• Mycobiology
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• 제35권3호
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• pp.124-128
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• 2007

Ganoderma resinaceum tolerated sodium chloride salt stress within a range of 0 mM till 300 mM. It responded to salt stress with fluctuation in proline formation at different NaCl concentrations. However, the mycelial dry weight, total protein contents and exopolysaccharides did not changed considerably. Increasing sodium chloride concentration led to morphological alteration in fungal mycelia with disappearance of fungal cell wall, plasmolysis, and vacuolation as indicated with electron microscopic examination of the fungal growth.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1240-1247
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• 2006

The present study investigated the influence of the aeration rate and agitation intensity on the production of the mycelial biomass and antioxidative exopolysaccharide (EPS) in Ganoderma resinaceum. In submerged cultures with varying agitation speeds and aeration rates in a stirred-tank reactor, the maximum mycelial biomass and maximum EPS concentration were achieved at 50 rpm and 300 rpm, respectively. Under varying aeration rates, the highest amount of mycelial biomass (18.1 g/l) was accumulated at the lowest aeration rate (0.5 vvm) and the maximum EPS production (3.0 g/l) obtained at 1.0 vvm. A compositional analysis revealed that the five different EPSs were protein-bound heteropolysaccharides, consisting of 87.17-89.22% carbohydrates and 10.78-12.83% proteins. The culture conditions had a striking affect on the carbohydrate composition of the EPS, resulting in different antioxidative activities. All the EPSs showed strong scavenging activities against superoxide and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals, whereas no clear trend in antioxidative activity was observed against hydroxyl radicals and lipid peroxides. Although the precise reason for this difference is still unclear, the high glucose moiety of EPS is probably linked to its broad spectrum of antioxidative activity.

• Journal of Microbiology
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• 제44권2호
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• pp.233-242
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• 2006

The objectives of this study were to optimize submerged culture conditions of a new fungal isolate, Ganorderma resinaceum, and to enhance the production of bioactive mycelial biomass and exopolysaccharides (EPS) by fed-batch culture. The maximum mycelial growth and EPS production in batch culture were achieved in a medium containing 10 g/l glucose, 8 g/l soy peptone, and 5 mM $MnCl_2$ at an initial pH 6.0 and temperature $31^{\circ}C$. After optimization of culture medium and environmental conditions in batch cultures, a fed-batch culture strategy was employed to enhance production of mycelial biomass and EPS. Five different EPS with molecular weights ranging from 53,000 to 5,257,000 g/mole were obtained from either top or bottom fractions of ethanol precipitate of culture filtrate. A fed-batch culture of G. resinaceum led to enhanced production of both mycelial biomass and EPS. The maximum concentrations of mycelial biomass (42.2 g/l) and EPS (4.6 g/l) were obtained when 50 g/l of glucose was fed at day 6 into an initial 10 g/l of glucose medium. It may be worth attempting with other mushroom fermentation processes for enhanced production of mushroom polysaccharides, particularly those with industrial potential.

• 한국균학회지
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• 제27권1호통권88호
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• pp.39-43
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• 1999

불로초의 계통 분류학적 조사를 위해 불로초속 중 8종(Ganoderma lucidum, G. tsugae, G. pfeifferi, G. resinaceum, G. australe-applanatum, G. oregonense, G. neo-japonicum, G. applanatum) 12균주와 out-group 균주로는 Inonotus xeranticus의 rDNA ITS II를 PCR로 증폭하여 염기서열을 비교 조사하였다. 불로초 속 중 ITS II 영역의 길이는 $247{\sim}257\;bp$로 분포하였고 종간 상동성은$70{\sim}100%$ 로 조사되었다. 계통도를 분석한 결과는 5개의 군(cluster)을 형성하였고 G. tsugae는 각각의 G. lucidum과 하나의 clade를 이루어 두 종은 서로 매우 밀접하게 진화된 것으로 사료되었다. 본 조사에서는 국내에서 재배되고 있는 불로초(G. lucidum)는 그 발생지 및 국외 ITS염기서얼 자료와 비교 및 분석할 때 G. tsugae로 하는 것이 타당할 것으로 사료되었다.

• Mycobiology
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• 제30권2호
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• pp.61-64
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• 2002

A Monokaryotic strain G8M without clamp connections was isolated from germinated basidiospore that was obtained from cultivated fruit body. Strain G8M was used as a tester isolate for 'dikaryon-monokaryon mating'(di-mon mating) with the strains of Ganoderma lucidum, G6 and G35(Korean wild strains), G3(Taiwan), G4(Canada), G15(America), G. oregonense G24, G. resinaceum G28, G. oerstedii G23, and G. subamboinense G29. Isolate G8M was compatible to Korean strains G6 and G35, but was incompatible to foreign strains G3, G4, or G15. Compatible reactions between strains were readily observed macroscopically. Clear barrage lines formed between incompatible strains. These clear lines were not apparent in compatible di-mon matings. The Korean strains were morphologically distinct; they did not form any chlamydospores, and stopped growth at $35^{\circ}C$. The strains of G. lucidum from Korea may be considered as different species from Taiwan, Canadian and American cultures.

• 미생물학회지
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• 제32권4호
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• pp.245-251
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• 1994

영지속(Ganoderma)에 속하는 7종 10균주에 대하여 미토콘드리아 DNA의 제한효소 분절양상 비교를 통한 계통분석을 수행하였다. 여러 가지 제한효소들 중 생산된 절편이 충분한 정보를 가지고 있으면서 서로 구별할 수 있는 6가지의 제한효소를 분석에 이용하였다. 절편양상을 설 비교하여 전체 절편중 공통된 절편의 개수를 구하고 이로부터 염기위치당 염기치환율을 구하였으며, 이를 균주간의 진화거리로 계산하여 PHYLIP package의 Neighbor-joining 방법에 이한 계통도를 얻고 그 결과를 고찰하였다. 특이한점은 G. lucidum의 3균주와 G. lobatum 이 유연관계가 많이 있다는 점이다. 이러한 결과는 G. lucidum과 G. lobatum은 종의 다양성으로 인하여 과거부터 복합종으로 취급되어 왔으며 고전적인 영지속의 분류에 문제점이 많이 있음을 시사해 주고 있다. 따라서 영지속의 분류가 진화경로에 바탕을 둔 자연분류가 되기 위해서는 형태분류 뿐만 아니라 배양 분류와 분자생물학적이 sqnstjr등 다양한 기준에 의해서 재고되어야 할 것으로 판단된다.