• Analytical Science and Technology
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• v.16 no.1
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• pp.82-89
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• 2003

Isotope dilution mass spectrometry (IDMS) was applied to the determination of Ni, Cr, Mo in low alloy steel reference materials. The Mo isotope ratio measurement was performed by dynamic reaction cell inductively coupled plasma mass spectrometry (DRC-ICP/MS) using ammonia as a reaction cell gas. In the case of Ni and Cr measurement, all data were obtained at medium resolution mode (m/${\Delta}m=3000$) of double focusing sector field high resolution inductively coupled plasma mass spectrometry (HR-ICP/MS). For the method validation of the technique was assessed using the certified reference materials such as NIST SRM 361, NIST SRM 362, NIST SRM 363, NIST SRM 364, NIST SRM 36b. This method was applied to the determination of Ni, Cr and Mo in low alloy steel sample (CCQM-P25) provided by NMIJ for international comparison study.

• Food Science and Biotechnology
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• v.15 no.4
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• pp.572-577
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• 2006

The physiochemical interactions of 1-methylcyclopropene (1-MCP) and adsorbing agents can be described using a very powerful tool, inverse gas chromatography (IGC). Sorption behavior of 1-MCP on various adsorbing agents was assessed using the profile peaks of 1-MCP at an infinite dilution concentration using the IGC technique. Chromatogram peaks of 1-MCP adsorption were not observed for the adsorbing agent activated carbon. The forms of sorption isotherms followed Henry's law, and behaved according to the binding site theory. Specific retention volume and distribution coefficients for 1-MCP on the adsorbing agents were determined at 50, 60, 70, and $80^{\circ}C$, respectively. Silica gel had a much higher number of binding sites for 1-MCP compared to Tenax-TA and activated clay agents. Meanwhile, activated carbon proved to be a very strong binding agent for 1-MCP based on 1-MCP efficiency experiments on the selected adsorbing agents. However, as a proper means of delivering 1-MCP molecules to fresh food products, activated carbon is not fit for the binding and release of 1-MCP gas under dry or high humidity conditions because activated carbon has a strong affinity for 1-MCP, even when treated with distilled water.

• Journal of Advanced Marine Engineering and Technology
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• v.34 no.4
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• pp.485-490
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• 2010

MILD (Moderate and Intense Low Oxygen Dilution) combustion is a technique which is able to reduce NOx formation and to uniform temperature distribution in the furnace by recirculating the exhaust gas to the fresh air and fuel. This study focuses on finding optimal condition of MILD combustor by changing equivalence ratio with fuel and air flow. The present experiment employs six thermocouple sensors in the furnace, and two concentration probes of NOx and CO at the exhaust exit pipe respectively. The MILD combustion phenomena have been observed at the condition of equivalent ratios of 0.71~0.73, and the temperature uniformity, NOx and CO concentration are also examined at the MILD combustion condition.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.526-532
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• 2006

The aim of this study was to evaluate the chemical composition, in vitro DM degradability, ME and OMD of alfalfa-maize silage mixtures in comparison to pure maize and alfalfa silages, and to test the existence of associative effects of ensiling alfalfa forage with whole-crop maize using the in vitro gas production technique. Ensiling alfalfa with whole-crop maize had a significant (p<0.001) effect on chemical composition, pH, in vitro DM degradability, OMD and estimated ME values of mixtures. DM content of the resultant silages significantly increased with increasing proportion of whole-crop maize in the mixtures, whereas the pH value, crude protein (CP), acid detergent fibre (ADF) and ash contents of mixtures decreased due to the dilution effect of whole-crop maize which was low in CP, ADF and ash. The pH values of all alfalfa-maize silage mixtures were at the desired level for quality silage. Gas production of alfalfa-maize silage mixtures at all incubation times except 12 h increased with increasing proportion of whole-crop maize. When alfalfa was mixed with whole-crop maize in the ratio 40:60, ME and OMD values were significantly (p<0.001) higher than other silages. Maximum gas production ($A_{gas}$) ranged from 65.7 to 78.1 with alfalfa silage showing the lowest maximum gas production. The results obtained in this study clearly showed that maximum gas production increased with increased percentage of whole-crop maize in the silage mixtures (r = 0.940, p<0.001). It was concluded that ensiling alfalfa with whole-crop maize improved the pH, OMD and ME values. However, trials with animals are required to see how these differences in silage mixtures affect animal performance.

• Analytical Science and Technology
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• v.19 no.6
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• pp.553-558
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• 2006

In this study, an attempt was made to measure uncertainties involved in the VOC analysis for the VOC working standards prepared by a dilution technique using Tedlar bags. For this purpose, VOC standard gases of benzene, toluene, xylene, and styrene were prepared at four different concentrations (4, 8, 20, and 40 ppb). These standard samples were then loaded on to the GC system equipped with air server/thermal desorption (AS/TD) system. Each of these four standard concentrations was analyzed individually to derive their respective calibration results. These calibration data sets were then compared across four different compounds. According to this comparison, differences in calibration patterns were moderately insignificant within the selected concentration range of 4~40 ppb. It was also observed that the loss of styrene standard was fairly high compared to other VOCs investigated simultaneously. The results of our study suggest that the analytical uncertainty associated with the preparation of VOC starndard gas using a dilution technique can be assessed in a fairly reasonable manner for samples with a narrow concentration range.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.1
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• pp.144-152
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• 2006

