Pozol, Pulta and Tomata) was randomly applied to subdivided paddy soil in Sep. 1992, and tobacco seedlings were transplanted to the field when seedling averaged 2cm in height in April 1993. Experiment plots were divided into non - treatment, standard(3kg/10a) and two times(6kg/10a) in amounts for each three herbicides. The symptoms of damage from herbicide were sighted when the plants had developed 8-9 leaves at 30 day after transplanting in standard amount plots, and when the plants had developed 4-5 leaves at 20 days after transplanting in two times treatments. The new leaves from damaged plants were bended out, and leaf color changed from green to dark - green, and then gradually advanced to abnormal narrow leaves. Standard treatments of herbicides showed a decrease of 18% in price per kg, 18% in yield and 33% in value per 10a than those of non - treatment, while two times treatments showed a decrease of 33%, 29%, 52% compared with non - treatment, respectively.

• Journal of Ginseng Research
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• 제27권1호
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• pp.32-36
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• 2003

본 시험은 인삼비료인 약토를 대체 하기 위하여 인삼비료인 약토 외에 4종의 퇴비를 공시하여 약토 대체 가능성을 검토하였다. 퇴비종류별 식부가능묘삼의 생산량은 약토>PL퇴비)팽이버섯퇴비 >볏짚우분퇴비>HJ퇴비 순으로 약토가 가장 우수하였다. 퇴비종류별 묘삼중의 무기성분 함량은 약토구는 타 퇴비구에 이해 잎과 줄기에서 칼슘함량이 높았으나 뿌리에서는 차이가 없었으며, PL퇴비구는 잎과 줄기에서 질소함량이 다소 높게 나타났다. HJ퇴비는 잎에서 인산과 카리의 함량이 높았고, 경에서 인산이, 뿌리에서 질소의 함량이 높았고, 팽이버섯퇴비는 잎과 줄기에서 질소와 마그네슘함량이 높았고, 뿌리에서는 마그네슘함량이 높았다. 볏짚우분퇴비는 줄기에서 질소, 카리, 마그네슘 함량이 높았고, 뿌리에서 인산, 카리 함량이 높았다. 우량 양직묘 생산을 위해서는 완숙된 퇴비를 사용하여야 하며, PL퇴비와 팽이버섯퇴비는 약토 다음으로 식부가능 묘삼 생산량이 많아, 완숙시킨 후 재검토가 필요하다.

• 한국토양비료학회지
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• 제18권1호
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• pp.32-37
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• 1985

볏짚부초의 인삼(人蔘) 근부(根部) 및 지상부생육(地上部生育)에 대(對)한 효과(效果)와 토양(土壤)의 물리(物理) 및 화학성(化學性)에 주는 영향(影響)은 포장조건(圃場條件)에서 조사(調査)하였다. 볏짚부초는 수량(收量), 경장(莖長)과 엽면적(葉面積)을 증대(增大)하고 낙엽주율과 결근율을 감소시켰다. 볏짚부초는 토양(土壤)의 경도(硬度), 공극율(孔隙率)을 좋게 유지시켰다. 볏짚부초는 보수력(保水力)을 증대(增大)시키고 지온(地溫)을 강하(降下)시켰으며 후행(後行), 전행(前行) 중간행(中間行)의 순(順)으로 그 효과가 적어졌다. 볏짚부초는 토양의 유효인산(포장에 따라 극심하게), 치환성 칼리, 유기물 함량을 증대시키고 치환성 칼슘함량을 감소시키고 pH 강하를 억제하였다. 볏짚부초는 잡초발생(雜草發生)을 크게 억제하였고 토니(土泥)의 오염을 막았으며, 골가토, 두둑다짐, 상면가토 등의 작업을 배제하였다.

• 한국약용작물학회지
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• 제20권4호
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• pp.217-221
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• 2012

This research was conducted to investigate the influence of various organic substrates on growth and yield of ginseng seedling grown organically in the closed plastic house. The pH and EC of substrates used for organically ginseng cultivation ranged 5.93~6.78 and 0.03~0.15 dS/m respectively. The concentrations $NH_4$-N and $NO_3$-N respectively was 14.01~68.63 mg/L, 5.60~58.83 mg/L. The average quantum of the closed plastic house was range from 10 to 16% of natural light. In July and August, the maximum temperature of the closed plastic house did not exceed 30 and the average temperature was maintained within 25 lower than the field because air conditioning ran. The PPV-1 and PPV-2 bed soil substrates produced higher stem length, stem diameter, shoot fresh weight and leaf area than those of conventional culture. In PPV-2 bed soil substrates, root fresh weight and root diameter was the highest. The root fresh weight of PPV-2 bed soil substrates in closed plastic house was maximum 25% heavier than the conventional cultivation. The results of this experiment will be utilized for making new substrate application for organic ginseng culture in the plastic house.

• 한국산림과학회지
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• 제99권6호
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• pp.863-870
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• 2010

산지에서 재배되는 산양삼의 생육특성을 알아보기 위하여 재배지 내에 $20{\times}20$ m 방형구를 설치하고 설치된 지역의 입지 및 토양 특성을 조사하였다. 입지특성에 관해서는 상층을 위주로 매목조사를 실시하였다. 임관울폐도는 균일하게 80%로 조절하고, 조사지내의 시험구는 미세기후를 측정하기 위한 Hobo를 설치하여 재배지의 기온, 토양내 온도변화 등을 측정하고 지역적 특성을 조사하였다. 시험구의 입지특성은 모든 조사구에서 다양하게 나타나고 있다. 또한 시험구내의 토양 특성은 우리나라의 전형적인 산림토양의 특성을 나타내고 있다. 특히 4월경 지상부가 출현하는 시기에는 $15^{\circ}C$이상의 적온이 필요한 것으로 나타났다. 지중온도가 상승하여 $8^{\circ}C$에 도달하면, 산양삼의 정단부에서 출엽을 시작하며, 지중온도가 $18^{\circ}C$를 넘어서면 낙엽이 지기 시작한다. 묘삼 식재의 경우 초기 출아율이 높으나 3년이후 급격히 출아율이 감소하여 자연도태가 이루어지고, 종자 파종의 경우에는 초기 출아율이 묘삼식재보다는 낮으나 자연도태율이 묘삼식재보다 낮은 것으로 나타났다.