• Title/Summary/Keyword: Gel-dry

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Effect of Disodium Fumarate on In vitro Rumen Fermentation of Different Substrates and Rumen Bacterial Communities as Revealed by Denaturing Gradient Gel Electrophoresis Analysis of 16S Ribosomal DNA

  • Mao, S.Y.;Zhang, G.;Zhu, W.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.4
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    • pp.543-549
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    • 2007
  • Two experiments were conducted to investigate the effects of disodium fumarate on the in vitro rumen fermentation profiles of different substrates and microbial communities. In experiment 1, nine diets (high-forage diet (forage:concentrate, e.g. F:C = 7:3, DM basis), medium-forage diet (F:C = 5:5, DM basis), low-forage diet(F:C = 1:9, DM basis), cracked corn, cracked wheat, soluble starch, tall elata (Festuca elata), perennial ryegrass and rice straw) were fermented in vitro by rumen microorganisms from local goats. The results showed that during 24 h incubations, for all substrates, disodium fumarate increased (p<0.05) the gas production, and tended to increase (p<0.10) the acetate, propionate and total VFA concentration and decrease the ratio of acetate to propionate, whereas no treatment effect was observed for the lactate concentration. The apparent DM loss for tall elata, perennial ryegrass and rice straw increased (p<0.05) with the addition of disodium fumarate. With the exception of tall elata, perennial ryegrass and rice straw, disodium fumarate addition increased the final pH (p<0.05) for all substrates. In experiment 2, three substrates (a high-forage diet, a medium-forage diet and a high concentrate diet) were fermented by mixed rumen microbes in vitro. A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was applied to compare microbial DNA fingerprints between substrates at the end of 24 h incubation. The results showed that when Festuca elata was used as substrate, the control and disodium fumarate treatments had similar DGGE profiles, with their similarities higher than 96%. As the ratio of concentrate increased, however, the similarities in DGGE profiles decreased between the control and disodium fumarate treatment. Overall, these results suggest that disodium fumarate is effective in increasing the pH and gas production for the diets differing in forage: concentrate ratio, grain cereals and soluble starch, and in increasing dry matter loss for the forages (tall elata, perennial ryegrass and rice straw) in vitro, whereas its effect on changes of ruminal microbial community may largely depend on the general nature of the substrate.

Effect of Drying Methods on Physicochemical Properties of Agar (건조 방법이 한천의 물리${\cdot}$화학적 특성에 미치는 영향)

  • KIM Oc-Do;KIM Yuck-Yong;LEE Nahm-Gull;CHO Young-Je;LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.5
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    • pp.681-688
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    • 1996
  • To investigate the effort of drying methods on the physicochemical properties of agar, gel strength, viscosity, melting and setting point, and phase transition by differential scanning calorimetery (DSC) during its heating were determined. In addition the structural differences of agar powder by scanning electron microscope (SEM) was examined. The most shortest onset temperature of gel strength increase was extruding method among any other methods. Viscosity of agar with hot air method, 400.00 cps at $45^{\circ}C$, was markedly increased, but with spraying and extruding ones were little change. The melting and setting point, and the temperature for maximum endothermic and enthalpy for agar with extruding one, $80.01^{\circ}C,\;36.05^{\circ}C\;and\;61.72^{\circ}C,\;0.73\;cal/g$, respectively, were lowest among the drying ones. But in the case of reheating after gelling, there were little change in all methods. Observing the surface structure of agar with SEM, extruding method showed the most unstable with absorptive property.

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Printing Performance Evaluation of Water-dispersed Pigment Ink according to Additive Conditions of Film Substrate Surface Coating Agent (필름기재 표면 코팅제의 첨가물질 조성 조건에 따른 수분산 안료잉크의 프린팅 성능 평가)

  • Hyeok-Jin Kim;Hye-Ji Seo;Eun-Ha Kang;Min-Woo Han;Dong-Hyeon Lee;Dong-Jun Kwon;Jin-Pyo Hong
    • Textile Coloration and Finishing
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    • v.35 no.4
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    • pp.196-205
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    • 2023
  • Water-dispersed pigment is on-going study for without air pollution in the textile and print industry. Primer treatment is essential for the substrate to improve the printing quality of eco-friendly water-dispersed pigment ink. Otherwise in the case of untreated primer, the water-dispersed pigment ink will dry onto the surface and cause defective images. This study was conducted on film substrate coating (primer) to fix eco-friendly water-dispersed pigment ink on film substrate. The drying, bleeding, and color strength of the pigment ink were examined depending on the composition and mixing ratio of the coating solution. The mixing ratio of silica gel in the coating film is increased to 0, 0.5, 1, 2 and 3 and results that DK-1-3 of silica gel ratio of 1 showed the lowest bleeding such as 52%, the letter thickness of 0.76mm and DK-1-5 of SG ratio of 3 showed the highest bleeding such as 304%, the letter thickness of 2.02mm. The mixing ratio of SPA in the coating film is increased to 2.5, 5, 7.5, SPA ratio of 7.5 has a bleeding ratio of 9% and letter thickness of 0.544mm. It showed the closest value to 0.5mm. According to the result, the optimal mixing ratio of binder, polymer coagulant, silica gel is 100:7.5:1.

