• Asian-Australasian Journal of Animal Sciences
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• v.27 no.4
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• pp.574-579
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• 2014

A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific activity greater than 500 mg/L and 4,000 U/mg was obtained. Results at optimal pH and temperature showed recombinant lysozyme has higher lytic activity at pH 6.5 and $45^{\circ}C$. This study demonstrates the successful expression of recombinant lysozyme using the eukaryotic host organism P. pastoris paving the way for protein engineering. Additionally, this study shows the feasibility of subsequent industrial manufacture of the enzyme with this expression system together with a high purity scheme for easy high-yield purification.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.637-642
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• 2004

This paper presents a new shape design method for the multi-mode sensor that can detect selected multiple modes for the active vibration control of mechanical structures. The structure used for this study is an isotropic cantilever beam type with a PVDF(polyvinylidene fluoride) which is bonded onto the structure as a sensor. Characteristic behaviors of the sensor are related with the electrode shapes of PVDF. The shape optimization problem is solved by defining a new multi-objective function and using the genetic algorithm. Resulting electrode shape functions have good performances to detect the multiple vibration modes. The results of analytical simulations are compared with those of experiment works. The results agree well each other. Hence, the obtained experimental results give evidence for the validity of the presented theoretical analysis of the electrode shape design problem.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 1999.05a
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• pp.355-360
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• 1999

In this paper, we propose a method that optimizes the parameters of fuzzy logic controller : centers and widths of membership functions and scaling factors using genetic algorithm. Before fuzzy logic controller controls a plant in real time, first off it is optimized by genetic algorithm. We select error and error variation between reference trajectory and real output for the input signals of fuzzy controller. We compared and investigated conventional fuzzy control method and proposed method through simulation and experiment using one link manipulator with nonlinear characteristic.

• Proceedings of the KIEE Conference
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• 1997.11a
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• pp.257-260
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• 1997

This paper designs the optimal PID controller for load frequency control on 2-area power system. Genetic algorithm is utilized to optimize parameters of PID controller which is applied to power system. Using two-point crossover, uniform crossover and one-point crossover, Search performance of genetic algorithm with each crossover method is considered. In case of load variation in 1-area, the dynamic characteristic of power system is considered. The simulation results show that the proposed PID controller is better control performance than PID controller using Ziegler-Nichols method.

• Transactions of the Society of Information Storage Systems
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• v.10 no.1
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• pp.19-22
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• 2014

Optical nonlinear property is utilized to the wave generation, generating the beam at intended wavelength, and optical computing systems. The genetic material, which is the DNA with helical structure in nano-scale, is fascinating for optics communities due to artificially controllable sequence that determines the physical and chemical property. Nonlinearity of DNA was investigated by the four wave mixing experiment, which is with two incident beams located at 1550nm and 1650nm. The four output beams including incident beams are emitted from genetic material such as 1461nm and 1763nm by nonlinear characteristic. The 1461nm beam which is generated by four wave mixing phenomena was observed by optical spectrum analyzer.

• CDE review
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• v.21 no.2
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• pp.39-49
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• 2015

This paper presents the single objective design optimization of plate-fin heat sink equipped with fan cooling system using Genetic Algorithm. The proper heat sink and fan model are selected based on the previous studies. And the thermal resistance of heat sinks and fan efficiency during operation are calculated according to specific design parameters. The objective function is combination of thermal resistance and fan efficiency which have been taken to measure the performance of the heat sink. And Decision making procedure is suggested considering life time of semiconductor and Fan Operating cost. And also Analytical Model used for optimization is validated by Fluent, Ansys 13.0 and this model give a quite reasonable and reliable design.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.32 no.2
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• pp.158-170
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• 2009

This paper considers how the optimal export level is influenced by export risk, the degree of risk-averseness for exporting firms, and those firms' cost structure. In addition, export insurance is incorporated into some simple theoretical model to analyze the optimal export level. This paper applies genetic algorithm simulation to show that the exporting firms'risk-averseness do not affect the optimal export decision while export risk and cost function characteristic have relatively more significant effects on the optimal export level. Finally, our findings suggest that the most influential factor for the optimal export levels seems to be the monopoly power of exporting firms.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1995.10b
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• pp.11-14
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• 1995

A Fuzzy Classifier which deals with very confusing objects is proposed. Naturally this classifier heavily relies on the nulti-feature decision-making procedure. For a simple example, this classifier is applied to the recognition of confusing handwritten numerals 4,6 and 9 The characteristic variables used in this paper are the existence of a loop and the relative location of the starting or ending points(SEP). Thus each sample of handwritten numerals 4, 6 and 9 is classified in one of the 6 groups which are divided according to the sample structure. Each group has its own classifying rules. Also the method of rule-generation using genetic algorithms in each group is proposed.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.52 no.1
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• pp.58-61
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• 2003

A loop power system has a nonlinear characteristics. Also it is very hard to analyse through a equation if a discontinuous characteristic of the ULTC is added to a system. However, the problem which is hard to analyse by equations can acquire the useful result with what use the genetic algorithm (GA) which is a multi-point search program. In this paper, we proved through a simulation that the proposed method can reduce an operation frequency of tap changers and improving the quality of voltage of the buses by decreasing the deviation between the actual voltage and the reference voltage through the coordinated control of the ULTC that use GA in the loop power system.

• Structural Engineering and Mechanics
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• v.25 no.6
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• pp.637-652
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• 2007

This paper presents stacking sequence optimization of laminated angle-ply cylindrical panel based on natural frequency. Finite element method (FEM) is used to obtain the vibration characteristic of an anisotropic panel using the first order shear deformation theory(FSDT) and genetic algorithm (GA) is used to obtain the optimal stacking sequence of the layers. Cylindrical panel has finite length and arbitrary boundary conditions. The thicknesses of the layers are assumed constant and their angles are specified as design variables. The effect of the number of plies and boundary conditions in the fitness function is considered. Numerical examples are presented for four, six and eight layered anisotropic cylindrical panels.