• Journal of the Korean Data and Information Science Society
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• 제21권4호
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• pp.795-802
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• 2010

This study defined the genetic diversity of five breeds of cattle in Asia by analyzing 6 microsatellite markers in 270 animals. Based on expected mean heterozygosity, the lowest genetic diversity was exhibited in Japanese black cattle (HE=0.5849), and the highest in Chinese yellow cattle (HE=0.8073). Average proportion of genetic variation due to interpopulation subdivision among these five cattle breeds varied between 11.7 and 12.5%. The genetic distances were roughly divided into three groups: Japanese black cattle, Holstein, and the three remaining breeds. This clustering agrees with the origin and geographical distributions of these five cattle breeds.

• Animal cells and systems
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• 제5권2호
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• pp.101-106
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• 2001

AFLPS (amplified fragment length polymorphisms) were used to estimate the genetic diversity of seven populations of Brassica campestis L. ssp. napus var. nippo-oleifera Makino between naturalized and cultivated populations. The seven Korean populations maintained a high level of genetic diversity. For example, all eight primers were high polymorphic, with an average of 3.2 effective alleles per primer set, and the expected heterozygosity was also high. The majority of genetic variance resided within populations The combinations of an insect-pollinated, outcrossing breeding system, large populations sizes, a high degree of gene flow and a propensity for high fecundity may explain the high level of genetic diversity within cultivated populations. Estimates of genetic similarity on the proportion of shared fragments ranged from 0.952 to 0.999. The high level of gene flow In Korean naturalized populations is mainly caused by seed dispersal via sea tide and the gene flow of cultivated populations may be enhanced in part by artificial pollen dispersal.

• Journal of Plant Biotechnology
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• 제44권3호
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• pp.312-319
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• 2017

The need to preserve and use plant genetic resources is widely recognized, and the prospect of dwindling plant genetic diversity, coupled with increased demands on these resources, has made them a topic of global discussion. In the present study, the genetic diversity and population structure of 73 ginseng accessions collected from six regions in China were analyzed using eight simple sequence repeat (SSR) markers. Major allele frequencies ranged between 0.38 ~ 0.78, with a mean allele frequency value of 0.571. The number of alleles discovered ranged from 3 to 10 per accession, with a mean number of 7; 56 alleles were discovered in total. Gene diversity (GD) and polymorphic information content (PIC) values were similar to each other, and they ranged from 0.36 ~ 0.77 (mean 0.588) and 0.33 ~ 0.74 (mean 0.548), respectively. Accessions were divided into three clusters based on their phylogenetic relationships and genetic similarities, and although the populations were similar, they were not classified according to the region. Regional genetic diversity was also similar, with slight differences observed based on the number of accessions per region. It is expected that the findings of the present study can provide basic data for future studies on ginseng genetic diversity and for breeding ginseng cultivars.

• 생명과학회지
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• 제20권6호
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• pp.819-824
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• 2010

왕대속 대나무들은 대부분 동남아시아에 분포한다. 전세계적으로 왕대속에 속하는 4종은 의학적, 생태학적으로 중요시 되어 왔다. 이번 연구에서 우리나라에 자생하고 있는 왕대속 4종을 RAPD마커를 이용하여 유전적 관계 분석하였다. RAPD분석결과 왕대속에 속하는 4종의 대나무는 명확하게 분류가 되었고 8.9~33.3%로 다형현상이 나타났다. 특히 왕대는 다른 종들 보다 유전적 다양성이 0.018로 가장 낮게 나왔다. 그리고 집단 내 유전적 다양성(Hs)은 0.315, 집단간 다양성(Gst)은 0.659 그리고 유전자 유동(Nm)은 0.0263로 나타났다. 이는 한국의 왕대속 집단은 지리적 및 환경적 요인을 받아 유전적 다양성이 낮게 나타났으며 본 연구는 대나무 유전적 다양성 연구에 중요한 기초자료가 될 것으로 사료된다.

• BMB Reports
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• 제46권8호
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• pp.416-421
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• 2013

Genome-wide association studies (GWAS) have become popular as an approach for the identification of large numbers of phenotype-associated variants. However, differences in genetic architecture and environmental factors mean that the effect of variants can vary across populations. Understanding population genetic diversity is valuable for the investigation of possible population specific and independent effects of variants. EvoSNP-DB aims to provide information regarding genetic diversity among East Asian populations, including Chinese, Japanese, and Korean. Non-redundant SNPs (1.6 million) were genotyped in 54 Korean trios (162 samples) and were compared with 4 million SNPs from HapMap phase II populations. EvoSNP-DB provides two user interfaces for data query and visualization, and integrates scores of genetic diversity (Fst and VarLD) at the level of SNPs, genes, and chromosome regions. EvoSNP-DB is a web-based application that allows users to navigate and visualize measurements of population genetic differences in an interactive manner, and is available online at [http://biomi.cdc.go.kr/EvoSNP/].

