• Title/Summary/Keyword: Gem

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Synthesis and characterization of LiCoO2 thin film by sol-gel process (Sol-gel법에 의한 LiCoO2 박막의 합성과 특성평가)

  • Roh, Tae-Ho;Yon, Seog-Joo;Ko, Tae-Seog
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.24 no.3
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    • pp.94-98
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    • 2014
  • $LiCoO_2$ thin film has received diverse attention as cathodes material of thin-film micro-batteries. In this study, $LiCoO_2$ thin films were synthesized on Au substrates by sol-gel spin coating method and an annealing process. Their structures were studied using X-ray diffraction and Raman Spectroscopy. The particle morphologies of these thin films were observed by Scaning electron microscope. From the results of X-ray diffractometry and Raman Spectroscopy analyses, it was found that as-grown films had the structure of spinel (LT-$LiCoO_2$) and layered-Rock-salt (HT-$LiCoO_2$) at $550^{\circ}C$ and $750^{\circ}C$ respectively. The annealed films at $650^{\circ}C$ were presumed to be the mixed state of these two types. Throlugh the scanning electron microscope, It was estimated that the particle size in as-grown films at $750^{\circ}C$, were larger crystilline particle than in those at the other lower temperature and well distributed in the film.

Molecular Cloning and Expression of Human Poly (ADP-ribose) Synthetase cDNA in E. Coli (인간 Poly(ADP-ribose) Synthetase cDNA의 클로닝 및 대장균에서의 발현)

  • 이성용;김완주;이태성;박상대;이정섭;박종군
    • The Korean Journal of Zoology
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    • v.39 no.3
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    • pp.248-256
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    • 1996
  • The present study was performed to clone and express human poly (ADP-ribose) synthetase (PARS) cDNA in E coli. For these purposes, the CDNA for human poly (ADP-ribose) synthetase, encoding the entire protein, was cloned into pGEM-7Zf(+). The resulting recombinant plasmid pPARS6.1 was restriction enzyme mapped and its identity was confirmed by Southern blot analysis. The pPARS6. 1 contained full-length CDNA of human PARS and the nudeotide sequences were identical with those reported previously. The recombinant protein which migrated as a unique 120 kDa band on 10% SDS-polyacrylamide gels, was identified as PARS by Southwestern blots using nick-translated DNA probes and by activity gels and activity blots using 32 P-NAD as a substrate for poly (ADP-ribose) synthetase (PARS). The signals corresponding to 120 and 98 kDa proteins were obtained following IPTG (0.4 mM) induction of the PARS cDNA cloned into Xba I-digested pGEM-7Zf(+) vector. Nonspecific signals corresponding to 45 and 38 kDa proteins were also shown in both IPTG-induced and noninduced cells. The nonspecific proteins may be products of incomplete translation or proteolytic products of intact PARS.

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Alcaligenes eutrophus 균주의 성장과 Poly-Beta-hydroxybutyrate 합성에 미치는 포도당과 암모늄농도의 영향

  • 이용우;유영제
    • Microbiology and Biotechnology Letters
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    • v.18 no.6
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    • pp.607-612
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    • 1990
  • The biodegradation of Aroclor 1242 was investigated by the mixed cultivation of the natural bacterial isolates and a genetically engineered microorganism (GEM). The natural strain of MS-1003 degraded the Aroclor 1242 through the ortho-cleavage pathway, while the other strains through the meta-cleavage pathway. When the MS-1003 strain was additionally inoculated into the 1 day culture of the DJ-26 strain and then cultivated for 2 days, the Aroclor was degraded up to 86% and resulted in increase of the meta-cleavage product. But in the MS-1003 culture inoculated with the DJ-26, degradation of the Aroclor was limited to the level of each pure culture. By the mixed cultivation of the DJ-26 strain together with the DJ-12 or its GEM strain of DF-10, which degrades the Aroclor through the meta-cleavage pathway, degradation of the Aroclor as well as production of the meta-cleavage compound were lower than those of each pure culture. The degradation of Aroclor 1242 by the GEM strain was not improved over the parental strain. Therefore, a form of cometaboiism of Aroclor 1242 was found in the mixed culture of the DJ-26 and MS-1003 strains which degrade the Aroclor through the different metabolic pathway, but in the mixed culture of the DJ-26 and DJ-12 strains degrading Aroclor 1242 through the same pathway, a kind of competetion for the substrate was observed.