Some Properties of Fresh and Ripened Traditional Akcakatik Cheese

  • Simsek, Bedia;Tuncer, Yasin
    • Food Science of Animal Resources
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    • v.38 no.1
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    • pp.110-122
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    • 2018
  • Akcakatik cheese (yogurt cheese) is produced by drying strained yogurt with or without adding cloves or black cumin. The main objective of this study was to detect the properties of both fresh and ripened Akcakatik cheeses and to compare them. For this purpose the biogenic amine content, volatile flavor compounds, protein degradation level, chemical properties and some microbiological properties of 15 Akcakatik cheese samples were investigated. Titratable acidity, total dry matter, NaCl, total nitrogen, water soluble nitrogen, ripened index, histamine, diacetyl and acetaldehyde levels were found to be higher in ripened cheese samples than in fresh cheese samples. On the other hand, the clove and black cumin ratios were found to be higher in the fresh cheese samples. Sodium dodecyl sulphate polyacrylamide gel electropherograms of cheese samples showed that protein degradation was higher in ripened cheese samples than in fresh samples, as expected. The dominant Lactic acid bacteria (LAB) flora of Akcakatik cheese samples were found to be Streptococcus salivarius subsp. thermophilus and Lactobacillus delbrueckii subsp. bulgaricus.

Dry Media Reaction Procedure for Synthesis of α,β-Unsaturated Acids, α-Cyanoacrylonitriles and α-Cyanoacrylates via Knoevenagel Condensation Using NaHSO4·SiO2 Catalyst (NaHSO4·SiO2 촉매를 이용한 Knoevenagel 축합을 거친α,β의 불포화산, α-Cyanoacrylonitriles와 α-Cyanoacrylates의 합성을 위한 무수조건하의 반응 절차)

  • Gopalakrishnan, M.;Sureshkumar, P.;Kanagarajan, V.;Thanusu, J.;Thirunavukkarasu, S.
    • Journal of the Korean Chemical Society
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    • v.51 no.4
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    • pp.346-351
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    • 2007
  • Efficient stereoselective synthesis of α,β-Unsaturated acids, α-Cyanoacrylonitriles and α-Cyanoacrylates has been carried out in the presence of NaHSO4·SiO2 under solvent?free conditions with an E-geometry.

Estimation of Bituminous Concrete Property Based on Molecular Size Distribution of Asphalt Cement (아스팔트 시멘트의 분자립도 분포특성에 따른 아스팔트의 성질분석)

  • 김광우;연규석
    • Magazine of the Korea Concrete Institute
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    • v.3 no.1
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    • pp.71-78
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    • 1991
  • Moleclar size distribution of three AC-20 asphalt cements were observed using high pressure - gel permeation chromatograph (HP-GPC). Bituminous concrete specimens were made using the asphalt cements and one aggre¬gate in laboratory to measure mechanical properties, tensile strength CIS) and resilient modulusCM,J. in dry and wet conditons. Results of mechanical property tests and profile of molecular size distribution of asphalt cements were analyzed to evaluate the relatonship of mechanical property with chromatogram. Regression analy¬sis showed a strong correlation between a mechanical property and sliced percent areas of chromatogram. Mathematical prediction models were developed based on the regression analysis. It was shown that characteris¬tics of asphalt cement chromatogram could be successfully used for estimation of selected mechanical property of asphalt concrete .

Physicochemical and structural properties of lintnerized starches from sweet Potato (고구마전분의 산처리 특성과 산분해 잔사의 구조적 특성)

  • Kim, Sung-Ran;Ahn, Seung-Yo
    • Applied Biological Chemistry
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    • v.35 no.3
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    • pp.196-201
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    • 1992
  • Characteristics on lintnerization of dry type (Suwon 147) and moist type (Hwangmi) sweet potato starches were investigated. Chain distribution of lintnerized starches was also studied by debranching with pullulanase. Hydrolytic patterns of two starches showed two distinct stages and hydrolysis extents of Suwon 147 starch were lower than those of Hwangmi starch. The relative crystallinities of Suwon 147 starches were higher than those of Hwangmi starch. The elution profiles of lintnerized starches were composed of two peaks about degree of polymerization (DP) 25 and DP 15. The elution profiles of debranched samples showed only one peak about DP 15 and peak DP of Suwon 147 lintnerized starch was higher than that of Hwangmi.

