Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Assessment of genetic diversity among wild and captive-bred Labeo rohita through microsatellite markers and mitochondrial DNA

  • Muhammad Noorullah (Fisheries & Aquaculture Program, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad) ;
  • Amina Zuberi (Fisheries & Aquaculture Program, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad) ;
  • Muhib Zaman (Fisheries & Aquaculture Program, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad) ;
  • Waqar Younas (Fisheries & Aquaculture Program, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad) ;
  • Sadam Hussain (Carp Hatchery and Training Center) ;
  • Muhammad Kamran (Aquaculture Laboratory, Department of Zoology, University of Sialkot)
  • Received : 2023.04.06
  • Accepted : 2023.10.17
  • Published : 2023.12.31
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Abstract

Genetic diversity serves as the basis for selecting and genetically enhancing any culturable species in aquaculture. Here, two different strains of wild (River Ravi and River Kabul) and six captive-bred strains of Labeo rohita from various provinces were se- lected, and genetic diversity among them was evaluated using three different microsatellite markers, i.e., Lr-28, Lr-29, and Lr-37, and one mitochondrial CO1 (Cytochrome c oxidase subunit 1) gene. Different strains of L. rohita were collected, and part of their caudal fin was cut and preserved in ethanol for DNA extraction and determination of genetic diversity among them. Results in- dicated that selected markers were polymorphic with polymorphic information content (PIC) content values above 0.5 with the highest in Lr-28 followed by Lr-29 and then Lr-37. The observed heterozygosity (Ho) of all strains was higher (Avg: 0.731) but less than the expected heterozygosity (He). Moreover, TMs and WRs showed the highest He, while TKs showed the lowest, He. Over- all, inbreeding coefficient (FIS) values observed for all strains with selected markers were positive. The DNA barcoding with the CO1 gene revealed genetic variation among various strains, as demonstrated by the clades in the phylogenetic tree separating the strains into two distinct clusters that then divided into sub-clusters. In conclusion, TMs showed the highest heterozygosity as compared to other strains. Overall results provide the baseline data for the initiation of the genetic improvement program.

Keywords

Acknowledgement

The staff at various hatcheries and training centers have contributed significantly to this study, and the authors would like to acknowledge their efforts. These include the Tawakkal hatchery in district Muzaffargarh, Mianchannu fish hatchery in district Khanewal, Punjab; the Upper Sindh hatchery in the Fisheries Department of the Government of Sindh; Carp hatchery and training center in Peshawar, Tanda fish hatchery in Kohat, and Charbanda fish hatchery in Mardan, KPK.

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