Split injection has been known to reduce total hydrocarbon (THC) emission level and increase engine performance under certain operating conditions 1, 2). Exhaust Gas Recirculation (EGR) is a common technique adopted for nirtric oxides (NOx) reduction by the dilution of intake air, despite a sacrifice of simultaneous increase in THC and decrease in engine performance3). Thus, using split injection with adequate EGR may improve the emission level of UBHC, NOx and the engine performance compared to that of single-injection with or without EGR cases. The purpose of this study is to investigate the engine performance and emission levels at various engine operating conditions and injection methods when it is applied with EGR. The characteristics of single-injection and split-injection were investigated with various engine loads and EGR rates. The engine speed is changed from 800rpm to 1200rpm to investigate how the combustion characteristics are changing with increasing engine speed.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.5
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• pp.58-64
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• 2006

Homogeneous charge compression ignition(HCCI) combustion is an advanced technique for reducing the hazardous nitrogen oxide(NOx) and particulate matter(PM) in a diesel engine. NOx could be reduced by achieving lean homogeneous mixture resulting in combustion temperature. PM could be also reduced by eliminating fuel-rich zones which exist in conventional diesel combustion. However previous researches have reported that power-output of HCCI engine is limited by the high intensive knock and misfiring. In an attempt to extend the upper load limit for HCCI operation, supercharging in combination with Exhaust Gas Recirculation(EGR) has been applied: supercharging to increase the power density and EGR to control the combustion phase. The test was performed in a single cylinder engine operated at 1200 rpm. Boost pressures of 1.1 and 1.2 bar were applied. High EGR rates up to 45% were supplied. Most of fuel was injected at early timing to make homogeneous mixture. Small amount of fuel injection was followed near TDC to assist ignition. Results showed increasing boost pressure resulted in much higher power-output. Optimal EGR rate influenced by longer ignition delay and charge dilution simultaneously was observed.

• Mass Spectrometry Letters
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• v.3 no.1
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• pp.21-24
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• 2012

$5{\alpha}$-Dihydrotestosterone (DHT) is the primary active metabolite of testosterone, catalyzed by $5{\alpha}$-reductase ($5{\alpha}R$) in the skin, prostate, and liver. In this study, the $5{\alpha}R$ activity in rat liver S9 fraction in the presence of a NADPH-generating system was evaluated and compared by gas chromatography-mass spectrometry (GC-MS)-based in vitro assays. Testosterone and a $5{\alpha}R$ inhibitor, finasteride, were added to the S9 fractions and incubated at $37^{\circ}C$ for 1 h. Both testosterone and DHT were quantitatively measured and compared with two different GC-MS-based steroid profiling techniques. DHT was not detected by conventional GC-MS analysis in the absence of finasteride when the concentration of testosterone in the S9 fraction was less than $0.2{\mu}M$, whereas the isotope-dilution GC-MS (GC-IDMS) system was able to evaluate the $5{\alpha}R$ activity. Because the S9 fraction contains more reactive enzymes and is easier to collect from tissues compared with a microsomal solution, the combination of the S9 fraction and GC-IDMS technique may be a promising assay for evaluating the $5{\alpha}R$ activity in large-scale clinical studies.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3817-3824
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• 2013

Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP- and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with $^{78}Se$ spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL $min^{-1}$ Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was $22.8{\pm}3.4\;ng\;g^{-1}$, $45.2{\pm}1.7\;ng\;g^{-1}$, and $16.1{\pm}2.2\;ng\;g^{-1}$, respectively when $^{80}Se/^{78}Se$ was used. The sum of these selenoproteins ($84.1{\pm}4.4\;ng\;g^{-1}$) agrees well with the total selenium concentration measured with the ID method of $87.0{\pm}3.0\;ng\;g^{-1}$. Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

• Applied Biological Chemistry
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• v.37 no.4
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• pp.277-286
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• 1994

The pot experiment using $^{15}N$ isotope dilution technique was carried out to calculate the balance of nitrogen of surface applied urea in the rice-soil system. The $^{15}N$ concentration was determined by stable isotope ratio mass spcetrometer (model: VG ISO-GAS MM622). In the pots with $^{15}N$ labeled urea application at the rates of 15 and 30 kg N/10a, the percentage of nitrogen derived from fertilizer (NDFF) in rice was higher at the rate of 30 kg N/10a (average 89%) than at the rate of 15 kg N/10a (average 64%). However, the recovery as percentage of fertilizer N by rice was higher at the rate of 15 kg N/10a (65.5%) than at the rate of 30 kg N/10a (54.2%). The percentage of the fertilizer N remained in extractable inorganic N form at the rates of 15 and 30 kg N/10a were $13.5%\;(NH_4-N\;5.53%,\;NO_3-N\;7.99%)$ and $16.5%\;(NH_4-N\;7.49%,\;NO_3-N\;8.98%)$ in unplanted soil, and $2.0%\;(NH_4-N\;0.63%,\;NO_3-N\;1.32%)$ and$2.3%\;(NH_4-N\;0.87%,\;NO_3-N\;1.40%)$ in soil planted to rice, respectively. The dominant form of inorganic-N in soil after harvest was $NO_3-N$ form rather than $NH_4-N$ form regardless of urea application rate or rice cultivation. The percentage of the fertilizer N remained in organic N form at the rates of 15 and 30 kg N/10a were 65.0 and 41.8% in unplanted soil, and 23.7 and 26.9% in soil planted to rice, respectively. In conclusion, the efficiency of surface-applied urea was greater at the rate 15 kg N/10a than at the rate of 30 kg N/10a.