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Preparation of Black Panax ginseng Leaf and Evaluation of its Antioxidative Effect  

Kim, Sang-Kyum (College of Pharmacy, Chungnam National University)
Kim, Eui-Keom (College of Pharmacy, Chungnam National University)
Lee, Jee-Hyun (College of Pharmacy, Chungnam National University)
Cho, Soo-Hyun (College of Pharmacy, Chungnam National University)
Shen, Gui-Nan (College of Pharmacy, Chungnam National University)
Guo, Jin-Long (College of Pharmacy, Chungnam National University)
Oh, Jung-Min (College of Pharmacy, Chungnam National University)
Myung, Chang-Seon (College of Pharmacy, Chungnam National University)
Oh, Han-Jin (Cheil General & Women's Health Care Center, College of Medicine, Kwandong University)
Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
Yun, Mi-Young (Department of Pathology, College of Oriental Medicine, Daejeon University)
Yun, Jae-Don (Chungcheongnam-Do Provincial Government, Future Strategy Team)
Roh, Seong-Soo (Department of Herbalogy, College of Oriental Medicine, Daegu Haany University)
Park, Yong-Jin (Baeknam Oriental Medical Hospital)
Seo, Young-Bae (Department of Herbalogy, College of Oriental Medicine, Daejeon University)
Song, Gyu-Yong (College of Pharmacy, Chungnam National University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.2, 2008 , pp. 397-402 More about this Journal
Abstract
The purpose of this study is to prepare black Panax ginseng leaf (PGL) and evaluate its antioxidative effect. In order to make black PGL, the raw PGL was successiely steamed at $95^{\circ}C$ for 3 hr nine times. The antioxidant activities of total saponins (Sa) from PGL and black PGL against peroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. Specific TOSC values for black PGL-Sa against peroxyl radicals and peroxynitrites were 2.3-fold and 2.1-fold of PGL-Sa, respectively, and 2.2-fold and 5.2-fold of glutathione, a positive control antioxidant, respectively. The black PGL-Sa exhibited stronger antioxidative effect than PGL-Sa. The main ginsenosides of black PGL were $Rg_3,\;Rk_1\;and\;Rg_5$. Among the saponins in black PGL, the amount of ginsenoside $Rg_3$ was examined by HPLC. 22.12 mg of ginsenoside $Rg_3$ was obtained from 1g of dried black PGL.
Keywords
Panax ginseng leaf; Black Panax ginseng leaf; Antioxidative effect; TOSC assay; Ginsenoside$Rg_3$;
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Times Cited By KSCI : 3  (Citation Analysis)
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