• International Journal of Oral Biology
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• 제30권1호
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• pp.31-37
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• 2005

Treponema maltophilum, a Group IV oral spirochete, is associated with periodontitis and endodontic infections. In this study we analyzed the functional role of the major surface protein of this organism (MspA) in human gingival fibroblasts (HGFs). The full-length gene encoding MspA was cloned and expressed in Escherichia coli by using the expression vector pQE-30. The recombinant protein (rMspA) was purified by affinity chromatography with nickel-nitrilotriacetic acid agarose and possible contamination of E. coli endotoxin in rMspA was removed by using polymyxin B-agarose. rMspA significantly induced the expression of pro inflammatory cytokines like IL-6 and IL-8 and intercellular adhesion molecule (ICAM)-1 in HGFs, when analyzed by reverse transcription-PCR, flow cytometry, and enzyme-linked immunosorbent assay. Our results indicate that MspA of T. maltophilum may play an important role in amplifying the local immune response by upregulating the expression of proinflammatory cytokines and ICAM-1.

• Journal of Ecology and Environment
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• 제31권4호
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• pp.255-260
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• 2008

A commonly held view in studies of character displacement is that character states of both species are shifted in areas of sympatry. This view has been confirmed in an overwhelming number of cases for ecological character displacement. Excluding species pairs in which one of the two interacting species is found only within the distribution of the other species and species displaying gynogenesis, the pattern of reproductive character displacement is asymmetrical in that the shift in character states between areas of symaptry and allopatry occurs in only one of the two interacting species. Hypotheses for the reasons behind this asymmetry in reproductive character displacement include (1) homogenization by gene flow, (2) other mechanisms of reproductive isolation, and (3) sufficient reproductive isolation being provided by one of the interacting species exhibiting a pattern of reproductive character displacement. Because reproductive isolation can be achieved by divergence at any point in a sequence of premating reproductive behaviors and postmating developments, it is necessary to understand the mechanisms of reproductive isolation of two interacting taxa in areas of sympatry and allopatry and to analyze the relative contributions of potential factors to reproductive isolation to disentangle hypotheses for the patterns of asymmetry.

• Nuclear Medicine and Molecular Imaging
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• 제43권3호
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• pp.207-214
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• 2009

Positron emission tomogrpahy (PET) represents the most advanced scintigraphic imaging technology. With the increase in availability of PET, the clinical use of PET has grown in medical fields. This can be employed for cardiovascular research as well as for clinical applications in patients with various cardiovascular disease. PET allows non-invasive functional assessment of myocardial perfusion, substrate metabolism and cardiac innervation and receptors as well as gene expression in vivo. PET is regarded as the gold standard for the detection of myocardial viability, and it is the only method available for the quantitative assessment of myocardial blood flow. This review focuses on the clinical applications of myocardial perfusion PET in coronary artery disease.

• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1071-1078
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• 2006

Three buffalo populations viz. Bhadawari, Tarai and local buffaloes of Kerala were genotyped using 24 heterologous polymorphic microsatellite loci. A total of 140 alleles were observed with an average observed heterozygosity of 0.63. All the loci were neutral and 18 out of the 24 loci were in Hardy Weinberg Equilibrium. The $F_{IS}$ values (estimate of inbreeding) for 16 loci in all the three populations were negative. This indicated lack of population structure in the three populations. The effective number of immigrants was 5.88 per generation between the Tarai and Bhadawari populations which was quite high suggesting substantial gene flow. The genetic distances revealed closeness between the Tarai and Bhadawari populations which was expected from geographical contiguity. The FST values were not significantly different from zero showing no population differentiation. The Correspondence Analysis based on the allelic frequency data clustered the majority of the Tarai and Bhadawari individuals as an admixture.

• International Journal of Advanced Culture Technology
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• 제5권1호
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• pp.26-31
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• 2017

In this study, Emotion-DNA is constructed in the same way asthat the human DNA constructs the human body. Emotion-DNA is copied and translated in the same way as that the human DNA copies and translates itself. We made an attempt to embody the mind by Emotion-DNA like the symbols "A, T, G, C, U" that make up the chromosome of the human body. This is a diagram of the flow of emotions that the human body operates by literary works. These schemes present new directions for the therapeutic analysis of literary works and for the creation of therapeutic literary works. In this study, we analyzed the nominal Emotion-syllables as a framework of the structuring of emotional DNA. As a result, through the structuring of the emotional DNA, it was judged that the therapeutic action of the human body, which is included in the Rated Sijo among the literary works, can be more concrete and powerful than the works of other genres.

