• Asian-Australasian Journal of Animal Sciences
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• 제15권7호
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• pp.944-948
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• 2002

The aim of this study was to estimate heritability and repeatability for the number of embryos and transferable embryos collected per flush in Holstein population in Hokkaido, Japan. Data consisted of 306 MOET (Multiple Ovulation and Embryo Transfer) treatments on 224 Holstein cows from 1997 to 2000. Variance components for these traits were estimated using the REML procedure. The model included only non-genetic factors that were significant at the 0.05 level, through using generalized linear models, maximum likelihood methods, and stepwise regression procedure as fixed effects and sire and residual for heritabilities, donor and residual for repeatabilities as random effects. The factor identified as important in determining the results was the donor''s estrous condition after superovulation. Heritabilities for the number of embryos and transferable embryos collected per flush were 0.14 and 0.09, respectively. The corresponding repeatabilities were 0.43 and 0.32, respectively. These results show that it was difficult to genetically improve these traits, thus, environmental and physical factors affecting the donor must be improved. These results also show that it is necessary to take the donor''s estrous condition after superovulation and repeatabilities for the number of embryos and transferable embryos collected per flush into account when the genetic gains and inbreeding rates for MOET breeding schemes are predicted by a computer simulation.

• Journal of Mechanical Science and Technology
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• 제20권8호
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• pp.1125-1138
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• 2006

The optimal design of the squeeze film damper (SFD) for rotor system has been studied in previous researches. However, these researches have not been considering jumping or nonlinear phenomena of a rotor system with SFD. This paper represents an optimization technique for linear and nonlinear response of a simple rotor system with SFDs by using a hybrid GA-SA algorithm which combined enhanced genetic algorithm (GA) with simulated annealing algorithm (SA). The damper design parameters are the radius, length and radial clearance of the damper. The objective function is to minimize the transmitted load between SFD and foundation at the operating and critical speeds of the rotor system with SFD which has linear and nonlinear unbalance responses. The numerical results show that the transmitted load of the SFD is greatly reduced in linear and nonlinear responses for the rotor system.

• 한국공간구조학회논문집
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• 제15권2호
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• pp.105-112
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• 2015

A shared tuned mass damper (STMD) was proposed in previous research for reduction of dynamic responses of the adjacent buildings subjected to earthquake loads. A single STMD can provide similar control performance in comparison with two traditional TMDs. In previous research, a passive damper was used to connect the STMD with adjacent buildings. In this study, a smart magnetorheological (MR) damper was used instead of a passive damper to compose an adaptive smart STMD (ASTMD). Control performance of the ASTMD was investigated by numerical analyses. For this purpose, two 8-story buildings were used as example structures. Multi-input multi-output (MIMO) fuzzy logic controller (FLC) was used to control the command voltages sent to two MR dampers. The MIMO FLC was optimized by a multi-objective genetic algorithm. Numerical analyses showed that the ASTMD can effectively control dynamic responses of adjacent buildings subjected to earthquake excitations in comparison with a passive STMD.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권6호
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• pp.2056-2069
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• 2014

In order to implement interactive and personalized Web services properly, it is necessary to understand the tangible and intangible responses of the users and to recognize their emotional states. Recently, some studies have attempted to build emotional state estimation models based on facial expressions. Most of these studies have applied multiple regression analysis (MRA), artificial neural network (ANN), and support vector regression (SVR) as the prediction algorithm, but the prediction accuracies have been relatively low. In order to improve the prediction performance of the emotion prediction model, we propose a novel SVR model that is optimized using a genetic algorithm (GA). Our proposed algorithm-GASVR-is designed to optimize the kernel parameters and the feature subsets of SVRs in order to predict the levels of two aspects-valence and arousal-of the emotions of the users. In order to validate the usefulness of GASVR, we collected a real-world data set of facial responses and emotional states via a survey. We applied GASVR and other algorithms including MRA, ANN, and conventional SVR to the data set. Finally, we found that GASVR outperformed all of the comparative algorithms in the prediction of the valence and arousal levels.

