• 한국수정란이식학회지
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• 제32권3호
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• pp.147-151
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• 2017

Commercial applications of OPU/IVP were to produce embryos and calves from high genetic cows. The aim of this present study was to compare the number of recovered oocytes and cultured In vitro produced embryos from Ovum Pick-up (OPU). OPU derived embryo production was carried out of oocytes by ultrasonographic guided follicular aspiration and then produced in vitro produced blastocysts by IVP culture system. In result, the rate of recovered oocytes was obtained 612 (57.2%) and 451(73.7) G1+G2 grade oocytes. No difference of recovered rate (51.1~62.1%) was seen in six donor. The rate of cleavage and blastocyst development were obtained 320 (70.9%) and 78 (24.4%) that was $3.3{\pm}0.4$ cleaved embryo and $0.9{\pm}0.2$ blastocysts per session. Cleavage rate of OPU oocytes in No. 6 donor was 90.6%, significantly (P<0.05) higher than that in the other donors, However, blastocysts was similar (25.8~30.0%). In conclusion, limited numbers of OPU oocytes had competent development when cultured in SOF culture medium.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 21세기 한반도 농업전망과 대책(한국작물학회.한국육종학회 공동주관 심포지움 회보)
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• pp.336-352
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• 1998

The horticultural seed industry has made great progress in the last fifty years of its history after the independence of this country. The industry has accomplished self-sufficiency in major vegetable seeds and has even gained international competitive edge in certain crop seeds, particularly in hybrid cultivar development. However, the industry is facing a crisis at present coming from excessive competition among the domestic seed companies as well as the foreign currency crisis of the country. Several major seed companies have already been acquired by multinational corporations. Many people in the country as well as agriculturists are concerned about this situation. Although it is true that the industry is undergoing hard times, this crisis can be turned into a chance for making new progress depending on the joint efforts of the groups responsible, i.e., private companies, public institutions, and policy makers. Therefore, we have to turn this crisis into an opportunity for self-reform and progress. We should not be discouraged or give up. We Propose some ideas for the Progress of the seed industry. Public institutions and policy makers should do such things in cooperation as 1) training human resources for future breeders, 2) securing a wide range of genetic resources and improvement of the management system, 3) basic science research including biotechnology, 4) releasing breeding stocks from germplasm enhancement through use of exotic accessions, 5) support for breeding region- or use-specific cultivars, and 6) collection of foreign agricultural information for breeding cultivars for overseas markets. Eventually the responsible group for the final development of commercial cultivars, production, sales and management is private companies. Therefore, private companies also should have to do their best to develop competitive cultivars with a missionary spirit and endeavor for both domestic and overseas markets in cooperation with public institutions. Management based on competition, professionalism and rationalism is also urged. We are going to conclude with optimism that we can make a new vault of progress once the private and public sectors closely cooperate for the development of Korean horticultural seed industry by overcoming this crisis.

• 한국작물학회지
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• 제21권1호
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• pp.71-81
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• 1976

맥주맥의 육종에 있어서 곡립형질의 효율적인 선발자료를 얻고자 2조대맥 10품종을 공시하여 곡립형질의 유전력, 유전적 획득량, 상관관계, 경로계수등을 통계 유전학적으로 분석한 결과를 요약하면 다음과 같다. 1. 곡립형질들 중에서 곡피 (H=0.886), 분상질(H=0.848), 곡립형성비수(H=0.823)등은 유전력이 높고, 수량(H=0.526), 곡립폭(H=0.420)등은 유전역이 비교적 낮았다. 2. 기특되는 선발효율은 곡피(52.7%), 분상질(31.8%), 선입률(23.0%), 등이 높고, 곡립장(6.2%), 곡립폭(3.7%), 곡립후(8.7%) 등이 낮았다. 3. 맥주의 품질을 위하여 선발의 대상이되는 실용형질중 천립중, 분상질 Protein간의 상관관계는 다소유의성이 있었으나 곡립형질과 수량간의 상관관계는 유의성이 없었다. 4. 천립중, 곡피 및 수량의 선발은 이들에 대하여 직접효과가 큰 곡립후를 이용하여 간접적으로 선발하는 것이 효율적인 것으로 보였다. 5. 분상질 및 Protein을 위한 선발은 곡립후, 곡피 등과 수량간의 부의 간접효과를 개선하고 수량의 직접효과를 향상시키는 것이 유리할 것으로 보였다.

