• Animal Bioscience
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• 제36권1호
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• pp.29-42
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• 2023

Objective: Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes. Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs. Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (CDK8) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (YBX1) was a crucial transcription factor for CDK8, which mediated the effect of CDK8 in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs. Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. CDK8 was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.224.1-224
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• 1996

No Abstract, See Full Text

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2004년도 춘계학술대회
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• pp.25-26
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• 2004

• 한국독성학회:학술대회논문집
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• 한국독성학회 2004년도 춘계학술대회
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• pp.25-26
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• 2004

• International Journal of Precision Engineering and Manufacturing
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• 제1권1호
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• pp.62-70
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• 2000

The design of gear train is a kind of mixed problems which have to determine various types of design variables; i,e., continuous, discrete, and integer variables. Therefore, the most common practice of optimum design using the derivative of objective function has difficulty in solving those kinds of problems and the optimum solution also depends on initial guess because there are many sophisticated constrains. In this study, the Genetic Algorithm is introduced for the optimum design of gear trains to solve such problems and we propose a genetic algorithm based gear design system. This system is applied for the geometrical volume(size) minimization problem of the two-stage gear train and the simple planetary gear train to show that genetic algorithm is better than the conventional algorithm solving the problems that have continuous, discrete, and integer variables. In this system, each design factor such as strength, durability, interference, contact ratio, etc. is considered on the basis of AGMA standards to satisfy the required design specification and the performance with minimizing the geometrical volume(size) of gear trains

• BMB Reports
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• 제48권8호
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• pp.473-478
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• 2015

The NEK6 (NIMA-related kinases 6) is reported to play po-tential roles in tumorigenesis. Although it is suggested to function in several cellular pathways, the underlying mechanism in tumorigenesis is still largely unknown. In the present study, we discovered interaction of NEK6 with Smad4, a key member of transforming growth factor beta (TGFβ) pathway. Over-expression of NEK6 in hepatocellular carcinoma (HCC) cell lines suppresses TGFβ-mediated transcription activity in a kinase activity-dependent manner. In addition, NEK6 suppresses the cell growth arrest induced by TGFβ. Mechanically, NEK6 blocks nuclear translocation of Smad4, which is essential for TGFβ function. Moreover, we identified that NEK6 could be regulated by TGFβ and hypoxia. Our study sheds new light on the roles of NEK6 in canonical TGFβ/Smad pathway and tum-origenesis. [BMB Reports 2015; 48(8): 473-478]

• 한국정보처리학회논문지
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• 제6권12호
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• pp.3703-3709
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• 1999

본 논문에서는 영상의 밝기 정보로부터 물체의 표면 형상을 재구성하는 새로운 접근 방법을 제시한다. 표면 재구성 문제를 최적화 문제로 정의하고 국지 해(local minima)에 빠지기 쉬운 기존의 경사법(gradient method) 대신 유전자 알고리즘(genetic algorithm)을 사용한다. 이를 위해 2차원 이미지에 적절한 유전자 표현 및 유전자 연산을 제시한다. 또한 입력 이미지를 단계별로 축소하고, 축소된 이미지에 유전자 알고리즘을 적용하여 큰 형상을 먼저 결정한 후 미세한 형상을 찾아내는 계층적 방법을 적용함으로써 유전자 알고리즘의 수렴 속도를 개선한다. 반사 모델로 기존의 람버션 반사 모델(Lambertian illumination model)에 거리 요소를 포함시켜 보다 현실과 비슷한 제약 조건을 주었으며 실험을 통해 제시된 방법의 타당성을 보인다.

• 한국소음진동공학회논문집
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• 제25권10호
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• pp.677-684
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• 2015

It is often necessary to predict the vibration patterns of the structures from the signals of finite number of vibration sensors. This study presents the optimal placement of vibration sensors by applying the genetic algorithm and the modal expansion method. The modal expansion method is used to estimate the vibration response of the whole structure. The genetic algorithm is used to estimate the optimal placement of vibration sensors. Optimal sensor placement can be obtained so that the fitness function is minimized in the genetic algorithm. This paper discusses the comparison of the performances of two types of fitness functions, modal assurance criteria(MAC) and condition number( CN). As a result, the estimation using MAC shows better performance than using CN.

• Structural Engineering and Mechanics
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• 제68권5호
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• pp.549-561
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• 2018

Sobol method is applied as a powerful variance decomposition technique in the field of global sensitivity analysis (GSA). The paper is devoted to increase convergence speed of the extracted Sobol indices using a new proposed sampling technique called genetic based Latine hypercube sampling (GBLHS). This technique is indeed an improved version of restricted Latine hypercube sampling (LHS) and the optimization algorithm is inspired from genetic algorithm in a new approach. The new approach is based on the optimization of minimax value of LHS arrays using manipulation of array indices as chromosomes in genetic algorithm. The improved Sobol method is implemented to perform factor prioritization and fixing of an uncertain comprehensive high speed rotor-bearing system. The finite element method is employed for rotor-bearing modeling by considering Eshleman-Eubanks assumption and interaction of axial force on the rotor whirling behavior. The performance of the GBLHS technique are compared with the Monte Carlo Simulation (MCS), LHS and Optimized LHS (Minimax. criteria). Comparison of the GBLHS with other techniques demonstrates its capability for increasing convergence speed of the sensitivity indices and improving computational time of the GSA.

• Mycobiology
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• 제47권1호
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• pp.76-86
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• 2019

Scab disease caused by Venturia nashicola is of agroeconomic importance in cultivation of Asian pear. However, little is known about the degree of genetic diversity in the populations of this pathogen. In this study, we collected 55 isolates from pear scab lesions in 13 major cultivation areas in Korea and examined the diversity using sequences of internal transcribed spacer (ITS) region, ${\beta}$-tubulin (TUB2), and translation elongation factor-$1{\alpha}$ ($TEF-1{\alpha}$) genes as molecular markers. Despite a low level of overall sequence variation, we found three distinctive subgroups from phylogenetic analysis of combined ITS, TUB2, and $TEF-1{\alpha}$ sequences. Among the three subgroups, subgroup 1 (60% of isolates collected) was predominant compared to subgroup 2 (23.6%) or subgroup 3 (16.4%) and was distributed throughout Korea. To understand the genetic diversity among the subgroups, RAPD analysis was performed. The isolates yielded highly diverse amplicon patterns and none of the defined subgroups within the dendrogram were supported by bootstrap values greater than 30%. Moreover, there is no significant correlation between the geographical distribution and the subgroups defined by molecular phylogeny. Our data suggest a low level of genetic diversification among the populations of V. nashicola in Korea.