• Journal of Plant Biotechnology
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• 제37권4호
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• pp.456-464
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• 2010

Potato is one of the most important crops in the world. Due to vegetative propagation of this crop, techniques of plant tissue culture and genetic transformation are often applied for potato researches and a lot of progress has been made in the breeding programs using these techniques during the last decades. In potato, there have been several trials to introduce GM potato varieties to the world market, but they so far failed due to the changed legislation and unwillingness of large processors to process GM potatoes. These issues are highly associated with the general acceptances of the public and other political decisions. In addition to these, there are still obstacles to overcome to achieve the development of commercial potato variety and several factors to improve horticulturally important traits. In this study, therefore, we reviewed recent advances and research status on tissue culture and genetic transformation in potato and discussed future perspective.

• Journal of Plant Biotechnology
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• 제48권2호
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• pp.93-99
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• 2021

This study was conducted to develop an Agrobacterium-mediated genetic transformation protocol for the carnation cv. "Jinju" to counteract its ethylene sensitivity. The new protocol involves the use of an improved shoot regeneration medium, optimized minimal concentrations of the selective agent, a pre-culture period, and co-cultivation periods. Silver nanoparticles (NAg) added at a concentration of 2.0 μM to the Murashige and Skoog (MS) basal shoot regeneration medium supplemented with 0.1 mg/L indole-3-butyric-acid (IBA) and 0.2 mg/L thidiazuron (TDZ) improved the shoot regeneration efficiency, number of shoots per explant, and plant growth compared to the control without the addition of NAg. The phosphinothricin (PPT) concentration of 1.0 mg/L was determined to be the minimal and optimal concentration for the selection of putative transgenic plants. When the explants were infected with Agrobacterium cells harboring the acdS gene, the explants that were pre-cultured for three days induced more putative transgenic plants than those that were co-cultivated for four days. Therefore, we expect that the results of this study will benefit researchers who are developing genetic transformations of carnations.

• Journal of Plant Biotechnology
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• 제6권1호
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• pp.1-7
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• 2004

Forestry sector has witnessed some unprecedented events in the recent past both in terms of galloping biotechnological developments and heated environmental debates over risks associated with release of transgenic trees. Improvements in the in vitro propagation techniques has made it possible to develop tissue culture based plant regeneration protocols just for about any tree species. And with the inclusion of every new species within the realms of tissue culture technology, it becomes a candidate for genetic improvement through recombinant DNA technology, the so called genetic engineering. Poplars and their hybrids serve as the model tree species on which most of the genetic transformation work as been carried out. A lot of work has also gone in genetic transformation of fruit trees and trees of horticultural interests. Trees have been successfully transformed for traits ranging from reduction of length of juvenile phase to alteration of tree architecture to altering wood quality by lignin and cellulose modification. More-over trees have been genetically engineered successfully to combat various types of insect pests and pathogens causing diseases. But all these developments have ignited controversies over the possible benefits and risks associated with transgenic plantations by various environmental agencies and activists. Solutions to most of these concerns can be found out with more intensive prioritized research.

• 한국미생물·생명공학회지
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• 제18권5호
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• pp.454-459
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• 1990

식물근부균 Fusarium solani의 생육을 강력히 길항하는 생물방제균 Pseudomonas stutzeri YPL-1에 외부유전자 도입을 통한 유전공학적 육종방법의 기초를 확립하고자 하였다. 이를 위해 plasmid pKT230을 vector로 하여 형질전환의 가능성을 조사하였으며 이때, 혈질전환에 필요한 최적조건을 조사한 결과 P.stutzeri YPL-1의 형질전환에는 대수증식기 초기의 균체가 가장 적합하였고, 20mM RbCl과 100mM $CaCl_2$를 함유한 냉각용액에 1${\mu}g$/ml의 plasmid DNA를 첨가하였을 때 최대의 형질전환 빈도를 나타내었다. 또한 plasmid DNA와 competent cell를 혼합한 후 $0^{\circ}C$에서 60분간 처리하는 것이 가장 효과적이었으며 이와 같은 조건에서 형질전환 빈도는 2~$6 \times 10^{-6}$으로 나타났다.

