• Journal of Aquaculture
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• v.11 no.1
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• pp.105-111
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• 1998

For ecological studies on the habitat of geoduck clam, Panope japonica caught at the coastal water of Kangwon province located in the East of Korea. The annual water temperature of the geoduck's fishing grounds was ranged from 5.6 to $17.5^{\circ}C.$. The salinity was not so fluctuated showing from 32.27 to 34.22${\textperthousand}$, and dissolved oxygen was ranged 5.51 to 7.27 ml/l. Sediments where geoduck was distributed chiefly was consisted of very fine sands of 3.0 to 4.0 phi (49.2%) and find sand of 2.0 to 3.0 phi scale (31.87%). The shell length of geoduck was measured 70 to 135 mm (mean : 107.70mm) and the weight was 50 to 550g (mean : 299.63g). The total weight (TW) against the shell length (SL) could expressed as the regression equation as TW=$1.4446{\times}10^{-3}SL^{2.6067}$.

• Journal of Aquaculture
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• v.10 no.2
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• pp.213-218
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• 1997

Experiments on the feeding activity and environmental tolerance of geoduck clam, Panope japonica caught at the coastal water of Kangwon province located in the East Sea of Korea were performed from January to December, 1994. Twenty genera and 33 species of plankton were found in the digestive tract, and most of them are phytoplankton. In spring, 21 species of planktonic foods were indentified, but in sumer, only 11 species were identified. Nitzschia longissima and Rizosolenia alata were present evey month. Weight of the digestive tract was fluctuate seasonally : higher winter and spring, and lower summer. Adult geoduck clams could be tolerated within the range of 5 to $20^{\circ}C$ water temperature, and they could tolerated considerably well temperature than higher temperature within the range.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.6
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• pp.549-556
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• 1993

To obtain a basic data of Korean geoduck(Panope japonica A. Adams) for fast food manufacturing, contents of proteinous compounds, nucleic acid and related compounds were analysed, and monthly changes of proximate compositions and freshness changes during frozen storage have been studied. The edible portions were $29.92\%$ in 3 years old samples and $38.04\%$ in 5 years old ones, respectively. The moisture and protein content showed the highest level from March to April and those of lipid and glycogen showed the highest level in July. Taurine, glycine, alanine and glutamic acid were the major free amino acid in 5 years old samples harvested in July and taurine content showed the highest level among free amino acids. Glutamic acid was the most abundant amino acid among the amino acids of protein hydrolyzate in 5 years old sample(96.6mg/16g-N). Leucine and lysine were revealed as relatively higher content(8.35 and $7.70\%$). From the analytical results on VBN, TMAO, TMA and $NH_2-N$, blanching at $95^{\circ}C$ for 2 mins was effective to maintain the quality of geoduck during frozen storage. Total creatine content was not changed in blanched geoduck.

• Journal of fish pathology
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• v.33 no.2
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• pp.127-138
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• 2020

We attempted to isolate and identify potentially pathogenic bacteria from geoduck clam (Panopea japonica) larvae, juvenile and adult, focusing on Vibrios. The isolates were identified by molecular approach and biochemical characterization. In particular, we applied MLSA (multilocus sequence analysis) to the isolated Vibrios for clear identification and phylogenetic relationships, by combining 16s rDNA and several houskeeping genes (pyrH, recA, rpoA). We obtained 141 isolates; 10 from healthy adults, 52 from moribund adults with blisters and 79 from larvae. 46 from the moribund adults and 39 from the larvae were identified as Vibrio species, while the rest of these samples and all the isolates from healthy adult were identified as marine general bacteria. Among Vibrio species, Vibrio splendidus was the most frequently identified from the moribund adults and clustered with the known V. splendidus in GenBank by MLSA. However, it was still unclear that V. splendidus was the cause of blisters because the artificial infection experiment was not conducted and V. splendidus was isolated also from the larvae. Further studies are necessary to clarify the etiological agent of the blisters found in geoduck clam in this study.

