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Studies of separation and quantitation for selenium species in food (식품중의 셀레늄 화학종의 분리 및 정량연구)

  • Jang, Hee-Young;Min, Hyungsik;Lee, Jonghae;Pak, Yong-Nam
    • Analytical Science and Technology
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    • v.26 no.3
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    • pp.182-189
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    • 2013
  • The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. $C_8$ column ($Symmetryshield^{TM}\;RP_8$, 3.5 ${\mu}m$, $4.6{\times}150$ mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL $min^{-1}$). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few ${\mu}g$ $g^{-1}$ to few tens of ${\mu}g$ $g^{-1}$.

Effect of Fermentation Temperature and Salt Concentration on the Fermentation Rate of Dongchimi (발효온도와 소금농도가 동치미 발효속도에 미치는 영향)

  • Chang, Sang-Keun;Ko, Soon-Nam;Choi, Hee-Sook;Kim, Jong-Goon;Kim, Woo-Jung
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.398-402
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    • 1996
  • This study was carried out to compare the fermentation rates of Dongchimi as affected by fermentation temperature and salt concentration of brining solution. The Dongchimi was prepared by fermentation of the radish in salt solutions of 2.0, 3.2 and 5.1% NaCl respectively with addition of garlic, green onion, ginger at $4^{\circ}C,\;10^{\circ}C,\;20^{\circ}C$. The fermentation was classified into 3 steps of initial, intermediate and final stages according to pH changes and the rates of fermentation and color change were calculated from intermediate stage. The time reached equilibrium in hardness of radish and salt concentration and their equilibrated values were also compared. Fermentation rate became more rapid as fermentation temperature and salt concentration increased. The rates of Hunter color change of Dongchimi liquid were increased as the temperature increased. The 'L' values decreased slowly, and 'a' and 'b' values increased rapidly to maximum at the intermediate stage. The time required to reach equilibrium was reduced for both salt concentration and hardness of radish as the fermentation temperature increased. The equilibrated values of salt concentration were increased while those of hardness of radish were decreased as the temperature and salt concentration increased. The data obtained can be used for prediction of some of the major characteristics of Dongchimi during fermentation.

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Analysis of Crop Survey Protocols to Support Parameter Calibration and Verification for Crop Models of Major Vegetables (주요 채소 작물 대상 작물 모형 모수 추정 및 검증을 지원하기 위한 생육 조사 프로토콜 분석)

  • Kim, Kwang Soo;Kim, Junhwan;Hyun, Shinwoo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.2
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    • pp.68-78
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    • 2020
  • Crop models have been used to predict vegetable crop yield, which would have a considerable economic impact on consumers as well as producers. A small number of models have been developed to estimate growth and yield of vegetables due to limited availability of growth observation data in high-quality. In this study, we aimed to analyze the protocols designed for collection of the observation data for major vegetable crops including cabbage, radish, garlic, onion and pepper. We also designed the protocols suitable for development and verification of a vegetable crop growth model. In particular, different measures were proposed to improve the existing protocol used by Statistics Korea (KOSTAT) and Rural Development Administration (RDA), which would enhance reliability of parameter estimation for the crop model. It would be advantageous to select sampling sites in areas where reliable weather observation data can be obtained because crop models quantify the response of crop growth to given weather conditions. It is recommended to choose multiple sampling sites where climate conditions would differ. It is crucial to collect time series data for comparison between observed and simulated crop growth and yield. A crop model can be developed to predict actual yield rather than attainable yield using data for crop damage caused by diseases and pests as well as weather anomalies. A bigdata platform where the observation data are to be shared would facilitate the development of crop models for vegetable crops.

Evaluation for the Antimicrobial, Antioxidant and Antithrombosis Activity of Natural Spices for Fresh-cut yam. (생마 신선편이 제조를 위한 천연 향신료의 항균, 항산화 및 항혈전 활성 평가)

  • Ryu, Hee-Young;Bae, Kyung-Hwa;Kim, Eun-Joo;Park, Sang-Jo;Lee, Bong-Ho;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.17 no.5 s.85
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    • pp.652-657
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    • 2007
  • To make functional fresh-cut yam using natural spices, the water or the methanol extract was prepared from different kinds of natural spices including ginger, wasabi, black pepper, mustard, cinnamon, chinese pepper, garlic, thyme, rosemary, chamomile, and peppermint. The extraction yields were varied as spice sources and extraction solvents used. Among the water extracts, peppermint extract showed strong antithrombosis activity with 26-folds extended thrombin time, and the $IC_{50}s$ of the extracts from cinnamon and rosemary were $61.3{\mu}g/ml\;and\;84.3{\mu}g/ml$ in DPPH scavenging assay, respectively. Whereas, the antimicrobial activity of all water extracts was ignorable. Among the methanol extracts, the extracts from thyme, ginger, and cinnamon showed antimicrobial activity against yam putrefactive bacteria. Strong antioxidative activity was found in the extracts from cinnamon and thyme. Based on these results, the functional fresh-cut yam was prepared using the water extract from peppermint or cinnamon. The antioxidative and antithrombin activities in the fresh-cut yam were maintained at $4^{\circ}C$ for 24 hr. Our results suggest that the natural spices could be applied to production of functional fresh-cut yam.

