• The Korean Journal of Zoology
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• v.30 no.3
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• pp.211-221
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• 1987

Starch gel electrophoresis was used to estimate the degree of genic variation and genetic relatedness among twelve populations of the Cat-Sanke(Elaphe diene) in Korea. Out of the 17 loci examined 9 loci (Mdh-2, Ldh-1, Ldh-2, Idh-1, Idh-2, Adh, Aco-3, ep, Got-1) were monomorphic with identical mobility in all populations, 4 loci (Pgm, Mpi, Pept-1, Got-2) were nearly identical with minor allele frequency differences, and 4 loci (Mdh-1, Fgi, Est-2, 6psd) showed moderate variation. Levels of genic variation were similar to those found for other reptilian species. The mean genetic similarity value(S) among twelve populations is 0.966. Chechu population was slightly remote genetically from the mainland populations.

• Journal of Microbiology
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• v.34 no.2
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• pp.166-171
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• 1996

Genetic analysis was conducted on newly isolated coliphages form soil by using a RAPD assay. From the initial result, the coliphages were turned out to be different form one another but were closely related to .psi..lambda. due to the fact that they shared the samed RAPD maker in which other T phage testings failed to show. By using the primers EC01 or EC02, a fast genetic mutation of .psi.C1 was found by producing specific RAPD markers on the phages from the first filial progeny to the second filial progeny. When we made a RAPD assay with combined primers (EC01, EC05 and EC08), the genetic mutation was again confirmed in .psi.C1. The assay detection showed mutations in other coliphages such as .psi.C2 and .psi.C3 by revealing specific RAPD bands among different progeny phages, where genetic instability of the coliphages in implied.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.2
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• pp.143-150
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• 1998

Six field bean (GI-vcine soza S and Z ) plants were examined for their genetic polymorphisms and intraspecific variations using randomly amplified polymorphic DNA(RAPD) markers. In RAPD analysis of 5 random primers (Rp-1, Rp2, Rp-3, Rp-4, Rp-5), 30 of total 155 bands obtained kom 5 primers were polymorphic and sizes of polymirphic band ranged between 0.5 and 3.0 kb. Number of bands amplyfied per primer was varied from 2 to 11 and average number was 6.0. Genetic variation of intraspecies in the samples of six region was ranged behveen 11 to 25 percent, and genetic similarity among intraspecies was ranged from 0.69 to 0.78. In pairwise genetic similarity test of six field bean plants, Mun and Hoj showed highest coefficient of genetic similarity as 0.67, whereas Sin and Hoj was lowest as 0.45. According to the genetic similarity, the level of intraspecific variation is higher than that of regional distance in GI-vcine soza.

• Fisheries and Aquatic Sciences
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• v.11 no.1
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• pp.36-40
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• 2008

We analyzed the nucleotide sequences of about 500 bp of the mitochondrial NADH dehydrogenase subunit 3 (ND3) gene to estimate the genetic variation of Korean masu salmon (Oncorhynchus masou) populations. DNA samples were collected from 104 river-only specimens and 52 anadromous specimens from three hatcheries and one river. There are no records of artificial release into the river. We amplified the ND3 gene by polymerase chain reaction, targeting areas that included parts of the cytochrome oxidase III gene and the NADH dehydrogenase subunit 4L gene, and defined 14 haplotypes based on 12 variable nucleotide sites in the examined region. Among the haplotypes, ten were specific to river-only specimens within hatchery populations. Haplotype diversity of river-only populations in hatcheries was higher than that of anadromous and wild populations. Pairwise population $F_{ST}$ estimates and neighbor-joining tree analyses inferred that anadromous and river-only populations were distinct. These results suggest that sequence polymorphism in the ND3 region may be a useful marker for analyzing the genetic variation and population structure of masu salmon.

• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.289-297
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• 2020

The genetic diversity of two subpopulations of Corynandra chelidonii, one of terrestrial and the other of aquatic environments, was measured with molecular markers, such as start codon targeted (SCoT), inter simple sequence repeats (ISSR), and random amplification of polymorphic DNA (RAPD). The traditional morphological traits such as habitat, habit, leaf morphology, the colour of the sepals and petals, number of stamens, and seed morphology formed the base for their realization as two varieties, C. chelidonii var. pallae and C. chelidonii var. chelidonii. The polymorphism between the two variants was 100% with the primers SCoT-2 and OPA-1 and 4, while maximum polymorphism was detected with ISSR-2, SCoT-3, and OPA-3. The study used, for the first time, more than one molecular marker to assess the genetic variation underscoring the morphological variation in Corynandra chelidonii (L.f.) Cochrane & Iltis. The study justifies the recognition of the two subpopulations of Corynandra chelidonii from aquatic and terrestrial environments as two distinct varieties, C. chelidonii var. pallae (Reddy & Raju) V.S.Raju and C. chelidonii var. chelidonii, respectively, based on the traditional taxonomic evidence.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.235-243
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• 2001

