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http://dx.doi.org/10.3839/jabc.2016.033

Protective effects of Gastrodia rhizoma and steamed & fermented Gastrodiae rhizoma with anti-oxidant efficacy and suppression of NFκB signaling pathway on LPS-induced liver injury  

Kim, Hyoung-Jin (College of Korean Medicine, Daejeon University)
Kwon, O Jun (Gyeongbuk Regional industry Evaluation, Daegyeong Institute for Regional Program Evalution)
Lee, Ah Reum (College of Korean Medicine, Daegu Haany University)
Roh, Seong-Soo (College of Korean Medicine, Daegu Haany University)
Seo, Young-Bae (College of Korean Medicine, Daejeon University)
Publication Information
Journal of Applied Biological Chemistry / v.59, no.3, 2016 , pp. 179-188 More about this Journal
Abstract
This study is aimed to evaluate the protective effect of Gastrodiae rhizoma and steamed, dried & fermented Gastrodiae rhizoma on Lipopolysaccharide (LPS)-induced hepatic injury in the mice model. Sample was selected to GR0F0 (not processed gastrodia rhizome) and GR6F4 (fermented with Saccharomyces cerevisiae before steamed and dried 6 times) based on 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid, and High-performance liquid chromatography analysis. Mice were randomly divided into 4 groups - Normal group, vehicle group (LPS treated), GR0F0 group (fed GR0F0 before LPS treated) and GR6F4 group (fed GR6F4 before LPS treated) with 6 mice in each group. GR0F0 group and GR6F4 group were fed each extract 200 mg/kg/day during 8 days. LPS 20 mg/kg injected to the experimental groups as abdominal injection. We measured aspartate aminotransferase, alanine amino-transferase in serum. GR0F0 and GR6F4 showed a significant decrease compared to the vehicle group. As a result of measuring the ROS, GR6F4 group showed a significant reduction in both the serum and liver tissues compared to the vehicle group. GR0F0 group showed a significant reduction only in the liver tissues. Activator protein-1, cyclooxygenase-2, and Inducible nitric oxide synthase were significantly decreased GR0F0 group and GR6F4 group. But tumor necrosis factor alpha only showed a significant reduction in GR6F4 group. GR0F0 and GR6F4 groups against liver damage in mice with LPS. That showed significant effects on anti-oxidant and anti-inflammatory action. The effects of GR6F4 group showed superior results compared to GR0F0 group. Therefore, Steamed, dried & fermented Gastrodia rhizoma was might have a protective effect on liver injury.
Keywords
Anti-inflammation; Anti-oxidation; Fermentation; Gastrodiae rhizoma; Lipopolysaccharide; Liver injury;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Abe J, Kusubara M, Ulevitch RJ, Berk BC, Lee JD (1996) Big mitogenactivated protein kinase 1 (BMK1) is a redox-sensitive kinase. J boil Chem 271: 16586-16590   DOI
2 Baud V, Karin M (2009) Is NF-${\kappa}B$ a good target for cancer therapy? Hopes and pitfalls. Nat Rev Drug Discov 8: 33-40   DOI
3 Briscoe DM, Cotran RS, Pober JS (1992) Effect of tumor necrosis factor, lipopolysaccharide, and IL-4 on the expression of vascular cell adhesion molecule-1 in vivo: correlation with CD3+ T cell infiltration. J Immunol 149: 2594-2960
4 Choi JH (2012) The Study on Chemical Analysis and Increased Physiological Activities of Fermented Gastrodia Extracts (Gastrodia elata var. Blume). Dissertation, Yeungnam University
5 Choi SR, Jang I, Kim CS, You DH, Kim JY, Kim YG, Ahn YS, Kim JM, Kim YS, Seo KW (2011) Changes of Components and Quality in Gastrodiae Rhizoma by Different Dry Methods. Korean J Medicinal Crop Sci 19: 354-361   DOI
6 Crofford LJ (1997) COX-1 and COX-2 tissue expression: implications and prediction. J Rheumatol Suppl 49: 15-19
7 Gabriel LP, William R, Hewitt (1982) Principles and Methods of toxicology. Raven Press. San Diego
8 Gamber JR, Harlan JM, Klebanoff SJ, Vadas MA (1985) Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor. Proc Natl Acad Sci USA 82: 8567-8571   DOI
9 Hayden MS, Ghosh S (2008) Shared principles in NF-${\kappa}B$ signaling. Cell 132: 344-362   DOI
10 Heo JC, Park JY, An SM, Lee JM, Yun CY, Shin HM, Kwon TK, Lee SH (2006) Anti-oxidant and Anti-tumor Activities of Crude extracts by Gatrodia elasta Blume. Korean J Food Preserv 13: 83-87
11 Kim GY, Song HJ (2002) Hanyak pojehak. Shinilbooks. Seoul
12 Kim JM, Moon YS, Yoon KY, Suh SG (2010) Quality Properties and Preference of Fermented Gastrodia elata Blume. Kor J Hort Sci Technol 28: 507-514
13 Ko CH (2010) Characterization of unique proteins from fresh and fermented Gastrodia elata. Dissertation, Yeungnam University
14 Kwon SU (2012) Anti-diabetic Effect of Fermented Gastrodia elata blume on STZ-induced Type 1 Diabetic Mice. Dissertation, Wonkwang University
15 Lee KH, Rhee KH, Kim BS, Choi YH, Kim CH (2013) Sleep Inducing Effect of Gastrodia elata Fermented with Lactic Acid Bacteria. Kor J Pharmacogn 44: 281-285
16 Nathan C (1992) Nitric oxide as a secretory product of mammalian cells. FASEB J 6: 3051-3064   DOI
17 Sin MG (2008) Clinical Traditional Herbalogy. Yeongrimsa, Seoul
18 Vane JR, Mitchell JA, Appleton I, Tomlinson A, Bishop-bailey D, Croxtall J, Willoughby DA (1994) Inducible isoforms of cyclooxygenase and nitricoxide synthase in inflammation. National Acad Sciences 91: 2046-2050   DOI
19 Song MH, Bae JT, Ko HJ, Jang YM, Lee JD, Lee GS, Pyo HB (2011) Anti-Oxidant Effect and Anti-Inflammatory of Fermented Citrus unshiu Peel Extract by using Schizophyllum commune. J Soc Cosmet Scientists Korea 37: 351-356