Browse > Article
http://dx.doi.org/10.5653/cerm.2021.04777

Correlations between abnormalities of morphological details and DNA fragmentation in human sperm  

Nguyen, Hiep Tuyet Thi (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University)
Dang, Hong Nhan Thi (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University)
Nguyen, Thai Thanh Thi (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University)
Nguyen, Trung Van (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University)
Dang, Thuan Cong (Department of Histology, Embryology, and Pathology, Hue University of Medicine and Pharmacy, Hue University)
Nguyen, Quoc Huy Vu (Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University)
Le, Minh Tam (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.49, no.1, 2022 , pp. 40-48 More about this Journal
Abstract
Objective: As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.
Keywords
Anatomy and histology; DNA fragmentation; Infertility; Male; Spermatozoa;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Green KA, Patounakis G, Dougherty MP, Werner MD, Scott RT Jr, Franasiak JM. Sperm DNA fragmentation on the day of fertilization is not associated with embryologic or clinical outcomes after IVF/ICSI. J Assist Reprod Genet 2020;37:71-6.   DOI
2 Alvarez Sedo C, Bilinski M, Lorenzi D, Uriondo H, Noblia F, Longobucco V, et al. Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects. JBRA Assist Reprod 2017;21:343-50.
3 Eidenberger F, Huber KL, Fuchs P, Simkovicova N, Imhof M. Measured with sperm chromatin dispersion (SCD) technique: will DNA integrity in spermatozoa increase after micronutrient supplementation? Comparative controlled study. Fertil Reprod 2019;1:136-40.   DOI
4 Dam AH, Feenstra I, Westphal JR, Ramos L, van Golde RJ, Kremer JA. Globozoospermia revisited. Hum Reprod Update 2007;13:63-75.   DOI
5 Zhang ZQ, Long SG, Huang ZH, Xin CL, Wu QF. Different outcomes after intracytoplasmic sperm injection without oocyte activation in two patients with different types of globozoospermia. Andrologia 2016;48:116-20.   DOI
6 Modarres P, Tavalaee M, Ghaedi K, Nasr-Esfahani MH. An overview of the globozoospermia as a multigenic identified syndrome. Int J Fertil Steril 2019;12:273-7.
7 Eskandari N, Tavalaee M, Zohrabi D, Nasr-Esfahani MH. Association between total globozoospermia and sperm chromatin defects. Andrologia 2018;350:e12843.
8 Belloc S, Benkhalifa M, Cohen-Bacrie M, Dalleac A, Chahine H, Amar E, et al. Which isolated sperm abnormality is most related to sperm DNA damage in men presenting for infertility evaluation. J Assist Reprod Genet 2014;31:527-32.   DOI
9 Sakkas D, Seli E, Bizzaro D, Tarozzi N, Manicardi GC. Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis. Reprod Biomed Online 2003;7:428-32.   DOI
10 Sivanarayana T, Krishna C, Prakash GJ, Krishna KM, Madan K, Rani BS, et al. CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation. J Assist Reprod Genet 2012;29:1327-34.   DOI
11 Agarwal A, Majzoub A, Baskaran S, Panner Selvam MK, Cho CL, Henkel R, et al. Sperm DNA fragmentation: a new guideline for clinicians. World J Mens Health 2020;38:412-71.   DOI
12 Ribas-Maynou J, Yeste M. Oxidative stress in male infertility: causes, effects in assisted reproductive techniques, and protective support of antioxidants. Biology (Basel) 2020;9:77.   DOI
13 Kullander S, Rausing A. On round-headed human spermatozoa. Int J Fertil 1975;20:33-40.
14 Cito G, Picone R, Fucci R, Giachini C, Micelli E, Cocci A, et al. Sperm morphology: what implications on the assisted reproductive outcomes? Andrology 2020;8:1867-74.   DOI
15 Xie D, Lu C, Zhu Y, Zhu S, Yang EJ, Jin X. Analysis on the association between sperm DNA fragmentation index and conventional semen parameters, blood microelements and seminal plasma ROS in male patients with infertility. Exp Ther Med 2018;15:5173-6.