• Journal of Fisheries and Marine Sciences Education
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• v.3 no.2
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• pp.47-72
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• 1991

To obtain the principal data for useful treatment and processing of Korean geoduck (Panope japonica A. ADAMS) which inhabit mostly at Dong-Hae coastal area in Korea, changes of $NH_2$-N, TMAO, TMA, total creatinine, protein composition and fatty acid composition in raw and blanched geoduck muscle during storage at $-20^{\circ}C$ were investigated. In addition, its chemical composition variation in the whole year was elucidated. The moisture content in geoduck muscle meat was 78.1% to 82% in the whole year. Particularly, in July its moisture content was maximum as 82% and in September minimum as 78.1%. Crude protein was in the range of 12.3-16.4%, crude lipid the average was 1.5%, crude ash on the average was 1.4%. The abundant fatty acids in geoduck muscle oil were $C_{16}$ : 0, $C_{16}$ : 1, $C_{18}$ : 0, $C_{18}$ : 1, $C_{20}$ : 5, and $C_{22}$ : 6 acids. During storage at $-20^{\circ}C$, content of unsaturated fatty acid such as eicosapentaenoic acid (EPA, $C_{20}$ : 5) and docosahexaenoic acid (DHA, $C_{22}$ : 6)in raw geoduck muscle decreased somewhat and the raw geoduck was slightly oxidized. Trimethylamine (TMA), volatile basic nitrogen (VBN)and $NH_2$-N of raw muscle increased compared to blanched muscle. Trimethylamine oxide (TMAO) was slightly decreased during the storage period. The muscle protein was approximately composed of 37% sarcoplasmic, 29% myofibrillar, 22% alkali soluble, and 12% stroma protein. Among several proteins, myofibrillar protein content decreased mostly, while the alkali-soluble and stroma protein content increased slightly during storage at $-20^{\circ}C$.

• The Korean Journal of Malacology
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• v.30 no.4
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• pp.303-309
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• 2014

The development of Japanese geoduck, Panopea japonica, grown under culture conditions, has been examined through the morphological characteristics in fertilized egg, larvae and juvenile. Gametes were stripped from ripe broodstock and placed into two separate containers. Eggs were washed through a $40{\mu}m$ sieve and fertilized with dilute sperm solution. Developing larvae were maintained at $19{\pm}1^{\circ}C$. Fertilized eggs with $81.6{\mu}m$ diameter developed to trochophores within 14 h and to D-stage larvae ($116{\mu}m$ shell length) within 27 h. Larvae were spontaneously settled at shell length of $311{\mu}m$ after 20 days. The hatching from fertilized eggs and larval rearing were normally available in $18.5-21.5^{\circ}C$, and the growth was good in a cashmilon substrate, as well as sand. After rearing of day 108 from metamorphosis, the shell length of juvenile P. japonica reached 13 mm, and growth rate of shell length of the juvenile was $117.5{\mu}m/d$.

• The Korean Journal of Malacology
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• v.31 no.4
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• pp.263-266
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• 2015

This study was performed to determine the effect of water temperature condition on survival rate and growth of juvenile geoduck (Panopea japonica). Panopea japonica juveniles were bred for 6 weeks at 12, 15, 18, 21, 24 and $27^{\circ}C$ on incubator. Juveniles median lethal times (LT50) were 29 hours in $27^{\circ}C$, 14.5 day in $24^{\circ}C$, 37.4 day in $21^{\circ}C$. Survival rate of water temperature 12, 15 and $18^{\circ}C$ showed a high survival rate in 95.0%, 95.0% and 93.3% at 6 weeks. As a result of culturing for 6 weeks at 12, 15, 18, 21 and $24^{\circ}C$, a daily growth of shell length and total weight was 0.10 mm, 0.12 mm, 0.13 mm, 0.16 mm and 0.14 mm, and 2.21 mg, 2.65 mg, 2.84 mg, 3.13 mg and 2.93 mg. Juvenile shell length and total weight have significantly increased at $15-21^{\circ}C$. However, mortality rate has significantly increased at more than $21^{\circ}C$. As a result, appropriate water temperature for juveniles culturing considered $15-18^{\circ}C$.