Effect of Cultivars, Sowing Date and Cropping System on the Development of Soybean Bacterial Pustule in the Field (콩 품종, 파종시기 및 작부체계가 콩 불마름병 발생에 미치는 영향)

  • Hong, Sung-Jun;Kim, Yong-Ki;Shim, Chang-Ki;Kim, Min-Jeong;Park, Jong-Ho;Han, Eun-Jung;Jee, Hyeong-Jin
    • Korean Journal of Organic Agriculture
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    • v.22 no.4
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    • pp.773-787
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    • 2014
  • Bacterial pustule of soybean caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases in many areas where soybeans are grown. This study was carried out to evaluate the effect of cultivars, sowing date and cropping system on the suppression of soybean bacterial pustule in the field. One hundred soybean cultivars were screened for disease resistance against bacterial pustule in naturally infested field. Among them, fourteen cultivars including 'Pureun' were found to be high resistant. And thirty cultivars showed to be moderate resistant(less than 3% of diseased leaf area). When Soybean cultivar 'Taekwang' were sown in four different dates, May 25, June 5, June 15, and June 25, at 10 day-interval in Milyang, the diseased leaf area of bacterial pustule was 23.3%-25.7%, 14.7%-18.0%, 10.7%-12.8%, and 1.0%-2.7%, respectively. The lowest percentage of diseased leaf area was recorded in the plots sown on June 25, whereas the highest percentage of diseased leaf area was recorded in the plot sown on May 25. As sowing time was delayed, incidence of soybean bacterial pustule found to be comparatively reduced. From December in 2006 to June in 2007, we surveyed the pathogen population of soybean bacterial pustule in five cropping upland soils where soybean was cultivated. The survey result showed the bacterial pustule pathogens were detected from the all cropping soils. The pathogen populations of soybean bacterial pustule in soybean-barley and soybean-garlic cropping soil were significantly lower than that of the other cropping soils. In addition, the incidence of soybean bacterial pustule was decreased under the two cropping systems.

Processing of Sausage Using Duck Mechanically Deboned Meat (오리 기계발골육을 이용한 Sausage 제조)

  • 강동수;최옥수;박욱민
    • Journal of Life Science
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    • v.12 no.1
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    • pp.8-15
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    • 2002
  • In this paper we dealt with processing of sausage using duck mechanically deboned meat(duck-MDM). The results may be summarized as follows : 1) after semi-thawing of freezing duck-MDM at $25^{\circ}C$ for 3 hours and cutting as thin, 2) alkali washing at low temperature for 4 hours by 0.2% NaHCO$_3$and 0.15% NaCl, 3) curing at low temperature for 4 hours, 4) after washing and dehydrating(moisture 80%), 5) grinding at low temperature for 55 minutes by silent cutter such as 1st grinding for 10 minutes added only dehydrated meat, 2rd grinding for 30 minutes added salt in 1st grinded meat and 3rd grinding for 15 minutes added other additives, 6) after quick casing in PVDC film and heating at 9$0^{\circ}C$ for 80 minutes, 7) cooling to below room temperature. The additives added at 3rd grinding process were Polymix-CA(0.3%), Polymix-CS(0.3%), polyphosphate(0.3%), sugar(4.2%), potato starch(8.0%), pyre-phosphates(0.3%), isolated soy protein(7.0%), MSG(0.2%), onion powder(0.5%), garlic powder(0.1%), nutmeg (1.5%), potassium sorbate(<0.1%), food red no.40(0.0075%), egg albumin(7.0%) and gluten(3.0%).

Microbial reduction effect of steam heating, UV irradiation, and gamma irradiation on red pepper powder (스팀 가열 및 자외선, 감마선 조사 처리에 따른 고춧가루의 미생물 저감화 효과)

  • Choi, Jun-Bong;Cheon, Hee Soon;Chung, Myong-Soo;Cho, Won-Il
    • Korean Journal of Food Science and Technology
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    • v.52 no.2
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    • pp.177-182
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    • 2020
  • This study evaluated the effect of steam heating, gamma irradiation, and ultraviolet (UV) irradiation on microorganism reduction in order to determine an effective sterilization method for red pepper powder. The effect of each treatment on the reduction of thermoduric bacteria and total aerobic bacteria in red pepper powder were as follows: 10 kGy gamma irradiation, reduction of 4 log and 6 log CFU/g, respectively; 12 mW/㎠ UV irradiation (264 nm UV-C), reduction of less than 1 log CFU/g; steam heating at 120℃ for 40 s, reduction of approximately 2 log CFU/g. High-temperature short-time processing at 110℃ for 30 s reduced the total bacterial count in Gochujang solution from 5.70 log CFU/g to 2.26 log CFU/g; at 121℃, the solution was commercially sterile. Steam heating resulted in 1, 2, and 4 log microbial inactivation in garlic, onion, and pepper powder, respectively. Steam sterilization, which consumers prefer over other methods, may be an effective method for reducing microorganisms in spice powders, including those in red pepper powder.