• Journal of Ginseng Research
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• 제36권3호
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• pp.291-297
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• 2012

Ginsenoside (ginseng saponin), the principal component of ginseng, is responsible for the pharmacological and biological activities of ginseng. We isolated lactic acid bacteria from Kimchi using esculin agar, to produce ${\beta}$-glucosidase. We focused on the bio-transformation of ginsenoside. Phylogenetic analysis was performed by comparing the 16S rRNA sequences. We identified the strain as Lactobacillus (strain 6105). In order to determine the optimal conditions for enzyme activity, the crude enzyme was incubated with 1 mM ginsenoside Rb1 to catalyse the reaction. A carbon substrate, such as cellobiose, lactose, and sucrose, resulted in the highest yields of ${\beta}$-glucosidase activity. Biotransformations of ginsenoside Rb1 were analyzed using TLC and HPLC. Our results confirmed that the microbial enzyme of strain 6105 significantly transformed ginsenoside as follows: Rb1${\rightarrow}$gypenoside XVII, Rd${\rightarrow}$F2 into compound K. Our results indicate that this is the best possible way to obtain specific ginsenosides using microbial enzymes from 6105 culture.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.327-335
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• 2009

Creeping bentgrass (Agrostis stolonifera L.) is economically important as the principal turfgrass species for golf course greens and fairways in temperate climates around the world. As the utilization area of the turfgrass species increases recently, the demand for new and improved cultivars increases. Thus, substantial progress has been made in applying modern biotechnology to develop genetically engineered (i.e., biotech) creeping bentgrass with new traits that eluded the breeders. This review article addresses the advances made in developing biotech creeping bentgrass, which are categorized in the following topics: (i) genetic transformation of creeping bentgrass, (ii) development of various biotech creeping bentgrasses by genetic engineering, and (iii) progresses in the deregulation of herbicideresistant creeping bentgrass.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.298.2-298
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• 1997

No Abstract, See Full Text

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2008년도 춘계학술발표회
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• pp.40-40
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• 2008

• 정보처리학회논문지B
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• 제17B권2호
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• pp.169-176
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• 2010

본 논문은 사용자 취향 학습 방법으로 대화형 유전자 알고리즘을 이용한 감정 기반 영상의 색 변환을 제안한다. 색 변환은 주로 조화로운색상의 범위를 정의한 기존의 템플릿을 기반으로 영상의 채도와 명도를 제외한 색상만을 변환하는 방법으로 수행되어 왔다. 색상의 조화를 조절함으로써 부자연스러운 영상을 자연스럽게 변환하여 영상을 감상하는 사용자의 만족도를 높였다. 한편, 색은 사람의 감정과도 밀접한 관련이 있기 때문에, 색과 감정의 관계를 정의 하면, 감정 기반 콘텐츠와 기술 등에서의 상호 작용을 높일 수 있다. 감정에 의한 색 변환은 콘텐츠 감상에 있어서 사용자에게 콘텐츠와 자신이 교감하고 있다는 느낌을 주어 흥미와 몰입을 높일 수 있다. 이에 따라, 본 논문은 감정 기반 색 변환을 위한 각 감정 별 색의 범위를 정의하고, 색상, 채도, 명도에 대한 색 변환을 수행한다. 하지만 색과 감정의 관계는 개인의 취향, 환경 등에 따라 다양하게 정의 된다. 이러한 특성을 반영하기 위해, 사용자 취향을 학습함으로써 사용자의 만족도를 높인다. 취향 학습 방법으로는 대화형 유전자 알고리즘을 사용한다. 사용자 취향에 의한 색변환 영상의 만족도를 분석하기 위해 설문조사를 실시하여, 원본 영상에 비해 색변환 영상에 대한 선호도가 높음을 확인한다. 이를 통해 템플릿을 동일하게 적용하는 것보다 사용자 취향을 반영한 템플릿에 의한 변환이 사용자의 감성을 만족시킴을 보인다.

• Journal of Plant Biotechnology
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• 제4권3호
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• pp.91-94
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• 2002

An efficient molecular breeding technique for coffee plants was developed. In order to produce transgenic coffee plants, we established a model transformation procedure via Agrobacterium method. We isolated a gene encoding a protein possessing 7-methylxanthine methyltransferase (theobromine synthase) activity, and it was designated as Coffea arabica 7-methylxanthine methyl transferase; CaMXMT. Using this clone, we produced transgenic coffee plants, in which the expression of CaMXMT is suppressed by double-stranded RNA interference (RNAi) andlor anti-sense methods. The expression pattern of CaMXMT was analyzed by reverse transcription-PCR method and we found that, in the transformed cell lines, the level of transcripts were obviously suppressed by RNAi. The endogenous level of caffeine in the transformed cells was dramatically reduced in comparison with non-transformed cells.