• Journal of Microbiology and Biotechnology
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• 제19권8호
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• pp.749-759
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• 2009

A survey of Bacillus thuringiensis (Berliner) strains isolated from Spanish citrus orchards has been performed, and the strains were tested for insecticidal activity against the Mediterranean fruit fly Ceratitis capitata (Wiedemann), a key citrus pest in Spain. From a total of 150 environmental samples, 376 isolates were selected, recording a total B. thuringiensis index of 0.52. The collection was characterized by means of phase-contrast microscopy, SDS-PAGE, and PCR analysis with primer pairs detecting toxin genes cry1, cry2, cry3, cry4, cry5, cry7, cry8, cry9, cry10, cry11, cry12, cry14, cry17, cry19, cry21, cry27, cry39, cry44, cyt1, and cyt2. Diverse crystal inclusion morphologies were identified: bipyramidal (45%), round (40%), adhered to the spore (7%), small (5%), and irregular (3%). SDS-PAGE of spore-crystal preparations revealed 39 different electrophoresis patterns. All primer pairs used in PCR tests gave positive amplifications in strains of our collection, except for primers for detection of cry3, cry19, cry39, or cry44 genes. Strains containing cry1, cry2, cry4, and cry27 genes were the most abundant (48.7%, 46%, 11.2%, and 8.2% of the strains, respectively). Ten different genetic profiles were found, although a total of 109 strains did not amplify with the set of primers used. Screening for toxicity against C. capitata adults was performed using both spore-crystal and soluble fractions. Mortality levels were less than 30%. We have developed a large and diverse B. thuringiensis strain collection with huge potential to control several agricultural pests; however, further research is needed to find out Bt strains active against C. capitata.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.82-87
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• 2001

A feeding trial was conducted to evaluate the effects of replacing fish meal (FM) with ensiled shrimp by-product (ESB) in a cassava root meal and rice bran-based diet on the performance and carcass characteristics of growing pigs. Thirty six crossbred (Large White$\times$Mong Cai) pigs, with an average initial BW of 19.4 kg, were randomly allocated to one of three different dietary treatments in which the crude protein of the FM was replaced with 0, 50 or 100% ESB. The animals were fed restrictedly and at the end of the experiment at a BW of about 90 kg, 6 representative animals in each treatment group were slaughtered for carcass quality evaluation. Animal growth performance and daily feed intake were significantly reduced (p<0.05) by the inclusion of shrimp by-products in the diets, whereas feed conversion ratios and carcass measurements were not significantly affected (p>0.05). Daily weight gains of the pigs fed the 100% FM diet and 50% ESB diet were significantly (p<0.05) higher than those of pigs fed the 100% ESB diet. In conclusion, from an economical as well as performance point of view, ESB can replace 50% of the crude protein of FM in cassava root meal and rice bran-based diets for growing pigs with a low genetic growth potential.

• Journal of Microbiology and Biotechnology
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• 제19권3호
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• pp.250-256
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• 2009

Acinetobacter strain $KNF2022^T$ was isolated from tobacco plant roots during the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) and examined by phenotypic, chemotaxonomic, and genetic characterization. It was a nonmotile, Gram-negative bacterium. This strain contained Q-9 as the main respiratory quinone. The major cellular fatty acids of the isolate were 16:0, 18:1 w9c, and 16:1 w7c/15 iso 2OH. The DNA base composition was 44 mol%. Phylogenetic analysis based on the 16S rRNA sequence revealed that the isolate formed an evolutionary lineage distinct from other Acinetobacter species. Based on the evaluation of morphologic, physiologic, and chemotaxonomic characteristics, DNA-DNA hybridization values, and 16S rRNA sequence comparison, we propose the new species Acinetobacter antiviralis sp. nov., the type strain of which is $KNF2022^T$ (=KCTC $0699BP^T$).

