• 한국식품영양학회지
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• 제16권1호
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• pp.15-21
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• 2003

버섯 균사체로부터 분리한 단백다당체의 Th1 cell 증식효과 및 각종 암세포에 대한 세포독성을 조사하였다. 단백다당체 시료는 영지버섯, 아가리쿠스, 표고버섯, 운지버섯, 그리고 상황버섯을 10$0^{\circ}C$에서 3시간 열수 추출하는 방법으로 조제하였다. Th1 cell의 세포증식을 조사한 결과, 10mg/$m\ell$ 농도에서 모든 시료가 40% 이상 억제하는 효과가 있는 것으로 나타났다. 7 종류의 암세포를 이용한 암세포 생존율을 조사한 결과, 1mg/$m\ell$ 농도에서 P388D1와 L1210에서 아가리쿠스는 2.4%와 39.7%, 표고버섯은 48.4%와 52.5%의 생존율을 보였다. Sarcoma 180으로 복수암을 유발시킨 마우스에게 아가리쿠스와 표고버섯으로부터 추출한 단백다당체를 섭취시킬 경우 생존율이 27~40%까지 유의적으로 증가하는 것으로 나타났다.

• KSBB Journal
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• 제16권3호
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• pp.307-313
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• 2001

The effects of agitaion and aeration on mycelial growth, exo-polysaccharide (EPS) production, and substrate consumption upon the submerged cultivation of G. lucidum were investigated, and the batch kinetics of the EPS fermentation of G. lucidum were interpreted as function of agitation speed and aeration rate. In a 2.6 L jar fermenter system, the optimum agitation speed and aeration rate for EPS production were determined to be 400 rpm and 1.0 vvm, respectively. The maximum production of EPS obtained was 15 g/L. The logistic model for mycelial growth fitted the experimental data better than that determined by the Monod and the two-thirds power models. The Luedeking-Piret equation adequately modelled the kinetic data obtained for product and substrate.

• 한국균학회지
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• 제8권2호
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• pp.107-113
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• 1980

To investigate antitumor components of Korean higher fungi, the carpophores of Ganoderma lucidum (Fr.)Karst. were collected at Gal-mae-ri in Gyeong-gi Province and extracted with 0.1N sodium hydroxide solution. The extract was purified by dialyzing through Visking tube and a dark brownish power was obtained. The antitumor activity of the fraction was tested against sarcoma 180 implanted in mice. The tumor inhibition ratio of the fraction against the tumor was 87.6% in doses of 50 mg/kg/day for the period of ten days. The tumor in five of 15 mice was completely regressed. The antitumor fraction was chemically analyzed and found to be a complex of polysaccharide and protein, from which four monosaccharides and eighteen amino acids were identified.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.110-110
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• 1995

This study was carried out to investigate the dose dependent antifibrotic effects of G009, the water-soluble fraction of polysaccharide extracted from Ganoderma lucidum. The experimental hepatic cirrhosis was induced by bile duct ligation/scission (BDL/S) in rats. BDL/S rats in each group were dosed 0.5, 2, 5 or 10mg/rat/day orally for 4 weeks after the operation. Antifibrotic effects were evaluated by serum biochemical values, hydroxyproline contents, and light microscopical histology.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.109-109
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• 1995

정상 동물군의 GOT값 및 GPT 값이 각각 137.8$\pm$23.4 및 67.4$\pm$10.0 이었고 acetaminophen 투여군은 418.6$\pm$69.1 및 447.4$\pm$7.7로 증가하였으나 G009 투여군은 그 증가가 현저히 억제되었다. 즉 G009 10mg/kg 투여군은 GOT 및 GPT 값이 192.6$\pm$19.2 및 144.8$\pm$28.7에 그쳐 각각 GOT 및 GPT 값의 상승이 80.5% 및 79.6% 억제되었고 20mg/kg 투여군은 90.8% 및 86.6% 억제되었다. 그러나 Licovek 은 50mg/kg의 투여군에서 이와 유사한 효과가 관찰되었다. 또한 간 절편의 조직학적 관찰 결과 acetaminophen 투여 대조군이 간세포 괴사등 심한 조직 괴사를 보인 반면 G009 10mg/kg 및 20mg/kg 농도에서는 간장 손상이 매우 경미함을 확인할 수 있었다.

