• The Plant Pathology Journal
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• 제22권3호
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• pp.260-264
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• 2006

Talaromyces flavus mediates the transmission of Apple stem grooving virus (ASGV) to several host plants. The ASGV-F carried by T.flavus was partially purified from the fungus. Based on sequence analysis and homology searches, this is closely related to other ASGV strains isolated from host plants. The partially purified viral coat protein (CP) was separated on a 12% SDS-polyacrylamide gel and analyzed by Western blotting with an ASGV anti-serum. A single band at 28 kDa reacted with the ASGV anti-serum. The deduced amino acid sequence of the ORF-l showed conserved domains, including an NTP-binding helicase motif, GFAGSGKT. The amino acid sequences of the helicase and CP showed strong homology to other ASGV strains (98%). All ASGV isolated from plants and fungi had salt bridges composed of the CP and the GFAGSGKT motif of the helicase, which are commonly conserved in plant viruses. These results suggest that ASGV-F is one of ASGV strains isolated from T.flavus based on sequence similarity as well as the serological analysis of CP.

• The Plant Pathology Journal
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• 제22권3호
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• pp.248-254
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• 2006

Pear black necrotic leaf spot (PBNLS) on pear trees (Pyrus pyrifolia) is caused by a Korean isolate of Apple stem grooving virus (ASGV-K). Yellow spots were detected in Phaseolus vulgaris (kidney bean) and Chenopodium quinoa which were grown near the diseased pears in year 2000 through 2003. The ASGV-K, the causative agent of PBNLS, was detected from the symptoms of the diseased kidney bean plant and C. quinoa. ASGV-harboring fungi were also isolated from symptomatic plants and from soils surrounding the infected plants. The ASGV-harboring fungus was identified and characterized as Talaromyces flavus. Ecopathological studies showed that the number of ASGV-harboring fungi on the pear leaves was not correlated with differences in temperature or severity of symptoms. Additionally, there was no difference in fungus frequency among the orchard locations or different host plants. Although the frequency of fungi isolated from the soil was not affected by changes in temperature or location, the fungi occurred at higher densities in the rhizosphere than in the plants themselves.

• The Plant Pathology Journal
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• 제22권3호
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• pp.255-259
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• 2006

The pathogenicity to pear trees and other experimental hosts of the Apple stem grooving virus Korean isolate (ASGV-K) carried by a fungal vector, Talaromyces flavus was examined. ASGV-harboring T. flavus induced mild symptoms on virus-free pears. Symptom severity was intermediate between pears showing typical PBNLS and virus-free pears. Ten cultivars of Phaseolus vulgaris showed 35%-90% infectivity by direct infiltration into leaves and roots by ASGV-harboring T. flavus. Application of fungal cultures to soils showed 0%-70% infectivity depending on the P. vulgaris cultivar. Sap extracted from ASGV-infected Chenopodium quinoa induced similar symptoms on P. vulgaris at 25 days after inoculation. Similar symptoms were also detected on P. vulgaris which were inoculated with ASGV-harboring T.flavus. When healthy P. vulgaris leaves were challenged with sap extracted from P. vulgaris leaves infected with ASGV-harboring T. flavus, typical symptoms were observed. These data suggest that T. flavus mediates the transfer of ASGV to host plants.

• 전자공학회논문지CI
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• 제48권2호
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• pp.47-54
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• 2011

본 논문에서는 한 장의 이미지에서 학습을 통하여 영역 별 깊이 정보를 추정할 때 사용되는 특징 정보를 유전 알고리즘(Genetic Algorithm)을 기반으로 축소하고 깊이 정보 추정 시간을 단축하는 방법에 대해서 기술 한다. 깊이 정보는 이미지의 에너지 값과 텍스쳐의 기울기 등을 특징으로 생성하여 특징들의 관계를 기반으로 추정 된다. 이 때 사용되는 특징의 차원이 크기 때문에 연산시간이 증가하고 특징의 중요성을 판단하지 않고 사용하여 오히려 성능에 나쁜 영향을 미치기도 한다. 이에 따라 중요성을 판단하여 특징의 차원을 줄일 필요가 있다. 본 논문에서 제안한 방법을 미국 스탠포드(Stanford)대학에서 제공하는 벤치마크 데이터로 실험한 결과, 특징의 추출과 깊이 추정 연산 시간이 모든 특징을 사용하는 방법에 비하여 약 60%정도 향상되고 정확도가 평균 0.4%에서 최대 2.5% 향상 되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1536-1540
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• 2006