16 Ferrigno A, Ruvolo G, Capra G, Serra N, Bosco L. Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients. J Assist Reprod Genet 2021;38:979-86.   DOI
17 Jakubik-Uljasz J, Gill K, Rosiak-Gill A, Piasecka M. Relationship between sperm morphology and sperm DNA dispersion. Transl Androl Urol 2020;9:405-15.   DOI
18 Daris B, Goropevsek A, Hojnik N, Vlaisavljevic V. Sperm morphological abnormalities as indicators of DNA fragmentation and fertilization in ICSI. Arch Gynecol Obstet 2010;281:363-7.   DOI
19 Oehninger S, Kruger TF. Sperm morphology and its disorders in the context of infertility. F S Rev 2021;2:75-92.
20 Sharbatoghli M, Valojerdi MR, Amanlou M, Khosravi F, Jafar-abadi MA. Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection. Arch Gynecol Obstet 2012;286:1315-22.   DOI
21 Broussard AL, Leader B, Tirado EE, Russell H, Beydoun HA, Colver R, et al. High sperm DNA fragmentation index (DFI) on the day of oocyte retrieval is correlated with lower fertilization and slower development to the blastocyst stage for trophectoderm biopsy. Fertil Steril 2018;110:E295.
22 Samplaski MK, Dimitromanolakis A, Lo KC, Grober ED, Mullen B, Garbens A, et al. The relationship between sperm viability and DNA fragmentation rates. Reprod Biol Endocrinol 2015;13:42.   DOI
23 Kochhar PK, Ghosh P. Intracytoplasmic sperm injection with assisted oocyte activation resulting in successful pregnancies and live birth in couples with globozoospermia: a report of two cases. J Hum Reprod Sci 2018;11:72-4.   DOI
24 Dam AH, Ramos L, Dijkman HB, Woestenenk R, Robben H, van den Hoven L, et al. Morphology of partial globozoospermia. J Androl 2011;32:199-206.   DOI
25 Gunes S, Al-Sadaan M, Agarwal A. Spermatogenesis, DNA damage and DNA repair mechanisms in male infertility. Reprod Biomed Online 2015;31:309-19.   DOI
26 Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reprod Biol Endocrinol 2015;13:37.   DOI
27 Agarwal A, Majzoub A, Parekh N, Henkel R. A schematic overview of the current status of male infertility practice. World J Mens Health 2020;38:308-22.   DOI
28 World Health Organization. Laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization; 2010.
29 Marchetti F, Bishop J, Gingerich J, Wyrobek AJ. Meiotic interstrand DNA damage escapes paternal repair and causes chromosomal aberrations in the zygote by maternal misrepair. Sci Rep 2015;5:7689.   DOI
30 Mostafa RM, Nasrallah YS, Hassan MM, Farrag AF, Majzoub A, Agarwal A. The effect of cigarette smoking on human seminal parameters, sperm chromatin structure and condensation. Andrologia 2018;50:e12910.   DOI
31 Bosco L, Notari T, Ruvolo G, Roccheri MC, Martino C, Chiappetta R, et al. Sperm DNA fragmentation: an early and reliable marker of air pollution. Environ Toxicol Pharmacol 2018;58:243-9.   DOI
32 Oumaima A, Tesnim A, Zohra H, Amira S, Ines Z, Sana C, et al. Investigation on the origin of sperm morphological defects: oxidative attacks, chromatin immaturity, and DNA fragmentation. Environ Sci Pollut Res Int 2018;25:13775-86.   DOI
33 Sivanarayana T, Ravi Krishna C, Jaya Prakash G, Krishna KM, Madan K, Sudhakar G, et al. Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection. Reprod Med Biol 2013;13:87-94.   DOI
34 Aboulmaouahib S, Madkour A, Kaarouch I, Sefrioui O, Saadani B, Copin H, et al. Impact of alcohol and cigarette smoking consumption in male fertility potential: looks at lipid peroxidation, enzymatic antioxidant activities and sperm DNA damage. Andrologia 2018;50:e12926.   DOI
35 Karabulut A, Tekin A. Alterations in the morphology and motility of spermatozoa: relation with total sperm count. Pam Med J 2013;6:1-4.   DOI
36 Agarwal A, Tvrda E, Sharma R. Relationship amongst teratozoospermia, seminal oxidative stress and male infertility. Reprod Biol Endocrinol 2014;12:45.   DOI
37 Zhu DL, Zhang HG, Wang RX, Jiang YT, Liu RZ. Re-evaluation of the value of sperm morphology in classical in vitro fertilization in a Northeastern Chinese population. J Int Med Res 2019;47:4134-42.   DOI