• 한국약용작물학회지
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• 제21권3호
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• pp.220-228
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• 2013

This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.

• 한국작물학회지
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• 제65권4호
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• pp.447-456
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• 2020

Peanut is an allotetraploid derived from a single recent polyploidization. Polyploidization has been reported to have caused significant loss in genetic diversity during the domestication of cultivated peanuts. Single nucleotide polymorphism (SNP)-based markers such as cleaved amplified polymorphic sequences (CAPS) derived from next-generation sequencing (NGS) have been developed and widely applied for breeding and genetic research in peanuts. This study aimed to identify the genetic diversity and population structure using 30 CAPS markers and 96 peanut accessions from five different origins. High genetic dissimilarities were detected between the accessions from Korea and those from the other three South American origins generally regarded as the origin of peanuts, while the accessions from Brazil and Argentina presented the lowest genetic dissimilarity. Based on the results of the present study, accessions from Korea have unique genetic variation compared to those from other countries, while accessions from the other four origins are closely related. Our study identified the genetic differentiation in 96 peanut accessions from five different origins, and this study also showed the successful application of SNP information derived from re-sequencing based on NGS technology.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1631-1635
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• 2007

Japanese Brown cattle, one of the four domestic beef breeds in Japan, are suffering from numerical reduction due to economic pressure from profitable breeds. In this study, all the reproductive cows in the Kouchi sub-breed of the Japanese Brown cattle that were alive in July 2005 were investigated by pedigree analysis to clarify genetic structure and composition of genetic variability. In addition, genetically important individuals for the maintenance of genetic variability of the sub-breed were also identified through the core set method. The number of cows analyzed was 1,349. Their pedigrees were traced back to ancestors born around 1940, and pedigree records of 13,157 animals were used for the analysis. Principal component analysis was performed on the relationship matrix of the cows, and their factor loadings were plotted on a three-dimensional diagram. According to their spatial positions in the diagram, all the cows were subdivided into five genetically distinctive subpopulations of 131 to 437 animals. Genetic diversity of the whole sub-breed, which is estimated to be 0.901, was decomposed into 0.856 and 0.045 of within-subpopulation and between-subpopulation components. Recalculation of genetic diversity after removal of one or several subpopulations from the five subpopulations suggested that three of them were genetically important for the maintenance of genetic variability of the sub-breed. Applying the core set method to all the cows, maximum attainable genetic diversity was estimated to be 0.949, and optimal genetic contributions assigned to each cow supported the previous results indicating relative importance of the three subpopulations as useful genetic materials.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.279-280
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• 2003

Although it has been known though many morphological and physiological studies, its genetic diversity and population structure have not yet been investigated in this species. Therefore, detailed studies, in particular at the DNA level, on genetic diversity of natural populations of wild sea lettuce, and genetic relationships between natural sea lettuce and cultivated sea lettuce are necessary from the viewpoint of plant evolution. (omitted)

• 생명과학회지
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• 제17권7호통권87호
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• pp.877-881
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• 2007

전분 젤 전기영동을 사용하여 한국내 분포하는 세잎양지꽃 8개 집단에서 유전적 다양성과 집단구조를 평가하였다. 종수준에서 효소내 다형현상을 나타내는 대립유전자좌위는 68.4%였다. 집단 수준에서 유전적 다양도는 유사한 생활사를 가진 초본류의 평균값에 비해 높았다. 전체 유전적 다양도는 조사한 8개 집단에 대해 0.190과 0.584사이에 있었으며 평균은 0.371이였다. 집단내 유전적 다양도는 0.354였다. 집단간 분화정도는 비교적 낮았다($G_{ST}$ = 0.065). 고정지수 분석 결과 많은 집단과 대립유전자좌위에서 이형접합체의 결핍이 있었다. 이는 세잎양지꽃은 줄기에서 꽃을 형성하여 종자번식을 하는 타가수분방식과 분지하여 새로운 개체를 형성하는 영양번식을 영위할 수 있는 다양한 번식법을 가지고 있는 클론 식물의 특성에 기인한 것으로 사료된다. 따라서 같은 집단에서 다양한 세대의 존재하여 내교잡(inbreeding)이 발생한 것으로 볼 수 있다.