• 한국공간구조학회논문집
• /
• 제14권4호
• /
• pp.97-104
• /
• 2014

Recently, a concept of damped outrigger system has been proposed for tall buildings. Structural characteristics and design method of this system were not sufficiently investigated to date. In this study, control performance of damped outrigger system for building structures subjected to seismic excitations has been investigated. And optimal design method of damped outrigger system has been proposed using multi-objective genetic algorithm. To this end, a simplified numerical model of damped outrigger system has been developed. State-space equation formulation proposed in previous research was used to make a numerical model. Multi-objective genetic algorithms has been employed for optimal design of the stiffness and damping parameters of the outrigger damper. Based on numerical analyses, it has been shown that the damped outrigger system control dynamic responses of the tall buildings subjected to earthquake excitations in comparison with a traditional outrigger system.

• Journal of information and communication convergence engineering
• /
• 제6권4호
• /
• pp.448-453
• /
• 2008

This study describes design and development techniques of estimation models for process modeling. One case study is undertaken to design a model using standard gas furnace data. Neural networks (NN) and genetic programming (GP) are each employed to model the crucial relationships between input factors and output responses. In the case study, two models were generated by using 70% training data and evaluated by using 30% testing data for genetic programming and neural network modeling. The model performance was compared by using RMSE values, which were calculated based on the model outputs. The average RMSE for training and testing were 0.8925 (training) and 0.9951 (testing) for the NN model, and 0.707227 (training) and 0.673150 (testing) for the GP model, respectively. As concern the results, the NN model has a strong advantage in model training (using the all data for training), and the GP model appears to have an advantage in model testing (using the separated data for training and testing). The performance reproducibility of the GP model is good, so this approach appears suitable for modeling physical fabrication processes.

• Journal of Genetic Medicine
• /
• 제13권1호
• /
• pp.1-13
• /
• 2016

Although some mutations are beneficial and are the driving force behind evolution, it is important to maintain DNA integrity and stability because it contains genetic information. However, in the oxygen-rich environment we live in, the DNA molecule is under constant threat from endogenous or exogenous insults. DNA damage could trigger the DNA damage response (DDR), which involves DNA repair, the regulation of cell cycle checkpoints, and the induction of programmed cell death or senescence. Dysregulation of these physiological responses to DNA damage causes developmental defects, neurological defects, premature aging, infertility, immune system defects, and tumors in humans. Some human syndromes are characterized by unique neurological phenotypes including microcephaly, mental retardation, ataxia, neurodegeneration, and neuropathy, suggesting a direct link between genomic instability resulting from defective DDR and neuropathology. In this review, rare human genetic disorders related to abnormal DDR and damage repair with neural defects will be discussed.

• Mycobiology
• /
• 제41권3호
• /
• pp.121-125
• /
• 2013

Arbuscular mycorrhizal fungi (AMF) have mutualistic relationships with more than 80% of terrestrial plant species. This symbiotic relationship is ancient and would have had important roles in establishment of plants on land. Despite their abundance and wide range of relationship with plant species, AMF have shown low species diversity. However, molecular studies have suggested that diversity of these fungi may be much higher, and genetic variation of AMF is very high within a species and even within a single spore. Despite low diversity and lack of host specificity, various functions have been associated with plant growth responses to arbuscular mycorrhizal fungal colonization. In addition, different community composition of AMF affects plants differently, and plays a potential role in ecosystem variability and productivity. AMF have high functional diversity because different combinations of host plants and AMF have different effects on the various aspects of symbiosis. Consequently, recent studies have focused on the different functions of AMF according to their genetic resource and their roles in ecosystem functioning. This review summarizes taxonomic, genetic, and functional diversities of AMF and their roles in natural ecosystems.