• 한국균학회지
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• 제23권3호통권74호
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• pp.209-225
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• 1995

영지 균사체로부터 종양 면역 활성이 우수한 다당류를 얻기 위하여 여러 탄소원을 이용하여 균사체를 배양하였다. 배앙된 균사체로부터 ganoderan을 추출하고 이들의 조성과 항암 및 항보체 활성능을 알아보았다. 여러 탄소원은 영지 균사체에 의해 기질로 이용될 수 있으나 ${\beta}-glucan$성 다당류인 ganoderan의 생성에는 상당한 차이를 보였다. 균사체 생육이 우수한 탄소원에서 배양된 균사체의 경우, 비수용성(WI) 다당류의 생성이 증가하였고, 생육이 저하된 탄소원에서는 수용성(WS) ganoderan이 상대적으로 증가하였으며, 포도당을 탄소원으로 사용할 경우 약 $5.2{\sim}16%$가 알칼리 가용성 ganoderan으로 도입되었다. 탄소원에 따라 수획된 ganoderan은 포도당을 주 구성당으로 이루어진 ${\beta}-glucan$성 다당류이며 만노오스, 과당 등이 포함되어 있었으나, 비수용성 ganoderan의 경우 수용성보다 포도당의 함량이 약 $20{\sim}40%$ 더 많이 포함되었으며 약 16개의 아미노산으로 구성된 소량의 단백질이 결합되어 있었다. WI은 glucose가 주 구성당이었으나 WS는 여러 단당들이 상대적으로 많이 포함되어 있는 heteroglucan의 성격을 보였다. 탄소원에 따라 배양된 균사체의 알칼리 추출된 ganoderan 가운데 항암 및 항 보체 활성이 가장 우수한 것은 갈락토오스를 탄소원으로 이용한 ganoderan이었으며 항보체 활성(ITCH 50,%)치가 35.7%였고, 특히 균사체의 생장이 낮은 탄소원에서 수획한 ganoderan이 항보체 활성이 높게 나타났다. Sarcoma 180이 피하이식된 마우스에 20 mg/kg씩 복강 투여하여 항암 활성을 검토한 결과, 항보체 활성실험에서와 마찬가지로 갈락토오스를 탄소원으로 이용한 ganoderan-GAL이 83.6%의 암 저지율을 보여 항암 활성이 가장 높았으며, 이는 약 5%의 단백질이 결합된 ${\beta}-glucan$성 다당류로 55%의 포도당, 13%의 만노오스, 12%의 과당 및 소량의 galactose, xylose, ribose등으로 구성된 분자량 30 kD의 다당류이다.

• 생명과학회지
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• 제24권7호
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• pp.721-727
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• 2014

버섯 다당류 ${\beta}$-glucan의 항암 활성을 확인하기 위하여 영지균사체로부터 단백다당류(GLP)를 분리하고 Sarcoma-180 육종암을 이식시킨 마우스에 복강 투여하여 항암활성을 확인하였다. 육종암이 서혜부에 이식된 마우스에 GLP를 20 mg/kg의 농도로 10일간 복강투여 한 후 30일차에 확인한 결과, Sarcoma-180 육종암은 대조군대비 71.4% 억제되었으며, 마우스의 혈청, 종양조직 및 간조직 내의 TNF-${\alpha}$의 농도는 대조군보다 높게 나타났다. 따라서 GLP는 생체 내 TNF-${\alpha}$의 양적증가를 유도하며, 종양괴사 또는 에폽토시스와 연관된 육종암의 생장억제가 확인되었다. 이때 생장이 억제된 육종암 세포의 미세구조를 관찰한 결과, 상대적으로 큰 핵과 세포의 에폽토시스에서 전형적으로 보여지는 염색질 응축이 관찰되었으며, 핵막은 특징적으로 뭉쳐져 불규칙한 모양을 나타내었다. 따라서 영지에서 분리된 GLP는 종양 세포의 에폽토시스를 유도하여 종양의 성장을 억제하는 것으로 여겨진다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.106-106
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• 1995

