• 농업과학연구
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• 제45권2호
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• pp.238-247
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• 2018

The overall consumption of meat is increasing as the level of national income increases. The end weight is a trait closely associated with dressed meat. Genome-wide association study (GWAS) is an effective method of analyzing genetic variation and gene identification associated with a number of natural alternative traits because it can detect variations. So this paper did a GWAS analysis to identity the location on the genome related to the end weight in purebred landrace pigs and to explore the relevant candidate gene. This study identified a significant single nucleotide poly morphism (SNP) marker in chromosome 6 (ASGA0029422, $p=1.22{\times}10^{-6}$). Adhesion G protein-coupled receptor L2 (ADGRL2) was found to be the candidate gene at the identified SNP marker location. ADGRL2 genes have been found to be associated with cell development in relation to the external and internal environment of a cell. In addition, genotype and statistical analyses were done on nine variations on the exon of ADGRL2. The results show that the SNP marker (ASGA0029422, $p=1.32{\times}10^{-6}$) was significant, but the significance of the nine variations on the ADGRL2 exon was not verified. However, by performing further experiments and functional studies on other SNPs showing possible genetic ADGRL-Exon mutations, objects with high associations of high-end weights can be selected.

• The Plant Pathology Journal
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• 제34권6호
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• pp.532-543
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• 2018

Complete genome sequences of 22 isolates of Cucurbit aphid-borne yellows virus (CABYV), collected from melon plants showing yellowing symptom in Korea during the years 2013-2014, were determined and compared with previously reported CABYV genome sequences. The complete genomes were found to be 5,680-5,684 nucleotides in length and to encode six open reading frames (ORFs) that are separated into two regions by a non-coding internal region (IR) of 199 nucleotides. Their genomic organization is typical of the genus Polerovirus. Based on phylogenetic analyses of complete nucleotide (nt) sequences, CABYV isolates were divided into four groups: Asian, Mediterranean, Taiwanese, and R groups. The Korean CABYV isolates clustered with the Asian group with > 94% nt sequence identity. In contrast, the Korean CABYV isolates shared 87-89% sequence identities with the Mediterranean group, 88% with the Taiwanese group, 81-84% with the CABYV-R group, and 72% with another polerovirus, M.. Recombination analyses identified 24 recombination events (12 different recombination types) in the analyzed CABYV population. In the Korean CABYV isolates, four recombination types were detected from eight isolates. Two recombination types were detected in the IR and P3-P5 regions, respectively, which have been reported as hotspots for recombination of CABYV. This result suggests that recombination is an important evolutionary force in the genetic diversification of CABYV populations.

• 생명과학회지
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• 제25권6호
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• pp.624-630
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• 2015

본 연구는 microsatellite marker를 이용하여 국내에서 사육되고 있는 칡소 9지역 간의 유전적 거리 분석 및 계통 지도 작성 등의 계통유전학적 분석을 실시하였다. 11종의 MS 마커를 이용하여 대립유전자의 수(No. of allele)를 확인한 결과 8에서 24개로 확인되었으며, 기대이형접합율(expected heterozygosity, H_exp)은 0.672에서 0.834 범위 안에 나타났으며, 관측이형접합율(observed heterozygosity, H_obs)은 0.687에서 0.886, 다형성정보지수(Polymorphism information content, PIC)은 0.638에서 0.876로 확인되었다. 무작위 교배집단(Random)으로 가정하였을 경우 동일개체 출현빈도는 11개의 marker를 사용하였을 때, 5.24×10⁻¹⁹ 빈도로 출현하는 것을 확인 할 수 있었으며, 반형매 교배집단(Half-sib)과 전형매 교배집단(sib)으로 가정했을 경우에는 2.63×10⁻⁰⁶, 2.63×10⁻⁰⁶으로 각각 확인되었다. 이러한 결과는 칡소의 개체식별 및 친지확인 marker로 11종의 MS marker가 충분히 활용 가능할 것으로 사료된다. Phylogenetic tree (Neighbor-Joining tree), Principle Component Analysis (PCA) 그리고 Factorial Component Analysis (FCA) 분석을 통해 9 지역의 칡소 집단 간의 유연관계를 확인하였다. 이러한 결과는 칡소 품종을 중요한 가축유전자원으로써 인식하고 국내 타 품종과의 유전적 차별화와 순수성 보존과 능력을 개량하는데 있어 기초 자료로 활용 가능할 것으로 사료된다.

• 한국실내디자인학회논문집
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• 제19권2호
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• pp.99-106
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• 2010

This study is about Foucault's conceptual and methodological relevance for structural analysis of Korean traditional space design history. By Foucault's Theory, regionalism is product of the instrumentality of space, power, knowledge and provide the basis for making the operation of power both spatial and temporal. The purpose of this study is to establish topographical map of historical progress and to shed new light on the forming of identity in Korean traditional space design on the poststructural-archaeological aspect. The process of this study is illustrated as follows: At first, it suggests Foucault's 'Theory of archaeology'. It mentions 'historical apriori' and archive, collateral space and general grammar as theoretical background of archaeological logic. Secondly, it clarifies 'statements' depending on the conditions that is 'episteme' in which they emerge and exist within a field of discourse. And it discuss Korean traditional space design concept as a social product on the situated character of interaction in time-space. Finally, it studies genetic variation process of Korean traditional space design based on the social progress in Korean society. And it analyses practical actions of 'Statements' in terms of general grammar and textuality.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.652-655
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• 2001

The comparative study of enzymes that catalyze a similar reactions but have different substrate spectrum and/or stereospecificity is a powerful approach to understanding the reaction mechanism between the relative enzymes, and it was also an useful tool to cloning the related enzyme, without the typical cloning from DNA library of genomic pools. For this purpose, we conducted an approach that the comparison at the molecular and protein level of esterases, from various sources including a previously identified (S)-stereospecific esterase of Pseudomonas sp. ES1. As expected, we found an esterase family genes that shared a high similarity at the protein and genetic level in the identical genus Pseudomonad. The striking structural and biochemical identity strongly suggested the family genes to be an identical one. We, hence, aligned the family genes and designated a degenerated primer for PCR-cloning using six Pseudomonas strains as templates. As a result, a recombinant esterase from Pseudomonas fluorescens KCTC 1767 was cloned and high-level expressed with high selectivity to (R,S)-ketoprofen ethyl ester. The enzyme exhibited a high ester-hydrolyzing activity to (S)-ketoprofen but did not hydrolyzed the opposite stereoisomer. Further characteristics were discussed in our presentation.

