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http://dx.doi.org/10.5352/JLS.2017.27.4.464

Development of Microsatellite Markers for Discriminating Native Korean and Imported Cattle Breeds  

Kim, Seungchang (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Cho, Chang-Yeon (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Roh, Hee-Jong (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Yeon, Seong-Heum (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Choi, Seong-Bok (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
Publication Information
Journal of Life Science / v.27, no.4, 2017 , pp. 464-470 More about this Journal
Abstract
Three Korean native cattle (KNC) and seven exotic breeds (Chikso, Hanwoo, Jeju black, Holstein, Japanese black, Charolais, Angus, Hereford, Simmental, and Cross breed) were characterized by using five microsatellite (MS) markers (INRA30, TGLA325, UMN0803, UMN0905, and UMN0929) from the sex chromosome. Genetic diversity was evaluated across the 10 breeds by using the number of alleles per locus, allele frequency, heterozygosity, and polymorphism information content (PIC) to search for locus and/or breed specific alleles, allowing a rapid and cost-effective identification of cattle samples, avoiding mislabeling of commercial beef. It was divided into two main groups from STRUCTURE analysis, one corresponding to KNC and the other to exotic cattle breeds. These results also showed specific genetic differences between KNC and exotic breeds. Nei's standard genetic distance was calculated and used in the construction of a neighbor-joining tree. Results evidenced a correspondence between genetic distance, breeds' history, and their geographic origin, and a clear separation between KNC and exotic breeds. Overall, this study evidenced that DNA markers can discriminate between domestic and imported beef, contributing to the knowledge on cattle breeds' genetic diversity and relationships by using MS markers of the sex chromosome. These markers would be useful for inhibitory effect about false sales and for building an effective tracking system.
Keywords
Heterozygosity; Korean native cattle; microsatellite markers; polymorphism information content; sex chromosome;
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