A polysaccharide, G009, isolated from Ganoderma lucidum IY009 subjected to investigating on general pharmacology. This material at the large oral doses of 1000 and 2000mg/kg in mice did neither exhibit any abnormal behaviors nor effects on central nervous system. It also had no influences on hexobarbital-induced sleeping time, rotarod test and spontaneous activity test at each oral dose of 1000mg/kg in mice. No effects on the body temperature and on acetic acid induced writhing syndrome in mice were observed with its oral administration at 1000mg/kg, and the convulsions induced by strychnine and pentetrazole were not inhibited at its oral doses of 1000mg/kg in mice. The solution of G009 as given intravenously at the doses of 30 and 60mg/kg in rabbit had no influences on blood pressure and respiration rates and depth. In isolated organs of rat uterus and fundus muscles and guinea-pig ileum and trachea, it did not show any contraction or relaxation at the concentration of 2$\times$10$^{-3}$g/ml, and the contractive actions produced by oxytocin, acetylcholine, serotonin and histamine did not inhibited by the same doses. This material showed no effect on intestinal propulsion test in mice and gastric secretion in rats at the oral doses of 1000mg/kg. However, it is interesting that the material exhibited potent inhibition of acidified aspirin induced gastric damage at the doses of 500 and 1000mg/kg in rats.

• Biomolecules & Therapeutics
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• 제4권1호
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• pp.1-6
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• 1996

In the present study, the antigenic potential of G009, a polysaccharide isolated from Ganoderma lucidum IY009, was determined in BALB/C mice and Hartley guinea pigs. Antigenicity tests, including passive cutaneous anaphylaxis (PCA), active systemic anaphylaxis (ASA) and indirect hemagglutination test (IHA) were performed according to the established guidelines of National Institute of Safety Research. The results were as follows: 1. Mice showed no production of antibodies against G009 sensitized with an adjuvant, aluminum hydroxide gel (alum), when judged by the heterologous PCA test in rats. Meanwhile, antibodies against ovalbumin (OVA) sensitized with alum were clearly detected. 2. In the studies with guinea pigs, both the sensitization of G009 alone and of G009 with complete Freund's adjutant (CFA) did not produce positive reactions in homologous PCA. In the case of ASA, however, G009 alone and G009 with CFA produced positive reactions. 3. No G009 specific reaction was observed in an IHA assay using sera isolated from G009 sensitized mice. These findings suggest that G009 have no antigenicity potential in mice but may have weak antigenicity in guinea pjgs.

• 동의생리병리학회지
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• 제17권2호
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• pp.316-325
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• 2003

In this paper, the effect of Ganopoly(extracts of Ganoderma lucidum) and Ganopoly/C+(70% Ganopoly + 30% chitosan) on cisplastin-induced nephrotoxicity was investigated in Sprague-Dawley rats. A single dose of cisplastin(5 ㎎/㎏) kg) was administered intraperitoneally after pretreatment of saline, Ganopoly and Ganopoly/C+ for 7 days. The nephrotoxicity and renal function were manifestated by the changes of body weight, blood pressure, biochemical changes and solute in urine and plasma. After the treatment of CDDP(cis-dichlorodiamineplatinum), a significant elevation of kidney weight, serum urea, cretinine, urine volume for 24 hours, urine magnesium, and a severe or significant decrease in body weight, blood pressure, creatinine clearance, urine osmolarity, serum albumin, etc. The nephrotoxicity was further confirmed by a significant decrease in glutathione S-transferase(GSH) in urine and kidney homogenate, GSH, glutathione peroxidase(GSH-Px) and catalase in kidney tissue. And also the lipid peroxidation was significantly increased in kidney homogenate. These signs of nephrotoxicity was ameliorated by the pretreatment and consecutive administration of Ganopoly and Ganopoly/C+ for 14 days after the Lp. injection of CDDP on 7th day after pretreatment of Ganopoly and Ganopoly/C+. The amelioration of nephrotoxicity was evidenced by significant reduction in serum urea and creatinine concentration, and improvement of other index of renal function. And The activity of antioxidant enzymes were partially recovered in kidney tissue of rats treated by CDDP and the administration of Ganopoly and Ganopoly/C+. These results indicate the cispastin induced nephrotoxicity is due to an impairment of tubular reabsorption systems enhanced by necrosis of proximal tubule, and the Ganopoly and Ganopoly/C+ has a partial protective effect on nephrotoxicity induced by CDDP. The polysacchride of Ganoderma lucidum may improve the therapeutic index of nephrotoxicity induced by CDDP. However, it is needed to elucidate the mechanism for confirming the therapeutic effect.