• Journal of the Korea Convergence Society
• /
• v.8 no.8
• /
• pp.191-196
• /
• 2017

This study was conducted to investigate the effect of fine bubble water treatment on the growth of ginseng seedlings. The growth of ginseng seedlings which were treated with general water and fine bubble water was investigated. The above ground part, the growth of leaf was increased by about 10% and the growth of the stem was about 6%, as the ginseng seedlings which were treated with fine bubble water comparing to the ginseng seedlings treated with general water. Root length was increased about 5%, root width was increased about 8%, roots weight was increased about 9%, and dry weight was increased about 7%. This is not because the whole root growth was increased, but the main root growth was increased about 7%. These results suggested that the physical properties of the fine bubble water stimulated the growth of ginseng seedlings. These results are initial study in the case of ginseng seedlings. Therefore, it can be applicable to the 3-5 years old ginseng plants. Further research will be needed to find out the optimal cultivation condition by controlling the dissolved oxygen amount according to the soil condition and the research applied to the ginseng seedlings.

• Korean Journal of Medicinal Crop Science
• /
• v.22 no.5
• /
• pp.391-397
• /
• 2014

To control the disease of root rot in ginseng nursery, inorganic sulfur solution of 0.1%, 1.0%, and 2.0% were irrigated by amount of $10{\ell}$ per $3.3m^2$ before sowing. On the last ten days of July, Fusarium solani and F. oxysporum were similarly detected by 44.8% and 43.8%, respectively, while Cylindrocarpon destructans was low detected by 4.4% in the diseased seedling. The more sulfur's concentration was increased, the more soil pH was decreased. Soil pH was decreased from 5.87 to 4.59 by the irrigation of sulfur solution of 1.0%. The more sulfur's concentration was increased, the more electrical conductivity (EC) of soil was increased. EC was increased from 0.27 dS/m to 1.28 dS/m by the irrigation of sulfur solution of 1.0%. Irrigation of sulfur solution was effective on the inhibition of damping-off caused by Rhizoctonia solani in ginseng seedling. Control value for damping-off by the irrigation of sulfur solution of 1.0% and 2.0% were 75.7%, and 78.5%, respectively. Growth of leaf was inhibited by the irrigation of sulfur solution of 2.0%. Root weight per $3.3m^2$ showed the peak in sulfur solution of 1.0%, while survived-root ratio and root weight per plant were decreased in the level of 2.0%. Survived-root ratio of seedling in sulfur solution of 1.0% was distinctly increased by 4.7 times compare to the control, but control value for root rot was relatively low as 49.2%. Mycelium growth of C. destructans, F. solani, and R. solani were distinctly inhibited by the increase of sulfur's concentration in vitro culture using PDA medium.

• Journal of Ginseng Research
• /
• v.43 no.1
• /
• pp.38-48
• /
• 2019

Background: Interspecific ginseng hybrid, Panax ginseng ${\times}$ Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F1 progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid. Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with $GA_3$ to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS. Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L $GA_3$ for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The $F_1$ hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides $F_{11}$ in P. quinquefolius), and higher amount of other ginsenosides than its parents. Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants.

• Mycobiology
• /
• v.42 no.1
• /
• pp.12-16
• /
• 2014

We isolated and examined a new Alternaria sp., which causes leaf spots on Peucedanum japonicum in Korea, by using molecular and morphological methods. Phylogenetic analysis based on a combined internal transcribed spacer region analysis and two protein-coding genes (gpd and Alt a1) demonstrated that the causal fungus was most closely related to A. cinerariae and A. sonchi, and relevant to A. brassicae. However, conidial morphology indicated that it is a novel species within the genus Alternaria, and therefore we have assigned the fungus a new name in this study.

• Journal of the Korean Society of Tobacco Science
• /
• v.18 no.1
• /
• pp.5-11
• /
• 1996

Field experiment was conducted to get the basic information about the difference in color and plastid pigments at the different stage of harvest of flue-cured tobacco cultivated in paddy field, and compared to upland ones. All tested crops were primed in lots of 3 to 4 at each priming, beginning at the bottom of the plant. Leaf pigments and chromatic values of tobacco leaves were determined in samples collected at weekly intervals, and according to analyses obtained from 4 stalk positions. By the degree of maturity, leaves harvested were separated with visual characters into four classes of immature, mature, ripe and mellow. regardless of stalk position, chlorophyll contents was higher in the order of immature > mature > ripe > mellow, respectively. In fresh leaves, chlorophyll levels was higher with ascending stalk position. chlorophyll a and b in cured leaves were less than 10% of the amount present at harvest, and it was estimated that maturity condition also influenced the rates of degradation of plastid pigments. Fully mature leaves of upland diverted from paddy field showed slightly higher in chlorophyll contents than in those of upland tobacco ones but somewhat lowered in $L^*$ and $b^*$ values, and there was no difference in $a^*$ value.

