• Journal of Ginseng Research
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• 제46권6호
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• pp.790-800
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• 2022

Background: Panax ginseng Meyer is one of the most valuable medicinal plants which is enriched in anti-microbe secondary metabolites and widely used in traditional medicine. Botrytis cinerea is a necrotrophic fungus that causes gray mold disease in a broad range of hosts. B. cinerea could overcome the ginseng defense and cause serious leaf and root diseases with unknown mechanism. Methods: We conducted simultaneous transcriptomic and metabolomic analysis of the host to investigate the defense response of ginseng affected by B. cinerea. The gene deletion and replacement were then performed to study the pathogenic gene in B. cinerea during ginseng - fungi interaction. Results: Upon B. cinerea infection, ginseng defense responses were switched from the activation to repression, thus the expression of many defense genes decreased and the biosynthesis of antifungal metabolites were reduced. Particularly, ginseng metabolites like kaempferol, quercetin and luteolin which could inhibit fungi growth were decreased after B. cinerea infection. B. cinerea quercetin dioxygenase (Qdo) involved in catalyzing flavonoids degradation and ∆BcQdo mutants showed increased substrates accumulation and reduced disease development. Conclusion: This work indicates the flavonoids play a role in ginseng defense and BcQdo involves in B. cinerea virulence towards the P. ginseng. B. cinerea promotes disease development in ginseng by suppressing of defense related genes expression and reduction of antifungal metabolites biosynthesis.

• 한국연초학회지
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• 제4권2호
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• pp.51-56
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• 1982

An automatic method for the determination of total amino acid in tobacco was studied. This method makes use of the reaction of 2, 4, 6-Trinitrobenzene sulfonate with amino acid. Results obtained with this simple and rapid method agreed with those obtained with the offical method.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1988년도 춘계 학술대회지
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• pp.90-91
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• 1988

• 한국작물학회지
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• 제34권4호
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• pp.422-427
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• 1989

제초제 2, 4-D의 처리가 인삼의 생육 및 근수량에 미치는 영향을 구명하기 위하여 2, 4-D 제초제량 (유제 :70ml/100$\ell$/10a, 수화제 : 250g/100$\ell$/10a)의 0.5, 1.0, 2.0 배액을 2년, 3년 및 4년생 인삼에 대하여 출아후 40일에 각각 엽면처리아여 경엽의 상육과 장과의 착생 및 근수량의 변이를 조사하고 무처리대조구와 비교 분석하였던 바 그 결과를 요약하면 다음과 같다. 1. 2년, 3년 및 4년생 인삼에 2, 4-D를 일반제초약량의 2배로 경엽산포하여도 지상부의 전체적인 생육상은 무처리구와 차이가 없었으며 특별한 약해현장도 나타나지 않았다. 2. 2년, 3년 및 4년생 인삼에 있어 무처리구와 2, 4-D 유제 및 수화제 처리 농도간에 엽장, 여폭, 경장 및 경직경 등 유의차가 인정되지 않았다. 3. 3년생 및 4년생 인삼의 장과착생수는 2, 4-D 유제를 일반체초제의 2배로 처리한 구에서도 무처리구와의 유의차는 인정되지 않았으며 외관상의 형태에도 전혀 이상이 없었다. 4. 4년생 인삼에 있어 무처리구와 2, 4-D 유제 및 수화제의 처리농도간에 근장, 근식경, 지근수 및 근주의 차이는 인정되지 않았다. 5. 묘삼에 2, 4-D 유제 및 수화제의 일반제초제량을 경엽처리할 경우 줄기가 연화되어 구부러졌으며 특히 수화제 처리시는 잎의 끝이 백화고사되는 약해현장을 나타내었고 구부러진 줄기는 2~3일 후 다시 상향으로 회복되기는 하였으나 이상신장된 결과를 보였다.

