• Plant Breeding and Biotechnology
• /
• 제7권3호
• /
• pp.186-199
• /
• 2019

We selected 68 Chinese maize inbred lines to understand the genetic diversity, population structure, and marker-trait associations for eight agronomic traits and 50 simple sequence repeats (SSRs) markers. In this study, effective traits, such as days of anthesis (DA), days of silking (DS), ear height (EH), plant to ear height ratio (ER), plant height (PH), and leaf width (LW) were divided into PC1 and PC2 by PCA analysis for maize inbred lines. Genetic diversity analysis revealed a total of 506 alleles at 50 SSR loci. The mean number of alleles per locus was 10.12. The averages of genetic diversity (GD) and polymorphic information content (PIC) values were 0.771 and 0.743, respectively. Based on a membership probability threshold of 0.80, the population structure revealed that the total inbred lines were divided into three major groups with one admixed group. A marker-trait association using Q + K MLM showed that nine SSR markers (bnlg1017, umc2041, umc2400, bnlg105, umc1229, umc1250, umc1066, umc2092, and umc1426) were related with seven agronomic traits. Among these SSR markers, eight SSR markers were associated with only one agronomic trait (DA, DS, ER, LL, LW, PH, and ST), whereas one SSR marker (umc1229) was associated with two agronomic traits (DA and ST). These results will help in optimizing the choice of inbred lines for cross combinations, as well as in selecting markers for further maize breeding programs.

• Molecules and Cells
• /
• 제24권1호
• /
• pp.60-68
• /
• 2007

Microsatellites, also called simple sequence repeats (SSR), are very useful molecular genetic markers commonly used in crop breeding, species identification and linkage analysis. In the present study, we constructed a microsatellite-enriched genomic library of Panax ginseng, and identified 251 novel microsatellite sequences. Tri-nt repeat units were the most abundant (46.6%), followed by di-nt repeats (35.5%). The $(AG)_n$ motif was most common (23.1%), followed by the $(AAC)_n$ motif (22.3%). From the genotyping of 94 microsatellites using marker-specific primer sets, we identified 11 intraspecific polymorphic markers as well as 14 possible interspecific polymorphic markers differing between P. ginseng and P. quinquefolius. The exact allele structures of the polymorphic markers were determined and the alleles were named. This study represents the first report of the bulk isolation of microsatellites by screening a microsatellite-enriched genomic library in P. ginseng. The microsatellite markers could be useful for linkage analysis, genetic breeding and authentication of Panax species.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제15권2호
• /
• pp.145-155
• /
• 2007

Mulberry (Morus spp.) is an economically important tree crop being cultivated in India, China and other sericulturally important countries for its foliage to feed the silk producing insect Bombyx mori L. Genetic improvements of mulberry lag behind to the same in many other economically less important crops due to the complexity of its genetics, the breeding behavior, and the lack of basic information on factors governing important agronomic traits. In this review, the general usage and advantages of different molecular markers including isoenzymes, RFLPs, RAPDs, ISSRs, SSRs, AFLPs and SNPs are described to enlighten their applicability in mulberry genetic improvement programs. Application of DNA markers in germplasm characterization, construction of genetic linkage maps, QTL identification and in marker-assisted selection was also described along with its present status and future prospects.

• Mycobiology
• /
• 제29권1호
• /
• pp.43-47
• /
• 2001

Genetic diversity of 21 Korean Phytophthora capsici isolates was analyzed by using several molecular markers such as random amplified polymorphic DNA(RAPD), M-13, microsatellite and random amplified microsatellite sequences(RAMS). The overall average similarity coefficient among the isolates was 86% based on the combined data obtained by the molecular markers. No molecular markers were found to be associated with hosts or geographic regions. In addition to RAPD, analysis based on repeated sequences such as $(GTG)_5$, M-13 and RAMS could be used to assess population structure of P. capsici.

