• Horticulture, Environment, and Biotechnology : HEB
• /
• 제59권6호
• /
• pp.875-888
• /
• 2018

Tulip (Tulipa L.) is one of the most important ornamental geophytes in the world. Analysis of molecular variability of tulips is of great importance in conservation and parental lines selection in breeding programs. Of the 70 genic microsatellites, 15 highly polymorphic and reproducible markers were used to assess the genetic diversity, structure, and relationships among 280 individuals of 36 wild and cultivated tulip accessions from two countries: Iran and the Netherlands. The mean values of gene diversity and polymorphism information content were 0.69 and 0.66, respectively, which indicated the high discriminatory power of markers. The calculated genetic diversity parameters were found to be the highest in wild T. systola Stapf (Derak region). Bayesian model-based STRU CTU RE analysis detected five gene pools for 36 germplasms which corresponded with morphological observations and traditional classifications. Based on analysis of molecular variance, to conserve wild genetic resources in some geographical locations, sampling should be performed from distant locations to achieve high diversity. The unweighted pair group method with arithmetic mean dendrogram and principal component analysis plot indicated that among wild tulips, T. systola and T. micheliana Hoog exhibited the closest relationships with cultivated tulips. Thus, it can be assumed that wild tulips from Iran and perhaps other Middle East countries played a role in the origin of T. gesneriana, which is likely a tulip species hybrid of unclear origin. In conclusion, due to the high genetic variability of wild tulips, they can be used in tulip breeding programs as a source of useful alleles related to resistance against stresses.

• 한국환경복원기술학회지
• /
• 제25권3호
• /
• pp.29-45
• /
• 2022

For biodiversity conservation, the importance of beta-diversity which is changes in the composition of species according to environmental changes has become emphasized. However, given the systematic investigation of species distribution and the accumulation of large amounts of data in the Republic of Korea(ROK), research on the spatialization of beta-diversity using them is insufficient. Accordingly, this research investigated the applicability of the Generalized Dissimilarity Modeling(GDM) to ROK, which can predict and map the similarity of compositional turnover (beta-diversity) based on environmental variables. A brief overview of the statistical description on using GDM was presented, and a model was fitted using the flora distribution data(410,621points) from the National Ecosystem Survey and various environmental spatial data including climate, soil, topography, and land cover. Procedures and appropriated spatial units required to improve the explanatory power of the model were presented. As a result, it was found that geographical distance, temperature annual range, summer temperature, winter precipitation, and soil factors affect the dissimilarity of the vegetation community composition. In addition, as a result of predicting the similarity of vegetation composition across the nation, and classifying them into 20 and 100 zones, the similarity was high mainly in the central inland area, and tends to decrease toward the mountainous areas, southern coastal regions, and island including Jeju island, which means the composition of the vegetation community is unique and beta diversity is high. In addition, it was identified that the number of common species between zones decreased as the geographic distance between zones increased. It classified the spatial distribution of plant community composition in a quantitative and objective way, but additional research and verification are needed for practical application. It is expected that research on community-level biodiversity modeling in the ROK will be conducted more actively based on this study.

• Animal Systematics, Evolution and Diversity
• /
• 제7권1호
• /
• pp.127-150
• /
• 1991

한국산 산호충류 중 돌산호류의 계통분류학적 연구를 하기 위하여 1969년부터 1986년까지 우리나라의 삼면연안과 도시지방(35개지역)으로부터 채집된 표본들을 동정 분류하고 지리적 분포도 고찰하였다. 그 결과 5아목 7과 15속 24종이 밝혀졌으며 이 중에서 7종은 한국 미기록종이었다. 한국산 돌산호류를 분포형으로 보면 열대형(16종, 66.7%)과 온대형(8종, 33.3%)으로만 되어있고, 해역별로 보면 제주도 해역에 16종(4온대종,12열대종)으로 가장 많은 종이 분포하였으며 황해에 3종 (2온대종, 1열대종)으로 가장 적은 종이 분포하였다. 해역간 유사계수를 근거로 보면 대한해협과 동해 사이의 유사성(0.596)이 가장 크고, 황해와 제주도해역 사이의 유사성(0)은 전혀없었다.