• Journal of Animal Science and Technology
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• 제49권2호
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• pp.195-202
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• 2007

돼지의 중요한 경제형질인 성장형질은 검정기간동안의 증체량이나 특정한 체중에 도달하는 일수를 측정한다. 그러나 성장형질은 개체의 능력외의 다른 요인에 의해서도 영향을 받는다. 유전능력 평가를 위해서는 합리적인 측정치를 이용하여 불필요한 오류를 줄여야 한다. 이러한 요인에 의한 성장률의 차이를 줄이기 위하여 경남첨단양돈연구소의 경남흑돈(Berkshire) 1,576두의 자료를 가지고 성장곡선을 추정하여 성장곡선에 의해 일당증체량과 90kg 도달일령을 각각 추정하였다. 기존의 방법들과 정확도를 직접적으로 비교할 수 없으나 추정하는 방법론에 있어서 더 정교하여졌다. 이론적 우수성은 일당 증체량에서는 성장곡선상의 같은 연령을 비교하고, 90kg 도달일령에서는 종료체중 측정일에서 90kg 도달일까지의 일수를 개체의 성장곡선상의 일 증체량으로 계산하는 데에 있다. 또한 컴퓨터를 활용한다면 품종이나 성과 같은 요인들에 대해서도 이 추정방법을 쉽게 각각 적용할 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1247-1253
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• 2014

Mutations in the DNMT3A and IDH genes represent the most common genetic alteration after FLT3/NPM1 in acute myeloid leukemia (AML). We here analyzed the frequency and distribution pattern of DNMT3A and IDH mutations and their associations with other molecular markers in normal karyotype AML patients. Fortyfive patients were screened for mutations in DNMT3A (R882), IDH1 (R132) and IDH2 (R140 and R172) genes by direct sequencing. Of the 45 patients screened, DNMT3A and IDH mutations were observed in 6 (13.3%) and 7 (15.4%), respectively. Patients with isolated DNMT3A mutations were seen in 4 cases (9%), isolated IDH mutations in 5 (11.1%), while interestingly, two cases showed both DNMT3A and IDH mutations (4.3%). Nucleotide sequencing of DNMT3A revealed missense mutations (R882H and R882C), while that of IDH revealed R172K, R140Q, R132H and R132S. Both DNMT3A and IDH mutations were observed only in adults, with a higher frequency in males. DNMT3A and IDH mutations were significantly associated with NPM1, while trends towards higher coexistence with FLT3 mutations were observed. This is the first study to evaluate DNMT3A/IDH mutations in Indian patients. Significant associations among the various molecular markers was observed, that highlights cooperation between them and possible roles in improved risk stratification.

• 대한한의학회지
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• 제41권2호
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• pp.80-95
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• 2020

Objectives: The purpose of this study is to assess the quality of the Journal of Korean medicine case reports. Methods: Case reports published in the Journal of Korean Medicine from January 2015 to March 2020 were selected by using Oriental Medicine Advanced Searching Integrated System (OASIS) and the journal search system of the Korean Medical Association (https://www.jkom.org). The quality of the case reports was assessed using the Consensus-based Clinical Case Reporting Guideline Development (CARE) guidelines. Results: 33 case reports were selected for the assessment. Based on the CARE guidelines, 61.54% of the case reports included necessary information, but the quality level was uneven. More than 60% of the reports were missing data regarding 'Discussion of the strengths and limitations in your approach to this case', 'Intervention adherence and tolerability', 'Timeline', 'Medical, family, and psychosocial history including relevant genetic information', 'Patient perspective or experience', 'Adverse and unanticipated events', 'Administration of intervention', and 'De-identified demographic information and other patient specific information'. In most reports of over 90%, data regarding 'Diagnostic challenges', 'Intervention adherence and tolerability', and 'Key word' were not included. Conclusions: Efforts are needed to improve the quality of case reports in the Journal of Korean Medicine, and it is necessary to develop appropriate guidelines for case reporting for the Journal of Korean Medicine. In addition, all articles submitted to the Journal of Korean Medicine are to be complied with submission instructions and CARE guidelines.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
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• pp.146-150
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• 2007