• 한국산림과학회지
• /
• 제96권2호
• /
• pp.125-130
• /
• 2007

The principal morphological characters used for identification of hydrangea cultivars are often dependent on agroclimatic conditions. Furthermore, information on the selection or the genetic background of the hydrangea breeding is so rare that a molecular marker system for cultivar identification is needed. Amplified fragment length polymorphism (AFLP) markers were employed for fingerprinting Hydrangea macrophylla cultivars and candidate cultivars of H. macrophylla selected in Korea. One AFLP primer combination was sufficient to distinguish 17 H. macrophylla cultivars and 4 candidate cultivars. The profile of 19 loci that can minimize the error of amplification peak detection was constructed. AFLP markers were efficient for identification, estimation of genetic distances between cultivars, and cultivar discrimination. Based on the observed AFLP markers, genetic relationship was reconstructed by the UPGMA method. Seventeen H. macrophylla cultivars and H. macrophylla for. normalis formed a major cluster, and candidate cultivars selected in Korea formed another cluster.

• 생명과학회지
• /
• 제27권4호
• /
• pp.464-470
• /
• 2017

성염색체에 위치하는 5 개의 초위성체 마커(INRA30, TGLA325, UMN0803, UMN0905, UMN0929) 를 이용하여 재래소 3품종과 외래소 7품종(칡소, 한우, 제주흑우, 홀스타인, 일본화우, 샤롤레, 앵거스, 헤어포드, 시멘탈, 한우X 샤롤레 교잡종)의 유전적 특징을 확인하였다. 상업적으로 판매되는 소고기의 잘못된 원산지 표기를 통해 부당한 경제적 이득을 취하고자 하는 문제를 해결하기 위한 방법으로 소고기 샘플을 빠르고 저비용으로 확인 하기 위한 방법으로 사용하기 위해 좌위 또는 품종 특이적 대립유전자를 탐색하고 좌위별 대립유전자수, 대립유전자빈도, 이형접합도 그리고 다형정보량(PIC)을 구하여 이들 10품종의 유전적 다양성을 평가하였다. STRUCTURE 분석을 통한 군락의 분류 및 유전적 균일성 분석에서 재래소 품종과 외래소 품종으로 두개의 주요 그룹으로 나뉘어진다. 이러한 결과들은 재래소와 외래소 품종의 특이적인 유전적 차이를 나타낸다. 또한 Nei's 표준 유전적 거리로 나타난 neighbor-joining tree에서도 독립적인 계통유전학적인 위치를 보여주었다. 이러한 결과는 국내 재래종과 외래품종 사이의 유전적 거리, 품종의 역사 및 그들의 지리적 기원 사이에 명백한 차이를 나타내는 증거로 사료된다. 이러한 결과들로 이들 성염색체의 초위성체 마커들에 의해 소 품종들의 유전적 다양성과 연관성은 과학적인 기초자료로 활용되고 재래소와 외래품종 소고기를 구별할 수 있는 DNA 마커들로 이용될 수 있을 것으로 사료된다. 그러므로 이러한 마커들은 효율적인 이력추적 시스템을 만드는데 사용되어 원산지 표시 위반을 억제하는데 유용할 것이다.

• Journal of Plant Biotechnology
• /
• 제43권3호
• /
• pp.367-375
• /
• 2016

국내에서 육성된 옥수수 90 품종 및 자식 계통에 대하여 microsatellite 마커를 활용하여 DNA 프로파일 데이터베이스를 구축한 다음 공시품종에 따른 유전적 유사도 분석 및 품종식별력 검정에 대한 연구를 수행하였다. 옥수수 90품종을 100개의 microsatellite 마커로 검정하고 대립유전자의 패턴이 우수하고 다형성 정도가 높은 13개를 선정하여 분석하였을 때 대립유전자의 수는 5 ~ 24개까지 다양하게 분포하였고 평균 대립유전자의 수는 13.69개로 높았다. PIC 값의 경우도 0.716 ~ 0.942 범위에 속하였고 평균값은 0.865로 아주 높았다. 옥수수 90품종 및 계통에 대하여 UPGMA 분석에 의한 계통도를 작성하였을 때, 옥수수의 품종 유형 및 품종 육성 계보에 따라 5개의 대그룹으로 나누어졌다. 본 연구에서 구축됨 옥수수 자식계통 및 품종별 microsatellite DNA 프로파일 데이터베이스는 신품종과 기 육성된 품종과 유전적 유사도 분석이 가능하기 때문에 품종보호출원시 대조품종 선정 및 품종진위성과 관련된 종자분쟁에 매우 유용하게 활용될 수 있을 것이다.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 춘계학술발표회
• /
• pp.105-105
• /
• 2018