• Toxicological Research
• /
• 제35권4호
• /
• pp.319-330
• /
• 2019

Adverse drug reactions (ADRs) constitute key factors in determining successful medication therapy in clinical situations. Integrative analysis of electronic medical record (EMR) data and use of proper analytical tools are requisite to conduct retrospective surveillance of clinical decisions on medications. Thus, we suggest that electronic medical recording and human genetic databases are considered together in future directions of pharmacovigilance. We analyzed EMR-based ADR studies indexed on PubMed during the period from 2005 to 2017 and retrospectively acquired 1161 (29.6%) articles describing drug-induced adverse reactions (e.g., liver, kidney, nervous system, immune system, and inflammatory responses). Of them, only 102 (8.79%) articles contained useful information to detect or predict ADRs in the context of clinical medication alerts. Since insufficiency of EMR datasets and their improper analyses may provide false warnings on clinical decision, efforts should be made to overcome possible problems on data-mining, analysis, statistics, and standardization. Thus, we address the characteristics and limitations on retrospective EMR database studies in hospital settings. Since gene expression and genetic variations among individuals impact ADRs, pharmacokinetics, and pharmacodynamics, appropriate paths for pharmacovigilance may be optimized using suitable databases available in public domain (e.g., genome-wide association studies (GWAS), non-coding RNAs, microRNAs, proteomics, and genetic variations), novel targets, and biomarkers. These efforts with new validated biomarker analyses would be of help to repurpose clinical and translational research infrastructure and ultimately future personalized therapy considering ADRs.

• Journal of Ginseng Research
• /
• 제35권1호
• /
• pp.86-91
• /
• 2011

Ginsenoside (G)-F1 is an enzymatic metabolite generated from G-Rg1. Although this metabolite has been reported to suppress platelet aggregation and to reduce gap junction-mediated intercellular communication, the modulatory activity of G-F1 on the functional role of skin-derived cells has not yet been elucidated. In this study, we evaluated the regulatory role of G-F1 on the cellular responses of B16 melanoma cells. G-F1 strongly suppressed the proliferation of B16 cells up to 60% at 200 ${\mu}g/mL$, while only diminishing the viability of HEK293 cells up to 30%. Furthermore, G-F1 remarkably induced morphological change and clustering of B16 melanoma cells. The melanin production of B16 cells was also significantly blocked by G-F1 up to 70%. Interestingly, intracellular signaling events involved in cell proliferation, migration, and morphological change were up-regulated at 1 h incubation but down-regulated at 12 h. Therefore, our results suggest that G-F1 can be applied as a novel anti-skin cancer drug with anti-proliferative and anti-migration features.

• 한국공간구조학회논문집
• /
• 제10권4호
• /
• pp.93-102
• /
• 2010

본 연구에서는 MR 감쇠기와 FPS를 사용하여 구성된 스마트 스카이브릿지를 제안하였으며 스마트 스카이브릿지로 연결된 인접건물의 지진응답 제어성능을 분석하였다. 이를 위하여 스마트 스카이브릿지로 연결된 10층과 20층 구조물을 예제 구조물로 선택하였고 근거리 (near fault) 및 원거리 (far fault) 지진의 특성을 가지는 El Centro 지진과 Kobe지진을 사용하여 시간이력해석을 수행하였다. 스마트 스카이브릿지블 효과적으로 제어하기 위해서 퍼지논리제어기를 개발하였으며 퍼지논리제어기를 최적화하기 위하여 다목적 유전자알고리즘을 사용하였다. 최적화결과 10층 건물의 지진응답과 20층 건물의 지진응답 사이에는 상충관계 (trade-off)가 있는 것을 알 수 있었고 다목적 유전자알고리즘을 통해서 두 건물의 지진응답 제어에 대한 퍼지논리제어거의 파레토 해집합을 구할 수 있었다. 수치해석결과 본 연구에서 제안한 스마트 스카이브릿지를 사용하면 연결된 건물의 지진응답을 효율적으로 저감시킬 수 있는 것을 알 수 있었다.