Genetic variation of the beet armyworm, Spodoptera exigua (Hubner), was analyzed by polymorphic allozymes. Field populations were subdivided by different hosts, geographical locations, and seasons. Estimated average heterozygosity ($0.443\pm$0.013) indicated high genetic variation in all field populations of S. exigua. There were significant inbreeding effects deviated from Hardy-Weinberg equilibrium in each of subpopulations. These significant nonrandom matings were caused by within-subpopulations probably due to sampling errors, but not by mating isolation among subpopulations. Wrights ($F_{ST}$ ) and Neis (D) genetic distances indicated little genetic differentiation among subpopulations, though some southern local subpopulations (Haenam and Sachon) were relatively different of northern subpopulations (Andong and Kunwi). Estimated number of migrants per generation was 5.9 among host subpopulations, 10.6 among geographical subpopulations, and 31.8 among seasonal subpopulations. These genetic analyses suggest that Korean S. exigua subpopulations have little genetic differentiation mostly due to their significant migratory capacity.

• The Korean Journal of Ecology
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• v.27 no.1
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• pp.35-41
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• 2004

Phragmites australis is the dominant and constructive species among plant communities in the wetlands of the downstream of Yellow River, China. Its morphological characters were high variable in different habitats. Studies on Morphological and RAPD variation of 15 P. australis populations from this region showed that soil salinity was the dominant ecological factor that affected the morphological characters of P. australis. The basal diameter, height, leaf length, leaf width, internode length, internode accounts, panicle length were negatively related to salinity. 194 loci were amplified by RAPD, of which 9 loci was highly negative-related to salinity, and showed a tendency to prefer the habitats with fresh water. 4 loci were positively related to the salinity, and showed a tendency to prefer the salinized habitats. Most loci were neutral to salinity. The morphological and genetic characters of BZH were special, and the speciality should not be determined by salinity. The morphological characters were affected by genetic information and environment. The morphological characters should change gradually and continuously along environmental gradient under plasticity, but should changed continuously or not in genetic control. The relevancies among quantitive characters, ecological factors and genetic variation in natural populations still will still be a focus and difficulty of ecological genetics of P. australis in the future.

• The KIPS Transactions:PartD
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• v.18D no.2
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• pp.89-100
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• 2011

The CNV (Copy Number Variation) which is one of the genetic structural variations in human genome is closely related with the function of gene. In particular, the genome-wide association studies for genetic diseased persons have been researched. However, there have been few studies which infer the genetic function of CNV with normal human. In this paper, we propose the analysis method to reveal the functional relationship between common CNV and genes without considering their genomic loci. To achieve that, we propose the data integration method for heterogeneity biological data and novel measurement which can calculate the correlation between common CNV and genes. To verify the significance of proposed method, we has experimented several verification tests with GO database. The result showed that the novel measurement had enough significance compared with random test and the proposed method could systematically produce the candidates of genetic function which have strong correlation with common CNV.

• Korean Journal of Plant Taxonomy
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• v.53 no.1
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• pp.14-24
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• 2023

Saussurea dorogostaiskii Palib. (Asteraceae) is a critically endangered medicinal plant in Mongolia and Russia. We studied the genetic variation of S. dorogostaiskii from three mountains of northern Mongolia. The genetic profile was assessed in 70 individuals from eight populations using five inter-simple sequence repeat markers, producing 53 loci with 96.4% polymorphism across all bands. Shannon's index (I) and Nei's gene diversity (H) value at the species level of S. dorogostaiskii are 0.25 and 0.17, respectively. An AMOVA showed high genetic variation among the populations (22% of populations and 32% of mountains), consistent with the high genetic differentiation (G_ST = 0.49) and low gene flow (N_m = 0.51) in S. dorogostaiskii populations. Eight populations were clustered into two groups, corresponding to their geographic locations. The low within-population genetic diversity and high genetic differentiation among S. dorogostaiskii populations factor into their endangered designation. This genetic analysis reveals that all populations are equally threatened, and community-based conservation is appropriate for these species.

• ALGAE
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• v.30 no.2
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• pp.89-101
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• 2015

Ecklonia cava Kjellman is a common kelp found in shallow subtidal in warm-temperate waters in the northwest Pacific Ocean. This species has shown substantial morphological variation along with subsistence in different locations and local environments. We quantified the magnitude of morphological variation of E. cava from six populations along ~700 km of coastline from Jeju Island to Dokdo in Korea. In addition, we examined genetic distance among the populations using random amplified polymorphic DNA (RAPD) analysis. Most morphological characteristics investigated were significantly different among locations. Multivariate analyses indicated two phenetically distinct groups (nearshore, sheltered vs. offshore, exposed), indicating wave exposure with turbidity are presumably major factors for the separation. With RAPD data, results of Nei's diversity (H) and AMOVA showed considerable variations in within- and between-populations. Pairwise ${\Phi}_{ST}$ and $N_m$ values indicated moderate gene flow between the six locations. Results of Nei's analysis revealed three genetically distinct groups, not consistent with the morphological groupings, indicating that a time gap may exist between morphological and genetic variations. This study also suggests dispersal distance of this kelp may be longer than what is commonly thought and genetic similarity in the populations was largely reflected by the direction of ocean current rather than just geographical distance.