• 한국작물학회지
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• 제64권4호
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• pp.432-440
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• 2019

Global climate change exerts adverse effects on maize production. Among abiotic stresses, drought stress during the tasseling stage (VT) can increase anthesis-silking intervals (ASI) and decrease yield. We performed an evaluation of ASI and yield using a drought-sensitive line (Ki3) and a drought-tolerant line (Ki11) to analyze the correlation with ASI and yield. Moreover, the de novo data of Ki11 were analyzed to find putative novel transcripts related todrought stress in tropical maize. A total of 182 transcripts, with a log2 ratio >1.5, were found by comparing drought conditions to a control. The top 40 transcripts of high expression levels in the de novo analysis were selected and analyzed with PCR. Of the 40 transcripts, six novel transcripts were detected by quantitative real-time PCR (qRT-PCR) using seedling and VT stage samples. Five transcripts (transcripts_1, 12, 34, 35, and 40) were up-regulated in the Ki11 shoot at seedling stage, and transcripts_1, 12, and 40 were up-regulated at the re-watering stage after 12 h of drought stress. The transcripts_32 and 34 were up-regulated at the VT stage. Hence, transcript_34 possibly plays a significant role in drought tolerance during the seedling and VT stages. The transcript_32 was identified as chloramphenicol acetyltransferase (CAT) by Pfam domain analysis. The function of the other transcripts remained unknown. Further characterization of these novel transcripts in genetic regulation will be of great value for the improvement of maize production.

• Journal of Applied Biological Chemistry
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• 제57권2호
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• pp.123-129
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• 2014

The antioxidant and antimicrobial activities of Camellia oleifera seed oil were studied. Four kinds of seed oil samples were prepared, crude oil and refined oil, extracted by cold pressing method (CPC, CPR), and organic solvent extraction (OSC, OSR). Antioxidant activity analysis was measured in 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, ferric reducing Ability of Plasma, and 2,2-diphenyl-1-picrylhydrazyl assays. Besides, the percentage of inhibition of red blood cells hemolysis induced by 2,2'-azobis(2-amidnopropane) dihydrochlorid, the lag time of LDL conjugated dienes formation in vitro, and the inhibitors of loss in tryptophan fluorescence were all used to estimate the antioxidant activity of the samples. The total phenolic contents (TPC) were detemined by Folin-Ciocalteu method. The TPC of the C. oleifera seed oils can be arranged in descending order: CPC ($1.9172{\mu}g/mL$) > OSC ($1.5218{\mu}g/mL$) > CPR ($1.0611{\mu}g/mL$) > OSR ($0.6782{\mu}g/mL$). And the oils were investigated for activity against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The results showed the antioxidant activity of crude oil by cold pressing method was stronger than others, and all oils did inhibit activity of the top three bacteria expert A. niger. The further significance of the study contributes to measure the antioxidant and antimicrobial activity of the potential health benefits by the different methods of preparation and the oil of C. oleifera seeds acting as free radical scavenger, pharmaceuticals and preservatives may offer some information in medicine and cosmetic not just in food field.

• 한국토양비료학회지
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• 제37권6호
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• pp.396-406
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• 2004

Current physical and chemical methodologies, conventionally used to clean up metal contaminated soils, are generally too expensive to apply in large hazardous waste sites including agricultural lands adjacent to closed or abandoned metal mines. Phytoremediation using plants to extract, sequester and detoxify environmental pollutants is one of the cost-effective and aesthetically-pleasing alternatives, compared with environmentally destructive remedial methods currently being practiced. But, phytoremediation has some limitations such as time consuming and low performance: in general, it is seasonally dependent and slower in removing metals than other methods, and metal accumulating plants are slow growers. Improvement of plants for metal tolerance, accumulation, and translocation using genetic engineering techniques recently opened up new possibilities for phytoremediation. In this paper, we have discussed about recent developments in conventional and genetically engineered phytoremediation. For the conventional phytoremediation, focuses are on the natural hyperaccumulator and the chemically assisted phytoremediation. Some pros and cons on the phytoremediation using transgenic plants, coupled with focusing on the mechanistic view points, are also discussed. It might be concluded that the transgenic plants will be effective tools in the practical application of phytoremediation especially for the highly contaminated soils but mechanisms involved should be deeply understood in advance.

• 원예과학기술지
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• 제33권1호
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• pp.83-92
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• 2015

xBrassicoraphanus line BB#5, a new synthetic intergeneric hybrid between Brassica rapa L. ssp. pekinensis and Raphanus sativus L. var. rafiphera induced by N-methyl-N-nitroso-urethane mutagenesis in microspore culture, shows high seed fertility and morphological uniformity. Dual-color fluorescence in situ hybridization (FISH) using 5S and 45S rDNA probes and genomic in situ hybridization (GISH) using B. rapa genomic DNA probe were carried out to analyze the chromosome composition and the meiosis pairing pattern compared to its parental lines. The somatic chromosome complement is 2n = 38, which consists of 17 metacentric and two submetacentric chromosomes with lengths of 2.18 to $5.01{\mu}m$. FISH karyotype analysis showed five and eight pairs of 5S and 45S rDNA loci. GISH meiosis pairing analysis showed that 19 complete bivalents were most frequent and accounted for 42% of the 100 pollen mother cells examined. Based on chromosome number, size, morphology, rDNA distribution, and meiosis pairing pattern, both parental genomes of B. rapa and R. sativus appear to exist in xBrassicoraphanus line BB#5, demonstrating its genome integrity. Such stable chromosome constitutions and meiotic pairing patterns in somatic and meiotic cells are very rare in natural and synthetic intergeneric hybrids. Chromosomal studies and genetic and phenotypic changes in allopolyploids a re discussed. The results p resented h erein will b e usef ul f or f urther g enomic s tudy o f xBrassicoraphanus lines and their improvement as promising new breeding varieties.