• 동의생리병리학회지
• /
• 제34권6호
• /
• pp.309-318
• /
• 2020

Besides respiratory infection, COVID-19 has many neurological symptoms not only loss of smell and taste but also fatigue and brain fog. But it is a challenge to treat the neurological symptoms especially of anosmia and ageusia. In order to search for the therapeutic methods, the geographical diversity and pathological mechanisms of the COVID-19 and two symptoms were investigated from the latest clinical studies. Because the environmental conditions of the monsoon climate zone of East Asia and the Mediterranean and Oceanic climate zone of Italy, Britain, United States and tropical Brazil are different, each of diverse etiology and internal milieu should be considered differently in the treatment. SARS-CoV-2 exhibits the dampness-like characteristics and the olfactory and gustatory disorders are particularly more common than other flu or cold. and it tends to show features of damaging the lung qi of olfaction and heart-spleen qi of gustation. The mechanisms of olfactory and gustatory loss are various according to precursory, inflammatory, non-inflammatory and sequelar forms, so the therapeutic method should be designed for each period and pathology. If the process of inflammation arises from nasal and respiratory, olfactory epithelium to the central nervous structure by way of blood brain barrier, the treatment should be corresponded with the stage and depth of pathogen place. And if the olfactory loss is asymptomatic or in the initial stage, it can be applied intranasal topical scent therapy to relieve temporary locking of qi movement, but maybe also used in parallel together with herbs of relieving dampness toxin latent in the lung parenchyma.

• 한국경제지리학회지
• /
• 제26권2호
• /
• pp.73-95
• /
• 2023

본 연구의 목적은 산업도시 쇠퇴의 대표적 업종 중 하나인 조선업 특화 도시들의 산업구조 변화 및 유형을 분석하고 지역별 대응 방안을 모색하는 것이다. 2018년 산업위기대응특별지역으로 지정된 도시의 대부분이 조선업에 특화되어 있는데, 이것은 2010년대 이후 호황에서 불황으로 전환되는 경기변동이 주된 요인이지만 도시마다 산업위기에 대응하는 방식은 상이하였다. 조선업 특화 도시 7곳을 대상으로 분석한 결과, 조선업의 경기변동은 산업경쟁력이 높은 대기업 조선소가 입지한 지역들이 상대적으로 덜 쇠퇴하였고, 호황기에는 산업구조의 다양성이 감소하고 불황기에는 증가하는 경향이 나타났다. 산업위기에 대한 저항력과 회복력으로 조선업 특화 도시의 산업 경로를 4가지 유형으로 구분하였다. 지역별 산업구조 및 경쟁력을 고려하여 조선업의 유지와 쇠퇴, 새로운 대안 산업의 육성 등 맞춤형 지원전략이 필요한 시점이다.

• 한국작물학회지
• /
• 제46권2호
• /
• pp.112-120
• /
• 2001

Genetic diversity of 47 East-Asian vegetable soybean was characterized by means of agro-morphological traits and RAPD markers. A field trial was conducted to evaluate 14 agro-morphological traits. To study RAPD-based DNA analysis, a total of sixty 10-mer random primers were screened. Of these, 23 polymorphic markers in 16 varieties used for screening. Among 207 markers amplified, 48 were polymorphic for at least one pairwise comparison within the 47 varieties. A higher differentiation level between varieties was observed by using RAPD markers compared to morphological markers. Correspondence analysis using both types of marker showed that RAPD data could fully discriminate between all varieties, whereas morphological markers could not achieve a complete discrimination. Genetic distances between the varieties were estimated from simple matching coefficients, ranged from 0.0 to 0.640 with an average of 0.295$\pm$0.131 for morphological traits and 0.042 to 0.625 with an average of 0.336$\pm$0.099 for RAPD data, respectively. Cluster analysis based on genetic dissimilarity of these varieties gave rise to 4 distinct groups. The clustering results based on RAPDs did not match with those based on morphological traits. Geographical distribution of most varieties in each of the groups were not well defined. The results suggested that the level of genetic diversity within this group of East-Asian vegetable soybean varieties was sufficient for a breeding program and can be used to establish genetic relationships among them with unknown or unrelated pedigrees.

• 한국작물학회지
• /
• 제65권1호
• /
• pp.1-21
• /
• 2020

Association mapping is widely used in rice and other crops to identify genes underlying important agronomic traits. Most association mapping studies use diversity panels comprising accessions with various geographical origins to exploit their wide genetic variation. While locally adapted breeding lines are rarely used in association mapping owing to limited genetic diversity, genes/alleles identified from elite germplasm are practically valuable as they can be directly utilized in breeding programs. In this study, we analyzed genetic diversity of 179 rice varieties (161 japonica and 18 Tongil-type) released in Korea from 1970 to 2006 using 192 microsatellite markers evenly distributed across the genome. The 161 japonica rice varieties were genetically very close to each other with limited diversity as they were developed mainly through elite-by-elite crosses to meet the specific local demands for high quality japonica rice in Korea. Despite the narrow genetic background, abundant phenotypic variation was observed in heading time, culm length, and amylose and protein content in the 161 japonica rice varieties. Using these varieties in association mapping, we identified six, seven, ten, and four loci significantly associated with heading time, culm length, and amylose and protein content, respectively. The sums of allelic effects of these loci showed highly significant positive correlation with the observed phenotypic values for each trait, indicating that the allelic variation at these loci can be useful when designing cross combinations and predicting progeny performance in local breeding programs.