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Development of Colored Gemstone Products Through Analysis of Color Preference (색채 선호 분석을 통한 유색보석 제품 개발)

  • Lee, Ki-Sang
    • The Journal of the Korea Contents Association
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    • v.13 no.6
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    • pp.116-124
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    • 2013
  • This study aims to develop colored gemstone products based on the analysis of color preference. Colored gem stones were classified by their colors using Munsell's ten basic colors and the color preference of women consumers in their twenties was analyzed, in consideration of trend analysis and design image description analysis, which led to the direction of product development. The analysis of color preference seems to suggest that consumers preferred blue quartz, cahnite, spessartite garnet, the colors of which are rather less known to ordinary consumers, to the other more common kinds of gem stones. Through the analysis of the trends and adjectives to describe preferable design images, the current study has made it possible to explore the designs for the future development of colored jewelry products, which would satisfy the consumption value and individual tastes of the consumers. It is expected that the current study may contribute to the jewelry industries and designers as a basic data for developing colored gem stone products.

A Systems Engineering Approach to FEED Work Process Development for Refinery Plant (시스템 엔지니어링 접근 방법에 의한 정유 플랜트의 FEED 수행 업무 프로세스 개발)

  • Kim, Sun Young;Cha, Jae-Min;Kim, Junpil;Suh, Suk-Hwan;Sur, Hwal Won
    • Journal of the Korean Society of Systems Engineering
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    • v.10 no.1
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    • pp.1-15
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    • 2014
  • Refinery plant producing petroleum products from crude oil has significantly contributed to the creation of the national interests as a leading engineering industries. However, domestic Engineering Procurement Construction (EPC) companies are facing heavy competition for orders. Domestic EPC companies as EPC contractors are faced with some problems such as undertaking responsibility for FEED packages produced by other FEED companies. But domestic EPC contractors are unfamiliar to development and validation of FEED packages. It causes poor profitability and lower competitiveness of domestic companies. It is necessary for domestic companies to have capability to perform FEED activities in order to overcome these limitations instead of focusing on EPC phase after FEED phase. The systematic procedure is needed to perform the FEED activities, however, there are present difficulties on it due to the lack of experience in FEED packages development which require various engineering knowledge of chemical process, mechanics, electrics, instrumentation, civil engineering. This study has applied systems engineering method which is multi-disciplinary approach to derive and verify the solution to meet the customer's needs when the complex system is developed to task execution process development of FEED activities for refinery plant. The problems that may occur in the future were identified in advance by taking into account the various stakeholders and system context through the application of systems engineering. It helps to develop the task execution process systematically. The developed task execution process of FEED activities is planned to make effectiveness verified by engineering professionals experienced in FEED and continually enhance this process by field application.

A Systems Engineering Approach to Designing Continuous Casting System in Iron and Steel Making Plant (시스템 엔지니어링 방식에 의한 철강 연속 주조 시스템 설계)

  • Shin, Kee-Young;Hong, Dae Geun;Yoon, Soo Cheol;Suh, Suk-Hwan
    • Journal of the Korean Society of Systems Engineering
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    • v.10 no.2
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    • pp.21-31
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    • 2014
  • Recently, global market competition of iron and steel products is ever increasing due to over-supply from increased number of industries in rapidly growing countries, such as China, Brazil, and Indonesia. To occupy the big market, major industries are trying to develop high quality, high performance steel products via developing a new iron and steel making process. In other words, development of a new and innovative steel plant is a key to cope with the tough situation. Design and development for the life cycle of iron and steel making plant is very much complex and multi-disciplinary. In this paper, Plant Systems Engineering (PSE), a tailored SE process for industrial plant based on ISO/IEC 15288 is used for the design of Continuous Casting Process (CCP) Plant system. The CCP is a crucial process in steel making plant, whose design technology is occupied by the advanced foreign companies. For the sake of increasing engineering capability for the design of CCP, we applied PSE Process for the renovation of the existing CCP Process. Through the study, we were convinced that the applied method can be used for other plant systems, and SE is really the way of thinking, design, and development of modern complex and multi-disciplinary systems where high risk factors are present throughout the whole life cycle.