Finger millet, Eleusine coracana Gaertn., is more nutritious than other cereals and millets and widely cultivate in tropical regions of the world. However, status of its genetic diversity remained concealed due to lack of research work in this species. In recent years, microsatellites have become the most used markers for studying population genetic diversity. In present study, genetic diversity and structure of different populations of finger millet from Africa and South Asia was examined at molecular level using newly developed EST-Simple Sequence Repeat (EST-SSR) markers using a total of 1,927 ESTs of Eleusine coracana available in the NCBI database. In total, 46 primers produced 292 alleles in a size range of 100-500 bp and mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.372 and 1.04, respectively. 46 primers showed polymorphism and 21 primers were identified as having a PIC value above 0.5. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of finger millet accessions to their respective area of collection. The 156 accessions was classified into four groups, such as three groups of Africa collection and one group of Asia. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.

• Fisheries and Aquatic Sciences
• /
• 제26권12호
• /
• pp.752-761
• /
• 2023

Genetic diversity serves as the basis for selecting and genetically enhancing any culturable species in aquaculture. Here, two different strains of wild (River Ravi and River Kabul) and six captive-bred strains of Labeo rohita from various provinces were se- lected, and genetic diversity among them was evaluated using three different microsatellite markers, i.e., Lr-28, Lr-29, and Lr-37, and one mitochondrial CO1 (Cytochrome c oxidase subunit 1) gene. Different strains of L. rohita were collected, and part of their caudal fin was cut and preserved in ethanol for DNA extraction and determination of genetic diversity among them. Results in- dicated that selected markers were polymorphic with polymorphic information content (PIC) content values above 0.5 with the highest in Lr-28 followed by Lr-29 and then Lr-37. The observed heterozygosity (Ho) of all strains was higher (Avg: 0.731) but less than the expected heterozygosity (He). Moreover, TMs and WRs showed the highest He, while TKs showed the lowest, He. Over- all, inbreeding coefficient (FIS) values observed for all strains with selected markers were positive. The DNA barcoding with the CO1 gene revealed genetic variation among various strains, as demonstrated by the clades in the phylogenetic tree separating the strains into two distinct clusters that then divided into sub-clusters. In conclusion, TMs showed the highest heterozygosity as compared to other strains. Overall results provide the baseline data for the initiation of the genetic improvement program.

• 한국약용작물학회지
• /
• 제19권5호
• /
• pp.347-353
• /
• 2011

In this study, simple sequence repeat (SSR) analyses were utilized for evaluation of genetic diversity and discrimination of 17 accessions. Five cultivars, which were developed from Korea, and 12 foreign accessions, which were collected from China, Japan, Russia and USA, were evaluated by nine markers out of 22 SSR markers. A total of 39 alleles were detected, ranging from 2 to 8, with an average of 4.3 alleles per locus. The expected heterozygosity and PIC values were 0.627 and 0.553, with a range from 0.21 (GB-PG-078) to 0.76 (GB-PG-142) and from 0.19 (GB-PG-078) to 0.70 (GB-PG-142), respectively. Four makers out of nine SSR markers, GB-PG-026, GB-PG-043, GB-PG-142 and GB-PG-177, were selected as key factors for discrimination of Korean ginseng cultivars and foreign accessions. All of Korean ginseng cultivars and foreign accessions were individually by the combination of four SSR markers. Consequently, the SSR markers developed in this study may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and foreign accessions.