Contents of Nitrate and Nitrite in Vegetables and fruits (채소류와 과칠류 중의 질산염 및 아질산염의 함량)

  • Shin, Jung-Hye;Kang, Min-Jung;Yang, Seung-Mi;Kim, Hyung-Sik;Sung, Nak-Ju
    • Journal of Food Hygiene and Safety
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    • v.17 no.2
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    • pp.101-105
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    • 2002
  • A total of 53 vegetables and fruits, consisting of 23 leaf vegetables, 4 fruits vegetables, 4 edible roots,7 spice vegetables and 15 fruits were analyzed for contents of nitrate and nitrite by ion chromatography. Nitrite was detected 10 of 23 kinds of leaf vegetables, which was dominant in lettuce by average 349.9 mg/kg. Nitrate contents of leaf vegetables were average 578.3 mg/kg, 415.7 mg/kg, 348.6 mg/kg in wild plant, mustard leaf and chinese vegetable, respectively. Nitrite was not detected in fruits vegetables and rootcrops but the nitrate contents were abundant and the highest in squash(average, 86.2 mg/kg) and radish(average, 297.5 mg/kg), respectively. In spice vegetables, mate contents were from 0 to 29.8 mg/kg, but not detected in garlic. In welsh onion, its nitrite were higher in small type than large type, while nitrate contents were higher 3 times in the latter. Nitrate contents of fruits were lower in apple(average, 0.5 mg/kg) and higher in plum(average, 76.6 mg/kg) than other samples.

A Survey on Elementary School Childrens′ Awareness of and Preference for Kimchi (초등학생의 김치에 대한 의식과 선호 실태에 관한 연구)

  • 한재숙;김혜영;김정숙;서봉순;한준표
    • Korean journal of food and cookery science
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    • v.13 no.3
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    • pp.259-265
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    • 1997
  • The main purpose of this research is to provide a basic knowledge of Kimchi and to improve elementary school childrens' Kimchi intake. We researchers have done statistical analyses of 808 questionnaires completed by elementary school children and their mothers on their awareness of and preference for Kimchi. The results were as follows: About 80.2% of the children regarded Kimchi as one of our nutritious and traditional foods, and 83.8% of the subjects had an affirmative opinion of Kimchi intake. The most well known Kimchi is as follows: Baechu Kimchi, Kkaktugi, Nabak Kimchl, Chonggak Kimchi, Saaennip Kimchi and Buchu Kimchi. The children preferred the peculiar and refreshing taste of Kimchi. Their preference of Kimchi is in the following order: Baechu Kimchi, Skaktugi, Chonggak Kimchi, Nabak Kimchi and Oi Sobagi. And the children also preferred the stems of the cabbage. The children preferred properly fermented and freshly prepared Kimchi. Their favorite ingredients were red pepper powder, Korean radishes, pickled anchovies, sesame leaves, garlic and scallions. The children wanted Kimchi that is less hot and more sweet. The ingredients children wanted to add to Kimchi are pears, cuttlefish, oranges, apples and cucumbers.

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Literature Review of Tangpyeongchae in Cook Books Published in 1700~1960s (1700년대~1960년대 문헌에 나타난 탕평채의 문헌고찰)

  • Lee, Kyong-Ae;Kim, Bo-Ram;Kim, Hyang-Sook;Shin, Mal-Shick
    • Korean journal of food and cookery science
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    • v.28 no.4
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    • pp.489-497
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    • 2012
  • This study was reviewed the changes in main ingredients, seasonings and cooking methods of Tangpyeongchae in Korean cook books and literatures published from the 1700s to the 1960s. The first published books about Tangpyeongchae were in Kosasibijib and Kyongdojabji, written in 1783 and the late 1700s, respectively. Tangpyeongchae, a representative traditional Korean dish that was royal cuisine offered at ritual events in the Chosun Dynasty, was called Cheongpochae in the royal court. It was a dish made by mixing cheongpomuk (mung bean gel), meat, dropwort, mung bean sprout, egg strips and laver. This dish has been seasoned with vinegar, soy sauce, black pepper, garlic, green onion, red pepper, salt, sugar, sesame oil and sesame salt since the early 1900s. Dropwort, egg strips, laver, pine nut (powder), red pepper powder, and red pepper threads were used as garnishes. Tangpyeongchae was made by mixing cheongpomuk with other ingredients and seasonings until the late 1800s. Since the early 1900s Tangpyeongchae has been seasoned first with other ingredients and then mixed cheongpomuk.