• Clinical and Experimental Reproductive Medicine
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• 제22권3호
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• pp.279-285
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• 1995

Despite the frequent incidence of embryo fragmentation in early human embryos, the reason of the embryo fragmentation has not been known yet. This study was conducted to investigate the histological difference(s) between fragmented (FR) and non-fragmented (NFR) human embryos focusing on comparison of mitochondrial distribution and protein synthesis. Multi-pronuclei zygotes (MPZ) such as three or more pronuclei containing in human in vitro fertilization and embryo transfer (IVF-ET) program were used for this study. MPZ were cultured in TCM-199 supplemented with 10% of human fetal cord serum (hFCS) in 5% $CO_2$ incubator at $37^{\circ}C$ for 24 hours. The cleaved embryos to 2-4 cells after 24 hours were grouped by their grade of fragmentation. Embryos were stained with Rhodamine123 (Rh123) and fluorescence was evaluated under the fluorescence microscope through PB 450-490 filter (Leitz). Regarding to protein synthesis during early human embryogenesis, there is no significant difference in the amount of synthetic proteins between FR and NFR embryos. Distribution of cytoplasmic organelles in embryos was evaluated by transmission electron microscope (TEM). The cytoplasmic distribution of mitochondria was different between FR and NFR embryos. The mitochondrial distribution was even in NFR, whereas severely aggregated in FR. It is not able to clarify in the present study whether this uneven mitochondrial distribution in FR embryo is the reason for embryo fragmentation or is the result from fragmentation. Physiological disparity related to the mitochondrial distribution may be one of the reasons for embryo fragmentation. Further studies should be addressed to investigate the physiological differences between FR and NFR embryos.

• 대한수학회지
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• 제39권4호
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• pp.543-558
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• 2002

We investigate the fragmentation process developed by Kolmogorov and Filippov, which has been studied extensively by many physicists (independently for some time). One of the most interesting phenomena is the shattering (or disintegration of mass) transition which is considered a counterpart of the well known gelation phenomenon in the coagulation process. Though no masses are subtracted from the system during the break-up process, the total mass decreases in finite time. The occurrence of shattering transition is explained as due to the decomposition of the mass into an infinite number of particles of zero mass. It is known only that shattering phenomena occur for some special types of break-up rates. In this paper, by considering the n-particle system of stochastic fragmentation processes, we find general conditions of the rates which guarantee the occurrence of the shattering transition.

• 정보처리학회논문지A
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• 제8A권4호
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• pp.331-338
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• 2001

네트워크가 보편화되면서 사이버 공간을 이용한 테러가 전 세계적으로 발생하고 있다. IP Fragmentation은 이 기종 네트워크 환경에서 IP 패킷의 효율적인 전송을 보장해주고 있지만, 몇 가지 보안 문제점을 가지고 있다. 불법 침입자는 이러한 IP Fragmentation의 취약점을 이용해 IP Spoofing, Ping of Death, ICMP 등의 공격 기술을 이용하여 시스템에 불법적으로 침입하거나 시스템의 정상적인 동작을 방해한다. 최근에는 IP Fragmentation을 이용한 서비스 거부공격 외에도 이를 이용하여 패킷 필터링 장비나 네트워크 기반의 침입탐지시스템을 우회할 수 있는 문제점이 대두되고 있다. 본 논문에서는 패킷 재 조합 기능을 제공하지 못하는 일부 라우터나 네트워크 기반의 침입탐지시스템들에서 불법 사용자가 패킷을 다수의 데이터 그램으로 나누어 공격할 때, 이를 탐지하거나 차단하지 못하는 경우에 대비하여 실시간 접근 로그 파일을 생성하고, 시스템 관리자가 의사결정을 할 수 있도록 함과 동시에 시스템 스스로 대처할 수 있는 시스템을 구현하여 타당성을 검증하고, 그에 따른 기대효과를 제시하고자 한다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2001년도 봄 학술발표회 논문집
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• pp.129-136
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• 2001

Blasting method is used most engineering works for rock excavation. Blasting method is done much to upgrade of operation efficiency, contraction of construction period than other method. But blasting method happens damage by blasting vibration, nose and scattering. Therefore this study examined about effect, characteristic and application of Plasma method. To confirm effect measured vibration, noise and frequency, and analyzed data compare with general blasting.

• Mass Spectrometry Letters
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• 제4권4호
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• pp.79-82
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• 2013

Various types of alkaloids observed in several herbal medicines were analyzed by electrospray ionization tandem mass spectrometry in positive ion mode. In the present study, MS/MS spectralpatterns were investigated for eight-types of alkaloids (aporpine, protoberberine, tetrahydroprotoberberine, benzylisoquinoline, protopine, phthalide, morpine, and bisbenzylisoquinoline). For aporpine- and protoberberine-type alkaloids, main fragmentations occurred at substituted groups on rigid ring structures, not showing ring fusion. Interesting fragmentations due to iminolization and retro-Diels-Alder (RDA) reaction were observed in MS/MS spectra of protopine- and tetrahydroprotobereberine-type alkaloids. Also, several types of fragmentations such as inductive cleavage and ${\alpha}$-cleavage, or bond cleavage between two ring structures were observed depending on their structural characteristics. These fragmentation patterns are expected to allow instant classification of the specific alkaloid type in various MS/MS spectra of alkaloids.

