• BMB Reports
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• 제57권1호
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• pp.50-59
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• 2024

The application of gene engineering in livestock is necessary for various reasons, such as increasing productivity and producing disease resistance and biomedicine models. Overall, gene engineering provides benefits to the agricultural and research aspects, and humans. In particular, productivity can be increased by producing livestock with enhanced growth and improved feed conversion efficiency. In addition, the application of the disease resistance models prevents the spread of infectious diseases, which reduces the need for treatment, such as the use of antibiotics; consequently, it promotes the overall health of the herd and reduces unexpected economic losses. The application of biomedicine could be a valuable tool for understanding specific livestock diseases and improving human welfare through the development and testing of new vaccines, research on human physiology, such as human metabolism or immune response, and research and development of xenotransplantation models. Gene engineering technology has been evolving, from random, time-consuming, and laborious methods to specific, time-saving, convenient, and stable methods. This paper reviews the overall trend of genetic engineering technologies development and their application for efficient production of genetically engineered livestock, and provides examples of technologies approved by the United States (US) Food and Drug Administration (FDA) for application in humans.

• 대한원격탐사학회지
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• 제33권5_2호
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• pp.699-708
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• 2017

본 연구에서는 재배 방법, 토양 특성 등의 정보를 상세하게 수집하기 어려운 지역단위의 콩 작황을 작물생육 모델을 이용하여 예측하는 방법을 개발하고자 하였다. 작물 생육 모델은 DSSAT에 포함된 CROPGRO-Soybean 모델을 이용하였고, 미국의 주요 콩 생산지역인 Illinois주를 연구 사례지역으로 선택하였다. CROPGRO-Soybean 모델을 이용하여 Illinois주의 콩 수량을 예측하기 위한 첫 단계로 다양한 성숙군에 속하는 국내외 품종들을 수집하여 서울대학교농장($37.27^{\circ}N$, $126.99^{\circ}E$)에서 2년동안 파종기 실험을 하여 성숙군(maturity group) I~VI까지의 성숙군별 대표 품종모수(genetic coefficients)를 추정하였다. 대표 품종모수는 각 성숙군 내에 포함되어 있는 품종들의 발육을 매우 정확하게 추정하였다. $10km{\times}10km$ 격자 단위의 기상자료를 바탕으로 성숙군(3), 파종시기(3), 관개여부(2) 등을 조합하여 18가지 조건으로 2000년에서 2011년까지 수량을 각각 모의 하였다. 성숙군과 파종시기는 Illinois주를 위도에 따라 3등분하여 각각 다르게 설정하였다. 관개 및 무관개 조건으로 구분하여 격자 별 모의결과로부터 Illinois주 전체 평균 모의수량을 구하여 연도 별 통계 수량과 비교한 결과 두 경우 모두 실제 수량과 큰 차이를 보일 뿐만 아니라 연차에 따른 수량 변동과 증가 경향을 반영하지 못하였다. 이러한 한계를 극복하고자 처리 별 격자 별로 모의된 수량을 수량을 18개 모의 조건 별로 평균하여 구한 9개 농업지구의 연도별 수량을 독립 변수, 농업지구의 연도별 통계수량을 종속 변수로 하는 중회귀 모델을 구축하였다. 18개 모의 조건 별 수량 외에 품종 개량, 재배 기술 발전 등에 따른 수량의 연차적 변화경향을 반영하기 위하여 연도를 독립변수로 추가하였으며, 중회귀모델은 농업지구와 연도별 수량 변이를 비교적 잘 예측($R^2=0.61$, n=108)하였다. 중회귀 모델로 추정한 9개 농업지구의 연도별 수량을 농업지구별 재배 면적으로 가중 평균한 Illinois의 연도별 추정수량은 통계수량에 매우 근사하였다($R^2=0.80$). 뿐만 아니라 모델 구축 대상연도가 아니고 가뭄으로 수량이 크게 감소한 2012년의 예측 수량은 $3006kg\;ha^{-1}$로 통계수량 $2890kg\;ha^{-1}$과 $116kg\;ha^{-1}$의 근사한 차이를 보였다.

