• 한방재활의학과학회지
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• 제21권4호
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• pp.1-12
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• 2011

Objectives: This study was carried out in order to examine the effects of Gastrodiae rhizoma(GR) ethanol extract on neuronal apoptosis in intracerebral hemorrhage(ICH)-induced rats. Methods: ICH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type VII in Sprague-Dawley rats. GR was orally given once a day for 3 days after ICH. Histological changes of the peri-hematoma regions were observed by cresyl vioIet staining. Bcl-2-associated X protein(Bax), B-cell blastoma 2(BcI-2) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL) expressions in the affected regions were performed by immunohistochemistry. Results: 1. GR reduced apoptotic bodies and swelling neurons in the peri-hematoma regions of ICH-induced rats. 2. GR significantly reduced TUNEL positive cells in the peri-hematoma regions of ICH-induced rats. 3. GR significantly reduced Bax positive cells in the peri-hematoma regions of ICH-induced rats. 4. GR did not influence Bcl-2 expression in the peri-hematoma regions of ICH-induced rats. Conclusions: These results suggest that GR has neuroprotective effects against ICH-induced apoptosis.

• 대한한방내과학회지
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• 제39권4호
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• pp.717-731
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• 2018

Objectives: The purpose of this research is to investigate clinical studies of essential tremor in China. Methods: We investigated clinical studies of Traditional Chinese Medicine therapies in traditional Chinese medical journals for essential tremor through the China National Knowledge Infrastructure (CNKI) database. In total, 15 clinical articles were analyzed. This study examined the authors; published years; types of study; treatment methods for the treatment and control groups; periods; outcome assessment; and results. Results: Nine articles used acupuncture, 6 articles used herbal therapy. The most commonly used acupoints were GV20, GB20, LR3, LI4, GB34, SP6, and EX-HN1. The most commonly used herbs were Paeoniae Radix Alba, Angelicae Gigantis Radix, Cnidii Rhizoma, Gastrodiae Rhizome and Uncariae Ramulus et Uncus. Total effective rate was 66.67-96.7%; the treatment group values were significantly higher than the control group values in all papers. Tremor score, HAMD, curative effect of TCM syndrome, and incidence of adverse effects were significantly more affected in the treatment group than in the control group. Conclusion: More active clinical studies are needed on essential tremor, and the results of this study can be used as a basis for future research.

• Journal of Applied Biological Chemistry
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• 제59권3호
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• pp.179-188
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• 2016

본 연구의 목적은 증숙 및 발효과정을 거친 천마추출물이 LPS로 유도된 간 손상에 미치는 효능을 평가하고자 하였다. 증숙 횟수 및 발효균을 달리한 25가지 시료 중 DPPH와 ABTS 라디칼 소거능을 측정하여 생 천마(GR0F0)와 6회 증숙한 후, Saccharomyces cerevisiae균으로 발효한 GR6F4간의 비교실험이 가능할 것으로 사료되었다. 마우스는 6마리씩 4그룹(정상군, 대조군, GR0F0 투여군, GR6F4투여군)으로 나누어 실험을 진행하였다. LPS를 투여하기 전, 각 시료를 200 mg/kg/day로 8일간 경구 투여하였으며, 간 손상을 유발하기 위하여 LPS를 20 mg/kg로 복강투여하였다. 혈액에서 AST, ALT를 측정한 결과, 대조군에서 그 수치가 증가하였고, GR6F4군에서 감소하였을 뿐 아니라, 혈액 및 간에서 측정한 ROS 수치 또한 유의하게 감소하였다. 간조직에서 AP-1, COX-2, TNF-${\alpha}$와 INOS를 측정한 결과, GR0F0 또한 감소하였으나, 증숙 및 발효과정을 거친 GR6F4군에서 더욱 큰 효과를 보이는 것을 확인하였다. 이러한 결과들은 GR6F4의 뛰어난 항산화 및 항염증 효과를 증명하며, 그러므로 증숙 및 발효과정을 거친 천마는 간 손상에 보호효과가 있다고 보여진다.