• Title/Summary/Keyword: Ganoderma Iucidum

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Morphological Characterization and Culture Conditions of A White Mutant of Ganoderma Iucidum (영지버섯 백색 변이주의 형태적 특성 및 최적 배양조건)

  • 조수묵;서건식;유승헌;유익동;신관철
    • Microbiology and Biotechnology Letters
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    • v.21 no.6
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    • pp.520-526
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    • 1993
  • A morphologically different form of Ganoderma lucidum was isolated from a cultivator's farm, and its optimum growth conditions were determined. A major difference in their morphology was color of fruit bodies. Fruit bodies of the mutant were white wherase those of normal Ganoderma lucidum were red. Spores of the mutant were global and mycelia were thin. Mycelial growth of this white mutant was favorable on potato sucrose agar medium, and optimum pH of the medium was 5.5.

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Studies on physiological functionality proposal of Coriolus versicolor(Fr.)Quel and Ganoderma Lucidum (Fr.)Karst (구름버섯과 영지버섯의 기능성에 관한 연구)

  • 차은정;황영정;김성훈
    • Korean Journal of Human Ecology
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    • v.7 no.2
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    • pp.31-46
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    • 2004
  • The purpose of this study was to investigate the physiological functionality of Coriolus versicolor Quel (CV) and Ganoderma IUCIdum (Fr.)Karst (GL) by estimate the proximate composition and content of antioxidant components. 1. In the physicochemical property. the content of proximate composition of GL was higher 08.28% of moisture, 10.3% of crude protein, 78.4% of crude fiber) than that of CV, but the content of crude lipid and ashes of GL was higher than that of CV. 2. CV had relatively higher content of antioxidant components such as total phenol, carotenoids and v itamin C. than GL did. Antioxidant mineral components such as magnesium and zinc also had relatively higher in CV than in GL. 3. TBA value, conjugated diene production, LDL oxidation, DPPH of methanol extracts in CV and GL were as high as a-Tocopherol.

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Ganoderma lucidum균 전처리를 이용한 볏짚의 상압.소다펄프화

  • 주용찬;강진하
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.34 no.4
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    • pp.51-60
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    • 2002
  • This study was carried out to develop the biochemical pulping method to enhance energy saving and decrease the capital cost through the soda pulping under atmospheric pressure (100%). Nonwood substrates, rice straw, were pretreated by white-rot fungi, Ganoderma Iucidum. It has acquired several basic data that can be applied in bio-soda pulping. The results of this study were as follow. Without any nutrients or with glucose, N and glucose+N the weight losses of rice straws inoculated by Canoderma Iucidum were 8.5~29.8%, 9.3~32% 11.8~30.1% and 11.8~24.4% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The more extending incubation periods, the more increasing weight losses. The yield of untreated rice saw was 54.8% after pulping. When any nutrients was not added or glucose, N and glucose+N were added for the pretreatment, the total yields were ranged 50.6~38.5%, 48.6~34.4%, 47.2~38.4% and 49.5~42.6% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The yields were gradually decreased based on extending of incubation. The physical properties of rice straw soda pulps without fungal treatment, the density, breaking length, burst index, tear index and folding endurance were 0.24 g/$\textrm{cm}^3$, 2.32 km, 0.91 kPa $m^2$/g, 56.7 mN.$m^2$/g and 35 times, respectively. After pretreatment without any nutrients or with glucose, N and glucose+N as nutrients the density was 0.27~0.30 g/$\textrm{cm}^3$, the breaking length 3.14~5.25 km, burst index 1.42~2.78 kPa.$\textrm{m}^2$/g, tear index45.8~64.5 mN.$\textrm{m}^2$/g and folding endurance 47~288 times at all incubating periods when pulping was done. The physical properties were increased with the increasing incubation periods. However, when glucose+N was added, the physical properties were shown superior results each incubating duration.