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Level of Heavy Metals in the Onsan Bay in Korea and Involvement of Metal Binding Protein in the Accumulation of Cadmium in Littorina brevicula

  • Paek, Soo-Min;Chung, Soohee;Lee, In-Sook
    • The Korean Journal of Ecology
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    • v.22 no.2
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    • pp.95-100
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    • 1999
  • The heavy metal concentrations in seawater and winkles (Littorina brevicula) collected from the Onsan bay area in southeast of Korea were analyzed. The heavy metal concentrations in the seawater obtained from the most polluted site showed approximately 189. 205. and 110 fold higher cadmium, copper. and zinc concentrations, respectively. than the uncontaminated control site. The contamination levels of these metals in winkles were 11.08 - 2.35, 334.5 - 212.5, and 426.0 - 499.2$\mu\textrm{g}$ per gram dry body weight. respectively. The concentrations of all three metals in both the seawater and winkles decreased gradually with increasing distance from Daejeong stream, suggesting the stream being the major source of heavy metal input into the bay. Among the four body parts of digestive gland and gonad. gill. kidney, and remaining tissue in contaminated winkles, kidney showed the highest accumulation level of cadmium: copper and zinc, however. were more or less distributed among the four body parts. Upon gel filtration chromatography of the cytosol from the kidney of cadmium induced winkles, one cadmium peak corresponded to the elution peak of horse kidney metallothionein.

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Quantitative Changes of Polyphenolic Compounds in Mulberry (Morus alba L.) Leaves in Relation to Varieties, Harvest Period, and Heat Processing

  • Lee, Won Jeong;Choi, Sang Won
    • Preventive Nutrition and Food Science
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    • v.17 no.4
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    • pp.280-285
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    • 2012
  • Six polyphenolic compounds, such as chlorogenic acid (CA), rutin (RT), isoquercitrin (IQT), quercetin-3-O-(6-O-malonyl)-${\beta}$-D-glucoside (QMG), astragalin (AG), kaempferol-3-O-(6-O-malonyl)-${\beta}$-D-glucoside (KMG), were isolated from mulberry leaves by a series of isolation procedures, such as Diaion HP-20, silica-gel, Sephadex LH-20, and ODS-A column chromatographies. The chemical structures of the phenolic compounds were identified by UV and NMR spectral analyses. Levels of polyphenols in mulberry leaves from six different mulberry cultivars ranged from 1,042.16 to 1,871.97 mg% per dry weight; Guksang cultivar showed the highest levels of polyphenols, whereas Gaeryangdaehwa contained the least polyphenol contents. Generally, levels of polyphenols in mulberry leaves decreased with increasing harvest time, except for Yoolmok, but increased with heat processing time, except QMG and KMG. These results suggest that the heat processed mulberry leaves of Guksang cultivar harvested in early May can be potentially useful sources for production of high quality mulberry leaf teas.

Screen-printed carbonaceous matrrials for photocapacitor electrode (스크린 프린터에 의한 광캐패시터용 카본 전극 제작)

  • Choi, Woo-Jin;Kwak, Dong-Joo;Sung, Youl-Moon;Ha, Soon-Ho
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2009.10a
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    • pp.411-414
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    • 2009
  • Photo-capacitor electrodes are attracting great attention because of their high capacitance and potential applications in electronic devices. Carbon capacitor, active carbon capacitor and its combination will be fabricated using simple sandwich capacitor electrode method as carbonaceous material on each type of capacitor electrodes with 20 ${\times}$ 15 mm cell size. Carbon/active carbon cell was fabricated using sol-gel process with 120oC dry temperature in l hour and using sintering process with 500oC in 2 hour. The effect of sintering temperature on carbon properties was also investigated with X-ray diffraction technique to get the best sintering temperature. The detail of fabrication process will be explained. Elemental composition in electrode material can be measured using quantitative spectroscopic as and a cyclic voltammetric technique was used to study the combined effects of electrode material and effect of annealing temperature and also time on the capacitance of thermally treated in capacitor electrode. In this work, characterization impedance technique is used to measurement of capacitance and giving complementary results. Active carbon as carbonaceous material has a better capacitance in charge/discharge process with mean thickness $32{\mu}m$ and with particle size $1{\mu}m$ to $4.5{\mu}m$ in 20 ${\times}$ 15 mm sample size of capacitor electrode.

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