Crossbreeding effects for wool quality traits viz. greasy fleece weight (kg), staple length (cm), average fibre diameter (${\mu}$) and medulation percentage were estimated using the Dickerson's and Kinghorn's models. The data analyzed involved 15 genetic groups including Nali purebred, $F_1$'s of two and three breeds, $F_2$'s and reciprocal crossbred obtained from the crossing of Nali (N), Merino (M) and Corriedale (C) breeds during 1980-96. Nali and Corriedale breeds had non-significant negative additive genetic effects (Dickerson's model) on greasy fleece weight, while effects of Corriedale were negative for staple length only from both models. In general additive genetic effects of all three breeds were non-significant for all the wool traits except medulation percentage. Non significant heterotic and recombination effects (epistatic loss) were estimated from both models. However, the estimates of crossbreeding effects varied between the models both in magnitude as well as in direction barring few exceptions. Undesirable positive heterosis was found on medulation percentage for all types of combinations involving three breeds. Comparison of least squares means of various genetic groups revealed that both two breed and three breed crosses were superior to the Nali breed for all wool quality traits. Fibre diameter of MN crossbreds was significantly less than CN crossbreds. Results also indicated that as the inheritance of Nali breed in a cross is decreased, the medulation percentage decreases which is desirable. Inter se mating of crossbreds (two breed, three breed) has not resulted in a decline in the wool quality traits. These results indicate that the synthetic population derived from three breeds can be stabilized easily for wool traits as there may not be epistatic loss on subsequent inter se mating of crossbreds.

• Journal of the Korean Data and Information Science Society
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• 제28권5호
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• pp.1043-1053
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• 2017

본 연구는 산양 7 품종을 대상으로 (Saanen (88), Laoshan (67), Toggenburg (32), Alpine (12), Anglonubian (9), Jamnapari (7), Black Bengal (4)) 13종의 초위성체 마커 (microsatellite marker)를 활용하여 유전적 다형성 분석을 실시하였다. 대립유전자 수는 4개 (INRA005) 부터 18개 (SRCRSP23)까지 확인되었으며, 관측이형접합율 ($H_{obs}$)과 기대이형접합율 ($H_{\exp}$) 그리고 다형성 정보지수 (PIC) 값은 각각 0.482 ~ 0.786, 0.476 ~ 0.923 그리고 0.392 ~ 0.915로 나타났다. 품종별 유전적 거리를 확인하기 위하여 실시한 주성분분석 (PCoA) 결과는 요인대응분석 (FCA) 분석과 유사한 결과를 보였으며, 동일개체출현빈도는 $2.47{\times}10^{-15}$으로 확인되었다. 따라서 본 연구 결과는 산양 품종 개량 및 보존에 있어 기초자료로써 유용한 자료로 활용 가능 할 것으로 사료된다.

• The Plant Pathology Journal
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• 제22권1호
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• pp.68-74
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• 2006

A new virus with a dsRNA genome was isolated and characterized from the Suhan-:neutari strain (ASI 2504) of Pleurotus ostreatus, which was characterized as long and slightly bent with small caps on the stipe of fruit body. Thirty nm isometric viruses with three dsRNA segments (approximately 2.0, 1.84 and 1.82 kb in sizes) were isolated by ultracentrifugation in sucrose gradients. Western analysis of protein extracted purified viruses with anti-virus polyclonal antibody confirmed that viruses have two specific proteins (36 and 68 kDa). Computer analysis of 2.0 kb segment shows that high. sequence identity with RNA-dependent RNA polymerase (RdRp) of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis, OMDV was most related to Pleurotus ostreatus virus 1.