• Biotechnology and Bioprocess Engineering:BBE
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• 제3권1호
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• pp.6-10
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• 1998

A plasmid vector containing two reporter genes, mer-lux and lac-GFP, was transformed to both Escherichia coli and Pseudomonas putida. Their cellular activities and biofilm characteristics were investigated in flow-cell units by measuring bioluminescent lights and fluorescent levels of GFP. Bioluminescence was effective to monitor temporal cell activities, whereas fluorescent level of GFP was useful to indicate the overall cell activities during biofilm development. The light production rates of E. coli and P. putida cultures were dependent upon concentrations of HgCl2. Mercury molecules entrapped in P. putida biofilms were hardly washed out in comparison with those in E. coli biofilms, indicating that P. putida biofilms may have higher affinity to mercury molecules than E. coli biofilms. It was observed that P. putida expressed GFP cDNA in biofilms but not in liquid cultures. This may indicate that the genetic mechanisms of P. putida were favorably altered in biofilm conditions to make a foreign gene expression possible.

• Journal of information and communication convergence engineering
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• 제3권1호
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• pp.49-55
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• 2005

This study is to suggest a visualization technique to display the relations of associated data in an optimal way when trying to display the whole data on a limited space by dealing with a large amount of data with linked information. For example, if you track an IP address through several steps and display the data on a screen, or if you visualize the human gene information on a 3-dimensional space, then it becomes even easier to understand the data flow in such cases. In order to simulate the technique given in this study, the given algorithm was applied to a large number of nodes made in a random fashion to optimize the data and we visually observed the result. According to the result, the technique given in this study is more efficient than any previous method in terms of visualization and utilizing space and allows to more easily understand the whole structure of a node because it consists of sub-groups.

• 디지털산업정보학회논문지
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• 제15권2호
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• pp.19-28
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• 2019

The purpose of this study is to examine the trends on machine learning and deep learning research in the published journals from the Web of Science Database. To achieve the study purpose, we used the abstracts of 20,664 articles published between 1990 and 2017, which include the word 'machine learning', 'deep learning', and 'artificial neural network' in their titles. Twenty major research topics were identified from topic modeling analysis and they were inclusive of classification accuracy, machine learning, optimization problem, time series model, temperature flow, engine variable, neuron layer, spectrum sample, image feature, strength property, extreme machine learning, control system, energy power, cancer patient, descriptor compound, fault diagnosis, soil map, concentration removal, protein gene, and job problem. The analysis of the time-series linear regression showed that all identified topics in machine learning research were 'hot' ones.

• Neonatal Medicine
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• 제25권4호
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• pp.191-195
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• 2018

Leukocyte adhesion deficiency is a rare primary immunodeficiency and autosomal recessive disorder caused by a mutation in the gene encoding CD18, which is a constituent of leukocyte integrins. Clinical features usually begin with a delay in the separation of the umbilical cord in the neonatal period, and are characterized by marked leukocytosis with infection, delayed wound healing, and repeated bacterial and fungal infections. We experienced a case of leukocyte adhesion deficiency diagnosed in the neonatal period, in which a late preterm infant admitted to neonatal intensive care unit presented with a septic hip. Flow cytometry analysis of whole blood showed a decrease in the expression of CD11b/CD18. This is the first case of leukocyte adhesion deficiency with neonatal septic hip diagnosed in Korea.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 추계학술대회
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• pp.132-132
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• 2020

아직까지 국내에서 GM작물이 상업화를 위해 승인된 예는 없지만 생명공학기술의 발전으로 GM작물의 개발은 급속한 증가 추세에 있다. 비의도적인 방출로 인해 미승인 LMO 목화가 전국적으로 재배되어 국립종자원 주관으로 양성 판정된 재배지의 목화를 폐기 처분하였으나(2017), GM작물이 유해하다는 인식과 환경에 방출되어 생태계를 교란시킨다는 인식이 팽배해 있는 현실에서 과학적으로 유전자의 이동성을 검증하는 노력이 중요하다. 자식성 작물의 화분의 이동성 조사를 위해 중앙의 코어 위치에 LM작물을 식재한 후 LM작물 주변에 재배품종을 심어 유전자이동 가능성을 조사하고 재배 환경에 의한 영향을 평가하기 위해 포장 주변 기상상황 데이터-온도, 습도, 풍속, 풍향, 기압, 강수량 등을 분석하고 기상상황이 화분의 전이에 미치는 영향 조사하였다.