• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.149-154
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• 2000

Embryogenic suspension cultures were initiated using embryogenic callus from immature inflorescence explants (Aralia cordata Thunb.) cultured on solid MS medium containing 1 mg/L 2,4-D for 8 weeks and then the embryogenic callus was proliferated in liquid MS medium containing 1 mg/L 2,4-D. After sieving the suspensions (pore size 270$\mu$m), embryogenic cells were cultured in liquid MS medium with cytokinins (kinetin, BA, zeatin) for two weeks. When the embryogenic cells were transferred to liquid MS basal medium, primary somatic embryos were developed after 5 weeks of culture. Secondary embryos were developed directly from the primary torpedo and cotyledonary embryos cultured in solid MS basal medium. Frequency of secondary embryogenesis was higher on medium containing 2 mg/L kinetin than the other cytokinins. Plant regeneration was highly recorded by placing secondary cotyledonary embryos induced from primary cotyledonary embryos in MS medium containing 2 mg/L kinetin or 2 mg/L zeatin (25.4% and 28.6%, respectively). The plant regeneration from secordary embryos was prohibited by tertiary embryogenesis.

• Korean Journal of Weed Science
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• v.4 no.2
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• pp.130-134
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• 1984

This experiment was conducted to know critical sowing depth and emergence day under different sowing depth at Crop Experiment Station, Suweon, in 1982. Tested weed species were Sagittaria pygnaea, Eleocharis kuroguwai, Cyperus serotinus and Potamageton distinetus, sowing depth of weeds were 5cm, 10cm, 15cm, 20cm and 25cm Critical emergence sowing depth of S. pygnaea, E. kuroguwai was 15cmand C. serotinus was 20cm sowing depth while P. distintus was 25cm sowing depth. Average emergence days under 5cm sowing depth for E. kurogwai, S. pygmaea, P. distintus and C. serotinus was 27.8 days, 14.1 days, 13.6 days and 7.3 days. While, relationships between sowing depth and day to emergence was positively correlated. Gernination percent of weeds under Critical emergence sowing depth, S. pygmaea, E. kurogwai and, P. distinctus was 57%, 67%, 80%, but C. serotinu.s was all died.