• 한국동물학회지
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• 제20권4호
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• pp.159-168
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• 1977

계대 배양중인 생쥐의 임파종양 L5178Y 세포의 유전자돌연변이 유발성 검출법(Methotrexate-저항성)과 순수분리한 사람의 임파구에서의 DNA 회복복제법을 사용하여 Salmomella/microsome 시스템에서 돌연변이 유발성이 확인된 살충데 DDVP와 trichlorfon, 살균제 TMTD 및 제초제 MO와 NIP등 5종의 농약이 포유동물 세포에 미치는 유전적 영향을 조사했다. 조사한 농약중 TMTD는 상기조사한 시스템 모두에서 양성결과를 보여준 반면에 DDVP와 trichlofon은 L5178Y 세포에서의 돌연변이 유발성은 나타내지 않았으나 DNA회복 복제법에서는 양성결과를 보여주었다. MO와 NIP는 조사한 시스템 모두에서 양성결과를 나타내지않았다.

• Structural Engineering and Mechanics
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• 제39권1호
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• pp.147-168
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• 2011

To ensure safety and long term performance, structural control has rapidly matured over the past decade into a viable means of limiting structural responses to strong winds and earthquakes. Nonlinear response history analysis requires rigorous procedure to compute seismic demands. Therefore the simplified nonlinear analysis procedures are useful to determine performance of the structure. In this investigation, application of improved capacity demand diagram method in the control of structural system is presented for the first time. Developed pole assignment method (DPAM) in structural systems control is introduced. Genetic algorithm (GA) is employed as an optimization tool for minimizing a target function that defines values of coefficient matrices providing the placement of actuators and optimal control forces. The ground acceleration is modified under induced control forces. Due to this, performance of structure based on improved nonlinear demand diagram is selected to threshold of nonlinear behavior of structure. With small energy consumption characteristics, semi-active devices are especially attractive solutions for limiting earthquake effects. To illustrate the efficiency of DPAM, a 30-story steel moment frame structure employing the semi-active control devices is applied. In comparison to the widely used linear quadratic regulation (LQR), the DPAM controller was shown to be just as effective and better in the reduction of structural responses during large earthquakes.

• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1137-1143
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• 2019

Hepatitis E virus (HEV) accounts for 20 million infections in humans worldwide. In most cases, the infections are self-limiting while HEV genotype 1 infection cases may lead to lethal infections in pregnant women (~ 20% fatality). The lack of small animal models has hampered detailed analysis of virus-host interactions and HEV-induced pathology. Here, by employing a recently developed culture-adapted HEV, we demonstrated that methyltransferase, a non-structural protein, strongly inhibits melanoma differentiation-associated gene 5 (MDA5)-mediated activation of type I interferon responses. Compared to uninfected controls, HEV-infected cells display significantly lower levels of $IFN-{\beta}$ promoter activation when assessed by luciferase assay and RT-PCR. HEV genome-wide screening showed that HEV-encoded methyltransferase (MeT) strongly inhibits MDA5-mediated transcriptional activation of $IFN-{\beta}$ and $NF-{\kappa}B$ in a dose-responsive manner whether or not it is expressed in the presence/absence of a tag fused to it. Taken together, current studies clearly demonstrated that HEV MeT is a novel antagonist of MDA5-mediated induction of $IFN-{\beta}$ signaling.