• 한국자원식물학회지
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• 제21권1호
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• pp.36-40
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• 2008

본 연구는 호도나무 유경접목 활착율 개선을 통한 효율적인 접목묘 생산 및 원활한 보급체계 구축을 위하여 접목 후 이식유형 및 처리에 따른 활착율과 생장에 미치는 영향을 조사하였다. 호도나무 유경접목 후 이식유형에 따른 처리별 평균 접목활착율을 조사한 결과, 접목상 이식 후 비닐덮개 처리구(TPGB1)가 89.02%로 가장 우수하였으며 처리간 온도와 습도유지 정도에 따라 활착율의 차이가 있었다. 품종별 평균 접목활착율은 KWN-3 개체가 81.59%로 가장 높았으며 모든 처리에서도 높은 활착율을 보였다. 동일처리 내에서도 품종별 활착율의 차이가 심한 것은 접수의 영양상태와 채취부위 등이 접목활착율과 밀접한 관계가 있는 것으로 판단되었다. 접목 후 이식유형에 따른 활착묘의 생장량을 조사한 결과, 접목묘의 묘고생장 및 직경생장, 접수의 직경생장 등 조사항목 모두 처리별 활착율과 동일한 경향을 나타냈다. 묘고생장은 TPGB1이 평균 15.97cm($2.0{\sim}59.0cm$)로 가장 높은 생장량을 나타냈으며 신초지 및 접수의 직경생장에서도 모두 TPGB1 처리구가 평균 7.55mm($1.65{\sim}14.71mm$)과 8.12mm($1.82{\sim}13.58mm$)로 가장 높은 생장량을 보였다. 접목묘 신초지의 발생위치에 따른 처리별 생장량의 비교분석에서 묘고생장에서만 측아가 12.05cm로 정아의 9.57cm 보다 매우 우수하였으며 동일 처리내 접수채취 부위 및 신초지 발생위치 따른 활착율은 품종간 차이가 없었다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권1호
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• pp.69-73
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• 2012

Cancer is a complex disease and the genetic susceptibility to it could be an outcome of the inherited difference in the capacity of xenobiotic metabolizing enzymes. Glutathione-S-transferases (GSTs) are phase II metabolizing enzymes whose various genotypes have been associated with increased risk of different types of cancer. Null mutations caused by the deletion of the entire gene result in the absence of the enzymatic activity and increase in the risk of developing cancer including chronic myeloid leukaemia (CML). In the present case-control study we evaluated the effect of null mutations in GSTM1 and GSTT1 genes on the risk of developing CML. The study included 75 CML patients (43 males and 32 females; age (mean ${\pm}$ S.D) $42.3{\pm}13.4$ years) and unrelated non-malignant controls (76 male and 48 females; age (mean ${\pm}$ S.D) $41.5{\pm}12.9$). The distribution of GSTM1 and GSTT1 genotypes in CML patients and controls was assessed by multiplex-PCR method. Logistic regression was used to assess the relationship between GSTM1 and GSTT1 genotypes and risk of CML. Chi-square test was used to evaluate the trend in modulating the risk to CML by one or more potential high risk genotype. Although GSTM1 null genotype frequency was higher in CML patients (41%) than in the controls (35%), it did not reached a statistical significance (OD = 1.32, 95% CI: 0.73-2.40; P value = 0.4295). The frequency of GSTT1 null genotypes was higher in the CML patients (36%) than in the controls (21%) and the difference was found to be statistically significant (OD = 2.12, 95% CI: 1.12-4.02; P value = 0.0308). This suggests that the presence of GSTT1genotype may have protective role against the CML. We found a statistically significant (OD = 3.09, 95% CI: 1.122-8.528; P value = 0.0472) interaction between the GSTM1 and GSTT1 null genotypes and thus individuals carrying null genotypes of both GSTM1 and GSTT1 genes are at elevated risk of CML.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1511-1517
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• 2013

This study was performed to estimate the effect of age at first calving and first two calving intervals on productive life and life time profit in Korean Holsteins. Reproduction data of Korean Holsteins born from 1998 to 2004 and lactation data from 276,573 cows with birth and last dry date that calved between 2000 and 2010 were used for the analysis. Lifetime profit increased with the days of life span. Regression of Life Span on Lifetime profit indicated that there was an increase of 3,800 Won (approximately $3.45) of lifetime profit per day increase in life span. This is evidence that care of each cow is necessary to improve net return and important for farms maintaining profitable cows. The estimates of heritability of age at first calving, first two calving intervals, days in milk for lifetime, lifespan, milk income and lifetime profit were 0.111, 0.088, 0.142, 0.140, 0.143, 0.123, and 0.102, respectively. The low heritabilities indicated that the productive life and economical traits include reproductive and productive characteristics. Age at first calving and interval between first and second calving had negative genetic correlation with lifetime profit (-0.080 and -0.265, respectively). Reducing age at first calving and first calving interval had a positive effect on lifetime profit. Lifetime profit increased to approximately 2,600,000 (2,363.6) from 800,000 Won ($727.3) when age at first calving decreased to (22.3 month) from (32.8 month). Results suggested that reproductive traits such as age at first calving and calving interval might affect various economical traits and consequently influenced productive life and profitability of cows. In conclusion, regard of the age at first calving must be taken with the optimum age at first calving for maximum lifetime profit being 22.5 to 23.5 months. Moreover, considering the negative genetic correlation of first calving interval with lifetime profit, it should be reduced against the present trend of increase.

