Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Variation of Korean Masu Salmon (Oncorhynchus masou) Populations Inferred from Mitochondrial DNA Sequence Analysis

  • Yoon, Moon-Geun (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Jin, Hyung-Joo (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Seong, Ki-Baek (Cold Water Inland Fisheries Research and Development Institute, NFRDI) ;
  • Jin, Deuk-Hee (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • Published : 2008.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

We analyzed the nucleotide sequences of about 500 bp of the mitochondrial NADH dehydrogenase subunit 3 (ND3) gene to estimate the genetic variation of Korean masu salmon (Oncorhynchus masou) populations. DNA samples were collected from 104 river-only specimens and 52 anadromous specimens from three hatcheries and one river. There are no records of artificial release into the river. We amplified the ND3 gene by polymerase chain reaction, targeting areas that included parts of the cytochrome oxidase III gene and the NADH dehydrogenase subunit 4L gene, and defined 14 haplotypes based on 12 variable nucleotide sites in the examined region. Among the haplotypes, ten were specific to river-only specimens within hatchery populations. Haplotype diversity of river-only populations in hatcheries was higher than that of anadromous and wild populations. Pairwise population $F_{ST}$ estimates and neighbor-joining tree analyses inferred that anadromous and river-only populations were distinct. These results suggest that sequence polymorphism in the ND3 region may be a useful marker for analyzing the genetic variation and population structure of masu salmon.

Keywords

References

  1. Avise, J.C,. 1994. Molecular Markers, Natural History and Evolution. Chapman and Hall, London, UK, 478-541
  2. Brown, W.M., M. George, Jr. and A.C. Wilson. 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci., USA, 76, 1967-1971
  3. Frankel, O.H. and M.E. Soule. 1981. Conservation and Evolution. Cambridge University Press, New York, USA, 1-327
  4. Hong, K.P., J.G. Myoung, J.K. Son and C.W. Park. 1994. A biochemical study for the development of genetic marker on salmonids in Korea. Bull. Kor, Fish. Soc., 27, 83-88
  5. Kato, F. 1991. Life histories of masu and amago salmon (Oncorhynchus masou and O. rhodurus). In: Pacific Salmon Life Histories, Groot C. and L. Margolis, eds. University of British Columbia Press, Vancouver, Canada, 447-520
  6. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16, 111-120 https://doi.org/10.1007/BF01731581
  7. Lee, H.J., J.Y. Park, J.H. Lee, K.S.Min, I.G. Jeon, M.A. Yoo and W.H. Lee. 2000. Phylogeny of the subfamily salmoninae distributed in Korea based upon nucleotide sequences of mitochondrial ribosomal RNA genes. J. Kor. Fish. Soc., 33, 103-109
  8. McElroy, D., P. Moran, E. Bermingham and I. Kornfield. 1993. REAP: An integrated environment for the manipulation and phylogenetic analysis of restriction data. J. Hered., 83, 157-158
  9. McKay, S.J., R.H. Devlin and M.J. Smith. 1996. Phylogeny of Pacific salmon and trout based on growth hormone type-2 and mitochondrial NADH dehydrogenase subunit 3 DNA sequences. Can. J. Fish. Aquat. Sci., 53, 1165-1176 https://doi.org/10.1139/cjfas-53-5-1165
  10. Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York, USA
  11. Nei, M., T. Maruyama and R. Chakraborty. 1975. The bottleneck effect and genetic variability in populations. Evolution, 29, 1-10 https://doi.org/10.2307/2407137
  12. Nei, M. and F. Tajima. 1981. DNA polymorphism detectable by restriction endonucleases. Genetics, 97, 145-163
  13. Oohara, I., K. Sawano and T. Okazaki. 1997. Mito-chondrial DNA sequence analysis of the masu salmon-phylogeny in the genus Oncorhynchus. Mol. Phylogenet. Evol., 7, 71-78 https://doi.org/10.1006/mpev.1996.0373
  14. Park, L.K., M.A. Brainard, D.A. Dightman and G.A. Winans. 1993. Low levels of intraspecific variation in the mitochondrial DNA of chum salmon (Oncorhynchus keta). Mol. Mar. Biol. Biotechnol., 2, 362-370
  15. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York, USA, 9.16-9.23
  16. Sato, S., J. Ando, H. Ando, S. Urawa, A. Urano and S. Abe. 2001. Genetic variation among Japanese populations of chum salmon inferred from the nucleotide sequences of the mitochondrial DNA control region. Zool. Sci., 18, 99-106 https://doi.org/10.2108/zsj.18.99
  17. Seeb, L. W. and P.A. Crane. 1999. High genetic heterogeneity in chum salmon in western Alaska, the contact zone between northern and southern lineages. Trans. Am. Fish. Soc., 128, 58-87 https://doi.org/10.1577/1548-8659(1999)128<0058:HGHICS>2.0.CO;2
  18. Slatkin, M. and R.R. Hudson. 1991. Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations. Genetics, 129, 555-562
  19. Taniguchi, N., R. Perew-Enriquez and E. Nugroho. 2003. DNA markers as a tool for genetic management of brood stock for aquaculture. In: Aquatic Genomics Steps Toward a Great Future. Shimizu, N., T. Aoki, I. Hirono and F. Takashima, eds. Springer-Verlag, Tokyo, Japan, 417-429
  20. Verspoor, E., E.M. McCarthy and D. Knox. 1999. The phylogeography of European Atlantic salmon (Salmo salar L.) based on RFLP analysis of the NDl/ 16S RNA region of the mtDNA. Biol. J. Linn. Soc., 68, 129-146 https://doi.org/10.1111/j.1095-8312.1999.tb01162.x