• Asian-Australasian Journal of Animal Sciences
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• 제30권12호
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• pp.1674-1678
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• 2017

Objective: The aim of the study was to evaluate genetic correlations between the behavioural profile and performance in laying hens as an indirect answer to the question whether the observed behavioural responses are associated with increased levels of stress in these birds. Methods: The assessment of birds' temperament was carried out using the novel objects test. The behavioural test was conducted in two successive generations comprising 9,483 Rhode Island White (RIW) birds (approx. 4,700 individuals per generation) and 4,326 Rhode Island Red (RIR) birds (approx. 2,100 individuals per generation). Based on the recorded responses, the birds were divided into two groups: a fearful profile (1,418 RIW hens and 580 RIR hens) and a brave/curious profile (8,065 RIW hens and 3,746 RIR hens). The birds were subjected to standard assessment of their performance traits, including SM, age at sexual maturity; ST, shell thickness; SG, egg specific gravity; EW, mean egg weight; IP, initial egg production; and HC, number of hatched chicks. The pedigree was three generations deep (including two behaviourrecorded generations). Estimation of the (co)variance components was performed with the Gibbs sampling method, which accounts for the discrete character of the behavioural profile denotation. Results: The analyses revealed negative correlations between the performance traits of the laying hens and the behavioural profile defined as fearful. In the group of fearful RIW birds, delayed sexual maturation (0.22) as well as a decrease in the initial egg production (-0.30), egg weight (-0.54), egg specific gravity (-0.331), shell thickness (-0.11), and the number of hatched chicks (-0.24) could be expected. These correlations were less pronounced in the RIR breed, in which the fearful birds exhibited a decline in hatchability (-0.37), egg specific gravity (-0.11), and the number of hatched chicks (-0.18). There were no correlations in the case of the other traits or they were positive but exhibited a substantial standard error, as for the egg weight. Conclusion: To sum up the results obtained, it can be noted that behavioural responses indicating fearfulness, i.e. escape, avoidance, and approach-avoidance may reflect negative emotions experienced by birds. The negative correlations with performance in the group of fearful hens may indirectly indicate a high level of stress in these birds, especially in the white-feathered birds, where stronger performance-fearfulness correlations were found. Fearful birds should be eliminated from breeding by inclusion of the behavioural profile in the selection criterion in the case of laying hens.

• IMMUNE NETWORK
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• 제10권6호
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• pp.198-205
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• 2010

Background: A crucial limitation of DNA vaccines is its weak immunogenicity, especially in terms of eliciting antibody responses in non-human primates or humans; therefore, it is essential to enhance immune responses to vaccination for the development of successful DNA vaccines for humans. Methods: Here, we approached this issue by evaluating interleukin-7 (IL-7) as a genetic adjuvant in cynomolgus monkeys immunized with multigenic HCV DNA vaccine. Results: Codelivery of human IL-7 (hIL-7)-encoding DNA appeared to increase DNA vaccine-induced antibody responses specific for HCV E2 protein, which plays a critical role in protecting from HCV infection. HCV-specific T cell responses were also significantly enhanced by codelivery of hIL-7 DNA. Interestingly, the augmentation of T cell responses by codelivery of hIL-7 DNA was shown to be due to the enhancement of both the breadth and magnitude of immune responses against dominant and subdominant epitopes. Conclusion: Taken together, these findings suggest that the hIL-7-expressing plasmid serves as a promising vaccine adjuvant capable of eliciting enhanced vaccine-induced antibody and broad T cell responses.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2005년도 춘계 학술발표회 논문집
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• pp.209-216
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• 2005

The controller that can control the smart base isolation system consisting of M damper and friction pendulum systems(FPS) is developed in this study. A fuzzy logic controller (FLC) is used to modulate the M damper force because the FLC has an inherent robustness and ability to handle non-linearities and uncertainties. A genetic algorithm (GA) is used for optimization of the FLC. When earthquake excitations are applied to the structures equipped with smart base isolation system, the relative displacement at the isolation level as well as the acceleration of the structure should be regulated under appropriate level. Thus, NSGA-II(Non-dominated Sorting Genetic Algorithm) is employed in this study as a multi-objective genetic algorithm to meet more than two control objectives, simultaneously. NSGA-II is used to determine appropriate fuzzy control rules as well to adjust parameters of the membership functions. Effectiveness of the proposed method for optimal design of the FLC is judged based on computed responses to several historical earthquakes. It has been shown that the proposed method can efficiently find Pareto optimal sets that can reduce both structural acceleration and base drift from numerical studies.