• Asian Pacific Journal of Cancer Prevention
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• 제15권4호
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• pp.1603-1609
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• 2014

Background: The response to treatment and overall survival (OS) of patients with acute myeloid leukemia (AML) is variable, with a median ranging from 6 months to 11.5 years. TP53 is associated with old age, chemotherapy resistance, and worse OS. Using genetic sequencing, we set out to look at our own experience with AML, and hypothesized that both TP53 mutations and SNPs at codon 72 would mimic the literature by occurring in a minority of patients, and conferring a worse OS. Materials and Methods: We performed a pilot study of randomly selected, newly diagnosed AML patients at Mount Sinai Medical Center, diagnosed from 2005-2008 (n=10). TP53 PCR sequencing was performed using DNA from bone marrow smears. Analysis was accomplished using Mutation Surveyor software with confirmation of the variants using the COSMIC and dbSNP databases. Results: Fewer than half of the patients harbored TP53 mutations (40%). There was no significant difference in OS based on gender, AML history, risk-stratified karyotype, or TP53 mutation. There were possible trends toward improved survival among patients less than 60 (11 vs 4 months, p=0.09), Hispanics (8 vs 1 months, p=0.11), and those not harboring SNP P72R (8 vs 2 months, p=0.10). There was a significant improvement in survival among patients with better performance status (28 vs 4 months, p=0.01) and those who did not have a complex karyotype (8 vs 1 months, p=0.03). The most commonly observed TP53 mutation was a missense N310K (40%) and the most commonly observed SNP was P72R (100.0%). Conclusions: Our study confirms previous reports that poor PS and the presence of a complex karyotype are associated with a decreased OS. In our cohort, TP53 mutations were relatively common, occurring more frequently in male patients with an adverse karyotype. Although there was no significant difference in survival between TP53 mutated and un-mutated patients, there was a possible trend toward worse OS among patients with SNP P72R. Larger studies are needed to validate these findings.

• Journal of Animal Science and Technology
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• 제55권3호
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• pp.173-178
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• 2013

본 연구는 홀스타인 젖소, 436,690두의 검정일 유량기록 총 4,366,900개를 이용하여 비유지속성에 대한 특성과 새로운 평가형질로서의 가능성을 조사하는데 있다. 1산차, 2산차와 3산차 이상의 평균 비유지속성은 각각 97.5%, 95.1%와 94.6%였으며, 최고 유량 도달 이후 비유지속성이 일괄적으로 감소하는 추세를 나타내었다. 분만 후 최고유량에 도달하는 평균 비유일수는 약 50일로 조사 되었으나 실제로 2차 검정(36~66일)시에 최고 유량에 도달하는 개체의 기록은 전체 자료의 33.2%에 불과하였다. 또한 1차 검정시에 최고 유량에 도달한 개체들의 비유일수에 따른 유생산량 변화는 2~4차 검정에서 최고유량을 나타내는 개체들의 변화와는 많은 차이를 나타내었다. 평균 비유지속성에 대한 유전력과 반복력은 각각 0.16과 0.35로 추정되었으며, 10차 검정시 평균 비유지속성에 대한 검정차별 유전상관을 보면 7차 검정시 이상일 때 0.91이상이었으며, 검정차수가 줄어들수록 유전상관은 급격히 감소하였다. Data I에 대한 Data II와 III의 육종가 상관은 각각 0.80과 0.72, 순위 상관은 각각 0.78과 0.71로 나타났다. 결과적으로 자료가 추가될수록 육종가 및 순위 상관은 낮게 나타났다.