• Journal of Veterinary Science
• /
• 제22권3호
• /
• pp.37.1-37.14
• /
• 2021

Background: Ticks are one of the most common external parasites in dogs, and are associated with the transmission of a number of major zoonoses, which result in serious harm to human health and even death. Also, the increasing number of pet dogs and pet owners in China has caused concern regarding human tick-borne illnesses. Accordingly, studies are needed to gain a complete understanding of the bacterial composition and diversity of the ticks that parasitize dogs. Objectives: To date, there have been relatively few reports on the analysis of the bacterial community structure and diversity in ticks that parasitize dogs. The objective of this study was to investigate the microbial composition and diversity of parasitic ticks of dogs, and assessed the effect of tick sex and geographical region on the bacterial composition in two tick genera collected from dogs in China. Methods: A total of 178 whole ticks were subjected to a 16S ribosomal RNA (rRNA) next generation sequencing analysis. The Illumina MiSeq platform targeting the V3-V4 region of the 16S rRNA gene was used to characterize the bacterial communities of the collected ticks. Sequence analysis and taxonomic assignment were performed using QIIME 2 and the GreenGene database, respectively. After clustering the sequences into taxonomic units, the sequences were quality-filtered and rarefied. Results: After pooling 24 tick samples, we identified a total of 2,081 operational taxonomic units, which were assigned to 23 phyla and 328 genera, revealing a diverse bacterial community profile. The high, moderate and low prevalent taxa include 46, 101, and 182 genera, respectively. Among them, dominant taxa include environmental bacterial genera, such as Psychrobacter and Burkholderia. Additionally, some known tick-associated endosymbionts were also detected, including Coxiella, Rickettsia, and Ricketssiella. Also, the potentially pathogenic genera Staphylococcus and Pseudomonas were detected in the tick pools. Moreover, our preliminary study found that the differences in microbial communities are more dependent on the sampling location than tick sex in the tick specimens collected from dogs. Conclusions: The findings of this study support the need for future research on the microbial population present in ticks collected from dogs in China.

• Journal of Ecology and Environment
• /
• 제45권3호
• /
• pp.97-104
• /
• 2021

Background: Asarum sieboldii Miq., a species of forest understory vegetation, is an herbaceous perennial belonging to the family Aristolochiaceae. The metapopulation of A. sieboldii is distributed sparsely and has a short seed dispersal distance by ants as their seed distributor. It is known that many flowers of A. sieboldii depend on self-fertilization. Because these characteristics can affect negatively in genetic structure, investigating habitat structure and assessment of genetic structure is needed. A total of 27 individuals in a valley were sampled for measuring genetic diversity, genetic distance, and genetic differentiation by RAPDPCR. Results: The habitat areas of A. sieboldii metapopulation were relatively small (3.78~33.60 m²) and population density was very low (five to seven individuals in 20×20 m quadrat). The habitat of A. sieboldii was a very shady (relative light intensity = 0.9%) and mature forest with a high evenness value (J = 0.81~0.99) and a low dominance value (D = 0.19~0.28). The total genetic diversity of A. sieboldii was quite high (h = 0.338, I = 0.506). A total of 33 band loci were observed in five selected primers, and 31 band loci (94%) were polymorphic. However, genetic differentiation along the valley was highly progressed (G_st = 0.548, N_m = 0.412). The average genetic distance between subpopulations was 0.387. The results of AMOVA showed 52.77% of variance occurs among populations, which is evidence of population structuring. Conclusions: It is expected that a small-scale founder effect had occurred, an individual spread far from the original subpopulation formed a new subpopulation. However, geographical distance between individuals would have been far and genetic flow occurred only within each subpopulation because of the low density of population. This made significant genetic distance between the original and new population by distance. Although genetic diversity of A. sieboldii metapopulation is not as low as concerned, the subpopulation of A. sieboldii can disappear by stochastic events due to small subpopulation size and low density of population. To prevent genetic isolation and to enhance the stable population size, conservative efforts such as increasing the size of each subpopulation or the connection between subpopulations are needed.

• 한국버섯학회지
• /
• 제18권4호
• /
• pp.323-330
• /
• 2020

본 연구에서는 유럽 양송이 자원들을 SSR marker를 통해 유전적 다양성과 집단 구조, 유전적 분화에 대하여 분석하였다. 본 연구에서 유럽의 양송이 자원들은 유전적 거리기반의 4개의 그룹으로 나뉘었고 집단구조 분석을 통하여 2개의 subpopulation으로 이루어져 있었다. 본 연구에서 사용한 SSR 마커로 유럽의 양송이 자원들은 지리적 그리고 갓색으로 구분되지 않았다. 유전적 다양성은 유전적 거리기반의 그룹에서는 Group 4, 집단구조 분석을 통한 subpopulation에서는 Pop. 2의 다양성이 높았다. 그리고 양송이 자원들은 유전적 분화가 매우 낮았다. 본 연구의 결과는 차후 양송이의 육종 등에 이용 할 수 있을 것이다.