• 한국지능시스템학회논문지
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• 제21권3호
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• pp.401-406
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• 2011

복잡해진 최적화문제를 전통적인 방법보다 효율적으로 해결하기위해 유전알고리즘이나 개미군집화, 하모니서치알고리즘과 같은 다양한 메타휴리스틱이 개발되었다. 그 중에서 하모니 서치알고리즘이 다른 메타휴리스틱알고리즘보다 좋은 결과를 보이고 있다. 하모니 서치 알고리즘은 음악을 작곡할 때 아름다운 소리를 내는 하모니를 찾는 과정을 모방했다. 성능은 하모니 메모리에서 선택하는 비율인 HMCR값과 하모니 메모리에서 선택된 값의 조정 비율을 결정하는 PAR값에 따라 달라지는 것으로 알려져 있다. 다르게 말하면 두 변수의 기반이 되는 하모니 메모리의 사용방법의 문제로 볼 수 있다. 본 논문은 설정한 기간 동안 더 좋은 최적해를 찾지 못할 경우 하모니 메모리의 일부를 좋은 하모니로 구성되게 수정하는 방법을 제안했다. 테스트 함수를 이용한 검증 실험결과에서 하모니 메모리를 수정할 경우 정확도 변화가 적어 신뢰성 있는 정확도를 보였으며, Iteration이 짧더라도 최적값에 근접한 값을 찾았다.

• 한국정보통신학회논문지
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• 제11권7호
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• pp.1318-1324
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• 2007

지금까지의 상품 검색 방법으로는 찾고자하는 정보를 검색할 때 주로 단어의 빈도수나 어휘 정보를 이용하는 키워드 기반의 검색이 주로 쓰이고 있었다. 키워드 기반의 검색에서는 사용자의 질의와 관련이 없는 문서들까지도 같은 결과로 나타내 주고 이로 인해 사용자는 제시된 결과를 한번 더 수동적으로 검색해야하는 부담을 않게 되었다. 이러한 문제점을 해결하기 위해 온톨로지가 대두되었다. 본 논문에서는 온톨로지를 이용한 상품 검색 시스템을 직접 구축하여 분류별 검색을 통해 얼마나 정확한 검색을 하는지 실험하였다. 실험을 위해 전국적으로 On/Off라인 할인점을 운영 중에 있는 A할인점의 상품 데이터 약 40,000여개를 데이터 베이스로 구축하였고 User Interface 개발환경은 JSP와 PowerBuilder9.0을 사용하여 검색 시스템을 개발하여 실험하였다. 그 결과 본 논문에서 제안하고 설계한 상품 도메인 온톨로지를 이용한 검색 방법이 기존의 기반의 검색 방법보다 우수한 결과를 나타내고 있음을 입증하였다.

• Journal of Animal Science and Technology
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• 제63권4호
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• pp.759-765
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• 2021

Recently, summer temperatures have frequently been abnormal in Korea owing to global warming. In summer, a decrease in feed intake rate and biological activity were observed in Hanwoo (Korean Native Cattle), leading to lower production rates in the industry. However, the precise scale of damage was not reported as with other animals of economic value. This study was conducted to investigate the effects of birth season on birth weight in Hanwoo. Data were collected from 100 local breeding farms from 2016 to 2019. A total of 41,081 Hanwoo calves were classified and analyzed by sex, year, month, and season (March-May, spring; June-August, summer; September-November, fall; and December-February, winter) of birth. The birth weight of Hanwoo calves differed according to birth month. The average birth weight of male calves was 30.47 kg and that of female calves was 28.16 kg. Hanwoo birth weight was the highest in March-born calves and the lowest in July-born calves. The birth weights of calves born in February, March, April, November, and December were significantly larger than those of calves born in July. In addition, the birth weight of Hanwoo calves from the summer was significantly lower than that of calves born in other seasons. Furthermore, Hanwoo steer slaughter age showed a negative correlation, whereas carcass weight had a positive correlation with birth weight. In the beef cattle industry, birth weight is a very important economic characteristic that is related to growth rate. These data will contribute toward planning the reproduction of Hanwoo and analysis of changes in characteristics of economic value owing to high temperatures.