• 한국작물학회지
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• 제49권2호
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• pp.94-99
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• 2004

This study was conducted to determine the effect of salinity on the growth and development of Korean ginseng (Panax ginseng C.A.Meyer) and to evaluate the inorganic ion content in Korean ginseng with different general complete fertilizer (GCF) and NaCI concentrations at two growth stages. The stem height of Korean ginseng treated with different GCF and NaCI concentrations decreased at the higher EC (2.0 dS m$^{-1}$ ), but there were no significant difference in the stem diameter, the leaf length, and the leaf width among different treatments. The root growth increased with the supply of GCF. Especially, the root growth was facilitated two times at 3.0 dS $\textrm{m}^{-1}$ as compared to control. But the root growth more sharply decreased with NaCI treatment than GCF. The $\textrm{K}^{+}$ and $\textrm{Mg}^{2+}$ content in leaves and roots increased with GCF at the early growth stage. At the late growth stage, the $\textrm{K}^{+}$ content in leaves decreased but the $\textrm{Ca}^{2+}$ and $\textrm{Mg}^{2+}$ content increased. The $\textrm{Ca}^{2+}$ and $\textrm{Mg}^{2+}$ content in roots increased but the $\textrm{K}^{+}$ content decreased. The $\textrm{Na}^{+}$ content in Korean ginseng increased sharply with NaCl treatment. The $\textrm{NO}_3^{-}$ content in leaves and $\textrm{NH}_4^{+}$ content in leaves and roots increased as GCF concentration increased. The $\textrm{NO}_3^{-}$ content in leaves, stems, and roots at the late growth stage decreased as NaCl concentration increased. The $\textrm{NH}_4^{+}$ content in leaves and roots decreased significantly at the early growth stage, but it decreased significantly in leaves and stems at the late growth stage. The root activity of Korean ginseng increased with GCF, but decreased as the EC increased with NaCl. The water potential of leaves with GCF showed no significant difference compare to control, but the water potential of leaves treated with NaCl decreased as EC increased.

• Journal of Ginseng Research
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• 제36권2호
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• pp.211-217
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• 2012

Endophytic fungi were isolated from various tissues (root, stem, petiole, leaf, and flower stalk) of 3- and 4-year-old ginseng plants (Panax ginseng Meyer) cultivated in Korea. The isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer (ITS), 1-5.8-ITS 2. A morphological characterization was also conducted using microscopic observations. According to the identification, 127 fungal isolates were assigned to 27 taxa. The genera of Phoma, Alternaria and Colletotrichum were the most frequent isolates, followed by Fusarium, Entrophospora and Xylaria. Although 19 of the 27 taxa were identified at the species level, the remainder were classified at the genus level (6 isolates), phylum level (Ascomycota, 1 isolate), and unknown fungal species (1 isolate). Endophytic fungi of 13 and 19 species were isolated from 3- and 4-year-old ginseng plants, respectively, and Phoma radicina and Fusarium solani were the most frequently isolated species colonizing the tissues of the 3- and 4-year-old ginseng plants, respectively. The colonization frequency (CF%) was dependant on the age and tissue examined: the CFs of the roots and stems in the 3-year-old ginseng were higher than the CF of tissues in the 4-year-old plants. In contrast, higher CFs were observed in the leaves and petioles of 4-year-old plants, and endophytic fungi in the flower stalks were only detected in the 4-year-old plants. In conclusion, we detected diverse endophytic fungi in ginseng plants, which were distributed differently depending on the age and tissue examined.

• Journal of Ginseng Research
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• 제14권1호
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• pp.57-62
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• 1990

In order to Investigate the mechanism of the leaf-burning disease of ginseng (Panax ginseng C.A. Meyer), studies on the generation of singlet oxygen (1O2) and the photooxidation of the pigments were carried out in comparison with the ones of soybean (G1ycine max L). The studies were mainly focalized on the effects of light intensity, light intensity, inhibitor and electron donor/acceptor of the Photosynthetic electron transport system. When we measured the amounts of 1O2 generated in the thylakoids of ginseng and soybean by the irradiation of light (300 w/m2) as a function its time. It was identified that a higher amount of 1O2 was formed in the ginseng thylakoid than the case of soybean. A generation ratio of lO2 between ginseng and soybean sltbstantially identical in the range of light intensities 50∼150w/m2 However much higher amount of 1O2 was generated in ginseng by irradiation of strong intensity of light (200 500w/m2). Wave length dependency on the generation of 1O2 and the pigment photooxidation was observed on ginseng thylakoids; red light (600-700 nm) gave a maximum effect in the contrast with blur green light (400-60 nm). When the ginseng thylalioid was treated with the electron donor (Mn2+) and acceptors (DCPIP, FeCy) of the photosynthetic electron transport system. a drastic inhibition of 1O2 generation was observed. However, treatment with its inhibitors (DCMU, KCW) activated 1O2 generation. An interesting fact that an electron donor or acceptor of the photosystem II(P680) Inhibited 1O2 generation, suggests an intimate relationship between 1O2 generation and photosystem II.