• Journal of Veterinary Science
• /
• 제22권3호
• /
• pp.30.1-30.15
• /
• 2021

Background: New-generation adjuvants for foot-and-mouth disease virus (FMDV) vaccines can improve the efficacy of existing vaccines. Chinese medicinal herb polysaccharide possesses better promoting effects. Objectives: In this study, the aqueous extract from Artemisia rupestris L. (AEAR), an immunoregulatory crude polysaccharide, was utilized as the adjuvant of inactivated FMDV vaccine to explore their immune regulation roles. Methods: The mice in each group were subcutaneously injected with different vaccine formulations containing inactivated FMDV antigen adjuvanted with three doses (low, medium, and high) of AEAR or AEAR with ISA-206 adjuvant for 2 times respectively in 1 and 14 days. The variations of antibody level, lymphocyte count, and cytokine secretion in 14 to 42 days after first vaccination were monitored. Then cytotoxic T lymphocyte (CTL) response and antibody duration were measured after the second vaccination. Results: AEAR significantly induced FMDV-specific antibody titers and lymphocyte activation. AEAR at a medium dose stimulated Th1/Th2-type response through interleukin-4 and interferon-γ secreted by CD4⁺ T cells. Effective T lymphocyte counts were significantly elevated by AEAR. Importantly, the efficient CTL response was remarkably provoked by AEAR. Furthermore, AEAR at a low dose and ISA-206 adjuvant also synergistically promoted immune responses more significantly in immunized mice than those injected with only ISA-206 adjuvant and the stable antibody duration without body weight loss was 6 months. Conclusions: These findings suggested that AEAR had potential utility as a polysaccharide adjuvant for FMDV vaccines.

• Journal of Microbiology and Biotechnology
• /
• 제29권8호
• /
• pp.1316-1323
• /
• 2019

Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerging coronavirus which is zoonotic from bats and camels. Its infection in humans can be fatal especially in patients with preexisting conditions due to smoking and chronic obstructive pulmonary disease (COPD). Among the 25 proteins encoded by MERS-CoV, 5 accessory proteins seem to be involved in viral evasion of the host immune responses. Here we report that ORF4a, ORF4b, and ORF8b proteins, alone or in combination, effectively antagonize nuclear factor kappa B ($NF-{\kappa}B$) activation. Interestingly, the inhibition of $NF-{\kappa}B$ by MERS-CoV accessory proteins was mostly at the level of pattern recognition receptors: melanoma differentiation-associated gene 5 (MDA5). ORF4a and ORF4b additively inhibit MDA5-mediated activation of $NF-{\kappa}B$ while that of retinoic acid-inducible gene 1 (RIG-I) is largely not perturbed. Of note, ORF8b was found to be a novel antagonist of MDA5-mediated $NF-{\kappa}B$ activation. In addition, ORF8b also strongly inhibits Tank-binding kinase 1 (TBK1)-mediated induction of $NF-{\kappa}B$ signaling. Taken together, MERS-CoV accessory proteins are involved in viral escape of $NF-{\kappa}B$-mediated antiviral immune responses.

• Journal of Microbiology and Biotechnology
• /
• 제30권12호
• /
• pp.1801-1809
• /
• 2020

Chikungunya virus (CHIKV) was first identified in 1952 as a causative agent of outbreaks. CHIKV is transmitted by two mosquito species, Aedes aegypti and A. albopictus. Symptoms after CHIKV infection in human are typically fever and joint pain, but can also include headache, muscle pain, joint swelling, polyarthralgia, and rash. CHIKV is an enveloped single-stranded, positive-sense RNA virus with a diameter of approximately 70 nm. The pathogenesis of CHIKV infection and the mechanism by which the virus evades the innate immune system remain poorly understood. Moreover, little is known about the roles of CHIKV-encoded genes in the viral evasion of host immune responses, especially type I interferon (IFN) responses. Therefore, in the present study, we screened CHIKV-encoded genes for their regulatory effect on the activation of nuclear factor kappa B (NF-κB), a critical transcription factor for the optimal activation of IFN-β. Among others, non-structural protein 2 (nsP2) strongly inhibited melanoma differentiation-associated protein 5 (MDA5)-mediated induction of the NF-κB pathway in a dose-dependent manner. Elucidation of the detailed mechanisms of nsP2-mediated inhibition of the MDA5/RIG-I signaling pathway is anticipated to contribute to the development of virus-specific therapeutics against CHIKV infection.