Bioactive Substances from Ganoderma lucidum (영지의 생리활성 물질)

  • Bae Woo-Chul;Kim Yong-Seok;Lee Jun-Woo
    • Microbiology and Biotechnology Letters
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    • v.33 no.2
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    • pp.75-83
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    • 2005
  • The popular edible mushroom Ganoderma lucidum was considered the most valuable medicine in ancient Asia and was belived to bring longevity, due to its mysterious power of healing the body and calming the mind. Today, Ganoderma lucidum is still widely revered as a valuable health supplement and herbal medicine worldwide, as studies (mostly conducted in China, Korea, Japan, America) into the medicinal and nutritional values of Ganoderma lucidum revealed that it does indeed contain certain bioactive ingredient (such as polysaccharide, triterpene) that might be benefical for the prevention and treatment of a variety of ailments, including diseases such as hypertension, diabetes, hepatitis, cancer, AIDS.

Correlation between Anticomplementary and Antitumor Activity of the Crude Polysaccaride from Ganoderma Iucidum IY009 (Ganoderma lucidum IY 009조다당 분획들의 항암활성과 항보체활성간의 상호관계)

  • Lee, Kweon-Haeng;Lee, June-Woo;Han, Man-Deuk;Jeong, Hoon;Kim, Young-Il;Oh, Doo-Whan
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.45-51
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    • 1994
  • Antitumor polysaccharides were known to activate complement system and to increase specific serum proteins in mouse, and researcher reported that antitumor activity of polyasccharides might be correlated with their biological properties such as activation of complement system and increase of specific protein $L_{A}$, $L_{B}$ and $L_{C}$ within the mouse serum. In case of several Ganoderma lucidum, there was no correlation between their antitumor activities and their bioloical properties, but the antitumor activities against sarcoma 180 of the alkali extracted crude polysaccharide fractions of the Ganoderma lucidum IY 009. AS, T, AI and M were correlated with their bioloical properties such as anticomplementary activity and intensity of mouse serum protein $L_{A}$, $L_{B}$ and $L_{C}$.

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Utilization of Chitosan-glucan Complex Extracted from Ganoderma Iucidum Wastes as Bioflocculant (생물응집제로서 폐영지박 Chitosan-glucan 복합물의 이용성)

  • 오준현;조홍연;양한철
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.770-776
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    • 1995
  • For the purpose of development of non-toxic and biodegradable flocculant, chitosan complex was isolated from Ganoderma lucidum wastes. The isolated complex was identified as the expected chitosan-glucan complex by IR specta. The complex was extracted by treatment of 50% NaOH solution at 120$\circ$C for 5 hrs, namely optimal condition and solubilized with 2% acetic acid for fur-ther use as flocculant. Preliminary experiments showed that the solubilized complex had higher flocculation activity of 1.3 fold than commercial chitosan at 400 mg/l concentration in soybean curd wastewater. Also the solubilized complex removed 83% of MLSS and 60% of COD in the soybean curd wastewater treated by photosynthetic bacteria, 50% of turbidity and 21% of MLSS in sugar industry wastewater, and 90% of turbidity and 89% of MLSS in alcohol fermentation wastewater. Bacterial cell flocculation activities of the solubilized chitosan-glucan complex were 89% in Bacillus subtilis broth, 81% in Streptococcus lactis broth, and more than 90% in Escherichia coli broth after standing for 2 days. The results reveal that chitosan-glucan complex from Ganoderma lucidum wastes can substitute for commercial chitosan as non-toxic and biodegradable flocculant.