인터넷이 생활의 일부가 되어가면서 정보의 양도 급속도로 늘어나고 있으며, 이로 인해 많은 데이터 속에서 정보를 찾아내는 기술이 부각되고 있다. 협력적 필터링은 유사한 선호도를 기반으로 관심을 가질 것으로 생각되는 아이템을 추천하는 방법이다. 그러나 비슷한 선호도를 가진 일부 사용자의 정보를 바탕으로 하기 때문에 나머지 사용자의 정보를 무시하는 경향이 있다. 본 논문에서는 여학생의 선호도를 이용한 감성공학적 의상 코디를 제안한다. 이는 유전자 알고리즘에 의한 적합함수로 평가값을 계산하고 a-cut을 이용하여 사용자를 군집한다. 마지막으로 협력적 필터링에 의해 의상 코디를 추천한다. 성능평가를 위해 설문조사 데이터 집합에서 FAIMS-I, FAIMS-II과 비교 평가하였다.

• 한국멀티미디어학회논문지
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• 제12권2호
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• pp.290-299
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• 2009

진화하는 인공신경망은 인공지능분야와 게임 NPC의 지능 설계 분야에서 새롭게 각광을 받고 있다. 하지만 진화하는 인공신경 망을 이용하여 게임 NPC의 지능을 설계할 때 인공신경 망의 구조가 복잡함에 따라 진화와 평가에 필요한 연산량이 크며 또한 적절한 적합도 함수를 설계하지 못하면 지능적인 NPC를 설계할 수 없는 등의 문제점을 가지고 있다. 본 논문에서는 이러한 문제들을 해결하고자 동적 상태 진화 인공신경망을 제안한다. 동적 상태 진화 인공신경망은 전통적인 진화하는 인공신경망 알고리즘에 기반하여 진화 과정에서 신경망의 신경세포들 사이의 시냅스를 제거(disabled) 하거나 고정(fixed)시키는 방법을 통하여 진화와 평가과정에 소모되는 연산량을 줄이는 알고리즘이다. 본 논문은 Darwin Platform 을 테스트 베드로 축구게임 NPC의 지능 설계를 통하여 제안하는 방법의 유용성을 검증한다.

• 한국수산과학회지
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• 제53권3호
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• pp.297-307
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• 2020

This study investigated the cosmetic effects of enzymatic hydrolytes of an aquatic by-product, fish skin. The skins of olive flounder Paralichthys olivaceus (PO) and Alaska pollock Gadus chalcogrammus (AP) were hydrolyzed using pepsin, Alcalase, and Protemax. Three enzymatic hydrolytes were obtained and the inhibitory effects of these hydrolytes on the aging-related enzymes tyrosinase, elastase, and collagenase were determined. The results indicated that the pepsin hydrolytes of PO and PA had stronger activities than the other hydrolytes. PO and PA also significantly reduced the intracellular reactive oxygen species levels in and improved the viability of H₂O₂-treated Vero cells; decreased nitric oxide production by and increased the cell viability of lipopolysaccharide-treated RAW 264.7 cells; and reduced intracellular reactive oxygen species levels and improved the viability of ultraviolet B irradiated HaCaT cells and human dermal fibroblasts. Furthermore, PO and PA remarkably reduced the intra- and extracellular melanin contents of alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. These results demonstrate that PO and PA have potential for use in the cosmetic industry.

• 대한조선학회논문집
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• 제39권1호
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• pp.73-81
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• 2002

일반적으로, 선박에 탑재되는 장비의 진동문제는 장비자체의 기진력에 의한 진동문제뿐만 아니라 외부기진력인 주기관과 추진기의 기진력이 장비하부 데크를 통하여 장비에 과도 진동을 유발하는 경우가 많다. 따라서 본 연구에서는, 선박에 탑재되는 장비 마운팅 시스템의 진동 성능을 평가할 수 있도록 고유진동해석, 전달율계산, 강제진동해석 등을 수행할 수 있는 프로그램을 개발하였고, 선내 주요기진원과 장비의 공진시 공진회피를 위한 마운트 설계 변경 프로그램과, 그리고 장비 마운트 하부데크의 진동에 의한 장비의 무게중심에서 속도응답이 최소가 되도록, 마운트 강성을 결정하는 최적화 프로그램을 개발하였다.