• Journal of Aquaculture
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• v.10 no.2
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• pp.113-121
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• 1997

In order to developed the techniques for artificial seedling production of geoduck clam, Panope japonica, various stimulution for spawning induction and hatching condition were studied. Spawning induction by the air day or UV-irradiation stimulus were not effective. Water temperature stimulus was responsed 15.0~25.0% in May and 10.0% in June. But spawning induction by the gonad incision was highest with 15.0~45.0%. Ammonium hydroxide (NH4OH) stimulus adding in seawater were responsed 15.0% at 8/1000N~10/1000N, and ammonium hyhroxide solution injected in the gonads were responsed 5.0~10.0% at 5/100N~7/100N. The highest fertilization and hatching rate at various water temperature were ranged 74.5~89.2% in 11~$17^{\circ}C, \;84.3~89.5%\;in\;8~14^{\circ}C$, respectively. the highest fertilization rate and hatching rate in various salinity were ranged 72.5~88.5% in 25~$35tetperthousand$, 82.7~86.9% in 30~$35tetperthousand$, respectively. The optimum water temperature and salinity for fertilization and hatching to the 11~14$^{\circ}C$ and 30~$35tetperthousand$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.4
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• pp.296-304
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• 1993

The effects of pretreatment on the lipid oxidation of korean geoduck muscle during storage at $-20^{\circ}C$ were investigated. In the lipid compositions of geoduck muscle, neutral, glycolipid, and phospholipid contents were $46.1\%,\;4.5\%\;and\;49.5\%$, respectively. And the content of phospholipid decreased to $39.9\%$ by blanching. In the fatty acids composition, $C_{16:0},\;C_{20:5}\;and\;C_{22:6}$ fatty acids were major portions and their contents showed, respectively, $10\%$ of total fatty acids amount or more. $C_{18:0},\;C_{16:1},\;C_{18:1},\;C_{14:0}\;and\;C_{20:1}$ fatty acids were also important fatty acids and their amounts were so much as $5\%$ or more. The polyunsaturated fatty acid contents were relatively high as $39.25\%{\sim}42.60\%$. POV in RM and VM samples increased gradually and showed 3.72meq/kg, or 3.00meq/kg after 90 days storage, respectively. However, in case of samples pretreated by blanching, there was slightly increase of POV throughout storage. From the results above, it was suggested that the pretreatment of blanching was effective in retardation of lipid oxidation for the longterm storage.

• Journal of Aquaculture
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• v.10 no.1
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• pp.25-32
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• 1997

Develoment precess and characteristics of eggs of the geoduck clam, Panope japonica are reporting in this study. Eggs and sperm were excised from gonad, artificially fertilized in an aquarium, reared under various temperature regimes, and record and record the larval period and the time need to reach a certain larval stage from ferilization. Unfertilized eggs of P. japonica appeared to be oval with a mean diameter of $70\mu$m and they became spherical after fertilization. The eggs of P. japonica can be classified as demersal. At a constant water temperature of $ 11^{citc}C$, it took 4 hours form fertilization to become four-cell stage, two days to become trochophore larvae, three days to become D-shape larvae, twenty-three days to become umbo stage, and thirty-six days to become fully grown veliger ready form settlement. A negative correlation was observed between the water temperature and the larval period of P. japonica. From fertilization to D-shape larvae, it took five days at 8$^{\circ}C$, while it was only two days to become D-shape larvae at $ 17^{citc}C$. Time required to D-shape larvae from fertilization was proportional to temperature, and the relationships were expressed as follows : To 8-cell stage, 1/t=0.0209 w-0.1167 (r=0.9967) To blastula stage, 1/t=0, 0055 w-0.0192 (r=0.9825) To trochophore stage, 1/t=0.0034 w-0.0155 (r=0.9907) To D-shape larvae stage, 1/t=0.0014 w-0.0023 (r=0.9843) (t, time in hours ; w, water temperature) Bioligical minimum temperature for egg development was calculated as 3.82$^{\circ}C$ in average.