• 한국정보통신학회논문지
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• 제15권12호
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• pp.2687-2696
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• 2011

해양 센서 네트워크에서 클러스터링 문제는 네트워크 수명 및 확장성을 향상시키기 위한 문제 중 하나이다. 본 논문은 모든 노드가 클러스터 헤더를 통하여 싱크 노드로 데이터를 전송하는데 사용되는 전송 에너지를 최소화할 수 있는 클러스터링 문제의 최적 결과를 찾는 알고리즘을 제안한다. 일반적으로 네트워크에서 노드의 수가 증가하면 적절한 클러스터 구성을 위한 계산량은 급격히 늘어나게 된다. 본 논문에서는 적정한 시간 내에 최적의 클러스터 구성을 위한 유전 알고리즘을 제안하며, 효율적인 검색을 위해 유전 알고리즘의 효과적인 이웃해 생성 동작을 제안한다. 제안된 알고리즘은 전송 에너지와 실행시간 관점에서 성능을 평가하며, 평가 결과에서 제안된 알고리즘이 해양 센서 네트워크에서 클러스터를 효과적으로 구성함을 보인다.

• 한국작물학회지
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• 제51권2호
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• pp.163-168
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• 2006

Improvement of crop yield can be achieved through understanding genetic variation in reproductive characters and its impact on yield components. The present study was performed to evaluate genetic diversity for reproductive growth characters in exotic germplasm resources and to determine the relationships between developmental and growth periods with yield and yield components in soybean cultivar groups. For phenotypic evaluation such as reproductive and agronomic traits, a total of 80 indigenous and exotic soybean cultivars collected from four different geographical regions (China, Japan, Korea, and Vietnam) were grown from May to November of 2003 at the Seoul National University Farm, Suwon, Korea ($127^{\circ}02'E$ longitude, $37^{\circ}26'N$ latitude). Most of all the characters exhibited wide range of phenotypic variation, of which pod number, seed number, and plant yield showed greater range as compared to other characters. Korean cultivar groups showed greater diversity than the other cultivar groups in seven characters. Correlation analysis showed that days to flowering (DTF) and days to maturity (DTM) had close association with agronomic traits as well as yield and yield components. Both DTF and DTM had positive correlation with the other characters except one hundred seed weight. Stepwise multiple linear regression revealed that seed and pod number were identified as being significant for plant yield. The results in this study indicated wide variation in agronomic traits including DTF and DTM, suggesting the valuable genetic resources in a soybean breeding program.

• Environmental Engineering Research
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• 제11권5호
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• pp.277-284
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• 2006

The genetic toxicity of environmental pollutants, namely, nonylphenol (NP), bisphenol A (BPA) and chloropyriphos (CP) was investigated in aquatic sentinel species, freshwater crustacean, Daphnia magna, and larva of aquatic midge, Chironomus tentans, using Comet assay. Physiological effect of such pollutants was also investigated by studying the specimens' rates of reproduction, growth and survival. Acute toxicity results showed that, as expected, Daphnia was more sensitive than Chironomus to chemical exposure. The order of acute toxicity was CP > NP > BPA in D. magna and NP > CP > BPA in C. tentans. BPA may exert a genotoxic effect on D. magna and C. tentans, given that DNA strand breaks increased in both species exposed to this compound, whereas NP- and CP-induced DNA damage occurred only in C. tentans. In vivo genotoxic data obtained in aquatic sentinel species could provide valuable information for freshwater quality monitoring. The experiments with NP-exposed D. magna showed that the pollutant has long-term effects on reproduction, whereas no short-term effect on DNA integrity was found, being an example of a false-negative result from the biomarkers perspective. This result could be interpreted that other mechanism than genetic alteration might be involved in NP-induced reproduction failure in D. magna. False-positive results from the genotoxic biomarker obtained in BPA-exposed D. magna and in NP-exposed C. tentans make it difficult to use DNA integrity as an early warning biomarker. However, as the mere presence of genotoxic compounds, which are potentially carcinogenic, is of high concern to human and ecosystem health, it could also be important to rapidly and effectively detect genotoxic compounds in the aquatic system in ways that do not necessarily accompany a higher level of alteration. Considering the potential of D. magna and C. tentans as bioindicator species, and the importance of genotoxic biomarkers in ecotoxicity monitoring, DNA damage in these species could provide useful information for environmental risk assessment.