• 미생물학회지
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• 제34권3호
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• pp.115-119
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• 1998

Pseudomonas sp. DJ77로부터 전보에서 클로닝한 pHENX7의 하류방향으로 약 3kb 정도를 포함하는 pYCS500을 클로닝하였다. PYCS500의 제한효소지도를 작성하고 부분적으로 염기서열을 분석한 결과 465 bp의 HindIII-ClaI절편에서 282 bp로 이루어진 하나의 open reading frame(ORF)을 발견하였다. phnM으로 명명된 이 ORF는 93개의 아미노산으로 구성된 polypeptide를 암호화하고 있었으며 계산된 분자량은 10,008 Da이었다. PhnM은 NahT, XylT, DmpQ, AtdS, PhlG, PhhQ, TbuW 등 plant-type ferredoxin 형태의 단백질과 37.7%-53.9%의 상동성을 나타내었으며 이들이 공통적으로 가지고 있는 motif가 일치하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.13-16
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• 2001

Bovine ${\beta}1$-adrenergic receptor (AR) cDNA was cloned using degenerative primers. Bovine ${\beta}1$-AR coded for 467 amino acids and the comparison of the deduced amino acid sequence with that of sheep showed 93.4% identity. Northern blot analysis indicated that transcript size for the bovine ${\beta}1$-AR was 3.6 kb in the adipose tissue. The expression level of three $\beta$-ARs (1, 2, and 3) in bovine abdominal, subcutaneous, and perirenal adipose tissues were examined using reverse transcription-polymerase chain reaction (RT-PCR), and the levels of ${\beta}1$- and ${\beta}3$-AR mRNA were found to be lower in the subcutaneous adipose tissue than in the abdominal and perirenal adipose tissues. These results suggest that the expression of $\beta$-ARs mRNA are differentially regulated among the adipose tissues.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1534-1538
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• 2005

In this paper, we propose a method to detect contours of a face, eyes, and a mouth of a person in the color image in order to make an avatar automatically. First, we use the HSI color model to exclude the effect of various light conditions, and find skin regions in the input image by using the skin color defined on HS-plane. And then, we use deformable templates and genetic algorithm (GA) to detect contours of a face, eyes, and a mouth. Deformable templates consist of B-spline curves and control point vectors. Those represent various shapes of a face, eyes and a mouth. GA is a very useful search algorithm based on the principals of natural selection and genetics. Second, the avatar is automatically created by using GA-detected contours and Fuzzy C-Means clustering (FCM). FCM is used to reduce the number of face colors. In result, we could create avatars which look like handmade caricatures representing user's identity. Our approach differs from those generated by existing methods.

• ALGAE
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• 제32권3호
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• pp.235-244
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• 2017

Freshwater algae living in shallow waters have evolved various photomovement to stay in the optimum light condition for survival. Previous action-spectra investigations showed that Spirogyra filaments have phototropic movement in blue light. To decipher the genetic control of phototropic movement, two phototropin homologues were isolated from Spirogyra varians, and named SvphotA and SvphotB. Both phototropins have similar molecular structure consisted of two light-oxygen-voltage domains (LOV1, LOV2) and a serine / threonine kinase domain. SvphotA and SvphotB had 48.7% sequence identity. Phylogenetic analysis showed SvphotA and SvphotB belong to different clades suggesting early divergence, possibly before the divergence of land plants from the Zygnematales. Quantitative PCR and northern blot analysis showed that SvphotA and SvphotB responded differently to red and blue light. SvphotA was consistently expressed in the dark and in blue light, while SvphotB was expressed only when the plants were exposed to light. When the filaments were exposed to red light, SvphotA was significantly downregulated whereas SvphotB was highly upregulated. These results suggest that the two phototropins may have different roles in the photoresponse in S. varians.

• 식물병연구
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• 제20권3호
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• pp.219-222
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• 2014

Several tomato production regions in Korea were surveyed for tomato yellow leaf curl disease (TYLCD). Tomato leaf samples showing TYLCD-like symptoms were collected from Tongyeong (To), Geoje (Gi), and Gimhae (Gh) cities of the southern part of Korea. Tomato yellow leaf curl virus (TYLCV) was detected and the full-length genomes of the isolates were sequenced. The TYLCV isolates found in Korea shared high sequence identity (> 99%) with TYLCV-IL [JR:Omu:Ng] (AB110217). Phylogenetic relationship analysis revealed that they formed two groups (with little genetic variability), and the To, Gj, and Gh isolates belonged to the TYLCV-IL group. An infectious clone of TYLCV-To (JQ013089) was constructed and agroinoculated into Nicotiana benthamiana, Nicotiana tabacum var. Xanthi, Petunia hybrida, Capsicum annuum, and Lycopersicon esculentum cv. Hausumomotaro. Agroinfection with a dimeric infectious clone of TYLCV-To induced severe leaf curling and stunting symptoms in these plants, excluding C. annuum. Tomato plants then developed typical yellow leaf curl symptoms.