• 동의생리병리학회지
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• 제22권2호
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• pp.397-402
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• 2008

The purpose of this study is to prepare black Panax ginseng leaf (PGL) and evaluate its antioxidative effect. In order to make black PGL, the raw PGL was successiely steamed at $95^{\circ}C$ for 3 hr nine times. The antioxidant activities of total saponins (Sa) from PGL and black PGL against peroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. Specific TOSC values for black PGL-Sa against peroxyl radicals and peroxynitrites were 2.3-fold and 2.1-fold of PGL-Sa, respectively, and 2.2-fold and 5.2-fold of glutathione, a positive control antioxidant, respectively. The black PGL-Sa exhibited stronger antioxidative effect than PGL-Sa. The main ginsenosides of black PGL were $Rg_3,\;Rk_1\;and\;Rg_5$. Among the saponins in black PGL, the amount of ginsenoside $Rg_3$ was examined by HPLC. 22.12 mg of ginsenoside $Rg_3$ was obtained from 1g of dried black PGL.

• 한국응용곤충학회지
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• 제33권4호
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• pp.237-241
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• 1994

1984년부터 1993년까지 해충 피해가 나타난 66개 인삼 포장에서 해충의 종류와 피해 양상을 조사하였다. 곤충유로 큰검정풍뎅이, 참검정풍뎅이, 큰다식풍뎅이, 애우단풍뎅이, 방아벌레, 땅강아지, 왕귀뚜라미, 조명나방, 숫검정밤나방, 도둑나방, 벼잎물가파리, 미동정 줄기굴파리류, 가루깍지벌레, 달팽이류도 들민달팽이와 명주달팽이 그리고 쥐류 및 꿩이 인삼을 가해하는 것으로 확인되었다. 이 중 큰검정풍뎅이, 참검정풍뎅이, 땅강아지, 들민달팽이, 명주달팽이의 발생빈도가 높은 편이었다. 인산의 지하부(뿌리) 피해는 경사지의 2년근 포장에서 춘기 (5~6월)과 추기(9~10월)에 나타나고 지상부(잎과 줄기) 피해는 평지의 3년근 이상의 부초 포장에서 춘기에 주로 나타났다. 3개 포장이 심한 지하부 피해로 인해 폐장되었다.

• Journal of Ginseng Research
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• 제11권1호
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• pp.39-45
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• 1987

Panu ginseng (6 Year old) was grown $17^{\circ}C$/$15^{\circ}C$ and $27^{\circ}C$ day/$23^{\circ}C$ night in the light room of phytotron for 84 days. The composition of neutral lipid(NL), glycolipid(GL), phospholipid(PL) and fatty acids were investigated in leaves. The contents of NL and GL were higher in $25^{\circ}C$ while PL was lower. Similarity (simple correlation) of lipid composition between $16^{\circ}C$ and $25^{\circ}C$ was not significant for PL and GL but significant for NL(p = 0.001), indicating that PL and GL were important factors in the mostability. Similarity of fatty acid composition between growth temperatures was highly significant (p = 0.001) for all three lipids, while similarity between lipids was significant between NL and PL (p=0.01) and NL and GL (p=0.05), but nonsignificant between GL and PL at $16^{\circ}C$. .In NL digalactosyldiacylglycerol (3->$7^{\circ}$) increased but monogalactosyldiacylglycerol (10%) did not change at $25^{\circ}C$. In PL phosphatidic acid (22 -> 4%) and phosphatidylinositol (18 -> 5%) decreased but phosphatidyl ethanolamine (12->l6%) increased at $25^{\circ}C$. Percent unsaturated acid slightly decreased in NL and PL but greatly increased in GL at $25^{\circ}C$. Percent unsaturated bond slightly decreased in NL but did not change in PL and GL.