• Clinical and Experimental Pediatrics
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• 제55권10호
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• pp.388-392
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• 2012

Purpose: Single-nucleotide polymorphism (SNP) markers within LIN28B have been reported to be related to the timing of pubertal growth. However, no study has investigated the frequency of genetic markers in girls with precocious puberty (PP) or early puberty (EP). This study aimed to determine the frequency of putative genetic markers in girls with PP or EP. Methods: Genomic DNAs were obtained from 77 and 109 girls that fulfilled the criteria for PP and EP, respectively. The controls in this study were 144 healthy volunteers between 20 and 30 years of age. The haplotypes were reconstructed using 11 SNPs of LIN28B, and haplotype association analysis was performed. The haplotype frequencies were compared. Differences in the clinical and laboratory parameters were analyzed according to the haplotype dosage. Results: Eleven SNPs in LIN28B were all located in a block that was in linkage disequilibrium. The haplotype could be reconstructed using 2 representative SNPs, rs4946651 and rs369065. The AC haplotype was less frequently observed in the PP group than in the controls (0.069 vs. 0.144, P=0.010). The trend that girls with non-AC haplotypes tended to have earlier puberty onset (P=0.037) was illustrated even in the EP+PP patient group by Kaplan-Meier analysis. Conclusion: The results of the present study showed that non-AC haplotypes of LIN28B had a significant association with PP in girls.

• Radiation Oncology Journal
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• 제36권3호
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• pp.218-226
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• 2018

Purpose: Standard treatment for locally advanced rectal cancer consists of neoadjuvant radiochemotherapy with concomitant fluoropyrimidine or oxaliplatin and surgery with curative intent. Pathological complete response has shown to be predictive for better outcome and survival; nevertheless there are no biological or genetic factors predictive for response to treatment. We explored the correlation between the single nucleotide polymorphisms (SNPs) GSTP1 (A313G) and XRCC1 (G28152A), and the pathological complete response and survival after neoadjuvant radiochemotherapy in locally advanced rectal cancer patients. Materials and Methods: Genotypes GSTP1 (A313G) and XRCC1 (G28152A) were determined by pyrosequencing technology in 80 patients affected by locally advanced rectal cancer. Results: The overall rate of pathological complete response in our study population was 18.75%. Patients homozygous AA for GSTP1 (A313G) presented a rate of pathological complete response of 26.6% as compared to 8.5% of the AG+GG population (p = 0.04). The heterozygous comparison (AA vs. AG) showed a significant difference in the rate of pathological complete response (26.6% vs. 6.8%; p = 0.034). GSTP1 AA+AG patients presented a 5- and 8-year cancer-specific survival longer than GSTP1 GG patients (87.7% and 83.3% vs. 44.4% and 44.4%, respectively) (p = 0.014). Overall survival showed only a trend toward significance in favor of the haplotypes GSTP1 AA+AG. No significant correlations were found for XRCC1 (G28152A). Conclusion: Our results suggest that GSTP1 (A313G) may predict a higher rate of pathological complete response after neoadjuvant radiochemotherapy and a better outcome, and should be considered in a more extensive analysis with the aim of personalization of radiation treatment.

• 자원환경지질
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• 제19권spc호
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• pp.53-71
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• 1986

옥방중석광상(玉房重石鑛床)에서의 광화작용(鑛化作用)의 특성(特性)을 규명(糾明)하기 위(爲)하여 광역적(廣域的)인 지질조사(地質調査)와 여러 암층(岩層)들에 대(對)한 주성분원소(主成分元素), 미량원소(微量元素)의 지화학적(地化學的) 경향(傾向)을 검토(檢討)하였으며, 상동지역(上東地域)과 상호(相互) 비교(比較)를 해 보았다. 이 광상(鑛床)에서의 중석광화작용(重石鑛化作用)은 각섬석내(角閃石內)에 발달(發達)된 페그마타이트에서만 있었고 편암(片岩)이나 편마암(片麻岩) 부근(附近)에 발달(發達)된 페그마타이트에는 광화작용(鑛化作用)의 흔적(痕迹)이 보이지 않는다. 중석(重石)의 농집(濃集)은 지화학적(地化學的)으로 $K_2O$, $Na_2O$, Rb이 증가(增加)하고 Sr이 감소(減少)할 때 나타나며, Rb/Sr 비(比)의 경향(傾向)로 볼 때 페그마타이트내(內) 중석(重石)의 유입(流入)은 Rb 농집(濃集)과 Sr 감소(減少)를 수반(隨伴)한 열수작용(熱水作用)에 기인(起因)되었음을 알 수 있다. 이러한 양상(樣相)은 화강암(花崗巖) 혹은 페그마타이트를 모암(母岩)으로 하는 광상(鑛床)들에 대(對)하여 탐광(探鑛)의 지침(指針)이 될 수 있다. 이 지역(地域)에 있는 화강암(花崗巖)이 회중석(灰重石)의 성인(成因)에 대(對)하여 공간적(空間的)으로나 시간적(時間的)으로 영향(影響)을 미친 증거(證據)는 관찰(觀察)되지 않았다.