• Clinical and Experimental Reproductive Medicine
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• 제49권1호
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• pp.40-48
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• 2022

Objective: As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.

• International Journal of Stem Cells
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• 제16권2호
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• pp.123-134
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• 2023

Objective: The heart contains a pool of c-kit⁺ progenitor cells which is believed to be able to regenerate. The differentiation of these progenitor cells is reliant on different physiological cues. Unraveling the underlying signals to direct differentiation of progenitor cells will be beneficial in controlling progenitor cell fate. In this regard, the role of the mitochondria in mediating cardiac progenitor cell fate remains unclear. Specifically, the association between changes in mitochondrial morphology with the differentiation status of c-kit⁺ CPCs remains elusive. In this study, we investigated the relationship between mitochondrial morphology and the differentiation status of c-kit⁺ progenitor cells. Methods and Results: c-kit⁺ CPCs were isolated from 2-month-old male wild-type FVB mice. To activate differentiation, CPCs were incubated in α-minimal essential medium containing 10 nM dexamethasone for up to 7 days. To inhibit Drp1-mediated mitochondrial fragmentation, either 10 μM or 50 μM mdivi-1 was administered once at Day 0 and again at Day 2 of differentiation. To inhibit calcineurin, either 1 μM or 5 μM ciclosporin-A (CsA) was administered once at Day 0 and again at Day 2 of differentiation. Dexamethasone-induced differentiation of c-kit⁺ progenitor cells is aligned with fragmentation of the mitochondria via a calcineurin-Drp1 pathway. Pharmacologically inhibiting mitochondrial fragmentation retains the undifferentiated state of the c-kit⁺ progenitor cells. Conclusions: The findings from this study provide an alternative view of the role of mitochondrial fusion-fission in the differentiation of cardiac progenitor cells and the potential of pharmacologically manipulating the mitochondria to direct progenitor cell fate.

• 화약ㆍ발파
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• 제29권2호
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• pp.13-31
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• 2011

국내외의 광산 및 대규모 현장에서는 노천발파 시, Air Deck 발파공법을 사용하여 발파효율성을 높이려 하고 있으나 이는 공 내부 상태에 따라 장전밀도가 달라짐으로 인해 대괴가 발생하는 등 문제점이 있다. 특히 석회석 광산의 경우 침식 용해 작용에 의해 공벽이 확공되거나 손상된 경우가 많아 Air Deck 공법 적용 시 의도하지 않은 집중장약이 발생하는 경우가 있다. 따라서 본 연구에서는 석회석 광산의 대규모 노천발파를 대상으로 사전에 공 내부를 내시경 검사한 후 공 내부에서 장약집중을 야기할 수 있는 구간을 Air Tube를 이용한 데크차지 공법으로 분상장약(Deck Charge)하여 Air Deck 발파공법의 효과가 온전히 나타나도록 유도하였고 이를 일반장약 공법과 비교하였다. 비교대상은 전체적인 발파효과, 파쇄도, 장약량 절감율 및 시공속도(장약에서 발파까지의 총 작업속도)였으며, 그 결과 시공속도를 제외한 나머지 비교대상에 있어 일반장약에 의한 발파공법 보다 Air Deck를 이용한 이중분상 발파공법이 더 효율적으로 나타났다.

• 한국자동차공학회논문집
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• 제10권4호
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• pp.149-157
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• 2002

This paper is to investigate collapse mechanisms of CFRP(Carbon Fiber Reinforced Plastics)composite tubes and to evaluate collapse characteristics on the change of interlaiminar number and ply orientation angle of outer under static and impact axial compression loads. When a CFRP composite tube is crushed, static/impact energy is consumed by friction between the loading plate and the splayed fronds of the tube, by fracture of the fibers, matrix and their interface. These are associated with the energy absorption capability. In general, CFRP tube with 6 interlaminar number(C-type), absorbed more energy than other tubes(A, B, D-types). The maximum collapse load seemed to increase as the interlaminar number of such tubes increases. The collapse mode depended upon orientation angle of outer of CFRP tubes and loading status(static/impact). Typical collapse modes of CFRP tubes are wedge collapse mode, splaying collapse mode and fragmentation collapse mode. The wedge collapse mode was shown in case of CFRP tubes with 0° orientation angle of outer under static and impact loadings. The splaying collapse mode was shown in only case of CFRP tubes with 90°orientation angle of outer under static loadings, however in Impact tests those were collapsed in fragmentation mode .

• 수면정신생리
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• 제4권2호
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• pp.140-146
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• 1997

Sleep-related breathing disorders, especially sleep apnea syndrome are complicated by neuropsychiatric dysfunction such as excessive daytime sleepiness, cognitive dysfunction, and depression. As the determinants of daytime sleepiness, sleep fragmentation is more influential than nocturnal hypoxia. Daytime sleepiness can be improved by continuous positive airway pressure (CPAP) or surgery in up to 95% of the treated subjects. Both sleepiness and nocturnal hypoxia would cause cognitive dysfunction. While impairments in attention and verbal memory are more related with sleepiness and prominent in mild to moderate sleep apnea syndrome (SAS), impairments in general intellectual function and executive function are more related with nocturnal hypoxia and prominent in severe SAS. Several cognitive deficits related with nocturnal hypoxia may be irreversible despite CPAP or surgical treatments. So, early detection and early appropriate treatment of SAS would prevent sleepiness and cognitive deterioration.