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Immunostimulating Activity by Protoplast Fusants between Ganoderma Iucidum and Lentinus edodes (영지와 표고의 융합체의 면역활성 증강작용)

  • Moon, Chul;Hyun, Jin-Won;Kim, Ha-Won;Shim, Mi-Ja;Kim, Byong-Kak
    • Biomolecules & Therapeutics
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    • v.8 no.2
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    • pp.199-205
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    • 2000
  • On the inter-order protoplast fusants of Lentinus edodes and Ganoderma lucidum was the antitumor activity test performed and the fusant P22 was selected. The hot water extract of the cultured mycelia of P22 were purified by DEAE-cellulose chromatographya and the resulting purified fraction was designated as P22A. It was found to be a proteoglycan whose molecular weight was 47 kDa. When examined for immunopotentiation activity, P22A increased the number of colony forming unit in the bone marrow stem cells to 3-folds. It also potentiated the secretion of nitric oxide in activated macrophages to 2-folds. In humoral immune response, it increased the activities of the alkaline phosphatase in differentiated B cells to 1.6-folds and the number of plaque forming cells to 1.8-folds. In cellular immune response, it restored the depressed response of delayed type hypersensitivity in tumor bearing mice to normal level. These results suggest that P22A have potential to restore the decreased immune activity of the tumor bearing mice to normal level.

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Effects and Batch Kinetics of Agitation and Aeration on Submerged Cultivation of Ganoderma Iucidum (영지의 액체배양에 미치는 통기.교반의 효과와 동력학적 특성)

  • 이학수;정재현;이신영
    • KSBB Journal
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    • v.16 no.3
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    • pp.307-313
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    • 2001
  • The effects of agitaion and aeration on mycelial growth, exo-polysaccharide (EPS) production, and substrate consumption upon the submerged cultivation of G. lucidum were investigated, and the batch kinetics of the EPS fermentation of G. lucidum were interpreted as function of agitation speed and aeration rate. In a 2.6 L jar fermenter system, the optimum agitation speed and aeration rate for EPS production were determined to be 400 rpm and 1.0 vvm, respectively. The maximum production of EPS obtained was 15 g/L. The logistic model for mycelial growth fitted the experimental data better than that determined by the Monod and the two-thirds power models. The Luedeking-Piret equation adequately modelled the kinetic data obtained for product and substrate.

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Antitumor effect of Ganoderma lucidum : Cytotoxicity and Tumor Growth Delayt(1) (영지버섯의 항암효과 :세포독성과 종양의 성장억제에 미치는 영향(1))

  • Kwon, Hyoung-Cheol;Kim, Jung-Soo;Choi, Ki-Chul;Choi, Dong-Seong;Song, Chang-Won
    • Radiation Oncology Journal
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    • v.12 no.3
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    • pp.301-305
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    • 1994
  • Purpose :. To investigate the effect of aqueous extract of Ganoderma lucidum(G.I.) on the surival of tumor cells in vitro and on the growth of tumors in vivo. Materials and Methods : Dried G.I. was made into powder, extracted with distilled water, filtered and diluted from a maximum concentration of 100 mg/ml in sequence. The cytotoxicity of G.I, in vitro was evaluated from its ability to reduce the clonogenicity of SCK tumor cells. For the tumor growth delay study, about $2{\times}10^5$$ of SCK tumor cells were subcutaneously inoculated in the legs of A/J mice. The first experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G.I. from the first day after tomor inoculation for 10 days. The second experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G.I. either once a day for 10 days or twice a day for 5 days beginining from the 7th day after tumor inoculation Results : 1. Cytotoxicity in vitro;survival fraction, as judged from the curve, at G.I. concentration of 0.5, 1,5, 10, 25, 50 and 100 mg/ml were 1.0, $0.74{\pm}0.03$, $0.18{\pm}0.03$, $0.15{\pm}0.02$, $0.006{\pm}0.002$, 0.015 and 0.0015, respectively. 2. Tumor growth delay in vivo; a) the time required for the mean tumor volume to grow to $1,000mm^3$ was 11 days in the control group and 14 days in the experimental group. b) the time required for tumor volume to increase 4 times was 11 days in the control group while it was 10.5 and 12 days in the groups injected with G.I. once a day and twice a day from the 7th day after tumor inoculation respectively. Conclusion : Aqueous extracts of G.I. showed a marked cytotoxicity on the SCK mammary cells in vitro. Tumor growth delay was statistically signiricant when G.I. in-jection was started soon after tumor inoculation, but it was not significant when injection was started after the tumors were firmly established.

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