• Journal of the Korean Society of Tobacco Science
• /
• v.16 no.1
• /
• pp.53-56
• /
• 1994

This study was aimed at finding out the effect of herbicide quinclorac (3, 7-dichloro-8- quinoline carbxylic acid) on tobacco culture in paddy soil. Herbicides quinclorac(Pozol, Pulta and Tomata) was randomly applied to subdivided paddy soil in Sep. 1992, and tobacco seedlings were transplanted to the field when seedling averaged 2cm in height in April 1993. Experiment plots were divided into non - treatment, standard(3kg/10a) and two times(6kg/10a) in amounts for each three herbicides. The symptoms of damage from herbicide were sighted when the plants had developed 8-9 leaves at 30 day after transplanting in standard amount plots, and when the plants had developed 4-5 leaves at 20 days after transplanting in two times treatments. The new leaves from damaged plants were bended out, and leaf color changed from green to dark - green, and then gradually advanced to abnormal narrow leaves. Standard treatments of herbicides showed a decrease of 18% in price per kg, 18% in yield and 33% in value per 10a than those of non - treatment, while two times treatments showed a decrease of 33%, 29%, 52% compared with non - treatment, respectively.

• Journal of Ginseng Research
• /
• v.27 no.1
• /
• pp.32-36
• /
• 2003

This experiment were conducted to select cheaper and more favorable compost among several kinds of composts which were produced by companies, farmers etc. instead of Yakto. The yield of usable ginseng seedling was also the highest in compost of Yakto, but that of usable seedling were inclined to be decreased in the compost of PL, Mushroom, Rice straw and HJ, in order. The content of inorganic salts in different kinds of composts was much different from individual organ part including leaf, stem and root. It was concluded that fully decomposed manure should be used and it needs to investigate perfect decomposed composts of PL and Mushroom instead of Yakto in order to yield ginseng seedings

• Korean Journal of Soil Science and Fertilizer
• /
• v.18 no.1
• /
• pp.32-37
• /
• 1985

Effects of straw mulching on growth of shoot and root of Panax ginseng, soil physical and chemical characteristics were investigated. Straw mulching increased root yield, stem length, leaf area and decreased defoliated plant rate and missing root rate. Percent solid phase and soil hardness decreased, and porosity, percent air phase and percent liquid phase increased under straw mulch. It increased soil water content and decreased soil temperature especially in the rear side of the bed. Available soil phosphorus (very harmfully in some case), exchangeable potassium, organic matter increased while exchangeable calcium decreased and the lowering of pH was retarded under mulch. Straw mulching greatly reduced need population, prevented soil covering on the lower side of leaves and eliminated solings to plants, bed sides and on bed top.

• Korean Journal of Medicinal Crop Science
• /
• v.20 no.4
• /
• pp.217-221
• /
• 2012

This research was conducted to investigate the influence of various organic substrates on growth and yield of ginseng seedling grown organically in the closed plastic house. The pH and EC of substrates used for organically ginseng cultivation ranged 5.93~6.78 and 0.03~0.15 dS/m respectively. The concentrations $NH_4$-N and $NO_3$-N respectively was 14.01~68.63 mg/L, 5.60~58.83 mg/L. The average quantum of the closed plastic house was range from 10 to 16% of natural light. In July and August, the maximum temperature of the closed plastic house did not exceed 30 and the average temperature was maintained within 25 lower than the field because air conditioning ran. The PPV-1 and PPV-2 bed soil substrates produced higher stem length, stem diameter, shoot fresh weight and leaf area than those of conventional culture. In PPV-2 bed soil substrates, root fresh weight and root diameter was the highest. The root fresh weight of PPV-2 bed soil substrates in closed plastic house was maximum 25% heavier than the conventional cultivation. The results of this experiment will be utilized for making new substrate application for organic ginseng culture in the plastic house.

• Journal of Korean Society of Forest Science
• /
• v.99 no.6
• /
• pp.863-870
• /
• 2010

We investigated geographical condition and soil characteristic of ginseng cultivation site. At all sites, crown density adjusted by 80%. and Air and soil temperature were also measured. The geographical condition vary ato all sites. and soil shows similar characteristics with typical forest soil of Korea. The results shows the Air temperature needs to be higher than $15^{\circ}C$ for seed budding at April When soil temperature reach at 8, leaf of foest ginseng starts to bud. A forest ginseng is influenced by forest type, planting type and budding rates. In the case of a seedling planting, an seeding emergence rate is high, but the rate is decreased rapidly after three years On the other hand, direct seeding shows lower seedling emergence rate, but survival rate is higher than seedling-planting.